LOCUS       sequence-485460-        9435 bp    DNA     circular SYN 08-SEP-2025
DEFINITION  Virus-induced gene silencing (VIGS) reporter vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVSr
FEATURES             Location/Qualifiers
     source          1..9435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        618..963
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     906..926
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             982..1917
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1972..2148
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     2297..2319
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2311..2328
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2312..2328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2338..2357
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2338..2356
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2381..2397
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2382..2398
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2407..4116
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          3155..4116
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     CDS             4189..5841
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4255..4273)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5903..6079
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6089..6113
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6204..6792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6284..6303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8301..9116)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8301..9116)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9024..9043
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9108..9127
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(9407..9435,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt atcgtacccc tactccaaaa atgtcaaaga tacagtctca
      601 gaagaccaaa gggctattga gacttttcaa caaagggtaa tttcgggaaa cctcctcgga
      661 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      721 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatgcctc tgccgacagt
      781 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
      841 acgtcttcaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa
      901 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      961 acagcccaag ctctagccac catgacttcg aaagtttatg atccagaaca aaggaaacgg
     1021 atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt
     1081 attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac
     1141 gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt
     1201 attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg
     1261 ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag
     1321 atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat
     1381 caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg
     1441 gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa
     1501 atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag
     1561 ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt
     1621 cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt
     1681 gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg
     1741 tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt
     1801 cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
     1861 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc
     1921 tagggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta ttttctccat
     1981 aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg gtttcgctca
     2041 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     2101 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatcga taacattaac
     2161 gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2221 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2281 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     2341 tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat
     2401 aagcttattc gggtcaaggc ggaagccagc gcgccacccc acgtcagcaa atacggaggc
     2461 gcggggttga cggcgtcacc cggtcctaac ggcgaccaac aaaccagcca gaagaaatta
     2521 cagtaaaaaa aaagtaaatt gcactttgat ccacctttta ttacctaagt ctcaatttgg
     2581 atcaccctta aacctatctt ttcaatttgg gccgggttgt ggtttggact accatgaaca
     2641 acttttcgtc atgtctaact tccctttcag caaacatatg aaccatatat agaggagatc
     2701 ggccgtatac tagagctgat gtgtttaagg tcgttgattg cacgagaaaa aaaaatccaa
     2761 atcgcaacaa tagcaaattt atctggttca aagtgaaaag atatgtttaa aggtagtcca
     2821 aagtaaaact tatagataat aaaatgtggt ccaaagcgta attcactcaa aaaaaatcaa
     2881 cgagacgtgt accaaacgga gacaaacggc atcttctcga aatttcccaa ccgctcgctc
     2941 gcccgcctcg tcttcccgga aaccgcggtg gtttcagcgt ggcggattct ccaagcagac
     3001 ggagacgtca cggcacggga ctcctcccac cacccaaccg ccataaatac cagccccctc
     3061 atctcctctc ctcgcatcag ctccaccccc gaaaaatttc tccccaatct cgcgaggctc
     3121 tcgtcgtcga atcgaatcct ctcgcgtcct caaggtacgc tgcttctcct ctcctcgctt
     3181 cgtttcgatt cgatttcgga cgggtgaggt tgttttgttg ctagatccga ttggtggtta
     3241 gggttgtcga tgtgattatc gtgagatgtt taggggttgt agatctgatg gttgtgattt
     3301 gggcacggtt ggttcgatag gtggaatcgt ggttaggttt tgggattgga tgttggttct
     3361 gatgattggg gggaattttt acggttagat gaattgttgg atgattcgat tggggaaatc
     3421 ggtgtagatc tgttggggaa ttgtggaact agtcatgcct gagtgattgg tgcgatttgt
     3481 agcgtgttcc atcttgtagg ccttgttgcg agcatgttca gatctactgt tccgctcttg
     3541 attgagttat tggtgccatg ggttggtgca aacacaggct ttaatatgtt atatctgttt
     3601 tgtgtttgat gtagatctgt agggtagttc ttcttagaca tggttcaatt atgtagcttg
     3661 tgcgtttcga tttgatttca tatgttcaca gattagataa tgatgaactc ttttaattaa
     3721 ttgtcaatgg taaataggaa gtcttgtcgc tatatctgtc ataatgatct catgttacta
     3781 tctgccagta atttatgcta agaactatat tagaatatca tgttacaatc tgtagtaata
     3841 tcatgttaca atctgtagtt catctatata atctattgtg gtaatttctt tttactatct
     3901 gtgtgaagat tattgccact agttcattct acttatttct gaagttcagg atacgtgtgc
     3961 tgttactacc tatctgaata catgtgtgat gtgcctgtta ctatcttttt gaatacatgt
     4021 atgttctgtt ggaatatgtt tgctgtttga tccgttgttg tgtccttaat cttgtgctag
     4081 ttcttaccct atctgtttgg tgattatttc ttgcagatag ttatcggatc catccggggg
     4141 gcaatgagat ccgagaccgt aggtctcatg gtgagcaagg gcgaggagat ggaagacgcc
     4201 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     4261 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     4321 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     4381 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     4441 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     4501 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     4561 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     4621 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     4681 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     4741 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     4801 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     4861 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     4921 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     4981 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     5041 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     5101 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     5161 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     5221 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     5281 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     5341 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     5401 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     5461 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     5521 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     5581 ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     5641 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     5701 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     5761 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     5821 ggcggaaaga tcgccgtgta attctagaga attcggtacg ctgaaatcac cagtctctct
     5881 ctacaaatct atctctctct attttctcca taaataatgt gtgagtagtt tcccgataag
     5941 ggaaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
     6001 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     6061 cagtactaaa atccagatcc actagccttg acaggatata ttggcgggta aactaagtcg
     6121 ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6661 tgcgctctgc tgaagccagt taccttcgga agaagagttg gtagctcttg atccggcaaa
     6721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6901 ttaaattaaa aatgaagttt taaatcaatc taaagtactg agagatcccc tcataatttc
     6961 cccaaagcgt aaccatgtgt gaataaattt tgagctagta gggttgcagc cacgagtaag
     7021 tcttcccttg ttattgtgta gccagaatgc cgcaaaactt ccatgcctaa gcgaactgtt
     7081 gagagtacgt ttcgatttct gactgtgtta gcctggaagt gcttgtccca accttgtttc
     7141 tgagcatgaa cgcccgcaag ccaacatgtt agttgaagca tcagggcgat tagcagcatg
     7201 atatcaaaac gctctgagct gctcgttcgg ctatggcgta ggcctagtcc gtaggcagga
     7261 cttttcaagt ctcggaaggt ttcttcaatc tgcattcgct tcgaatagat attaacaagt
     7321 tgtttgggtg ttcgaatttc aacaggtaag ttagttgcta gaacccatgg ctcctttgcc
     7381 gacgctgagt agattttagg tgacgggtgg tgacaatgag tccgtgtcga gcgctgattt
     7441 tttcggcctt tagagcgaga tttatacaat agaatttggc atgagattgg attgctttta
     7501 gtcagcctct tatagcctaa agtctttgag tgactagatg acatatcatg taagttgctg
     7561 ataggtttcc agttttccgc tcctaggtct gcatattgta cttttcctct tactcgactt
     7621 aaccagtacc aacccagctt ctcaacggat ttataccatg gcactttaaa gccagcatca
     7681 ctgacaatga gcggtgtggt gttactcggt agaatgctcg caaggtcggc tagaaattgg
     7741 tcatgagctt tctttgaaca ttgctctgaa agcgggaacg ctttctcata aagagtaaca
     7801 gaacgaccgt gtagtgcgac tgaagctcgc aataccataa gtcgtttttg ctcacgaata
     7861 tcagaccagt caacaagtac aatgggcatc gtattgcccg aacagataaa gctagcatgc
     7921 caacggtata cagcgagtcg ctctttgtgg aggtgacgat tacctaacaa tcggtcgatt
     7981 cgtttgatgt tatgttttgt tctcgctttg gttggcaggt tacggccaag ttcggtaaga
     8041 gtgagagttt tacagtcaag taatgcgtgg caagccaacg ttaagctgtt gagtcgtttt
     8101 aagtgtaatt cggggcagaa ttggtaaaga gagtcgtgta aaatatcgag ttcgcacatc
     8161 ttgttgtctg attattgatt tttcgcgaaa ccatttgatc atatgacaag atgtgtatcc
     8221 accttaactt aatgattttt accaaaatca ttaggggatt catcagtcta aagtatatat
     8281 gtgtaacatt ggtctagtga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     8341 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     8401 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     8461 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     8521 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     8581 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     8641 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     8701 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     8761 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc tgggatcgca
     8821 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     8881 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     8941 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcggtagatt
     9001 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     9061 atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg gctcataaca
     9121 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     9181 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     9241 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     9301 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     9361 ggctccctca ctttctggct ggatgatggg gcgattcagg cgatccccat ccaacagccc
     9421 gccgtcgagc gggct
//
