LOCUS       sequence-485112-        9611 bp    DNA     circular SYN 02-SEP-2025
DEFINITION  Expresses an actuator of Opto-p53 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Tsuruoka T, Goto Y, Aoki K
  TITLE     Opto-p53: A Light-Controllable Activation of p53 Signaling Pathway.
  JOURNAL   Cell Struct Funct. 2025 Jun 12. doi: 10.1247/csf.25017.
  PUBMED    40500178
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2025 Jun 12. doi: 10.1247/csf.25017."
COMMENT     Sequence Label: pPBbsr2-miRFP703-dSal-VP16minADx3-hCRY2-NLS
FEATURES             Location/Qualifiers
     source          1..9611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     gap             1697..1706
                     /estimated_length=10
     intron          1863..2879
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2803..2825
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2887..2906
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2949..3893
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             5559..5579
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5675..6224
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5819..5836)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6046..6065
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6695..6712)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6701..6925
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6972..6988)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6996..7012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7020..7026,7027..7044,7045..7050))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7065..7086
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7320..7382
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7423..7443)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7423..7441)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7462..7478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7486..7502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7510..7516,7517..7534,7535..7540))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7555..7576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7693..7710)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7864..8452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7944..7963)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8623..9414,9415..9483))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9246..9265
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9484..9588)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggggggccc cagggggggc ggggcggggg gggggggggg gggggggggg
     1561 gggggggggg gggggggggg gggagatggg gagagtgaag cagaacgtgg ggttttttgg
     1621 gggggggggg gggggggggc ccctcaaaaa agcaagcccc cggggggggg ggggcggcgc
     1681 ggcgcgcccc ccccccnnnn nnnnnngggg ggcggggggg gggggggggg gggggggggc
     1741 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     1801 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     1861 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     1921 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     1981 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     2041 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     2101 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     2161 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     2221 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     2281 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     2341 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     2401 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     2461 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     2521 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     2581 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     2641 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     2701 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     2761 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     2821 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     2881 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgggatccg
     2941 aattcaccat ggtagcaggt catgcctctg gcagccccgc attcgggacc gcctctcatt
     3001 cgaattgcga acatgaagag atccacctcg ccggctcgat ccagccgcat ggcgcgcttc
     3061 tggtcgtcag cgaacatgat catcgcgtca tccaggccag cgccaacgcc gcggaatttc
     3121 tgaatctcgg aagcgtactc ggcgttccgc tcgccgagat cgacggcgat ctgttgatca
     3181 agatcctgcc gcatctcgat cccaccgccg aaggcatgcc ggtcgcggtg cgctgccgga
     3241 tcggcaatcc ctctacggag tactgcggtc tgatgcatcg gcctccggaa ggcgggctga
     3301 tcatcgaact cgaacgtgcc ggcccgtcga tcgatctgtc aggcacgctg gcgccggcgc
     3361 tggagcggat ccgcacggcg ggttcactgc gcgcgctgtg cgatgacacc gtgctgctgt
     3421 ttcagcagtg caccggctac gaccgggtga tggtgtatcg tttcgatgag caaggccacg
     3481 gcctggtatt ctccgagtgc catgtgcctg ggctcgaatc ctatttcggc aaccgctatc
     3541 cgtcgtcgct ggtcccgcag atggcgcggc agctgtacgt gcggcagcgc gtccgcgtgc
     3601 tggtggacgt cacctatcag ccggtgccgc tggagccgcg gctgtcgccg ctgaccgggc
     3661 gcgatctcga catgtcgggc tgcttcctgc gctcgatgtc gccgatccat ctgcagttcc
     3721 tgaaggacat gggcgtgcgc gccaccctgg cggtgtcgct ggtggtcggc ggcaagctgt
     3781 ggggcctggt tgtctgtcac cattatctgc cgcgcttcat ccgtttcgag ctgcgggcga
     3841 tctgcaaacg gctcgccgaa aggatcgcga cgcggatcac cgcgcttgag agcctcgagg
     3901 gaggatccgg aggttccgca gatgcgctgg acgacttcga ccttgatatg ctccccgccg
     3961 acgctcttga tgacttcgat cttgatatgc tgcctgccga cgctctggat gatttcgact
     4021 tggacatgct ccccggagga tccggaggtt ccggcggccg caagatggac aagaaaacca
     4081 tcgtgtggtt tcggagagat ctgaggattg aggacaaccc agccctggcc gctgccgctc
     4141 acgaaggcag cgtgtttcca gtgttcatct ggtgccccga ggaagaggga cagttctacc
     4201 caggaagggc cagccgctgg tggatgaagc agtccctggc tcacctgtct cagagtctga
     4261 aggccctggg gtcagatctg accctgatca aaacccataa cacaatcagc gccattctgg
     4321 actgtattcg ggtgactgga gccaccaaag tggtgtttaa tcacctgtat gatcccgtga
     4381 gcctggtgcg ggaccataca gtgaaggaga aactggtgga aagaggcatc agtgtgcagt
     4441 catacaacgg ggatctgctg tatgagccat gggaaatcta ctgcgagaag gggaaaccct
     4501 ttaccagctt caattcctac tggaagaaat gtctggacat gtctatcgag agtgtgatgc
     4561 tgccccctcc atggaggctg atgcctatta cagccgctgc cgaagctatc tgggcctgct
     4621 ccattgaaga gctgggactg gaaaacgagg ccgaaaagcc ttctaatgct ctgctgactc
     4681 gcgcctggtc cccaggatgg tctaacgctg ataaactgct gaatgagttc atcgaaaagc
     4741 agctgattga ctatgccaaa aactctaaga aagtggtggg caacagcacc agcctgctgt
     4801 ctccatacct gcactttgga gagatcagtg tgcggcacgt gttccagtgc gctagaatga
     4861 agcagatcat ttgggccagg gataaaaaca gtgagggcga agagtcagcc gacctgtttc
     4921 tgaggggaat cggactgagg gagtactccc gctatatttg tttcaatttt cccttcactc
     4981 acgaacagtc actgctgagc catctgcggt tctttccttg ggacgctgat gtggacaagt
     5041 tcaaagcctg gcggcaggga agaacaggct atcccctggt ggatgctgga atgagagagc
     5101 tgtgggccac tggctggatg cacaatagga tccgcgtgat tgtgagctcc tttgctgtga
     5161 agttcctgct gctgccttgg aagtggggca tgaaatattt ttgggataca ctgctggatg
     5221 ccgacctgga gtgtgacatc ctggggtggc agtacatctc cggatctatt ccagatggcc
     5281 atgaactgga taggctggac aaccccgctc tgcagggcgc taagtatgac cctgagggag
     5341 aatacatcag gcagtggctg cctgagctgg ctaggctgcc aactgaatgg attcaccatc
     5401 cctgggacgc tcctctgaca gtgctgaagg ccagtggggt ggagctggga actaattacg
     5461 ctaaacctat cgtggatatt gacaccgcca gagagctgct ggctaaggcc atctcccgga
     5521 ccagagaagc ccagatcatg attggcgctg cctctagacc taagaagaag cgtaaggtgt
     5581 aaggatccgt cgacgggccg cggtaacaat tgttaactaa cttaagctag caacggtttc
     5641 cctctagcgg gatcaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa
     5701 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5761 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5821 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5881 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5941 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     6001 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     6061 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     6121 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     6181 ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg tcatgaaaac
     6241 atttaacatt tctcaacaag atctagaatt agtagaagta gcgacagaga agattacaat
     6301 gctttatgag gataataaac atcatgtggg agcggcaatt cgtacgaaaa caggagaaat
     6361 catttcggca gtacatattg aagcgtatat aggacgagta actgtttgtg cagaagccat
     6421 tgcgattggt agtgcagttt cgaatggaca aaaggatttt gacacgattg tagctgttag
     6481 acacccttat tctgacgaag tagatagaag tattcgagtg gtaagtcctt gtggtatgtg
     6541 tagggagttg atttcagact atgcaccaga ttgttttgtg ttaatagaaa tgaatggcaa
     6601 gttagtcaaa actacgattg aagaactcat tccactcaaa tatacccgaa attaaaagtc
     6661 gagatgcatc gatgatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     6721 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6781 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6841 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6901 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaacctgcag cccaagcttg
     6961 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7021 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7081 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     7141 atcttaaaag ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa
     7201 taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa
     7261 taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc gataaaacac
     7321 atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag
     7381 ggttaatcta gtatacgcgt ttaattaatc cagcttttgt tccctttagt gagggttaat
     7441 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7501 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7561 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7621 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7681 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7741 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7801 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8281 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8461 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8521 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8581 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8641 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8701 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8761 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8821 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8881 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8941 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9001 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9061 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9121 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9181 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9241 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9301 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9361 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9421 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9481 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9541 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9601 aaaagtgcca c
//
