LOCUS       sequence-485117-        9617 bp    DNA     circular SYN 02-SEP-2025
DEFINITION  Expresses an improved actuator of Opto-p53 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Tsuruoka T, Goto Y, Aoki K
  TITLE     Opto-p53: A Light-Controllable Activation of p53 Signaling Pathway.
  JOURNAL   Cell Struct Funct. 2025 Jun 12. doi: 10.1247/csf.25017.
  PUBMED    40500178
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2025 Jun 12. doi: 10.1247/csf.25017."
COMMENT     Sequence Label: 
            pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-5SD-lin-hCRY2
FEATURES             Location/Qualifiers
     source          1..9617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1668
                     /label=chicken beta-actin promoter
     intron          1669..2685
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2609..2631
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2693..2712
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2757..2777
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2778..2798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2799..2819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2829..3773
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    5681..6230
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5825..5842)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6052..6071
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6701..6718)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6707..6931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6978..6994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7002..7018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7026..7032,7033..7050,7051..7056))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7071..7092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7326..7388
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7429..7449)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7429..7447)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7468..7484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7492..7508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7516..7522,7523..7540,7541..7546))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7561..7582
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7699..7716)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7870..8458)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7950..7969)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8629..9420,9421..9489))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9252..9271
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9490..9594)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1681 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1741 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1801 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     1861 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     1921 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     1981 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     2041 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     2101 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     2161 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     2221 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     2281 gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     2341 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     2401 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     2461 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     2521 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     2581 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     2641 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     2701 ctggttattg tgctgtctca tcattttggc aaagaattgg gatctgtaca accatgccaa
     2761 agaaaaagag aaaagtacca aaaaagaagc gcaaagtgcc gaaaaagaag agaaaggtag
     2821 aattcaccat ggtagcaggt catgcctctg gcagccccgc attcgggacc gcctctcatt
     2881 cgaattgcga acatgaagag atccacctcg ccggctcgat ccagccgcat ggcgcgcttc
     2941 tggtcgtcag cgaacatgat catcgcgtca tccaggccag cgccaacgcc gcggaatttc
     3001 tgaatctcgg aagcgtactc ggcgttccgc tcgccgagat cgacggcgat ctgttgatca
     3061 agatcctgcc gcatctcgat cccaccgccg aaggcatgcc ggtcgcggtg cgctgccgga
     3121 tcggcaatcc ctctacggag tactgcggtc tgatgcatcg gcctccggaa ggcgggctga
     3181 tcatcgaact cgaacgtgcc ggcccgtcga tcgatctgtc aggcacgctg gcgccggcgc
     3241 tggagcggat ccgcacggcg ggttcactgc gcgcgctgtg cgatgacacc gtgctgctgt
     3301 ttcagcagtg caccggctac gaccgggtga tggtgtatcg tttcgatgag caaggccacg
     3361 gcctggtatt ctccgagtgc catgtgcctg ggctcgaatc ctatttcggc aaccgctatc
     3421 cgtcgtcgct ggtcccgcag atggcgcggc agctgtacgt gcggcagcgc gtccgcgtgc
     3481 tggtggacgt cacctatcag ccggtgccgc tggagccgcg gctgtcgccg ctgaccgggc
     3541 gcgatctcga catgtcgggc tgcttcctgc gctcgatgtc gccgatccat ctgcagttcc
     3601 tgaaggacat gggcgtgcgc gccaccctgg cggtgtcgct ggtggtcggc ggcaagctgt
     3661 ggggcctggt tgtctgtcac cattatctgc cgcgcttcat ccgtttcgag ctgcgggcga
     3721 tctgcaaacg gctcgccgaa aggatcgcga cgcggatcac cgcgcttgag agcctcgagg
     3781 ccatggagga gccgcagtca gatcctagcg tcgagccccc tctggatcag gaagattttg
     3841 atgacctatg gaaactactt cctgaaaaca acgttctgga ccccttgccg gaccaagcaa
     3901 tggatgattt gatgctgtcc ccggacgata ttgaacaatg gttcactgaa gacccaggtc
     3961 cagatgaagc tcccagaatg ccagaggctg ctccccccgt ggcccctgca ccagcagctc
     4021 ctacaccggc ggcccctgca ccagccccct cctggcccct gtcatcttct gtcggcggcc
     4081 gcggtggttc cggtggttcc aagatggaca agaaaaccat cgtgtggttt cggagagatc
     4141 tgaggattga ggacaaccca gccctggccg ctgccgctca cgaaggcagc gtgtttccag
     4201 tgttcatctg gtgccccgag gaagagggac agttctaccc aggaagggcc agccgctggt
     4261 ggatgaagca gtccctggct cacctgtctc agagtctgaa ggccctgggg tcagatctga
     4321 ccctgatcaa aacccataac acaatcagcg ccattctgga ctgtattcgg gtgactggag
     4381 ccaccaaagt ggtgtttaat cacctgtatg atcccgtgag cctggtgcgg gaccatacag
     4441 tgaaggagaa actggtggaa agaggcatca gtgtgcagtc atacaacggg gatctgctgt
     4501 atgagccatg ggaaatctac tgcgagaagg ggaaaccctt taccagcttc aattcctact
     4561 ggaagaaatg tctggacatg tctatcgaga gtgtgatgct gccccctcca tggaggctga
     4621 tgcctattac agccgctgcc gaagctatct gggcctgctc cattgaagag ctgggactgg
     4681 aaaacgaggc cgaaaagcct tctaatgctc tgctgactcg cgcctggtcc ccaggatggt
     4741 ctaacgctga taaactgctg aatgagttca tcgaaaagca gctgattgac tatgccaaaa
     4801 actctaagaa agtggtgggc aacagcacca gcctgctgtc tccatacctg cactttggag
     4861 agatcagtgt gcggcacgtg ttccagtgcg ctagaatgaa gcagatcatt tgggccaggg
     4921 ataaaaacag tgagggcgaa gagtcagccg acctgtttct gaggggaatc ggactgaggg
     4981 agtactcccg ctatatttgt ttcaattttc ccttcactca cgaacagtca ctgctgagcc
     5041 atctgcggtt ctttccttgg gacgctgatg tggacaagtt caaagcctgg cggcagggaa
     5101 gaacaggcta tcccctggtg gatgctggaa tgagagagct gtgggccact ggctggatgc
     5161 acaataggat ccgcgtgatt gtgagctcct ttgctgtgaa gttcctgctg ctgccttgga
     5221 agtggggcat gaaatatttt tgggatacac tgctggatgc cgacctggag tgtgacatcc
     5281 tggggtggca gtacatctcc ggatctattc cagatggcca tgaactggat aggctggaca
     5341 accccgctct gcagggcgct aagtatgacc ctgagggaga atacatcagg cagtggctgc
     5401 ctgagctggc taggctgcca actgaatgga ttcaccatcc ctgggacgct cctctgacag
     5461 tgctgaaggc cagtggggtg gagctgggaa ctaattacgc taaacctatc gtggatattg
     5521 acaccgccag agagctgctg gctaaggcca tctcccggac cagagaagcc cagatcatga
     5581 ttggcgctgc ctaagtcgac gggccgcggt aacaattgtt aactaactta agctagcaac
     5641 ggtttccctc tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc
     5701 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     5761 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     5821 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     5881 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     5941 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     6001 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     6061 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     6121 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     6181 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata ccatggtcat
     6241 gaaaacattt aacatttctc aacaagatct agaattagta gaagtagcga cagagaagat
     6301 tacaatgctt tatgaggata ataaacatca tgtgggagcg gcaattcgta cgaaaacagg
     6361 agaaatcatt tcggcagtac atattgaagc gtatatagga cgagtaactg tttgtgcaga
     6421 agccattgcg attggtagtg cagtttcgaa tggacaaaag gattttgaca cgattgtagc
     6481 tgttagacac ccttattctg acgaagtaga tagaagtatt cgagtggtaa gtccttgtgg
     6541 tatgtgtagg gagttgattt cagactatgc accagattgt tttgtgttaa tagaaatgaa
     6601 tggcaagtta gtcaaaacta cgattgaaga actcattcca ctcaaatata cccgaaatta
     6661 aaagtcgaga tgcatcgatg atctagagct cgctgatcag cctcgactgt gccttctagt
     6721 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6781 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6841 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6901 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac ctgcagccca
     6961 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7021 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7081 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7141 cagcggatct taaaagtttt gttactttat agaagaaatt ttgagttttt gttttttttt
     7201 aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa
     7261 atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata
     7321 aaacacatgc gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct
     7381 ttctagggtt aatctagtat acgcgtttaa ttaatccagc ttttgttccc tttagtgagg
     7441 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7501 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7561 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7621 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7681 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7741 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7801 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7861 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7921 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7981 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8041 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8101 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8161 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8221 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8281 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8341 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8401 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8461 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8521 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8581 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8641 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8701 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8761 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8821 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8881 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8941 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9001 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9061 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9121 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9181 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9241 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9301 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9361 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9421 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9481 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9541 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9601 tccccgaaaa gtgccac
//
