LOCUS       sequence-485608-       10685 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  TMP-inducible strong RNA regulator (tethered to MCP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Thurm AR, Finkel Y, Andrews C, Cai XS, Benko C, Bintu L
  TITLE     High-throughput discovery of regulatory effector domains in human 
            RNA-binding proteins.
  JOURNAL   bioRxiv [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317.
  PUBMED    39071298
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317."
COMMENT     Sequence Label: synNANOS
FEATURES             Location/Qualifiers
     source          1..10685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    100..225
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    722..955
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1140..1184
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1482..1599
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1722..1933
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1934..2872
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2829..2849
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4107..4997
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             5268..5321
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6048..6104
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6105..6503
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(6523..6556)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6612..7200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6665..6685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7083..7094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7203..7219)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7204..7220)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7729..7909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7971..8559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8051..8070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8730..9521,9522..9590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9353..9372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9591..9695)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9770..9789)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9961..10340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10342..10540
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10495..10515
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             join(10558..10685,1..53)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
       61 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      121 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      181 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      241 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      301 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      361 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      421 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      481 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      541 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
      601 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
      661 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
      721 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
      781 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
      841 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
      901 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
      961 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1021 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1081 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1141 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1201 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1261 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     1321 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     1381 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     1441 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     1501 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     1561 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     1621 agagatccag tttggttagt accgggcccg ctctagagat cccatttgac tcaggcggcc
     1681 gcgtggataa ccgtattacc gccatgcatg tgcccgtcag tgggcagagc gcacatcgcc
     1741 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     1801 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     1861 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     1921 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     1981 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
     2041 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     2101 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     2161 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     2221 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     2281 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     2341 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     2401 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     2461 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     2521 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     2581 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     2641 ggcgccgtcc aggcacctcg attagttctc gcgcttttgg agtacgtcgt ctttaggttg
     2701 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     2761 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     2821 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     2881 agctagccac catgatctca cttattgctg cactcgcagt cgactacgtt atcgggatgg
     2941 agaacgctat gccatggaat ctgcccgccg acctcgcgtg gttcaagcgc aacacattga
     3001 acaagcccgt tatcatgggc cgacacactt gggaatccat tggcagaccg ctccctggta
     3061 gaaaaaatat catactctcc tcacagccct ctactgatga tagagttaca tgggtgaaat
     3121 cagtagatga ggcgattgcg gcttgtggcg atgtcccgga aattatggtt atagggggtg
     3181 ggcgggtaat tgagcaattt ctccctaagg cgcagaagct ctacctgacg cacatcgacg
     3241 ctgaggtaga gggggacacg cacttccctg actacgaacc tgacgactgg gagtccgtgt
     3301 tttctgaatt ccacgacgcg gacgcacaga attcccattc ctactgtttt gaaatacttg
     3361 aacggctagc cgttaaaatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg
     3421 gaactggcga cgtgactgtc gccccaagca acttcgctaa cgggatcgct gaatggatca
     3481 gctctaactc gcgttcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc
     3541 agaatcgcaa atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt tcgtacttaa
     3601 atatggaact aaccattcca attttcgcca cgaattccga ctgcgagctt attgttaagg
     3661 caatgcaagg tctcctaaaa gatggaaacc cgattccctc agcaatcgca gcaaactccg
     3721 gcatctacgc gatctacgcc atggcttcta tggcttctaa ctttactcag ttcgttctcg
     3781 tcgacaatgg cggaactggc gacgtgactg tcgccccaag caacttcgct aacgggatcg
     3841 ctgaatggat cagctctaac tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc
     3901 agagctctgc gcagaatcgc aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc
     3961 gttcgtactt aaatatggaa ctaaccattc caattttcgc cacgaattcc gactgcgagc
     4021 ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg
     4081 cagcaaactc cggcatctac gcgatcatgg cagaaatcgg tactggcttt ccattcgacc
     4141 cccattatgt ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt ccgcgcgatg
     4201 gcacccctgt gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg cgcaacatca
     4261 tcccgcatgt tgcaccgacc catcgctgca ttgctccaga cctgatcggt atgggcaaat
     4321 ccgacaaacc agacctgggt tatttcttcg acgaccacgt ccgcttcatg gatgccttca
     4381 tcgaagccct gggtctggaa gaggtcgtcc tggtcattca cgactggggc tccgctctgg
     4441 gtttccactg ggccaagcgc aatccagagc gcgtcaaagg tattgcattt atggagttca
     4501 tccgccctat cccgacctgg gacgaatggc cagaatttgc ccgcgagacc ttccaggcct
     4561 tccgcaccac cgacgtcggc cgcaagctga tcatcgatca gaacgttttt atcgagggta
     4621 cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat taccgcgagc
     4681 cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt cccaaacgag ctgccaatcg
     4741 ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata catggactgg ctgcaccagt
     4801 cccctgtccc gaagctgctg ttctggggca ccccaggcgt tctgatccca ccggccgaag
     4861 ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc ccgggtctga
     4921 atctgctgca agaagacaac ccggacctga tcggcagcga gatcgcgcgc tggctgtcga
     4981 cgctcgagat ttccggcggt ggatccggat caggctccgc tctggtgcct agtgcaagat
     5041 acgtgtctgc ccctggcccc gctcatcccc agccattctc cagctggaac gattatctcg
     5101 ggctggcgac cctgatcaca aaggccgtcg acggcgaacc aaggtttggc tgcgcacggg
     5161 gcggcaatgg aggtggggga tcacccccat cctcttccag cagcagttgt tgctcacctc
     5221 acactggggc tggtcccggg gccttgggac cggcccttgg atctggagag ggaaggggaa
     5281 gcctcctaac ttgcggagat gtcgaggaga atcctggccc aatggttagt aagggagagg
     5341 agttgataaa agagaatatg cacatgaaac tgtatatgga agggacagtg gacaaccatc
     5401 acttcaagtg cacctctgag ggagaaggaa agccatacga aggcacccag actatgcgaa
     5461 ttaaggtcgt ggagggaggg cctctcccct tcgccttcga tattcttgca acatctttcc
     5521 tctacgggag caaaaccttc atcaatcaca cacaaggcat acctgacttt tttaagcaga
     5581 gttttccaga gggctttacc tgggagcgtg tgactacata cgaagatggg ggtgtgctga
     5641 cggctacaca ggatacttca cttcaggacg gttgtctgat ctacaacgtc aagatcaggg
     5701 gggtcaattt cacctcgaat ggaccagtaa tgcaaaaaaa gaccctggga tgggaagctt
     5761 ttactgaaac tctctatccc gccgacggcg gcctcgaagg cagaaacgat atggctttga
     5821 agctggttgg cgggtcccat ctaattgcca atgccaaaac cacatatagg agcaaaaagc
     5881 ctgcaaagaa cctgaagatg ccgggtgtgt actatgttga ctatagatta gagcggatca
     5941 aagaggcaaa taacgaaacg tacgtggagc agcacgaggt agccgtggcg cgctactgcg
     6001 atttaccctc caaactgggc cataaactga actgtacagg aagcggagct actaacttca
     6061 gcctgctgaa gcaggctggc gacgtggaag aaaacccagg acctatggcc aagcctttgt
     6121 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     6181 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     6241 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     6301 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
     6361 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
     6421 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
     6481 ctggttatgt gtgggagggc taagaattcg tcgagggacc taataacttc gtatagcata
     6541 cattatacga agttatacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca
     6601 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6661 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6721 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6781 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6841 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6901 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6961 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7021 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7081 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7141 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7201 atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     7261 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     7321 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7381 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7441 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     7501 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
     7561 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
     7621 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
     7681 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     7741 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7801 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7861 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catgtgagca
     7921 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7981 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8041 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8101 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8161 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8221 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8281 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8341 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8401 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8461 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8521 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8581 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8641 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8701 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8761 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8821 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8881 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8941 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9001 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9061 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9121 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9181 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9241 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9301 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9361 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9421 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9481 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9541 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9601 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9661 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9721 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     9781 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     9841 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     9901 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     9961 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
    10021 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
    10081 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
    10141 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
    10201 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
    10261 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
    10321 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
    10381 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
    10441 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
    10501 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
    10561 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    10621 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    10681 gactc
//
