LOCUS       sequence-485587-        3816 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Bacterial expression of GST-ScAtg38 Delta(123 to 181).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O,
            Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi 
            M, Ktistakis NT, Sachse C, Williams RL
  TITLE     Characterization of Atg38 and NRBF2, a fifth subunit of the 
            autophagic Vps34/PIK3C3 complex.
  JOURNAL   Autophagy. 2016 Nov;12(11):2129-2144. doi: 
            10.1080/15548627.2016.1226736. Epub 2016 Sep 14.
  PUBMED    27630019
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2016.1226736"; journalName: "Autophagy"; date: 
            "2016-11"; volume: "12"; issue: "11"; pages: "2129-2144"
COMMENT     Sequence Label: pYO1091
FEATURES             Location/Qualifiers
     source          1..3816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     128..150
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     203..222
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        203..221
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             295..317
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             328..981
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     939..961
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1000..1020
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(1617..1635)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1631..1678
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1723..1741)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1809..1913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1914..1982,1983..2774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2132..2151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2945..3533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3434..3453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3687..3704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(3719..3816,1..42)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3784..3803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
       61 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      121 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
      181 caggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
      241 tctagtgccg gctccggaga gctccaattg gaattcgcta gctctagaaa taattttgtt
      301 taactttaag aaggagatat acataatatg tcccctatac taggttattg gaaaattaag
      361 ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat
      421 ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag
      481 tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc
      541 atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag
      601 atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat
      661 agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa
      721 atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct
      781 gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat
      841 gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag
      901 tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt
      961 ggtggcgacc atcctccaaa atcggattct tctggttccg aaaacctgta tttccagggc
     1021 tcccatatga gtactttagc ggaagtttat actattattg aggacgccga acaggaatgt
     1081 cgaaaaggag attttacaaa tgcaaaagcg aagtatcaag aagccattga agttttaggt
     1141 ccgcagaacg agaatttgtc tcaaaataaa ttaagttctg acgttacgca agccattgac
     1201 ctattaaaac aggacattac agccaaaatc caagaattgg aacttttgat tgaaaagcag
     1261 tcatcagaag aaaataatat tggaatggtc aacaacaata tgcttattgg ctctgttatt
     1321 ttaaacaaca aatctccgat aaatggaata agcaatgcta ggaattggga taacccagca
     1381 tatcaggaca cttttaaaga atatggtatg aaaattgacg aaattacgaa agaaaataaa
     1441 aaattggcta atgaaattgg gcgattaaga gaacgctggg acagcttagt agaaagtgcg
     1501 aagcaaagaa gggataagca aaaaaactag ggatccagat ctaagcttgg taccacgcgt
     1561 gcgcgctgat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     1621 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     1681 gaaaggagga actatagccg gataattctt gaagacgaaa gggcctcgtg atacgcctat
     1741 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1801 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1861 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1921 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1981 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2041 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2101 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     2161 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2221 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2281 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2341 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2401 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     2461 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2521 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2581 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2641 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2701 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2761 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2821 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2881 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3061 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3721 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct
     3781 cagtacaatc tgctctgatg ccgcatagtt aagcca
//
