LOCUS       sequence-485119-        9479 bp    DNA     circular SYN 02-SEP-2025
DEFINITION  Expresses an improved localizer of Opto-p53 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Tsuruoka T, Goto Y, Aoki K
  TITLE     Opto-p53: A Light-Controllable Activation of p53 Signaling Pathway.
  JOURNAL   Cell Struct Funct. 2025 Jun 12. doi: 10.1247/csf.25017.
  PUBMED    40500178
REFERENCE   2  (bases 1 to 9479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2025 Jun 12. doi: 10.1247/csf.25017."
COMMENT     Sequence Label: pPBneo-CIBN-lin-p53CT(98-393)-6KQ-mNeonGreen-N...
FEATURES             Location/Qualifiers
     source          1..9479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..27)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     218..237
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     451..473
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     465..482
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     466..482
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     492..511
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        492..510
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   540..574
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        877..1256
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2572..2594
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2656..2675
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2712..3221
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             4143..4850
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4398..4407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4860..4880
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4881..4901
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4902..4922
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5012..5561
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5156..5173)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5383..5402
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5564..6358
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5618..5637)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6228..6247
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6429..6446)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6435..6659
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6706..6722)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6730..6746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6754..6760,6761..6778,6779..6784))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6799..6820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7054..7116
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7157..7177)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7157..7175)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7196..7212)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7220..7236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7244..7250,7251..7268,7269..7274))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7289..7310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7427..7444)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7598..8186)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7678..7697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8357..9148,9149..9217))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8980..8999
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9218..9322)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(9348..9479,1..324))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
       61 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      121 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      181 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      241 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      301 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
      361 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      421 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      481 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg gcgcgccatt ctagattaac
      541 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      601 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      661 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      721 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      781 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      841 tagatatcaa ctagaatgct agcatgggcc catctcgaca ttgattattg actagttatt
      901 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      961 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     1021 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     1081 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     1141 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     1201 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     1261 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     1321 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc
     1381 gcgcccaggg gggggggggg gggggggggg gggcgggcgc gagggggggg gggggggggg
     1441 ggggcgcgcc gggggggggg gggggggggg gggggcgggg gggggggggg gggggggggg
     1501 gggggggggg gggggggggg ggggggggag gtgcggcggc agccaatcag ggcggcgcgc
     1561 tccgaaagtt tccttttagg gcgggggggg gggggcggcg gccctataaa aagcgaggcg
     1621 cgcggcgggc gggggtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
     1681 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     1741 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     1801 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     1861 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     1921 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     1981 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     2041 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
     2101 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
     2161 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
     2221 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc
     2281 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
     2341 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
     2401 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     2461 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     2521 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     2581 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     2641 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     2701 tgggatccac catgaatgga gctataggag gtgacctttt gctcaatttt cctgacatgt
     2761 cggtcctaga gcgccaaagg gctcacctca agtacctcaa tcccaccttt gattctcctc
     2821 tcgccggctt ctttgccgat tcttcaatga ttaccggcgg cgagatggac agctatcttt
     2881 cgactgccgg tttgaatctt ccgatgatgt acggtgagac gacggtggaa ggtgattcaa
     2941 gactctcaat ttcgccggaa acgacgcttg ggactggaaa tttcaaggca gcgaagtttg
     3001 atacagagac taaggattgt aatgaggcgg cgaagaagat gacgatgaac agagatgacc
     3061 tagtagaaga aggagaagaa gagaagtcga aaataacaga gcaaaacaat gggagcacaa
     3121 aaagcatcaa gaagatgaaa cacaaagcca agaaagaaga gaacaatttc tctaatgatt
     3181 catctaaagt gacgaaggaa ttggagaaaa cggattatat tcatggtggt tccggtggtt
     3241 ccgaattccc ttcccagaaa acctaccagg gcagctacgg tttccgtctg ggcttcttgc
     3301 attctgggac agccaagtct gtgacttgca cgtactcccc tgccctcaac aagatgtttt
     3361 gccaactggc caagacctgc cctgtgcagc tgtgggttga ttccacaccc ccgcccggca
     3421 cccgcgtccg cgccatggcc atctacaagc agtcacagca catgacggag gttgtgaggc
     3481 gctgccccca ccatgagcgc tgctcagata gcgatggtct ggcccctcct cagcatctta
     3541 tccgagtgga aggaaatttg cgtgtggagt atttggatga cagaaacact tttcgacata
     3601 gtgtggtggt gccctatgag ccgcctgagg ttggctctga ctgtaccacc atccactaca
     3661 actacatgtg taacagttcc tgcatgggcg gcatgaaccg gaggcccatc ctcaccatca
     3721 tcacactgga agactccagt ggtaatctac tgggacggaa cagctttgag gtgcgtgttt
     3781 gtgcctgtcc tgggagagac cggcgcacag aggaagagaa tctccgcaag aaaggggagc
     3841 ctcaccacga gctgccccca gggagcacta agcgagcact gcccaacaac accagctcct
     3901 ctccccagcc aaagaagaaa ccactggatg gagaatattt cacccttcag atccgtgggc
     3961 gtgagcgctt cgagatgttc cgagagctga atgaggcctt ggaactcaag gatgcccagg
     4021 ctgggaagga gccagggggg agcagggctc actccagcca cctgcagtcc cagcagggtc
     4081 agtctacctc ccgccatcag cagctcatgt tccagacaga agggcctgac tcagacctcg
     4141 agatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca catgagttac
     4201 acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc accggcaatc
     4261 caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc cagttctccc
     4321 cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc taccctgacg
     4381 ggatgtcgcc tttccaggcc gccatggtag atggctccgg ctaccaagtc catcgcacaa
     4441 tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac gagggaagcc
     4501 acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt cctgtgatga
     4561 ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc aacgacaaaa
     4621 ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc taccggagca
     4681 ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg aagaaccagc
     4741 cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc aacttcaagg
     4801 agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag ggcggccgcc
     4861 caaagaaaaa gagaaaagta ccaaaaaaga agagaaaggt accaaaaaag aagagaaagg
     4921 tataagtcga cgggccgcgg taacaattgt taactaactt aagctagcaa cggtttccct
     4981 ctagcgggat caattccgcc ccccccccct aacgttactg gccgaagccg cttggaataa
     5041 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5101 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5161 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5221 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5281 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5341 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5401 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     5461 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     5521 aaccacgggg acgtggtttt cctttgaaaa acacgataat accatgattg aacaagatgg
     5581 attgcatgca ggttcgccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5641 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5701 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     5761 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5821 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5881 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5941 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6001 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6061 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     6121 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6181 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6241 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6301 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaga
     6361 tccaatggag gccgccaccg cggtggagct cgaattaatt catcgatgat ctagagctcg
     6421 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     6481 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     6541 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     6601 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     6661 ttctgaggcg gaaagaacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     6721 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6781 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6841 cgctttccag tcgggaaacc tgtcgtgcca gcggatctta aaagttttgt tactttatag
     6901 aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata aattgtttgt
     6961 tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta ttcaaattaa
     7021 taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg catgattatc
     7081 tttaacgtac gtcacaatat gattatcttt ctagggttaa tctagtatac gcgtttaatt
     7141 aatccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     7201 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7261 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7321 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7381 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7441 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7501 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7621 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7741 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7801 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7861 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7921 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7981 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8041 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8101 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8161 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8221 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8281 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8341 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8401 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8461 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8521 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8581 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8641 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8701 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8761 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8821 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8881 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8941 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9001 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9061 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9121 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9181 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9241 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9301 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     9361 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     9421 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtg
//
