LOCUS       sequence-484956-        2957 bp    DNA     circular SYN 28-AUG-2025
DEFINITION  Expresses influenza A virus synthetic mvRNA NP71.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2957)
  TITLE     mvRNA expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPolI-NP71.1
FEATURES             Location/Qualifiers
     source          1..2957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    737..758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(773..778,779..796,797..803)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    811..827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     816..838
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     835..851
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     835..851
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1332..1349)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1332..1348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1341..1363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1557..1576)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1676..1698
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1736..1754)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1822..1926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1927..1995,1996..2787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2145..2164)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      721 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      781 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      841 acagctatga catgattacg aattcgagct cggtacccgg ggatcctcta gagtcccatt
      901 cgccattacc gaggggacgg tcccctcgga atgttgccca gccggcgcca gcgaggaggc
      961 tgggaccatg ccggccagca aaagcagggt agactagtta ctaggtatac tacctaacta
     1021 gtaaagaaaa atacccttgt ttctactaat aacccggcgg cccaaaatgc cgactcggag
     1081 cgaaagatat acctcccccg gggccgggag gtcgcgtcac cgaccacgcc gccggcccag
     1141 gcgacgcgcg acacggacac ctgtccccaa aaacgccacc atcgcagcca cacacggagc
     1201 gcccggggcc ctctggtcaa ccccaggaca cacgcgggag cagcgccggg ccggggacgc
     1261 cctcccggcc gcccgtgcca cacgcagggg ccggcccgtc tagaattgac ctgcaggcat
     1321 gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1381 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     1441 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     1501 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     1561 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     1621 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1681 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     1741 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1801 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1861 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1921 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1981 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2041 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2101 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2161 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2221 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2281 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2341 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2401 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2461 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2521 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2581 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2641 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2701 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2761 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     2821 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2881 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2941 aagatcaaag gatcttc
//
