LOCUS       sequence-485600-       10791 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Destination vector for pSplit system in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Yuan G, Islam MT, Tuskan GA, Yang X
  TITLE     A Novel Gene Stacking Method in Plant Transformation Utilizing Split
            Selectable Markers.
  JOURNAL   Bio Protoc. 2025 Feb 20;15(4):e5214. doi: 10.21769/BioProtoc.5214. 
            eCollection 2025 Feb 20.
  PUBMED    40028010
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bio 
            Protoc."; date: "2025-02-20"; volume: "15(4):e5214. doi"; pages: " 
            10.21769/BioProtoc.5214. eCollection 2025 Feb 20"
COMMENT     Sequence Label: pSplit 1
FEATURES             Location/Qualifiers
     source          1..10791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        269..614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1400..1648
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(2337..2353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2337..2353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2350..2372)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2361..2377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2385..2391,2392..2409,2410..2415))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2430..2451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2642..3318
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(3425..3444)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    4260..4434
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4512..4536
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4961..5755
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5842..6430
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6331..6350
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6584..6601
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6616..6756
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6682..6701)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6842..6864
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7100..7294
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7360..8433)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8862..9491)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta attcccgctt acgagaccgt
       61 tggtctcctc cactgaatca aaggccatgg agtcaaagat tcaaatagag gacctaacag
      121 aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc aatgacaaga
      181 agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag
      241 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
      301 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
      361 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
      421 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      481 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
      541 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
      601 atttggagag aacacggggg actcaaacat tactcattca caaaaccatc ttaaagcaac
      661 tacacaagtc ttgaaatttt ctcatatttt ctatttacta tataaacttt taatcaaatc
      721 aagattaaag ttaattaaat gacaaccttc aaaatcgagt cccggatcca cggcaacctc
      781 aacggggaga agttcgagtt ggttggaggt ggagtaggtg aggagggtcg cctcgagatt
      841 gagatgaaga ctaaagataa accactggca ttctctccct tcctgctgac cacttgcatg
      901 ggttacgggt tctaccactt cgccagcttc ccaaagggga ttaagaacat ctatcttcat
      961 gctgcaacga acggaggtta caccaacacc aggaaggaga tctatgaaga cggcggcatc
     1021 ttggaggtca acttccgtta cacttacgag ttcaacaaga tcatcggtga cgtcgagtgc
     1081 attggacatg gattcccaag tcagagtccg atcttcaagg acacgatcgt gaagagttgt
     1141 cccacggtgg acctgatgtt gccaatgtcc gggaacatca tcgccagctc ctacgcttac
     1201 gccttccaac tgaaggacgg ctctttctac acggcagaag tcaagaacaa catagacttc
     1261 aagaatccaa tccacgagtc cttctcgaag tcagggccca tgttcaccca cagacgtgtc
     1321 gaggagactc tcaccaagga gaaccttgcc atagtggagt accagcaggt tttcaacagc
     1381 gccccaagag acatgtagat atgaagatga agatgaaata tttggtgtgt caaataaaaa
     1441 gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat ttgtggcttt
     1501 ttctaatatt aaatgaatgt aagatctcat tataatgaat aaacaaatgt ttctataatc
     1561 cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg tgctatggta
     1621 tggactatgg aatatgatta aagataagat gggctcatag agtaaaacga ggcgagggac
     1681 ctataaacct cccttcatca tgctatttca tgatctattt tataaaataa agatgtagaa
     1741 aaaagtaagc gtaataaccg caaaacaaat gatttaaaac atggcacata atgaggagat
     1801 taagttcggt ttacgtttat tttagtacta attgtaacgt gagactacgt atcgggaatc
     1861 gcctaattaa agcattaatg cgaacctgat tagattcacc gaccctccta tcgtgtcgac
     1921 ctttctgttt cttagaattt tttggtagtc tatgtactaa taatgtcagc ttcgtattta
     1981 tttcataagc aatttgcatt tgcaatttgt tttttacttt tatttttatt gtattgtgga
     2041 atgtggactc gtaccaacat gaagttatat accaccaaaa aaattacagt tagtcaaaag
     2101 attcacgagt gagagctact tatgattgtc ttttacgtat atgtctaatt gtctatttgc
     2161 tcaataatct ttgtactttc ttttgtcgtt gataaaatca caaagttcca aaagtaatcg
     2221 aatgatttgc ttttaagaaa agaagagctc aataattcaa catatatctg tacacaatta
     2281 ccgcctttga gtgagctcta gatgccacct gacgtctaag aagaattcgt aatcatgtca
     2341 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2401 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2461 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2521 caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca gcttgccaac atggtggagc
     2581 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta
     2641 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
     2701 tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt
     2761 gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac
     2821 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     2881 tggattgatg tgaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat
     2941 acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac
     3001 ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa
     3061 ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct
     3121 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac
     3181 gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
     3241 gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
     3301 ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct ctcgagcttt
     3361 cgcagatatg gggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     3421 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     3481 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     3541 gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     3601 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     3661 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     3721 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     3781 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     3841 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     3901 catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgt cctacgaaac
     3961 cgagattctt actgttgagt atggattgct tccaatcggc aagatcgtag agaaacgtat
     4021 tgagtgtaca gtttattctg ttgacaacaa cggaaatatc tatactcagc ctgttgctca
     4081 atggcacgat agaggggaac aagaggtttt cgagtattgc cttgaggatg gtagcctcat
     4141 tcgagcaaca aaagatcaca aatttatgac agtggatggt cagatgttac ctatcgatga
     4201 aattttcgaa agggaattag acctcatgag agtggataac ctccctaatt aggctcgagt
     4261 ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt
     4321 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     4381 tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcccccga
     4441 attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     4501 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     4561 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     4621 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     4681 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     4741 tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg
     4801 atactatgtt atacgccaat tttgaaaaca actttgaaaa agctgttttc tggtatttaa
     4861 ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg
     4921 gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc
     4981 accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc
     5041 tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga
     5101 cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct
     5161 ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa
     5221 tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag
     5281 ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat
     5341 atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg attacttact
     5401 gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga
     5461 tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc
     5521 ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat
     5581 tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc
     5641 gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat
     5701 caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct
     5761 agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5821 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5881 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5941 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6001 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6061 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6361 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6421 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6481 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6541 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6601 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6661 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     6721 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     6781 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6841 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
     6901 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
     6961 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
     7021 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
     7081 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
     7141 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
     7201 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
     7261 cggctttggg ttcccaatgt acgtgctatc cacaggaaac tctccttttc gacctttttc
     7321 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
     7381 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
     7441 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
     7501 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
     7561 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
     7621 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
     7681 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
     7741 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
     7801 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
     7861 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
     7921 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
     7981 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
     8041 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
     8101 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
     8161 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
     8221 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
     8281 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
     8341 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
     8401 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
     8461 gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct
     8521 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
     8581 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
     8641 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
     8701 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
     8761 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
     8821 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
     8881 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
     8941 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
     9001 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
     9061 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
     9121 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
     9181 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
     9241 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
     9301 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
     9361 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
     9421 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
     9481 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
     9541 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
     9601 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
     9661 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
     9721 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
     9781 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
     9841 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
     9901 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
     9961 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
    10021 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
    10081 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
    10141 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
    10201 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
    10261 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
    10321 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
    10381 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
    10441 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
    10501 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
    10561 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
    10621 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg
    10681 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
    10741 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta g
//
