LOCUS       sequence-484711-        5695 bp    DNA     circular SYN 27-AUG-2025
DEFINITION  Part 1 of biosensor able to detect 13 substituted cinnamic acid 
            based compounds.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Machado LF, Dixon N
  TITLE     Development and substrate specificity screening of an in vivo 
            biosensor for the detection of biomass derived aromatic chemical 
            building blocks.
  JOURNAL   Chem Commun (Camb). 2016 Sep 15;52(76):11402-11405. doi: 
            10.1039/c6cc04559f.
  PUBMED    27722239
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Commun (Camb)"; date: "2016-09-15- 15"; volume: "52"; issue: 
            "76"; pages: "11402-11405"
COMMENT     Sequence Label: pFerCA
FEATURES             Location/Qualifiers
     source          1..5695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1564..1607)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(3941..3984)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      4303..4848
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4749..4768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4965..4982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5374..5476
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(5477..5695,1..441)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5543..5562)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg
      541 acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta
      601 ccggtttatt gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt
      661 ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc
      721 agagcctgat aaaaacggtt agcgcttcgt taatacagat gtaggtgttc cacagggtag
      781 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctt gtttcggcgt
      841 gggtatggtg gcaggccccg tggccggggg actgttgggc gctgccggcc catcgaatgg
      901 tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg
      961 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
     1021 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
     1081 gcgcatatgg gtgaacgtat gcgtcaggat gatcaggatg caggcaccgg tagcaccgat
     1141 gcaccggctg cactgctgcc gagtccgctg gataatatgc tgggttatcg tattcgtcgt
     1201 gcaagcatgg caatgatgca tgatctggct catgcactgc gtccgtttga tattagcgtt
     1261 ggtgaagcaa gcctgctgat tatggttggt gcaaatccgg gttgtcgtca gagcgatctg
     1321 ggtcgtgcac tggaaattaa acgtgcaaat atgacaccgc tggttggtcg tctgaaagca
     1381 cgtggtctga ttacagatgc accgattgat ggtcgtagcc tgagcctgac cctgaccgaa
     1441 gaaggtgaag cactgcagaa agttctgctg gcacatgccc agaccagcaa tgatcgtttt
     1501 cgtaaacgtc tggcactgga tgaaaaagat ctgattgcag caatggcagc actggcacgt
     1561 tgaataaaac gaaaggctca gtcggaagac tgggcctttc gttttattct agaccatcga
     1621 atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt
     1681 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
     1741 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
     1801 ggaagcggcc atggccgttg aagccggtgt tcgtcctcag gcaggcgcac gtgatattaa
     1861 tcgtctgctg cgtccgcgta gcattgcaat tgttggtgca agcgaaacac cgggtagcct
     1921 gggtgcaagc gttctggcaa atctggttcg taatgaattt ccgggtgata ttcatctggt
     1981 taatccgaaa cgtgaaacca ttagcggtcg tccggcagtt ccgagcgttg atgcactgcc
     2041 ggaaggtgtt gattgtgcaa ttctggcaat tccgcgtgtt gcagttctgg ataccatgcg
     2101 tcagctggca gcacgtaaag ccggtgcagc aattatcttt gcagcaggtt ttgccgaagg
     2161 tggtgaacag ggtatggcag atcagcaaga aattggtcgt attgcacatg aagcaggtat
     2221 tgttgttgaa ggtccgaatt gtctgggtag cgttaattat ctggatcgta ttccgctgac
     2281 ctttatcgat accgatatta aagcaccgag tccgggtggt gttggtattg ttagccagag
     2341 cggtgcaatg gcagccgttc tggcagttat gctggaaagc cgtaccctgg atctgaccta
     2401 tagcgttagc accggtaatg aagccggtag cggtgttgaa gattatgtgg aatttatgat
     2461 cgccgatgaa aaaacccgta ttattgccat gattgtggaa cagtttcgtg atccggctcg
     2521 ttttctggca ctgatggata aagcaaatgc agcaggtaaa ctggttgttc tgctgcatcc
     2581 gggtaaaagc agcgcagcac gtgaaagcgc agcaacccat acaggcgcaa tggcaggcga
     2641 ttataaactg atgcgtgcaa aagttgaacg tgccggtgtt gttgttgcag aaaccctgga
     2701 agaactgggt gatattaccg aaattgcagc acgttgtccg gcactgccga gcggtggcac
     2761 agcagttctg ggtgaaagcg gtgcactgaa agcactgaca ctggatctgg cagaagaact
     2821 ggaactggca ctgccgaccc tggatgatgg taatgcaccg gcactgcgtg cagccctgcc
     2881 ggaatttgtt ccggttagca atccgctgga tctgacagca cagggtctgg ttgatccgga
     2941 tatgtattat cgtaccctgg cagcactgtt tgaagatgat cgtgttggca ccatttttgc
     3001 cggtattatt cagaccaatc cggcaaccat tggtattaaa ctgccaccgt ttctgaaagc
     3061 agttcgtgaa ctgaaagcaa ccaaaccggt tatttttggt ggtgttgatg aaggtgcaga
     3121 tgttccggca gattggattg aacagctgcg tgcagaaggt attccgtatt ttccgaccac
     3181 cgaacgtgca ctgcgtgcca ttcgtcgtct gagcgcagcg ggtgcacgtg atgcaagccg
     3241 taccgatgca gcaccggcaa gcgttccggc actggcaagc gaaaaaggtg ttgttccgga
     3301 atataaagca aaagcactgc tggcaccgct gggtattagc tttccgaaag gtcagtttgc
     3361 agcaaccgtt gaagatgcaa ttgcagcagc cgaagcaatt ggtggtccgg ttgttatgaa
     3421 agcacaggca gcagcactga gccataaaag tgatgccggt ggtgttgcac tgaatctggt
     3481 tggtgcggaa gcaattcgtg cagcatggga taaaatgttt gcagatgtga aacgttatga
     3541 cgccagcatt attctggatg gtgttctgat cgaagcaatg ggtgcccgtg gtctggaact
     3601 gattgttggt gccaaaaatg atccgcagtg gggtccggtt attctggcag gttttggtgg
     3661 cgttaccgca gaaattctgc aggatgttcg tctgctgtca ccggatatga ccaaagaagc
     3721 aattgttgca gaactgggta aactgaaaca ggcagcactg ctgcatggtt atcgtggtag
     3781 tccggcactg gatgttggtg cagttgccga actgattggt caggtgggtc gcctgctgcg
     3841 tggcgaaccg cgtattcaag aactggatct gaatccggtt gttgtttatc cggaaggtca
     3901 gggtgcaatt gccctggatg cactgatgct ggttgattga ataaaacgaa aggctcagtc
     3961 ggaagactgg gcctttcgtt ttatggcgcc caatacgcaa accgcctctc cccgcgcgtt
     4021 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4081 gcaacgcaat taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct
     4141 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact aacatgagaa
     4201 ttacaactta tatcgtatgg ggctgacttc aggtgctaca tttgaagaga taaattgcac
     4261 tgaaatctag aaatatttta tctgattaat aagatgatct tcttgagatc gttttggtct
     4321 gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt
     4381 tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc agtcaccaaa
     4441 acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact cctctaaatc
     4501 aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg gactcaagac
     4561 gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca
     4621 gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga caaacgcggc
     4681 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag
     4741 ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt
     4801 gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc
     4861 gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa tctccgcccc
     4921 gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     4981 aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca gccttttttc
     5041 tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt aagccagtat
     5101 acactccgct agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcagta
     5161 gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat
     5221 catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg aataaatacc
     5281 tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa
     5341 gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt
     5401 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     5461 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     5521 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     5581 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     5641 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattc
//
