LOCUS       sequence-484981-        2957 bp    DNA     circular SYN 02-SEP-2025
DEFINITION  Expresses influenza A virus synthetic mvRNA NP71.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2957)
  TITLE     mvRNA expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPolI-NP71.2_inverted
FEATURES             Location/Qualifiers
     source          1..2957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    67..88
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(103..108,109..126,127..133)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    141..157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     146..168
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     165..181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     165..181
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(662..679)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(662..678)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(671..693)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(887..906)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1006..1028
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1066..1084)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1152..1256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1257..1325,1326..2117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1475..1494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2288..2876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2777..2796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
       61 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      121 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      181 catgattacg aattcgagct cggtacccgg ggatcctcta gagtcccatt cgccattacc
      241 gaggggacgg tcccctcgga atgttgccca gccggcgcca gcgaggaggc tgggaccatg
      301 ccggccagca aaagcagggt agactagtcc ggttgttttg gttgccacta gtaaagaaaa
      361 atacccttgt ttctactaat aacccggcgg cccaaaatgc cgactcggag cgaaagatat
      421 acctcccccg gggccgggag gtcgcgtcac cgaccacgcc gccggcccag gcgacgcgcg
      481 acacggacac ctgtccccaa aaacgccacc atcgcagcca cacacggagc gcccggggcc
      541 ctctggtcaa ccccaggaca cacgcgggag cagcgccggg ccggggacgc cctcccggcc
      601 gcccgtgcca cacgcagggg ccggcccgtc tagaattgac ctgcaggcat gcaagcttgg
      661 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      721 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      781 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
      841 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
      901 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      961 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     1021 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     1081 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1141 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1201 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1261 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1321 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1381 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1441 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1501 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1561 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1621 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1681 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1741 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1801 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1861 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1921 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1981 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2041 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2101 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2161 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2221 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2281 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2341 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2401 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2461 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2521 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2581 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2641 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2701 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2761 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2821 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2881 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2941 ttcctgcgtt atcccct
//
