LOCUS       sequence-485197-       13601 bp    DNA     circular SYN 03-SEP-2025
DEFINITION  Dox-inducible lentiviral transfer vector with Arg kinase-dead 
            (K281M).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 13601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pINDUCER20-ABL2(K281M)
FEATURES             Location/Qualifiers
     source          1..13601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2399,2400..2418,2419..2441,
                     2442..2460,2461..2483,2484..2502)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2535..2573
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2570..2594
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2703..2727
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3575..3586
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6173..6197)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6250..6276
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        6362..7573
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7410..7429
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7632..8249,8250..8255,8256..8291,8292..8294,8295..8330,
                     8331..8333,8334..8369,8370..8378)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8431..9004
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8598..8615)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8825..8844
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9020..9814
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9020..9814
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9074..9093)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9684..9703
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9991..10579
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10044..10064)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10462..10473)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10654..10887
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11006..11024)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11092..11196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11197..11265,11266..12057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11415..11434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12228..12816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12717..12736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12970..12987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13227..13248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13263..13268,13269..13286,13287..13293)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13301..13317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13306..13328
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13325..13341
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13325..13341
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13391..13410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt gtttaccact
     2401 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2461 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     2521 ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     2581 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     2641 gggaccgatc cagcctccgc ggccccgaac tagtccagtg tggtggaatt ctgcagatat
     2701 caacaagttt gtacaaaaaa gttggcacca tggtccttgg gacagttctc cttccaccta
     2761 atagttatgg cagagatcag gacacttcac tttgctgcct gtgcactgag gcctcagaat
     2821 ctgctctacc cgacttaaca gaagctttgc atcgtcccta tggttgtgat gttgaacccc
     2881 aggcactaaa tgaggctatc aggtggagct ccaaggagaa cttgctcgga gccactgaga
     2941 gtgaccctaa tctcttcgtt gcactttatg attttgtagc aagtggtgat aacacactca
     3001 gcatcactaa aggtgaaaag ctacgagtcc ttggttacaa ccagaatggt gagtggagtg
     3061 aagttcgctc taagaatggg cagggctggg tgccaagcaa ctacatcacc ccagtgaaca
     3121 gcctggaaaa acactcctgg taccatggac ctgtgtcacg cagtgcagct gagtatctgc
     3181 tcagcagtct aatcaatggc agcttcctgg tgcgagaaag tgagagtagc cctgggcagc
     3241 tgtccatctc gctcaggtac gagggacgtg tgtatcacta caggatcaat accactgcag
     3301 atggcaaggt gtatgtgact gctgagagcc gcttcagcac cttggcagag cttgtacacc
     3361 atcactccac agtggctgat gggctggtga caacattaca ctacccagca cccaagtgta
     3421 ataagcctac agtctatggt gtgtccccca tccacgacaa atgggaaatg gagcgaacag
     3481 atattaccat gaagcacaaa cttgggggcg gtcagtatgg agaggtttac gttggcgtct
     3541 ggaagaaata cagccttaca gttgctgtga tgacattgaa ggaagatacc atggaggtag
     3601 aagaattcct gaaagaagct gcagtaatga aggaaatcaa gcatcctaat ctggtacaac
     3661 ttttaggtgt gtgtactttg gagccaccat tttacattgt gactgaatac atgccatacg
     3721 ggaatttgct ggattacctc cgagaatgca accgagaaga ggtgactgca gttgtgctgc
     3781 tctacatggc cactcagatt tcttctgcaa tggagtactt agagaagaag aatttcatcc
     3841 atagagatct tgcagctcgt aactgcctag tgggagaaaa ccatgtggta aaagtggctg
     3901 actttggctt aagtagattg atgactggag acacttatac tgctcatgct ggagccaaat
     3961 ttcctattaa gtggacagca ccagagagtc ttgcctacaa taccttctca attaaatctg
     4021 acgtctgggc ttttggggta ttgttgtggg aaattgctac ctatggaatg tcaccatatc
     4081 caggtattga cctgtctcag gtctatgacc tactagaaaa aggatatcga atggaacagc
     4141 ctgagggatg cccccctaag gtttatgaac ttatgagagc atgctggaag tggagccctg
     4201 ccgataggcc ctcttttgct gaaacacacc aagcttttga aaccatgttc catgactcca
     4261 gcatttctga agaggtagct gaggagcttg ggagagccgc ctcctcgtca tctgttgttc
     4321 catacctgcc ccggctacct atacttcctt ccaagactcg gacactgaag aaacaggtgg
     4381 agaacaagga gaacattgaa ggggcacaag atgccacaga aaattctgct tccagtttag
     4441 caccagggtt catcagaggt gcacaggcct ctagtggatc cccagcactg cctcgaaagc
     4501 aaagagacaa gtcacccagc agcctcttgg aagatgccaa agagacatgc ttcaccaggg
     4561 ataggaaggg gggcttcttc agctccttca tgaagaagag aaatgctccc acacccccca
     4621 aacgcagcag ctccttccga gaaatggaga atcagcccca taagaaatac gaactcacgg
     4681 gtaacttctc atctgttgct tctctacagc atgctgatgg gttctctttc actcctgccc
     4741 agcaagaggc gaatctggtg ccacccaagt gctatggggg gagctttgca cagaggaacc
     4801 tctgtaatga cgacggtggt gggggtgggg gcagtggcac tgctgggggt gggtggtctg
     4861 gcatcacagg cttctttaca ccacgcttaa tcaaaaagac actgggctta cgagcaggta
     4921 aacccacagc cagtgatgac acttccaagc cttttccaag gtcaaactct acatcttcca
     4981 tgtcctcagg gcttccagag caggatagga tggcaatgac ccttcccagg aactgccaga
     5041 ggtccaaact ccagctggaa aggacagtgt ccacctcttc tcagccagaa gagaatgtgg
     5101 acagggccaa tgacatgctt ccaaaaaaat cagaggaaag tgctgctcca agcagggaga
     5161 gaccaaaagc caagttattg cccagaggag ccacagctct tcctctcaga acaccctctg
     5221 gggatctagc cattacagag aaggaccctc caggggtggg agtggctgga gtggcagctg
     5281 cccccaaggg taaagagaag aatggtgggg cacgacttgg gatggctgga gttccagagg
     5341 atggagagca gccgggctgg ccttctccag ccaaggctgc ccccgtcctc ccaaccactc
     5401 acaaccacaa agtgccagtc cttatctcac ccactctgaa acacactcca gctgacgtgc
     5461 agctcattgg cacagactct caggggaata aattcaagct cttatctgag catcaggtca
     5521 catcctctgg agacaaggac cgaccccgac gggtaaaacc aaagtgtgcc ccacccccac
     5581 caccagtgat gagactactg cagcatccgt ccatctgctc agaccctaca gaagagccaa
     5641 ctgccctaac tgcaggacag tccacatcag aaacacagga aggaggaaag aaggcagctc
     5701 tgggcgcagt gcccatcagt gggaaagctg ggaggccagt gatgcctcca cctcaagtgc
     5761 ctctgcccac atcttccatc tcgccagcca aaatggccaa tggcacagca ggtactaaag
     5821 tggctctgag aaaaaccaaa caggccgctg agaaaatctc agcagacaaa atcagcaaag
     5881 aggccctgct ggaatgtgct gacctactgt ccagtgcact cacggaacct gtgcccaaca
     5941 gccagctggt agacactgga caccagctgc ttgactactg ctcaggctat gtggactgca
     6001 tccctcaaac tcgcaacaaa tttgccttcc gagaggctgt gagcaaactg gaactcagcc
     6061 tgcaggagct acaggtttct tcagcagctg ctggtgtgcc cgggacaaac cctgtcctta
     6121 ataacttatt gtcatgtgta caggaaatca gtgatgtggt gcagaggtag acacccagct
     6181 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg
     6241 cggttcgaat acccatacga cgtcccagac tacgcttagt aatgattaat taaactagaa
     6301 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagtaaccc acccaagatc
     6361 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
     6421 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
     6481 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
     6541 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
     6601 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
     6661 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
     6721 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc
     6781 tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg
     6841 ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc
     6901 gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg
     6961 aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct
     7021 tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc
     7081 tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag
     7141 ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt
     7201 cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt
     7261 gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa
     7321 tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc
     7381 ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg
     7441 gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc
     7501 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
     7561 ttttttgtta gacgaagctt ggtaccgagc tcggatctcc accccgtacc ggtcctgcag
     7621 tcgaattcac catgtctaga ctggacaaga gcaaagtcat aaacggagct ctggaattac
     7681 tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     7741 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
     7801 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     7861 actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg
     7921 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     7981 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     8041 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
     8101 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     8161 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     8221 tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact
     8281 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     8341 ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat ccgcggccgc
     8401 actagaggaa ttccgcccct ctcccccccc cccctctccc tccccccccc ctaacgttac
     8461 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     8521 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     8581 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     8641 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     8701 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     8761 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     8821 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     8881 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     8941 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     9001 ataagcttac cggtccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     9061 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     9121 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     9181 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     9241 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     9301 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     9361 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     9421 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     9481 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     9541 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     9601 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     9661 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     9721 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     9781 cgccttctat cgccttcttg acgagttctt ctgatgtaca agtaaagcgg ccgcgactct
     9841 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     9901 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa
     9961 ttcgatatca agcttatcgc gatagatcct aatcaacctc tggattacaa aatttgtgaa
    10021 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
    10081 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
    10141 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
    10201 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
    10261 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    10321 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    10381 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
    10441 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    10501 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    10561 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag
    10621 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
    10681 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10741 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10801 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10861 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
    10921 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg
    10981 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
    11041 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
    11101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    11161 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    11221 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    11281 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    11341 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    11401 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
    11461 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    11521 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    11581 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    11641 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    11701 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    11761 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    11821 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    11881 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    11941 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    12001 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    12061 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    12121 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    12181 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    12241 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    12301 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    12361 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    12421 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    12481 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    12541 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    12601 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    12661 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    12721 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    12781 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    12841 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    12901 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    12961 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    13021 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta
    13081 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    13141 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg
    13201 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    13261 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    13321 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg
    13381 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    13441 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    13501 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    13561 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
