LOCUS       sequence-485118-        9597 bp    DNA     circular SYN 02-SEP-2025
DEFINITION  Expresses a negative control of the actuator of Opto-p53 in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Tsuruoka T, Goto Y, Aoki K
  TITLE     Opto-p53: A Light-Controllable Activation of p53 Signaling Pathway.
  JOURNAL   Cell Struct Funct. 2025 Jun 12. doi: 10.1247/csf.25017.
  PUBMED    40500178
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2025 Jun 12. doi: 10.1247/csf.25017."
COMMENT     Sequence Label: 
            pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-L22Q/W23S/W53Q/F54S-lin-hCRY
            2
FEATURES             Location/Qualifiers
     source          1..9597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1648
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     intron          1649..2665
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2589..2611
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2673..2692
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2737..2757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2758..2778
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2779..2799
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2809..3753
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    5661..6210
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5805..5822)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6032..6051
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6681..6698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6687..6911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6958..6974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6982..6998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7006..7012,7013..7030,7031..7036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7051..7072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7306..7368
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7409..7429)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7409..7427)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7448..7464)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7472..7488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7496..7502,7503..7520,7521..7526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7541..7562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7679..7696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7850..8438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7930..7949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8609..9400,9401..9469))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9232..9251
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9470..9574)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     1561 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     1621 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     1681 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1741 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     1801 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     1861 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     1921 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     1981 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     2041 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     2101 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     2161 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     2221 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     2281 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     2341 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     2401 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     2461 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     2521 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     2581 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     2641 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     2701 tcattttggc aaagaattgg gatctgtaca accatgccaa agaaaaagag aaaagtacca
     2761 aaaaagaagc gcaaagtgcc gaaaaagaag agaaaggtag aattcaccat ggtagcaggt
     2821 catgcctctg gcagccccgc attcgggacc gcctctcatt cgaattgcga acatgaagag
     2881 atccacctcg ccggctcgat ccagccgcat ggcgcgcttc tggtcgtcag cgaacatgat
     2941 catcgcgtca tccaggccag cgccaacgcc gcggaatttc tgaatctcgg aagcgtactc
     3001 ggcgttccgc tcgccgagat cgacggcgat ctgttgatca agatcctgcc gcatctcgat
     3061 cccaccgccg aaggcatgcc ggtcgcggtg cgctgccgga tcggcaatcc ctctacggag
     3121 tactgcggtc tgatgcatcg gcctccggaa ggcgggctga tcatcgaact cgaacgtgcc
     3181 ggcccgtcga tcgatctgtc aggcacgctg gcgccggcgc tggagcggat ccgcacggcg
     3241 ggttcactgc gcgcgctgtg cgatgacacc gtgctgctgt ttcagcagtg caccggctac
     3301 gaccgggtga tggtgtatcg tttcgatgag caaggccacg gcctggtatt ctccgagtgc
     3361 catgtgcctg ggctcgaatc ctatttcggc aaccgctatc cgtcgtcgct ggtcccgcag
     3421 atggcgcggc agctgtacgt gcggcagcgc gtccgcgtgc tggtggacgt cacctatcag
     3481 ccggtgccgc tggagccgcg gctgtcgccg ctgaccgggc gcgatctcga catgtcgggc
     3541 tgcttcctgc gctcgatgtc gccgatccat ctgcagttcc tgaaggacat gggcgtgcgc
     3601 gccaccctgg cggtgtcgct ggtggtcggc ggcaagctgt ggggcctggt tgtctgtcac
     3661 cattatctgc cgcgcttcat ccgtttcgag ctgcgggcga tctgcaaacg gctcgccgaa
     3721 aggatcgcga cgcggatcac cgcgcttgag agcctcgagg ccatggagga gccgcagtca
     3781 gatcctagcg tcgagccccc tctgagtcag gaaacatttt cagaccaatc gaaactactt
     3841 cctgaaaaca acgttctgtc ccccttgccg tcccaagcaa tggatgattt gatgctgtcc
     3901 ccggacgata ttgaacaaca gtccactgaa gacccaggtc cagatgaagc tcccagaatg
     3961 ccagaggctg ctccccccgt ggcccctgca ccagcagctc ctacaccggc ggcccctgca
     4021 ccagccccct cctggcccct gtcatcttct gtcggcggcc gcggtggttc cggtggttcc
     4081 aagatggaca agaaaaccat cgtgtggttt cggagagatc tgaggattga ggacaaccca
     4141 gccctggccg ctgccgctca cgaaggcagc gtgtttccag tgttcatctg gtgccccgag
     4201 gaagagggac agttctaccc aggaagggcc agccgctggt ggatgaagca gtccctggct
     4261 cacctgtctc agagtctgaa ggccctgggg tcagatctga ccctgatcaa aacccataac
     4321 acaatcagcg ccattctgga ctgtattcgg gtgactggag ccaccaaagt ggtgtttaat
     4381 cacctgtatg atcccgtgag cctggtgcgg gaccatacag tgaaggagaa actggtggaa
     4441 agaggcatca gtgtgcagtc atacaacggg gatctgctgt atgagccatg ggaaatctac
     4501 tgcgagaagg ggaaaccctt taccagcttc aattcctact ggaagaaatg tctggacatg
     4561 tctatcgaga gtgtgatgct gccccctcca tggaggctga tgcctattac agccgctgcc
     4621 gaagctatct gggcctgctc cattgaagag ctgggactgg aaaacgaggc cgaaaagcct
     4681 tctaatgctc tgctgactcg cgcctggtcc ccaggatggt ctaacgctga taaactgctg
     4741 aatgagttca tcgaaaagca gctgattgac tatgccaaaa actctaagaa agtggtgggc
     4801 aacagcacca gcctgctgtc tccatacctg cactttggag agatcagtgt gcggcacgtg
     4861 ttccagtgcg ctagaatgaa gcagatcatt tgggccaggg ataaaaacag tgagggcgaa
     4921 gagtcagccg acctgtttct gaggggaatc ggactgaggg agtactcccg ctatatttgt
     4981 ttcaattttc ccttcactca cgaacagtca ctgctgagcc atctgcggtt ctttccttgg
     5041 gacgctgatg tggacaagtt caaagcctgg cggcagggaa gaacaggcta tcccctggtg
     5101 gatgctggaa tgagagagct gtgggccact ggctggatgc acaataggat ccgcgtgatt
     5161 gtgagctcct ttgctgtgaa gttcctgctg ctgccttgga agtggggcat gaaatatttt
     5221 tgggatacac tgctggatgc cgacctggag tgtgacatcc tggggtggca gtacatctcc
     5281 ggatctattc cagatggcca tgaactggat aggctggaca accccgctct gcagggcgct
     5341 aagtatgacc ctgagggaga atacatcagg cagtggctgc ctgagctggc taggctgcca
     5401 actgaatgga ttcaccatcc ctgggacgct cctctgacag tgctgaaggc cagtggggtg
     5461 gagctgggaa ctaattacgc taaacctatc gtggatattg acaccgccag agagctgctg
     5521 gctaaggcca tctcccggac cagagaagcc cagatcatga ttggcgctgc ctaagtcgac
     5581 gggccgcggt aacaattgtt aactaactta agctagcaac ggtttccctc tagcgggatc
     5641 aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     5701 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     5761 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     5821 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     5881 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     5941 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     6001 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     6061 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     6121 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     6181 cgtggttttc ctttgaaaaa cacgataata ccatggtcat gaaaacattt aacatttctc
     6241 aacaagatct agaattagta gaagtagcga cagagaagat tacaatgctt tatgaggata
     6301 ataaacatca tgtgggagcg gcaattcgta cgaaaacagg agaaatcatt tcggcagtac
     6361 atattgaagc gtatatagga cgagtaactg tttgtgcaga agccattgcg attggtagtg
     6421 cagtttcgaa tggacaaaag gattttgaca cgattgtagc tgttagacac ccttattctg
     6481 acgaagtaga tagaagtatt cgagtggtaa gtccttgtgg tatgtgtagg gagttgattt
     6541 cagactatgc accagattgt tttgtgttaa tagaaatgaa tggcaagtta gtcaaaacta
     6601 cgattgaaga actcattcca ctcaaatata cccgaaatta aaagtcgaga tgcatcgatg
     6661 atctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6721 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6781 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6841 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6901 gggctctatg gcttctgagg cggaaagaac ctgcagccca agcttggcgt aatcatggtc
     6961 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7021 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7081 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatct taaaagtttt
     7141 gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa
     7201 taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc
     7261 tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta
     7321 cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt aatctagtat
     7381 acgcgtttaa ttaatccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     7441 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7501 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7561 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7621 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7681 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7741 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7801 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7861 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7921 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7981 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8041 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8101 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8161 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8221 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8281 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8341 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8401 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8461 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8521 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8581 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8641 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8701 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8761 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8821 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8881 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8941 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9001 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9061 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9121 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9181 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9241 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9301 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9361 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9421 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9481 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9541 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
