LOCUS       sequence-485125-        7202 bp    DNA     circular SYN 02-SEP-2025
DEFINITION  30x 147-bp Widom 601 nucleosome positioning sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Hammonds EF, Singh A, Suresh KK, Yang S, Zahorodny SSM, Gupta R, 
            Potoyan DA, Banerjee PR, Morrison EA
  TITLE     Histone H3 tail charge patterns govern nucleosome condensate 
            formation and dynamics.
  JOURNAL   bioRxiv [Preprint]. 2025 Apr 10:2025.04.09.647968. doi: 
            10.1101/2025.04.09.647968.
  PUBMED    40291647
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Apr 10:2025.04.09.647968. doi: 
            10.1101/2025.04.09.647968."
COMMENT     Sequence Label: pEM184_30x147bp_Widom601
FEATURES             Location/Qualifiers
     source          1..7202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..810
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(68..87)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      957..1545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1446..1465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1699..1716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1877..1904)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1996..2082)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2131..2153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2145..2162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2146..2162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2172..2191
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2172..2190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6982..6998)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6982..6998)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7035..7055)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7035..7053)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7074..7090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7074..7090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(join(7192..7202,1..9))
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
       61 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
      121 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      181 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
      241 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
      301 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      361 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
      421 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
      481 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
      541 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
      601 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
      661 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
      721 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
      781 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
      841 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
      901 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1621 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1681 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cgcatatgag cgcccaatac
     1741 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     1801 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     1861 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     1921 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     1981 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     2041 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     2101 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
     2161 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacctaacgg agatctgata
     2221 tcgagaatcc cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa
     2281 cgcacgtacg cgctgtcccc cgcgttttaa ccgccaaggg gattactccc tagtctccag
     2341 gcacgtgtca gatatataca tccgatatcg gatctgatat cgagaatccc ggtgccgagg
     2401 ccgctcaatt ggtcgtagac agctctagca ccgcttaaac gcacgtacgc gctgtccccc
     2461 gcgttttaac cgccaagggg attactccct agtctccagg cacgtgtcag atatatacat
     2521 ccgatatcgg atctgatatc gagaatcccg gtgccgaggc cgctcaattg gtcgtagaca
     2581 gctctagcac cgcttaaacg cacgtacgcg ctgtcccccg cgttttaacc gccaagggga
     2641 ttactcccta gtctccaggc acgtgtcaga tatatacatc cgatatcgga tctgatatcg
     2701 agaatcccgg tgccgaggcc gctcaattgg tcgtagacag ctctagcacc gcttaaacgc
     2761 acgtacgcgc tgtcccccgc gttttaaccg ccaaggggat tactccctag tctccaggca
     2821 cgtgtcagat atatacatcc gatatcggat ctgatatcga gaatcccggt gccgaggccg
     2881 ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacgcgct gtcccccgcg
     2941 ttttaaccgc caaggggatt actccctagt ctccaggcac gtgtcagata tatacatccg
     3001 atatcggatc tgatatcgag aatcccggtg ccgaggccgc tcaattggtc gtagacagct
     3061 ctagcaccgc ttaaacgcac gtacgcgctg tcccccgcgt tttaaccgcc aaggggatta
     3121 ctccctagtc tccaggcacg tgtcagatat atacatccga tatcggatct gatatcgaga
     3181 atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg
     3241 tacgcgctgt cccccgcgtt ttaaccgcca aggggattac tccctagtct ccaggcacgt
     3301 gtcagatata tacatccgat atcggatctg atatcgagaa tcccggtgcc gaggccgctc
     3361 aattggtcgt agacagctct agcaccgctt aaacgcacgt acgcgctgtc ccccgcgttt
     3421 taaccgccaa ggggattact ccctagtctc caggcacgtg tcagatatat acatccgata
     3481 tcggatctga tatcgagaat cccggtgccg aggccgctca attggtcgta gacagctcta
     3541 gcaccgctta aacgcacgta cgcgctgtcc cccgcgtttt aaccgccaag gggattactc
     3601 cctagtctcc aggcacgtgt cagatatata catccgatat cggatctgat atcgagaatc
     3661 ccggtgccga ggccgctcaa ttggtcgtag acagctctag caccgcttaa acgcacgtac
     3721 gcgctgtccc ccgcgtttta accgccaagg ggattactcc ctagtctcca ggcacgtgtc
     3781 agatatatac atccgatatc ggatctgata tcgagaatcc cggtgccgag gccgctcaat
     3841 tggtcgtaga cagctctagc accgcttaaa cgcacgtacg cgctgtcccc cgcgttttaa
     3901 ccgccaaggg gattactccc tagtctccag gcacgtgtca gatatataca tccgatatcg
     3961 gatctgatat cgagaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca
     4021 ccgcttaaac gcacgtacgc gctgtccccc gcgttttaac cgccaagggg attactccct
     4081 agtctccagg cacgtgtcag atatatacat ccgatatcgg atctgatatc gagaatcccg
     4141 gtgccgaggc cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgcg
     4201 ctgtcccccg cgttttaacc gccaagggga ttactcccta gtctccaggc acgtgtcaga
     4261 tatatacatc cgatatcgga tctgatatcg agaatcccgg tgccgaggcc gctcaattgg
     4321 tcgtagacag ctctagcacc gcttaaacgc acgtacgcgc tgtcccccgc gttttaaccg
     4381 ccaaggggat tactccctag tctccaggca cgtgtcagat atatacatcc gatatcggat
     4441 ctgatatcga gaatcccggt gccgaggccg ctcaattggt cgtagacagc tctagcaccg
     4501 cttaaacgca cgtacgcgct gtcccccgcg ttttaaccgc caaggggatt actccctagt
     4561 ctccaggcac gtgtcagata tatacatccg atatcggatc tgatatcgag aatcccggtg
     4621 ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac gtacgcgctg
     4681 tcccccgcgt tttaaccgcc aaggggatta ctccctagtc tccaggcacg tgtcagatat
     4741 atacatccga tatcggatct gatatcgaga atcccggtgc cgaggccgct caattggtcg
     4801 tagacagctc tagcaccgct taaacgcacg tacgcgctgt cccccgcgtt ttaaccgcca
     4861 aggggattac tccctagtct ccaggcacgt gtcagatata tacatccgat atcggatctg
     4921 atatcgagaa tcccggtgcc gaggccgctc aattggtcgt agacagctct agcaccgctt
     4981 aaacgcacgt acgcgctgtc ccccgcgttt taaccgccaa ggggattact ccctagtctc
     5041 caggcacgtg tcagatatat acatccgata tcggatctga tatcgagaat cccggtgccg
     5101 aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta cgcgctgtcc
     5161 cccgcgtttt aaccgccaag gggattactc cctagtctcc aggcacgtgt cagatatata
     5221 catccgatat cggatctgat atcgagaatc ccggtgccga ggccgctcaa ttggtcgtag
     5281 acagctctag caccgcttaa acgcacgtac gcgctgtccc ccgcgtttta accgccaagg
     5341 ggattactcc ctagtctcca ggcacgtgtc agatatatac atccgatatc ggatctgata
     5401 tcgagaatcc cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa
     5461 cgcacgtacg cgctgtcccc cgcgttttaa ccgccaaggg gattactccc tagtctccag
     5521 gcacgtgtca gatatataca tccgatatcg gatctgatat cgagaatccc ggtgccgagg
     5581 ccgctcaatt ggtcgtagac agctctagca ccgcttaaac gcacgtacgc gctgtccccc
     5641 gcgttttaac cgccaagggg attactccct agtctccagg cacgtgtcag atatatacat
     5701 ccgatatcgg atctgatatc gagaatcccg gtgccgaggc cgctcaattg gtcgtagaca
     5761 gctctagcac cgcttaaacg cacgtacgcg ctgtcccccg cgttttaacc gccaagggga
     5821 ttactcccta gtctccaggc acgtgtcaga tatatacatc cgatatcgga tctgatatcg
     5881 agaatcccgg tgccgaggcc gctcaattgg tcgtagacag ctctagcacc gcttaaacgc
     5941 acgtacgcgc tgtcccccgc gttttaaccg ccaaggggat tactccctag tctccaggca
     6001 cgtgtcagat atatacatcc gatatcggat ctgatatcga gaatcccggt gccgaggccg
     6061 ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacgcgct gtcccccgcg
     6121 ttttaaccgc caaggggatt actccctagt ctccaggcac gtgtcagata tatacatccg
     6181 atatcggatc tgatatcgag aatcccggtg ccgaggccgc tcaattggtc gtagacagct
     6241 ctagcaccgc ttaaacgcac gtacgcgctg tcccccgcgt tttaaccgcc aaggggatta
     6301 ctccctagtc tccaggcacg tgtcagatat atacatccga tatcggatct gatatcgaga
     6361 atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg
     6421 tacgcgctgt cccccgcgtt ttaaccgcca aggggattac tccctagtct ccaggcacgt
     6481 gtcagatata tacatccgat atcggatctg atatcgagaa tcccggtgcc gaggccgctc
     6541 aattggtcgt agacagctct agcaccgctt aaacgcacgt acgcgctgtc ccccgcgttt
     6601 taaccgccaa ggggattact ccctagtctc caggcacgtg tcagatatat acatccgata
     6661 tcggatctga tatcgagaat cccggtgccg aggccgctca attggtcgta gacagctcta
     6721 gcaccgctta aacgcacgta cgcgctgtcc cccgcgtttt aaccgccaag gggattactc
     6781 cctagtctcc aggcacgtgt cagatatata catccgatat cggatctgat atcgagaatc
     6841 ccggtgccga ggccgctcaa ttggtcgtag acagctctag caccgcttaa acgcacgtac
     6901 gcgctgtccc ccgcgtttta accgccaagg ggattactcc ctagtctcca ggcacgtgtc
     6961 agatatatac atccgatatc ggatccacta gttctagagc ggccgccacc gcggtggagc
     7021 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     7081 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     7141 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     7201 tt
//
