LOCUS       sequence-485072-       10240 bp    DNA     circular SYN 29-AUG-2025
DEFINITION  CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive 
            plasmid for genome editing in Staphylococcus aureus using S. 
            pyogenes Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Chen W, Zhang Y, Yeo WS, Bae T, Ji Q
  TITLE     Rapid and Efficient Genome Editing in Staphylococcus aureus by Using
            an Engineered CRISPR/Cas9 System.
  JOURNAL   J Am Chem Soc. 2017 Mar 15;139(10):3790-3795. doi: 
            10.1021/jacs.6b13317. Epub 2017 Mar 2.
  PUBMED    28218837
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 29, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.6b13317"; journalName: "J Am Chem Soc"; date: 
            "2017-03-15- 15"; volume: "139"; issue: "10"; pages: "3790-3795"
COMMENT     Sequence Label: pCasSA
FEATURES             Location/Qualifiers
     source          1..10240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     75..93
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(131..153)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     253..272
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     466..488
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     480..497
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     481..497
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3876..3892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3876..3892
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4063..4857
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4117..4136)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4727..4746
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4888..4982
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      5070..5658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5559..5578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_RNA        5796..5871
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             6112..10218
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6112..10215
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ggtaaatacc catgggtatg gacagttttc ccgacgtcta agaaaccatt attatcatga
       61 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
      121 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
      181 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
      241 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
      301 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
      361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      481 gtaaaacgac ggccagtgcc aagcttgcat gcctgcagaa cggattgttg atgattacga
      541 aaatattaag agcacagact attacacaga aaatcaagaa ttaaaaaaac gtagagagag
      601 tttgaaagaa gtagtgaata catggaaaga ggggtatcac gaaaaaagta aagaggttaa
      661 taaattaaag cgagagaatg atagtttgaa tgagcagttg aatgtatcag agaaatttca
      721 agatagtaca gtgactttat atcgtgctgc gagggcgaat ttccctgggt ttgagaaagg
      781 gtttaatagg cttaaagaga aattctttaa tgattccaaa ttcgagcgtg tgggacagtt
      841 tatggatgtt gtacaggata atgtccagaa ggtcgataga aagcgtgaga aacagcgtac
      901 agacgattta gagatgtaga ggtactttta tgccgagaaa actttttgcg tgtgacagtc
      961 cttaaaatat acttagagcg taagcgaaag tagtagcgac agctattaac tttcggttgc
     1021 aaagctctag gatttttaat ggacgcagcg catcacacgc aaaaaggaaa ttggaataaa
     1081 tgcgaaattt gagatgttaa ttaaagacct ttttgaggtc tttttttctt agatttttgg
     1141 ggttatttag gggagaaaac ataggggggt actacgacct cccccctagg tgtccattgt
     1201 ccattgtcca aacaaataaa taaatattgg gtttttaatg ttaaaaggtt gttttttatg
     1261 ttaaagtgaa aaaaacagat gttgggaggt acagtgatgg ttgtagatag aaaagaagag
     1321 aaaaaagttg ctgttacttt aagacttaca acagaagaaa atgagatatt aaatagaatc
     1381 aaagaaaaat ataatattag caaatcagat gcaaccggta ttctaataaa aaaatatgca
     1441 aaggaggaat acggtgcatt ttaaacaaaa aaagatagac agcactggca tgctgcctat
     1501 ctatgactaa attttgttaa atgtattagc accgttatta tatcatgagc gaaaatgtaa
     1561 taaaagaaac tgaaaacaag aaaaattcaa gaggacgtaa ttggacattt gttttatatc
     1621 cagaatcagc aaaagccgag tggttagagt atttaaaaga gttacacatt caatttgtag
     1681 tgtctccatt acatgatagg gatactgata cagaagatag gatgaaaaaa gagcattatc
     1741 atattctagt gatgtatgag ggtaataaat cttatgaaca gataaaaata attacagaag
     1801 aattgaatgc gactattccg cagattgcag gaagtgtgaa aggtcttgtg agatatatgc
     1861 ttcacatgga cgatcctaat aaatttaaat atcaaaaaga agatatgata gtttatggcg
     1921 gtgtagatgt tgatgaatta ttaaagaaaa caacaacaga tagatataaa ttaattaaag
     1981 aaatgattga gtttattgat gaacaaggaa tcgtagaatt taagagttta atggattatg
     2041 caatgaagtt taaatttgat gattggttcc cgcttttatg tgataactcg gcgtatgtta
     2101 ttcaagaata tataaaatca aatcggtata aatctgaccg atagattttg aatttaagag
     2161 tgtcacaaga cactcttttt tcgcaccagc gaaaactggt ttaagccgac tgcgcaaaag
     2221 acataatcga ttcacaaaaa ataggcacac gaaaaacaag ttaagggatg cagtttatgc
     2281 atcccttaac ttacttatta aataatttat agctattgaa aagagataag aattgttcaa
     2341 agctaatatt gtttaaatcg tcaattcctg catgttttaa ggaattgtta aattgatttt
     2401 ttgtaaatat tttcttgtat tctttgttat cttggttacc cgtcttctta atatgcgtaa
     2461 ttgcatctac tctaaatcca tcaatgcctt tatcaaacca ccagttcatc atttcaaata
     2521 cagcatctct aacttccgga ttaccccaat tcaaatcagg ttgtttttta ctgaataaat
     2581 ggaaataata ttgctcagta ttagcatcat attcccgcgc gccaattgag ctcccctttc
     2641 tatgtatgtt ttttactagt catttaaaac gatacattaa taggtacgaa aaagcaactt
     2701 tttttgcgct taaaaccagt cataccaata acttaagggt aactagcctc gccggcaata
     2761 gttaccctta ttatcaagat aagaaagaaa aggatttttc gctacgctca aatcctttaa
     2821 aaaaacacaa aagaccacat tttttaatgt ggtcttttat tcttcaacta aagcacccat
     2881 tagttcaaca aacgaaaatt ggataaagtg ggatattttt aaaatatata tttatgttac
     2941 agtaatattg acttttaaaa aaggattgat tctaatgaag aaagcagaca agtaagcctc
     3001 ctaaattcac tttagataaa aatttaggag gcatatcaaa tgaaatttaa taaaattgat
     3061 ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt
     3121 agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat
     3181 aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt
     3241 agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga gccactttat
     3301 acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac
     3361 ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa
     3421 ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat tccatggact
     3481 tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct acccattatt
     3541 acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta
     3601 catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg
     3661 tcagataggc ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca
     3721 gtcggttttc taatgtcact aacctgcccc gttagttgaa gaaggttttt atattacagc
     3781 tccagatcca tatccttctt tttctgaacc gacttctcct ttttcgcttc tttattccaa
     3841 ttgctttatt gacgttgagc ctcggaaccg gtacccagga aacagctatg accatgtaat
     3901 acgactcact atacggggat atcgtcggaa ttgccagctg gggcgccctc tggtaaggtt
     3961 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     4021 ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     4081 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     4141 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     4201 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     4261 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     4321 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     4381 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     4441 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     4501 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     4561 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     4621 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     4681 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     4741 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     4801 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     4861 ggactctggg gttcgagagc tcgcttggac tcctgttgat agatccagta atgacctcag
     4921 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     4981 atccaagcac tagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5041 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5101 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5161 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5221 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5281 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5341 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5401 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5461 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5521 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5581 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5641 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5701 tggccttttg ctcacatgtt ctttcctgcg ttgtcgacag agtttgcaaa atatacaggg
     5761 gattatatat aatggaaacg agaccattgg tctcagtttt agagctagaa atagcaagtt
     5821 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttga
     5881 gatctgtcca tacccatggt ctagaatgct cgagtcagaa aaatatacct gtatcttttt
     5941 tcaaaagcaa acatgctttg ggtaaacatg taggtattaa cgtcaatgcg acaatagtag
     6001 cattgattaa atgagaatta gtaagtgttt tacttactaa attttattta acctaaaaat
     6061 gaaccacctg gatgtgtggg attaaaaagt gaagagagga ggacatatca catggataag
     6121 aaatactcaa taggcttaga tatcggcaca aatagcgtcg gatgggcggt gatcactgat
     6181 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc
     6241 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt
     6301 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag
     6361 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag
     6421 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta
     6481 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta
     6541 gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag
     6601 tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa
     6661 ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca
     6721 agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa
     6781 aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct
     6841 ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa
     6901 ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga
     6961 gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca
     7021 gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa
     7081 cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt
     7141 ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt
     7201 gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg
     7261 gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg
     7321 acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg
     7381 agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc
     7441 ttgacttttc gaattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca
     7501 tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat
     7561 aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca
     7621 aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa
     7681 ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa
     7741 cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa
     7801 ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt
     7861 gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat
     7921 aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg
     7981 accttatttg aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt
     8041 gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct
     8101 cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt agattttttg
     8161 aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca
     8221 tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat
     8281 attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt
     8341 gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg
     8401 gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga
     8461 atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat
     8521 actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat
     8581 gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatca cattgttcca
     8641 caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat
     8701 cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg
     8761 agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct
     8821 gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa
     8881 actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac
     8941 gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt
     9001 tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat
     9061 gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa
     9121 cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct
     9181 aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg
     9241 aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc
     9301 gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg
     9361 cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc
     9421 ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa
     9481 aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc
     9541 ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta
     9601 ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa
     9661 gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt
     9721 tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga
     9781 aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa
     9841 aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag
     9901 cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca
     9961 gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt
    10021 gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct
    10081 tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta
    10141 gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt
    10201 cagctaggag gtgactgatg gctggttggc gtactgttgt
//
