LOCUS       sequence-485343-        8363 bp    DNA     circular SYN 04-SEP-2025
DEFINITION  Encodes the G protein to enable transsynaptic RAV infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)
FEATURES             Location/Qualifiers
     source          1..8363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        231..1409
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          462..1400
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1357..1377
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(1440..1473)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1524..1557)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3154..3210)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(3220..3930,3931..3933,3934..3936))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3220..3930,3931..3933,3934..3936))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3262..3283)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3611..3630
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3870..3891
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3933..3942
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3955..4332)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     protein_bind    4348..4381
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4432..4465
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4490..5078
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4543..4563)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4961..4972)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5110..5586
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5309..5328)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5626..5766
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5626..5755
                     /label=AAV2 ITR
     rep_origin      5841..6296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5928..5947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6138..6159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6313..6332)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6432..6454
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6492..6510)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6578..6682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6683..6751,6752..7543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6901..6920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7714..8302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8203..8222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga
      181 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      241 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      301 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      361 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      421 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      481 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      541 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      601 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      661 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      721 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      781 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      841 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      901 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      961 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1021 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1081 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1141 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1201 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1261 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1321 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1381 gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc gactccggaa
     1441 taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga ttgtagctgc
     1501 tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg aagttatggc
     1561 gcgccttact tgtacatctt acagtctgat ctcacctcca ctcttatatg attcccatga
     1621 aggtatgact tttccgcttt gggaagtgac tgacacattc ccccctgtcc ctccaaaact
     1681 gcgttgtttc gattctggtc gattggctct tctgcaccat gtcattaggg aaaatatcaa
     1741 caccaggcca atcatggccc ctgcagtcat caatacatac tttccccagt tcgggagacc
     1801 caggtcaacc cctgagatct gtttgtacac atcggggagg tgaacttcaa caaaatcctc
     1861 agcctcatca ccttctttga aaactgtaga agggtctgcc agggggtgca tcagggggat
     1921 aactgaagat tccaacaact ccatatgttg ctggaggagg gatgattgca tctctgggat
     1981 taggacatgg tcgtcaggcc ctaatattat accattgaaa aacaccccgt tcacatgagg
     2041 atggcacctt cctccaactt tcaaacaccc ttttgagggg atgatctcat tccaggtccg
     2101 gactgacttg tagtgagcat cagcctccat caaggttttg ttgaatatgg tatatgcttt
     2161 tccaaaccct gggacaagtt ttctcaggtg actgagacgt ctgaaactta ctgacttggt
     2221 ggtcatgatg gactctaatg cgtccagaca ttcctctctt ttcttgacta actcctccac
     2281 aacgagatgc tcaatctcgt ctgagcgaaa gtcgtgcaaa ttcaccaact gatctggagg
     2341 gcaccatttg gtctcatctg atgtttgcat cgcgacccat gttccatcca taagtctaag
     2401 tccaagaact ccacataact tgagcctgca tgctcctttt agagacttat acaggcctct
     2461 ttcatccaca aagccgcaag tcttgttccc gttggatgct ctcttccctc tgctattggt
     2521 aaaaatgtca caaggtgtcc ttggtctcgg attctcgggc atccaaatgg tgtaatcatg
     2581 gttagttgag cagtaggtag aggacaccgt tattcctgag cactttccgc cagggaagac
     2641 ccttgagtga agggatttgt catatgggtc caaatctgtc acacttgggg atatgataat
     2701 gagggactct ttggtggttc ttacagttcg aagccagtgg tagtcggggt atggattgtg
     2761 tagggactct tcatatctgg ggtcaccggc catcttccag ttatacgcgg ctctacatgc
     2821 gtctggggtg gggcggaaat gctttctctt gaatgtggtt gtgacataac caacaaagtt
     2881 ggtgtaggtc tctgcctctg tcacaacacc tgtgcaagtg aacccgttca ctttgatggc
     2941 tgagatgtat cccactttga gttccatgta ggagaactcg gacaggttgg tacatccttc
     3001 atcctccaca accaggttat ttggacagct gagatggtgt atgtcaatag ggctccaggg
     3061 accaagttcg tctggtatcg tgtaaatggg gaacttcccg aaacacaacg aaaaacccag
     3121 aaggagtaca aacaaaagaa cctgaggaac cataggtcca gggttctcct ccacgtctcc
     3181 agcctgcttc agcaggctga agttagtagc tccgcttccc ttgtacagct cgtccatgcc
     3241 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
     3301 ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
     3361 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
     3421 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
     3481 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
     3541 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
     3601 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
     3661 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
     3721 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
     3781 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
     3841 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
     3901 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggtg gatccttagc
     3961 gctggtgtac ttggtgatgg ccttagtacc ctcggacacg gcgtgcttgg ccaactcccc
     4021 aggcagcagc aggcgcacgg ccgtctggat ctccctggag gtgatggtcg agcgcttgtt
     4081 gtaatgcgcc aggcgggaag cctcacctgc gatgcgctcg aaaatgtcgt tcacaaacga
     4141 attcatgatg cccatggcct tggacgaaat gccggtgtca gggtggacct gcttcagaac
     4201 cttgtacaca tagatggaat agctctcctt gcggctgcgc ttgcgcttct tgccgccttt
     4261 cttctgcgcc ttagtcaccg ccttcttgga gccctttttc ggggcgggag cagacttcgc
     4321 tggctctggc atggtggcgg ctctagcata acttcgtata aagtatccta tacgaagtta
     4381 tttgccttaa cccagaaatt atcactgtta ttctttagaa tggtgcaaag aataacttcg
     4441 tataatgtat gctatacgaa gttatgaatt cgatatcaag cttatcgata atcaacctct
     4501 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4561 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4621 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4681 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4741 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4801 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4861 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc
     4921 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4981 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     5041 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga
     5101 gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     5161 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     5221 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     5281 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     5341 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     5401 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     5461 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     5521 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     5581 gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga
     5641 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     5701 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     5761 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     5821 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     5941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     6061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     6181 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     6241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     6301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     6361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     6421 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     6481 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     6541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6901 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7201 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7321 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7381 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7441 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7501 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7561 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7621 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7861 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8341 ttgctggcct tttgctcaca tgt
//
