LOCUS       sequence-485502-        3952 bp    DNA     circular SYN 08-SEP-2025
DEFINITION  Expresses cat gene under the control of PcadBA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Lee B, Mannone JA, Wang T
  TITLE     A genetically encoded selection for amyloid-beta oligomer binders
  JOURNAL   bioRxiv 2025.03.11.642651; doi: 10.1101/2025.03.11.642651
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.03.11.642651; doi: 10.1101/2025.03.11.642651"
COMMENT     Sequence Label: pBL140
FEATURES             Location/Qualifiers
     source          1..3952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..951)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      999..1221
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2147..2806
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2213..2232)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(join(2881..3672,3673..3741))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQDQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3504..3523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3742..3846)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3914..3932
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctgtaactcc gggttgattt
     1801 atgctcggaa atatttgttg ttgagttttt gtatgttcct gttggtataa tatgttgcgg
     1861 caatttattt gccgcataat ttttattaca taaatttaac cagagaatgt cacgcaatcc
     1921 attgtaaaca ttaaatgttt atcttttcat gatatcaact tgcgatcctg atgtgttaat
     1981 aaaaaacctc aagttctcac ttacagaaac ttttgtgtta tttcacctaa tctttaggat
     2041 taatcctttt ttcgtgagta atcttatcgc cagtttggtc tggtcaggaa atagttatac
     2101 atcatgaccc ggactccaaa ttcaaaaatg aaattaggag aagagcatgg agaaaaaaat
     2161 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     2221 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     2281 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgctcg
     2341 cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc tggtgatatg
     2401 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     2461 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     2521 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     2581 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     2641 cttcttcgcc cccgttttca ctatgggcaa atattatacg caaggcgaca aggtgctgat
     2701 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     2761 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaatta acggcactcc
     2821 tcagcaaata taatgaccct cttgataacc caagagggca ttttttaatg cccatggcgt
     2881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3481 atcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3841 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     3901 attaacctat aaaaataggc gtatcacgag gcccttaggc caaataggcc gt
//
