LOCUS       sequence-484743-        7599 bp    DNA     circular SYN 27-AUG-2025
DEFINITION  Expresses myc-tagged RIG-I K270A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7599)
  TITLE     mvRNA expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3-myc-RIG-I K270A
FEATURES             Location/Qualifiers
     source          1..7599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      919..1507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1408..1427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1661..1678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1795..1816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1831..1836,1837..1854,1855..1861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1869..1885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1874..1896
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1893..1909
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1893..1909
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2023..3201
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2254..3192
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3149..3169
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3273..3302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    6187..6581
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(6892..6909)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6892..6908)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6901..6923)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7117..7136)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7236..7258
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7296..7314)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7382..7486
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7487..7555,7556..7599,1..748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
       61 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      121 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      181 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      241 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      301 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      361 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      421 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      481 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      541 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      601 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      661 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      721 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      781 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      841 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      901 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      961 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1021 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1081 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1141 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1201 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1261 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1321 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1381 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1441 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1501 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1561 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1621 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1681 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1741 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     1801 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     1861 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     1921 ttaattcgag ctcgcccagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt
     1981 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     2041 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     2101 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     2161 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     2221 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     2281 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     2341 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     2401 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     2461 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     2521 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     2581 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     2641 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     2701 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     2761 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     2821 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     2881 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     2941 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     3001 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     3061 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     3121 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     3181 cttccatttc aggtgtcgtg aggaattagc ttgcttacat ttgcttctga cacaactgtg
     3241 ttcactagca acctcaaaca gacaccatgc cggagcagaa gctgatatcc gaggaggacc
     3301 tggccatggg gaccaccgag cagcgacgca gcctgcaagc cttccaggat tatatccgga
     3361 agaccctgga ccctacctac atcctgagct acatggcccc ctggtttagg gaggaagagg
     3421 tgcagtatat tcaggctgag aaaaacaaca agggcccaat ggaggctgcc acactttttc
     3481 tcaagttcct gttggagctc caggaggaag gctggttccg tggctttttg gatgccctag
     3541 accatgcagg ttattctgga ctttatgaag ccattgaaag ttgggatttc aaaaaaattg
     3601 aaaagttgga ggagtataga ttacttttaa aacgtttaca accagaattt aaaaccagaa
     3661 ttatcccaac cgatatcatt tctgatctgt ctgaatgttt aattaatcag gaatgtgaag
     3721 aaattctaca gatttgctct actaagggga tgatggcagg tgcagagaaa ttggtggaat
     3781 gccttctcag atcagacaag gaaaactggc ccaaaacttt gaaacttgct ttggagaaag
     3841 aaaggaacaa gttcagtgaa ctgtggattg tagagaaagg tataaaagat gttgaaacag
     3901 aagatcttga ggataagatg gaaacttctg acatacagat tttctaccaa gaagatccag
     3961 aatgccagaa tcttagtgag aattcatgtc caccttcaga agtgtctgat acaaacttgt
     4021 acagcccatt taaaccaaga aattaccaat tagagcttgc tttgcctgct atgaaaggaa
     4081 aaaacacaat aatatgtgct cctacaggtt gtggagcaac ctttgtttca ctgcttatat
     4141 gtgaacatca tcttaaaaaa ttcccacaag gacaaaaggg gaaagttgtc ttttttgcga
     4201 atcagatccc agtgtatgaa cagcagaaat ctgtattctc aaaatacttt gaaagacatg
     4261 ggtatagagt tacaggcatt tctggagcaa cagctgagaa tgtcccagtg gaacagattg
     4321 ttgagaacaa tgacatcatc attttaactc cacagattct tgtgaacaac cttaaaaagg
     4381 gaacgattcc atcactatcc atctttactt tgatgatatt tgatgaatgc cacaacacta
     4441 gtaaacaaca cccgtacaat atgatcatgt ttaattatct agatcagaaa cttggaggat
     4501 cttcaggccc actgccccag gtcattgggc tgactgcctc ggttggtgtt ggggatgcca
     4561 aaaacacaga tgaagccttg gattatatct gcaagctgtg tgcttctctt gatgcgtcag
     4621 tgatagcaac agtcaaacac aatctggagg aactggagca agttgtttat aagccccaga
     4681 agtttttcag gaaagtggaa tcacggatta gcgacaaatt taaatacatc atagctcagc
     4741 tgatgaggga cacagagagt ctggcaaaga gaatctgcaa agacctcgaa aacttatctc
     4801 aaattcaaaa tagggaattt ggaacacaga aatatgaaca atggattgtt acagttcaga
     4861 aagcatgcat ggtgttccag atgccagaca aagatgaaga gagcaggatt tgtaaagccc
     4921 tgtttttata cacttcacat ttgcggaaat ataatgatgc cctcattatc agtgagcatg
     4981 cacgaatgaa agatgctctg gattacttga aagacttctt cagcaatgtc cgagcagcag
     5041 gattcgatga gattgagcaa gatcttactc agagatttga agaaaagctg caggaactag
     5101 aaagtgtttc cagggatccc agcaatgaga atcctaaact tgaagacctc tgcttcatct
     5161 tacaagaaga gtaccactta aacccagaga caataacaat tctctttgtg aaaaccagag
     5221 cacttgtgga cgctttaaaa aattggattg aaggaaatcc taaactcagt tttctaaaac
     5281 ctggcatatt gactggacgt ggcaaaacaa atcagaacac aggaatgacc ctcccggcac
     5341 agaagtgtat attggatgca ttcaaagcca gtggagatca caatattctg attgccacct
     5401 cagttgctga tgaaggcatt gacattgcac agtgcaatct tgtcatcctt tatgagtatg
     5461 tgggcaatgt catcaaaatg atccaaacca gaggcagagg aagagcaaga ggtagcaagt
     5521 gcttccttct gactagtaat gctggtgtaa ttgaaaaaga acaaataaac atgtacaaag
     5581 aaaaaatgat gaatgactct attttacgcc ttcagacatg ggacgaagca gtatttaggg
     5641 aaaagattct gcatatacag actcatgaaa aattcatcag agatagtcaa gaaaaaccaa
     5701 aacctgtacc tgataaggaa aataaaaaac tgctctgcag aaagtgcaaa gccttggcat
     5761 gttacacagc tgacgtaaga gtgatagagg aatgccatta cactgtgctt ggagatgctt
     5821 ttaaggaatg ctttgtgagt agaccacatc ccaagccaaa gcagttttca agttttgaaa
     5881 aaagagcaaa gatattctgt gcccgacaga actgcagcca tgactgggga atccatgtga
     5941 agtacaagac atttgagatt ccagttataa aaattgaaag ttttgtggtg gaggatattg
     6001 caactggagt tcagacactg tactcgaagt ggaaggactt tcattttgag aagataccat
     6061 ttgatccagc agaaatgtcc aaatgatatc aggtcctcaa tctagaaatt caccccacca
     6121 gtgcaggctg cctatcagaa agtggtggct ggtgtggcta atgccctggc ccacaagtat
     6181 cactaagctc gctttcttgc tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc
     6241 aactactaaa ctgggggata ttatgaaggg ccttgagcat ctggattctg cctaataaaa
     6301 aacatttatt ttcattgcaa tgatgtattt aaattatttc tgaatatttt actaaaaagg
     6361 gaatgtggga ggtcagtgca tttaaaacat aaagaaatga agagctagtt caaaccttgg
     6421 gaaaatacac tatatcttaa actccatgaa agaaggtgag gctgcaaaca gctaatgcac
     6481 attggcaaca gcccctgatg cctatgcctt attcatccct cagaaaagga ttcaagtaga
     6541 ggcttgattt ggaggttaaa gttttgctat gctgtatttt acattactta ttgttttagc
     6601 tgtcctcatg aatgtctttt cactacccat ttgcttatcc tgcatctctc agccttgact
     6661 ccactcagtt ctcttgctta gagataccac ctttcccctg aagtgttcct tccatgtttt
     6721 acggcgagat ggtttctcct cgcctggcca ctcagcctta gttgtctctg ttgtcttata
     6781 gaggtctact tgaagaagga aaaacagggg gcatggtttg actgtcctgt gagcccttct
     6841 tccctgcctc ccccactcac agtgacccgg aatctgcagc ccaagcttgg cactggccgt
     6901 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6961 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7021 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     7081 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     7141 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7201 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7261 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7321 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7381 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7441 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7501 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7561 agaaacgctg gtgaaagtaa aagatgctga agatcagtt
//
