LOCUS       sequence-485266-        7944 bp    DNA     circular SYN 03-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-hNPPC-RFP
FEATURES             Location/Qualifiers
     source          1..7944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          2892..3367
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      3443..3452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3449..4126
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(3579..3597)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3847..3866
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4041..4064
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(4156..4174)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4157..4174)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4194..4211)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4200..4311
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4455..4584
                     /label=AAV2 ITR
     rep_origin      4955..5468
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5042..5061)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5252..5273
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5950..5969)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6069..6091
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6129..6147)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6215..6319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6320..6388,6389..7180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6538..6557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7351..7939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7840..7859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
       61 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      121 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgagcgcg cagagaggga
      181 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      241 tctacgtagc catgctctag aggatccttc gaaatctcca aggcagttat gtgctgatgg
      301 cacacctccc ggtggcccct cctgcccagg cctctgactc ttacccaccc tgcctcccag
      361 gccccagcta agcagaagct gctccacagc ctgccccccg ggaagcctcc ctctgcagat
      421 gaccaccgcc cagtggtcag gagtggcagc tcaccagatt tcccttccaa agatgggaag
      481 gtgagtccag ggaatcggct ctgccacagt aggttggggg tgtcagtttt attaatttag
      541 cctcaaagtt caaacagaga ggagacctag atagtcaatc acctggacgc gaatgtgtct
      601 aggaacacag aagacaggaa ggaccaacct aaggacgttc agacagaagg aagtagccat
      661 gaacagcgga agatttcctt aataaggggc tttagaggat gtactgctgg cccctggaaa
      721 ccagcccagt gcttgactct agagtcagtc tgtgtggctc tgcctgactc tttaaacagg
      781 tataaagaaa gcaagtgtca gagaagttcc agatggtaac atccttcttt gcacagcagg
      841 caaaaagcag agggaacttt cacagcccac ctggataccc ctccctatac tgctttctag
      901 ttcctcttcc tttctcctca aatgaaagag tggtgccact ctatgggtgt tagcacggag
      961 aggaattggg gggctgcgtg gaggtggtga ctcaattcac cagaatccac agaaggtgcc
     1021 ctgttgatgt ctggatcttc ctggagatca ggaaactttg ctgccccaaa ctcagacctc
     1081 aggtgttagc tgtttcctaa gcaaagtgac ctgtgtagtg gttgctccaa tgttgtgagt
     1141 cagggtatga gagtgggaag gggtagcctg agaaaggtgc cgacagagca gaccatgatg
     1201 cggccgcctt agcagctctg ggaccaggct cactaccacg gagggaagag ggaggcttgg
     1261 agggaacctg gttctccaag cccctcttca ctccagttgc cttcctgcct caggcacagc
     1321 ttcctctccc tggaaaatct cgtctgggct actgtctccc cagaatcgcc ttcacgcctg
     1381 gggacagcca ctgcactcct gcggcaacat ttgttctctc tccagcctgc gtcctgctca
     1441 gcagcagcca gtcatagatt cctgctcttc aatcaattcg ggggatctgg gctctccgcc
     1501 tctgcggccc acaccgcagt gcccaaatgc ccaccacact ccttagtgtc caagggaccg
     1561 agaagcccag tgcaaggaga gcaagttcgt gagtggtagg gatatgtggc catgtcccga
     1621 gagccacggg gacctctcta cagtcaaaaa cgggaagttg accacctgtc acggctggca
     1681 gaggctggac tgggccgctg gctaggaagc tctctcccca gcctgtgaaa gtgcacagga
     1741 tactggagcg ggcttccccc agcagcggat cctcgcgtgg gtgcaggcaa ggtcaggtgc
     1801 gcattgttcc cacagaggga gttcaccagc ggagtcagac cccgggacgt tctgtgtggc
     1861 cgaaaccggc tgaacgtgaa cctagagcag taactggcga gcatacgatg ccgcggagca
     1921 cacgcatgag catgaatatc ttggttccgc gctcgcagtc ccctcgttcc cgccttgcgc
     1981 tcaaacactc aggctggcga cacccctgca ctctccagtg tggaagactt gcgctccccc
     2041 ctgcggccca ggcgaacccc gctctccctg cgcctcccac cccagtcctc caaccccatc
     2101 ctctgcctcc ccaacctaac gacgtcgctg tggtctgcgc tcccgccctg accggcaggt
     2161 gaaaggcgga gctgcaatgc catcccgcgg ctgtcaggtg cccagggaag agtttggcga
     2221 caagcagggc tgcgtggatt tcgggcagcg ccaactttct gcctgtatga ctttgggcaa
     2281 gtgacttcat tcctctgagc ctgtctttgc acctgcaaag agggctacgt atcccttcca
     2341 ggtagagtga gtgcgatgag gacctagtgg gtgcacaggg caccaggcgc accgatgctc
     2401 ggcaggccag ggcgcgcgct cgctgcaaat ggagttcccc tgtgcgctca gctctgcagc
     2461 tccaagtgca gcctggagcg agcccgccga gccgcgggat ccctccgggg tgggataagg
     2521 gaggggagcc cccgcggccc cctcccggcc ctcggcgcgg ccgcgtgcgt ggtgtcattg
     2581 gcccgggcgg cccggtgggc gggaggatga catcagcggc aggttggatt ataaaggcgc
     2641 gagcagagtc acgggctcag agcgcaccca gccggcgccg cgcagcactg ggaccctgct
     2701 cgccctgcag cccagccagc ctgctccgca tccccctgct ggtctgcccg ccgacctgcg
     2761 cgccctcgct gccgcccgtg tgcgcccctc gaccccagcg gcaccatgca tctctcccag
     2821 ctgctggcct gcgccctgct gctcacgctg ctctccctgg cgcgccgagc tcggatcctg
     2881 agaacttcag ggtgagtcta tgggaccctt gatgttttct ttccccttct tttctatggt
     2941 taagttcatg tcataggaag gggagaagta acagggtaca catattgacc aaatcagggt
     3001 aattttgcat ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc
     3061 ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat
     3121 gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg caatagcaat
     3181 atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg
     3241 ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg
     3301 gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct
     3361 cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg gcaaagcacg
     3421 tgggccgcct cggccaccgg tcgccaccat ggcctcctcc gaggacgtca tcaaggagtt
     3481 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     3541 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3601 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt
     3661 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt
     3721 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3781 ctccctgcag gacggctcct tcatctacaa ggtgaagttc atcggcgtga acttcccctc
     3841 cgacggcccc gtaatgcaga agaagactat gggctgggag gcctccaccg agcgcctgta
     3901 cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga aggacggcgg
     3961 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg
     4021 ctactactac gtggactcca agctggacat cacctcccac aacgaggact acaccatcgt
     4081 ggagcagtac gagcgcgccg agggccgcca ccacctgttc ctgtagagtt taaaccgtac
     4141 gggaaacggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
     4201 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4261 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4321 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4381 ggaagacaat agcgcggccg ctctagagca tggctacgta gataagtagc atggcgggtt
     4441 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     4501 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     4561 ctcagtgagc gagcgagcgc gcagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4621 ttcccaacag ttgcgcagcc tgaatggcga atggcgattc cgttgcaatg gctggcggta
     4681 atattgttct ggatattacc agcaaggccg atagtttgag ttcttctact caggcaagtg
     4741 atgttattac taatcaaaga agtattgcga caacggttaa tttgcgtgat ggacagactc
     4801 ttttactcgg tggcctcact gattataaaa acacttctca ggattctggc gtaccgttcc
     4861 tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg ctctgattct aacgaggaaa
     4921 gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     4981 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5041 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5101 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5161 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5221 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5281 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5341 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5401 cttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca
     5461 accggggtac atatgattga catgctagtt ttacgattac cgttcatcga ttctcttgtt
     5521 tgctccagac tctcaggcaa tgacctgata gcctttgtag agacctctca aaaatagcta
     5581 ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga
     5641 ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat
     5701 ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg
     5761 caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg
     5821 ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttggaa
     5881 tcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     5941 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     6001 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     6061 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     6121 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     6181 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6241 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6301 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6361 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6421 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6481 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6541 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6601 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6661 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6721 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6781 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6841 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6901 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6961 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7021 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7081 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7141 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7201 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7261 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7921 ggggcggagc ctatggaaaa acgc
//
