LOCUS       sequence-485121-        9382 bp    DNA     circular SYN 02-SEP-2025
DEFINITION  Expresses a negative control of the localizer of Opto-p53 in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Tsuruoka T, Goto Y, Aoki K
  TITLE     Opto-p53: A Light-Controllable Activation of p53 Signaling Pathway.
  JOURNAL   Cell Struct Funct. 2025 Jun 12. doi: 10.1247/csf.25017.
  PUBMED    40500178
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2025 Jun 12. doi: 10.1247/csf.25017."
COMMENT     Sequence Label: pPBneo-CIBN-lin-p53CT(98-393)-R273H-mNeonGreen...
FEATURES             Location/Qualifiers
     source          1..9382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..27)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     218..237
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     451..473
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     465..482
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     466..482
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     492..511
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        492..510
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   540..574
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        877..1256
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1535..2551
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2475..2497
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2559..2578
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2615..3124
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             4046..4753
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4301..4310
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4763..4783
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4784..4804
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4805..4825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4915..5464
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5059..5076)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5286..5305
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5467..6261
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5521..5540)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6131..6150
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6332..6349)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6338..6562
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6609..6625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6633..6649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6657..6663,6664..6681,6682..6687))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6702..6723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6957..7019
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7060..7080)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7060..7078)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7099..7115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7123..7139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7147..7153,7154..7171,7172..7177))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7192..7213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7330..7347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7501..8089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7581..7600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8260..9051,9052..9120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8883..8902
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9121..9225)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(9251..9382,1..324))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
       61 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      121 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      181 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      241 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      301 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
      361 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      421 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      481 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg gcgcgccatt ctagattaac
      541 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      601 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      661 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      721 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      781 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      841 tagatatcaa ctagaatgct agcatgggcc catctcgaca ttgattattg actagttatt
      901 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      961 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     1021 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     1081 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     1141 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     1201 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     1261 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     1321 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggnsg
     1381 cgcgccrggg gggggggggg ggggsggggg gsggggsggg gsgrggsggr gaggkgcggc
     1441 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
     1501 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
     1561 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
     1621 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
     1681 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
     1741 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
     1801 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
     1861 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
     1921 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     1981 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
     2041 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
     2101 ggggtggcgg caggkggggg tgccgggcgg ggcggggccg ccycgggccg gggrgggctc
     2161 gggggrgggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     2221 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     2281 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     2341 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     2401 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     2461 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     2521 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     2581 gtctcatcat tttggcaaag aattgggatc caccatgaat ggagctatag gaggtgacct
     2641 tttgctcaat tttcctgaca tgtcggtcct agagcgccaa agggctcacc tcaagtacct
     2701 caatcccacc tttgattctc ctctcgccgg cttctttgcc gattcttcaa tgattaccgg
     2761 cggcgagatg gacagctatc tttcgactgc cggtttgaat cttccgatga tgtacggtga
     2821 gacgacggtg gaaggtgatt caagactctc aatttcgccg gaaacgacgc ttgggactgg
     2881 aaatttcaag gcagcgaagt ttgatacaga gactaaggat tgtaatgagg cggcgaagaa
     2941 gatgacgatg aacagagatg acctagtaga agaaggagaa gaagagaagt cgaaaataac
     3001 agagcaaaac aatgggagca caaaaagcat caagaagatg aaacacaaag ccaagaaaga
     3061 agagaacaat ttctctaatg attcatctaa agtgacgaag gaattggaga aaacggatta
     3121 tattcatggt ggttccggtg gttccgaatt cccttcccag aaaacctacc agggcagcta
     3181 cggtttccgt ctgggcttct tgcattctgg gacagccaag tctgtgactt gcacgtactc
     3241 ccctgccctc aacaagatgt tttgccaact ggccaagacc tgccctgtgc agctgtgggt
     3301 tgattccaca cccccgcccg gcacccgcgt ccgcgccatg gccatctaca agcagtcaca
     3361 gcacatgacg gaggttgtga ggcgctgccc ccaccatgag cgctgctcag atagcgatgg
     3421 tctggcccct cctcagcatc ttatccgagt ggaaggaaat ttgcgtgtgg agtatttgga
     3481 tgacagaaac acttttcgac atagtgtggt ggtgccctat gagccgcctg aggttggctc
     3541 tgactgtacc accatccact acaactacat gtgtaacagt tcctgcatgg gcggcatgaa
     3601 ccggaggccc atcctcacca tcatcacact ggaagactcc agtggtaatc tactgggacg
     3661 gaacagcttt gaggtgcatg tttgtgcctg tcctgggaga gaccggcgca cagaggaaga
     3721 gaatctccgc aagaaagggg agcctcacca cgagctgccc ccagggagca ctaagcgagc
     3781 actgcccaac aacaccagct cctctcccca gccaaagaag aaaccactgg atggagaata
     3841 tttcaccctt cagatccgtg ggcgtgagcg cttcgagatg ttccgagagc tgaatgaggc
     3901 cttggaactc aaggatgccc aggctgggaa ggagccaggg gggagcaggg ctcactccag
     3961 ccacctgaag tccaaaaagg gtcagtctac ctcccgccat aaaaaactca tgttcaagac
     4021 agaagggcct gactcagacc tcgagatggt gagcaagggc gaggaggata acatggcctc
     4081 tctcccagcg acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat
     4141 ggtgggtcag ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac
     4201 caagggtgac ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca
     4261 tcagtacctg ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc
     4321 cggctaccaa gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta
     4381 ccgctacacc tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt
     4441 ccctgctgac ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcaggtcgaa
     4501 gaagacttac cccaacgaca aaaccatcat cagtaccttt aagtggagtt acaccactgg
     4561 aaatggcaag cgctaccgga gcactgcgcg gaccacctac acctttgcca agccaatggc
     4621 ggctaactat ctgaagaacc agccgatgta cgtgttccgt aagacggagc tcaagcactc
     4681 caagaccgag ctcaacttca aggagtggca aaaggccttt accgatgtga tgggcatgga
     4741 cgagctgtac aagggcggcc gcccaaagaa aaagagaaaa gtaccaaaaa agaagagaaa
     4801 ggtaccaaaa aagaagagaa aggtataagt cgacgggccg cggtaacaat tgttaactaa
     4861 cttaagctag caacggtttc cctctagcgg gatcaattcc gccccccccc cctaacgtta
     4921 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     4981 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     5041 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     5101 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     5161 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     5221 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     5281 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     5341 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     5401 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     5461 aataccatga ttgaacaaga tggattgcat gcaggttcgc cggccgcttg ggtggagagg
     5521 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5581 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5641 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5701 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5761 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5821 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5881 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5941 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     6001 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     6061 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6121 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6181 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6241 cttcttgacg agttcttctg agatccaatg gaggccgcca ccgcggtgga gctcgaatta
     6301 attcatcgat gatctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     6361 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6421 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6481 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6541 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa cctgcagccc aagcttggcg
     6601 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6661 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6721 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     6781 ttaaaagttt tgttacttta tagaagaaat tttgagtttt tgtttttttt taataaataa
     6841 ataaacataa ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa
     6901 aacttaatat ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg
     6961 cgtcaatttt acgcatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt
     7021 taatctagta tacgcgttta attaatccag cttttgttcc ctttagtgag ggttaattgc
     7081 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7261 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7321 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9241 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     9301 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     9361 tagaccgaga tagggttgag tg
//
