LOCUS       sequence-485173-       16575 bp    DNA     circular SYN 03-SEP-2025
DEFINITION  The plasmid pBBR1-P2-SpCas9 employs pBBR1MCS-2 as the vector, with 
            Cas9 protein derived from S. pyogenes and the stationary phase 
            promoter P2 sourced from S. marcescens HBQA7..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16575)
  TITLE     A CRISPR-Cas9-Based Genetic Editing Tool for Serratia marcescens 
            Using a Stationary Phase Promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBBR1-P2-SpCas9
FEATURES             Location/Qualifiers
     source          1..16575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(1284..1359)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1381..1499)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             1645..2481
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNVSVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             2555..3403
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MVHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMGELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     primer_bind     complement(3415..3431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3424..3446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(4140..4802)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      4803..5574
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             7054..7848
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7108..7127)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7718..7737
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    8253..8274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             8391..12497
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8391..12494
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(12597..13475)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        13502..13786
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     13709..13728
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             13813..13821
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             13830..14246
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             14252..15037
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             15034..15714
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      15715..15959
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     promoter        15960..16405
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(16406..16492,16493..16575,1..1252)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 gcaaaaaaat gaaaaatatc aagtttctga atttgattcg tccacaatta aaaatatctc
       61 ttctgcaaaa ggcctggacg tttgggacag ctggccatta caaaacgctg acggcactgt
      121 cgcaaactat cacggctacc acatcgtctt tgcattagcc ggagatccta aaaatgcgga
      181 tgacacatcg atttacatgt tctatcaaaa agtcggcgaa acttctattg acagctggaa
      241 aaacgctggc cgcgtcttta aagacagcga caaattcgat gcaaatgatt ctatcctaaa
      301 agaccaaaca caagaatggt caggttcagc cacatttaca tctgacggaa aaatccgttt
      361 attctacact gatttctccg gtaaacatta cggcaaacaa acactgacaa ctgcacaagt
      421 taacgtatca gcatcagaca gctctttgaa catcaacggt gtagaggatt ataaatcaat
      481 ctttgacggt gacggaaaaa cgtatcaaaa tgtacagcag ttcatcgatg aaggcaacta
      541 cagctcaggc gacaaccata cgctgagaga tcctcactac gtagaagata aaggccacaa
      601 atacttagta tttgaagcaa acactggaac tgaagatggc taccaaggcg aagaatcttt
      661 atttaacaaa gcatactatg gcaaaagcac atcattcttc cgtcaagaaa gtcaaaaact
      721 tctgcaaagc gataaaaaac gcacggctga gttagcaaac ggcgctctcg gtatgattga
      781 gctaaacgat gattacacac tgaaaaaagt gatgaaaccg ctgattgcat ctaacacagt
      841 aacagatgaa attgaacgcg cgaacgtctt taaaatgaac ggcaaatggt acctgttcac
      901 tgactcccgc ggatcaaaaa tgacgattga cggcattacg tctaacgata tttacatgct
      961 tggttatgtt tctaattctt taactggccc atacaagccg ctgaacaaaa ctggccttgt
     1021 gttaaaaatg gatcttgatc ctaacgatgt aacctttact tactcacact tcgctgtacc
     1081 tcaagcgaaa ggaaacaatg tcgtgattac aagctatatg acaaacagag gattctacgc
     1141 agacaaacaa tcaacgtttg cgccgagctt cctgctgaac atcaaaggca agaaaacatc
     1201 tgttgtcaaa gacagcatcc ttgaacaagg acaattaaca gttaacaaat aaaaacgcaa
     1261 aagaaaatgc cgattcaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
     1321 actagcctta ttttaacttg ctatttctag ctctaaaacg gttggactca agacgatagt
     1381 tacgaccagt ctaaaaagcg cctgaattcg cgaccttctc gttactgaca ggaaaatggg
     1441 ccattggcaa ccagggaaag atgaacgtga tgatgttcac aatttgctga attgtggtga
     1501 tgtgatgctc accgcatttc ctgaaaattc acgctgtatc ttgaaaaatc gacgtttttt
     1561 acgtggtttt ccgtcgaaaa tttaaggtaa gaacctgacc tcgtgattac tatttcgccg
     1621 tgttgacgac atcaggaggc cagtatgacc gtattacata gtgtggattt ttttccgtct
     1681 ggtaacgtgt ccgtggcgat agaaccccgg ctcccgcagg cggattttcc tgaacatcat
     1741 catgattttc atgaaattgt gattgtcgaa catggcacgg gtattcatgt gtttaatggg
     1801 cagccctata ccatcaccgg tggcacggtc tgtttcgtac gcgatcatga tcggcatctg
     1861 tatgaacata ccgataatct gtgtctgacc aatgtgctgt atcgctcgcc ggatcgattt
     1921 cagtttctcg ccgggctgaa tcagttgctg ccacaagagc tggatgggca gtatccgtct
     1981 cactggcgcg ttaaccacag cgtattgcag caggtgcgac agctggttgc acagatggaa
     2041 cagcaggaag gggaaaatga tttaccctcg accgccagtc gcgagatctt gtttatgcaa
     2101 ttactgctct tgctgcgtaa aagcagtttg caggagaacc tggaaaacag cgcatcacgt
     2161 ctcaacttgc ttctggcctg gctggaggac cattttgccg atgaggtgaa ttgggatgcc
     2221 gtggcggatc aattttctct ttcactgcgt acgctacatc ggcagcttaa gcagcaaacg
     2281 ggactgacgc ctcagcgtta cctgaatcgc ctgcgactga tgaaagcccg acatctgcta
     2341 cgccacagtg aggccagcgt tactgacatc gcctatcgct gtggattcag cgacagtaac
     2401 cacttttcga cgctttttcg ccgagagttt aactggtcac cgcgtgatat tcgtcaggga
     2461 cgggatggtt ttctgcaata acgcgaatct tctcaacgta tttgtacgcc atattgcgaa
     2521 taatcaactt cgttctctgg ccgaggtagc cacggtggtg catcagttaa aacttctcaa
     2581 agatgatttt tttgccagcg accagcaggc agtcgctgtg gctgaccgtt atccgcaaga
     2641 tgtctttgcc gaacatacac atgatttttg tgagctggtg attgtctggc gcggtaatgg
     2701 tctgcatgta ctcaacgatc gcccttatcg cattacccgt ggcgatctct tttacattca
     2761 tgctgacgat aaacactcct acgcttccgt taacgatctg gttttgcaga atattattta
     2821 ttgcccagag cggctgaagc tgaatcttga ctggcagggg gcgattccgg gatttagcgc
     2881 cagcgcaggg caaccacact ggcgcttagg tagcgttggg atggcgcagg cgcggcagat
     2941 tatcggtcag cttgagcatg aaagtagtca gcatgtgccg tttgctaacg aaatgggtga
     3001 gttgctgttc gggcagttgg tgatgttgct gaatcgccat cgttacacca gtgattcgtt
     3061 gccgccaaca tccagcgaaa cgttgctgga taagctgatt acccggctgg cggctagcct
     3121 gaaaagtccc tttgcgctgg ataaattttg tgatgaggca tcgtgcagtg agcgcgtttt
     3181 gcgtcagcaa tttcgccagc agactggaat gaccatcaat caatatctgc ggcaggtcag
     3241 agtgtgtcat gcgcaatatc ttctccagca tagccgcctg ttaatcagtg atatttcgac
     3301 cgaatgtggc tttgaagata gtaactattt ttcggtggtg tttacccggg aaaccgggat
     3361 gacgcccagc cagtggcgtc atctcaattc gcagaaagat taattcgcca tgccactggc
     3421 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     3481 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     3541 ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa
     3601 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccgt actgcgatga
     3661 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg
     3721 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac
     3781 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc
     3841 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt
     3901 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag
     3961 agcctgataa aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
     4021 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcga
     4081 ggcggctaca gccgatagtc tggaacagcg cacttacggg ttgctgcgca acccaagtgc
     4141 taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa
     4201 cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg
     4261 gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg gctggctggg cggctcctcg
     4321 ccggggccgg tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg
     4381 ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac
     4441 accgacaggc gcaactggtc gcggggctgg ccccacgcca cgcggtcatt gaccacgtag
     4501 gccgacacgg tgccggggcc gttgagcttc acgacggaga tccagcgctc ggccaccaag
     4561 tccttgactg cgtattggac cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc
     4621 tggctgacca ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg cagcagcatt
     4681 gccgccgtgg gtttcctcgc aataagcccg gcccacgcct catgcgcttt gcgttccgtt
     4741 tgcacccagt gaccgggctt gttcttggct tgaatgccga tttctctgga ctgcgtggcc
     4801 atgcttatct ccatgcggta ggggtgccgc acggttgcgg caccatgcgc aatcagctgc
     4861 aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat
     4921 tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc
     4981 ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg
     5041 gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc
     5101 ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa
     5161 ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg
     5221 gcgggcttcg cccttcgact gcccccactc gcataggctt gggtcgttcc aggcgcgtca
     5281 aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc gccttccact tggtgtccaa
     5341 ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt ccccagagaa aattaaaaaa
     5401 attgatgggg caaggccgca ggccgcgcag ttggagccgg tgggtatgtg gtcgaaggct
     5461 gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc aggcgcagcc tgtccatcag
     5521 cttgtccagc agggttgtcc acgggccgag cgaagcgagc cagccggtgg ccgctcgcgg
     5581 ccatcgtcca catatccacg ggctggcaag ggagcgcagc gaccgcgcag ggcgaagccc
     5641 ggagagcaag cccgtagggc gccgcagccg ccgtaggcgg tcacgacttt gcgaagcaaa
     5701 gtctagtgag tatactcaag cattgagtgg cccgccggag gcaccgcctt gcgctgcccc
     5761 cgtcgagccg gttggacacc aaaagggagg ggcaggcatg gcggcatacg cgatcatgcg
     5821 atgcaagaag ctggcgaaaa tgggcaacgt ggcggccagt ctcaagcacg cctaccgcga
     5881 gcgcgagacg cccaacgctg acgccagcag gacgccagag aacgagcact gggcggccag
     5941 cagcaccgat gaagcgatgg gccgactgcg cgagttgctg ccagagaagc ggcgcaagga
     6001 cgctgtgttg gcggtcgagt acgtcatgac ggccagcccg gaatggtgga agtcggccag
     6061 ccaagaacag caggcggcgt tcttcgagaa ggcgcacaag tggctggcgg acaagtacgg
     6121 ggcggatcgc atcgtgacgg ccagcatcca ccgtgacgaa accagcccgc acatgaccgc
     6181 gttcgtggtg ccgctgacgc aggacggcag gctgtcggcc aaggagttca tcggcaacaa
     6241 agcgcagatg acccgcgacc agaccacgtt tgcggccgct gtggccgatc tagggctgca
     6301 acggggcatc gagggcagca aggcacgtca cacgcgcatt caggcgttct acgaggccct
     6361 ggagcggcca ccagtgggcc acgtcaccat cagcccgcaa gcggtcgagc cacgcgccta
     6421 tgcaccgcag ggattggccg aaaagctggg aatctcaaag cgcgttgaga cgccggaagc
     6481 cgtggccgac cggctgacaa aagcggttcg gcaggggtat gagcctgccc tacaggccgc
     6541 cgcaggagcg cgtgagatgc gcaagaaggc cgatcaagcc caagagacgg cccgagacct
     6601 tcgggagcgc ctgaagcccg ttctggacgc cctggggccg ttgaatcggg atatgcaggc
     6661 caaggccgcc gcgatcatca aggccgtggg cgaaaagctg ctgacggaac agcgggaagt
     6721 ccagcgccag aaacaggccc agcgccagca ggaacgcggg cgcgcacatt tccccgaaaa
     6781 gtgccacctg ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca
     6841 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta
     6901 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     6961 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga
     7021 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     7081 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7141 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7201 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcatgg
     7261 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7321 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7381 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7441 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7501 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7561 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     7621 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7681 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7741 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7801 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     7861 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     7921 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7981 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc ccatgggcaa atattatacg
     8041 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     8101 ttccatgtcg gcagaatgct taatgaatta caacagtttt tatgcatgcg cccaatacgc
     8161 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8221 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8281 ccccaggcaa gcttgaaaaa gtgtgcgatt atcggctatt ctttgagagg cattcgcagg
     8341 ttgtgatcgt cagcacaccg atatttcagg cagacagcgg ggtaacgaga atggataaga
     8401 aatactcaat aggcttagat atcggcacaa atagcgtcgg atgggcggtg atcactgatg
     8461 attataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
     8521 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
     8581 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg
     8641 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt
     8701 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag
     8761 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag
     8821 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt
     8881 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac
     8941 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa
     9001 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa
     9061 atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt
     9121 tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat
     9181 tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg caaattggag
     9241 atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag
     9301 atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac
     9361 gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc
     9421 cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg
     9481 atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg
     9541 atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga
     9601 cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga
     9661 gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct
     9721 tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat
     9781 ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata
     9841 aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa
     9901 atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat
     9961 tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac
    10021 agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat
    10081 taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg
    10141 aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata
    10201 aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga
    10261 ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg
    10321 atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc
    10381 gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta gattttttga
    10441 aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat
    10501 ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata
    10561 ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg
    10621 ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg
    10681 cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa
    10741 tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata
    10801 ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg
    10861 tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatcac attgttccac
    10921 aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc
    10981 gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga
    11041 gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg
    11101 aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa
    11161 ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg
    11221 atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt
    11281 ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg
    11341 cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac
    11401 ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta
    11461 agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga
    11521 acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg
    11581 aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc
    11641 gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta cagacaggcg
    11701 gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa
    11761 aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc
    11821 tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac
    11881 tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag
    11941 ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt
    12001 ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa
    12061 atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa
    12121 agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc
    12181 attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag
    12241 atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg
    12301 aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt
    12361 ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag
    12421 atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc
    12481 agctaggagg tgactgaccc ctcgagggac gaacaataag gcctccctaa cggggggcct
    12541 tttttattga taacaaaatc gacggtatcg atgatatcga attcctgcag cccgggttat
    12601 gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg
    12661 ctggccccgg tgcatttttt aaatacccgc gagaagtaga gttgatcgtc aaaaccaaca
    12721 ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg
    12781 atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt
    12841 gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg
    12901 tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga
    12961 tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc
    13021 gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg
    13081 tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt
    13141 gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga
    13201 taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc
    13261 aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg
    13321 cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg
    13381 agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat
    13441 cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga
    13501 gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc
    13561 ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc
    13621 aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt
    13681 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
    13741 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg
    13801 gaattcgagc tctaaggagg tcataaaaaa tggatattaa tactgaaact gagatcaagc
    13861 aaaagcattc actaaccccc tttcctgttt tcctaatcag cccggcattt cgcgggcgat
    13921 attttcacag ctatttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg
    13981 aggctcagag ctgggcgcgt cactaccagc agctcgcccg tgaagagaaa gaggcagaac
    14041 tggcagacga catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc
    14101 atttgcaacg ccacggggcc agcaaaaaat ccattacccg tgcgtttgat gacgatgttg
    14161 agtttcagga gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc
    14221 aggttgatat tgattcagag gtataaaacg aatgagtact gcactcgcaa cgctggctgg
    14281 gaagctggct gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct
    14341 tcgccagacg gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat tactgatcgt
    14401 tgccaaccag tacggcctta atccgtggac gaaagaaatt tacgcctttc ctgataagca
    14461 gaatggcatc gttccggtgg tgggcgttga tggctggtcc cgcatcatca atgaaaacca
    14521 gcagtttgat ggcatggact ttgagcagga caatgaatcc tgtacatgcc ggatttaccg
    14581 caaggaccgt aatcatccga tctgcgttac cgaatggatg gatgaatgcc gccgcgaacc
    14641 attcaaaact cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat
    14701 gttacgtcat aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta
    14761 tgacaaggat gaagccgagc gcattgtcga aaatactgca tacactgcag aacgtcagcc
    14821 ggaacgcgac atcactccgg ttaacgatga aaccatgcag gagattaaca ctctgctgat
    14881 cgccctggat aaaacatggg atgacgactt attgccgctc tgttcccaga tatttcgccg
    14941 cgacattcgt gcatcgtcag aactgacaca ggccgaagca gtaaaagctc ttggattcct
    15001 gaaacagaaa gccgcagagc agaaggtggc agcatgacac cggacattat cctgcagcgt
    15061 accgggatcg atgtgagagc tgtcgaacag ggggatgatg cgtggcacaa attacggctc
    15121 ggcgtcatca ccgcttcaga agttcacaac gtgatagcaa aaccccgctc cggaaagaag
    15181 tggcctgaca tgaaaatgtc ctacttccac accctgcttg ctgaggtttg caccggtgtg
    15241 gctccggaag ttaacgctaa agcactggcc tggggaaaac agtacgagaa cgacgccaga
    15301 accctgtttg aattcacttc cggcgtgaat gttactgaat ccccgatcat ctatcgcgac
    15361 gaaagtatgc gtaccgcctg ctctcccgat ggtttatgca gtgacggcaa cggccttgaa
    15421 ctgaaatgcc cgtttacctc ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc
    15481 ataaagtcag cttacatggc ccaggtgcag tacagcatgt gggtgacgcg aaaaaatgcc
    15541 tggtactttg ccaactatga cccgcgtatg aagcgtgaag gcctgcatta tgtcgtgatt
    15601 gagcgggatg aaaagtacat ggcgagtttt gacgagatcg tgccggagtt catcgaaaaa
    15661 atggacgagg cactggctga aattggtttt gtatttgggg agcaatggcg atgacgcatc
    15721 ctcacgataa tatccgggta ggcgcaatca ctttcgtcta ctccgttaca aagcgaggct
    15781 gggtatttcc cggcctttct gttatccgaa atccactgaa agcacagcgg ctggctgagg
    15841 agataaataa taaacgaggg gctgtatgca caaagcatct tctgttgagt taagaacgag
    15901 tatcgagatg gcacatagcc ttgctcaaat tggaatcagg tttgtgccaa taccagtagc
    15961 acatatacct gccgttcact attatttagt gaaatgagat attatgatat tttctgaatt
    16021 gtgattaaaa aggcaacttt atgcccatgc aacagaaact ataaaaaata cagagaatga
    16081 aaagaaacag atagattttt tagttcttta ggcccgtagt ctgcaaatcc ttttatgatt
    16141 ttctatcaaa caaaagagga aaatagacca gttgcaatcc aaacgagagt ctaatagaat
    16201 gaggtcgaaa agtaaatcgc gcgggtttgt tactgataaa gcaggcaaga cctaaaatgt
    16261 gtaaagggca aagtgtatac tttggcgtca ccccttacat attttaggtc tttttttatt
    16321 gtgcgtaact aacttgccat cttcaaacag gagggctgga agaagcagac cgctaacaca
    16381 gtacataaaa aaggagacat gaacgatgaa catcaaaaag tttgcaaaac aagcaacagt
    16441 attaaccttt actaccgcac tgctggcagg aggcgcaact caagcgtttg cgaaagaaac
    16501 gaaccaaaag ccatataagg aaacatacgg catttcccat attacacgcc atgatatgct
    16561 gcaaatccct gaaca
//
