LOCUS       sequence-484962-       10968 bp    DNA     circular SYN 28-AUG-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-2xTIR which 
            contain a tandem dimer of TIR domains .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-2xTIR
FEATURES             Location/Qualifiers
     source          1..10968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1826..1828,1829..2542)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1868..1889)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2129..2148)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2476..2497
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2603..3191
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2656..2676)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3074..3085)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3194..3210)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3195..3211)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3301..3534
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3559..3577)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3560..3577)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3867..3886)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4100..4119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4219..4241
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4279..4297)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4365..4469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4470..4538,4539..5330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4688..4707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5501..6089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5990..6009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6243..6260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6330..6350)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6335..6664
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6515..6650
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7798..7863
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7993..8013
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8438..8572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8475..8494)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8529..8548
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8740..9373
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9420..9545
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10042..10275
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10460..10504
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10789..10906
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgccac agactaaaga
      241 gctgggcgga atcacaacac ttgacgaccc tctggggcag accccagaac tgtttgacgc
      301 attcatatgc tactgtccaa acgatataga atttgttcag gagatgatac gccaacttga
      361 gcagactgac tatcgcttga aactttgtgt tagtgaccgg gatgtactcc ccggaacctg
      421 cgtttggagt attgccagtg aattgataga gaaacggtgt cgacggatgg tcgtagtcgt
      481 ctccgacgac taccttcaga gcaaggaatg tgacttccag acaaaattcg ccctttctct
      541 gtcaccaggc gtccaacaga aacgcttgat ccctataaaa tataaggcta tgaaaaaaga
      601 ctttcctagc atactgcggt tcattaccat ctgtgactac accaacccct gcaccaaatc
      661 atggttctgg acacgactcg ctaaggccct cagtctccct ggaggaagtg gtggcagcca
      721 gcagaaccag gagtccgaga agcccttgca agtcgctaga gtagaaagct ccgtccctca
      781 aactaaagag ttgggaggaa ttaccactct tgatgatcca ctcggccaga ctcccgagct
      841 cttcgatgca tttatctgct actgcccaaa cgacattgag ttcgtgcaag agatgatacg
      901 acagctcgaa caaaccgatt accgccttaa gctgtgtgtg agtgatcgag acgttttgcc
      961 tggtacctgc gtttggtcca ttgctagcga gctcatcgaa aagagatgta gaagaatggt
     1021 tgtagtagtc tctgacgatt acctccaatc caaagagtgc gatttccaga ccaagttcgc
     1081 actttcactc tctccaggcg tgcaacaaaa acgactgatc cctatcaagt ataaagctat
     1141 gaagaaggat tttccctcaa tcttgaggtt tattactata tgcgattata ccaatccctg
     1201 cacaaaaagt tggttctgga ccagacttgc caaggctttg tctcttcccg gaggtagtgg
     1261 cgggtcccct ccttctcccc aagaaaactc ctatgtcagc tccactggta gggctcactc
     1321 cggagccgca ccatggcagc cactcgctgc accatcaggt gcttcagccc aggccgcaga
     1381 gcaactgcaa cggggaccca accagccagt cgagtccgac gaaagtcttg gagggctgag
     1441 cgctgctctg cggtcctggc accttacacc aagttgtccc cttgatcctg ctcctctgcg
     1501 ggaggctggg tgcccccaag gtgacacagc aggggaatcc tcatggggtt caggacctgg
     1561 gagccggcca accgctgttg aaggcctggc tttgggctcc tctgcatcat catcatcaga
     1621 acctccccag attatcatca atccagctcg ccagaaaatg gttcagaaat tggctttgta
     1681 tgaggatggt gccctggatt ccttgcaatt gctgtcatct tcttctcttc ccggacttgg
     1741 acttgaacaa gacagacaag gtccagagga gtccgacgag ttccagagcg gaggttccgg
     1801 gggtactggg ggtagtggag gaagtgtgag caagggcgag gagctgttca ccggggtggt
     1861 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1921 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1981 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     2041 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2101 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2161 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2221 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     2281 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     2341 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2401 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     2461 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2521 catggacgag ctgtacaagt aaagcggccg cgactctaga gtcgacctgc aggcatgcaa
     2581 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     2641 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2701 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2761 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     2821 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     2881 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     2941 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3001 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3061 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3121 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3181 cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg gtacctttaa
     3241 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3301 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     3361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
     3541 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
     3601 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
     3661 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
     3721 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
     3781 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     3841 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     3901 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     3961 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     4021 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     4081 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     4141 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4201 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4261 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4321 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4381 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4441 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4501 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4561 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4621 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4681 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4741 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4801 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4861 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4921 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4981 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5041 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5101 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5161 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5221 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5281 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5341 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5401 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5461 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5521 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5581 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5641 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5701 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5761 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5821 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5881 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5941 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6001 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6061 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6121 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6181 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6241 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6301 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6361 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6421 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6481 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6541 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6601 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6661 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     6721 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     6781 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     6841 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     6901 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     6961 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     7021 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     7081 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     7141 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     7201 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     7261 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     7321 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     7381 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     7441 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     7501 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     7561 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     7621 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     7681 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     7741 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     7801 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     7861 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     7921 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     7981 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     8041 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     8101 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     8161 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     8221 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     8281 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     8341 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8401 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     8461 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8521 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     8581 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     8641 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     8701 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     8761 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     8821 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     8881 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     8941 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     9001 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     9061 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     9121 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     9181 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9241 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9301 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9361 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     9421 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     9481 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     9541 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     9601 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     9661 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     9721 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     9781 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     9841 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggn cgctgatctt
     9901 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     9961 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
    10021 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
    10081 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
    10141 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
    10201 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
    10261 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
    10321 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
    10381 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
    10441 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
    10501 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
    10561 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
    10621 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
    10681 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
    10741 agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa
    10801 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    10861 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
    10921 ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcag
//
