LOCUS       sequence-485610-       10466 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Degron-tagged MCP construct for generating inducibly stable tethered
            RBPs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Thurm AR, Finkel Y, Andrews C, Cai XS, Benko C, Bintu L
  TITLE     High-throughput discovery of regulatory effector domains in human 
            RNA-binding proteins.
  JOURNAL   bioRxiv [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317.
  PUBMED    39071298
REFERENCE   2  (bases 1 to 10466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317."
COMMENT     Sequence Label: pAT124
FEATURES             Location/Qualifiers
     source          1..10466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    100..225
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    722..955
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1140..1184
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1482..1599
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1722..1933
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1934..2872
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2829..2849
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4107..4997
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             5049..5102
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5829..5885
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5886..6284
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(6304..6337)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6393..6981
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6446..6466)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6864..6875)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6984..7000)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6985..7001)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7510..7690
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7752..8340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7832..7851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8511..9302,9303..9371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9134..9153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9372..9476)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9551..9570)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9742..10121
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10123..10321
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10276..10296
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             join(10339..10466,1..53)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
       61 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      121 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      181 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      241 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      301 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      361 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      421 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      481 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      541 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
      601 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
      661 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
      721 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
      781 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
      841 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
      901 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
      961 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1021 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1081 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1141 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1201 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1261 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     1321 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     1381 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     1441 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     1501 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     1561 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     1621 agagatccag tttggttagt accgggcccg ctctagagat cccatttgac tcaggcggcc
     1681 gcgtggataa ccgtattacc gccatgcatg tgcccgtcag tgggcagagc gcacatcgcc
     1741 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     1801 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     1861 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     1921 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     1981 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
     2041 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     2101 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     2161 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     2221 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     2281 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     2341 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     2401 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     2461 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     2521 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     2581 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     2641 ggcgccgtcc aggcacctcg attagttctc gcgcttttgg agtacgtcgt ctttaggttg
     2701 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     2761 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     2821 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     2881 agctagccac catgatctca cttattgctg cactcgcagt cgactacgtt atcgggatgg
     2941 agaacgctat gccatggaat ctgcccgccg acctcgcgtg gttcaagcgc aacacattga
     3001 acaagcccgt tatcatgggc cgacacactt gggaatccat tggcagaccg ctccctggta
     3061 gaaaaaatat catactctcc tcacagccct ctactgatga tagagttaca tgggtgaaat
     3121 cagtagatga ggcgattgcg gcttgtggcg atgtcccgga aattatggtt atagggggtg
     3181 ggcgggtaat tgagcaattt ctccctaagg cgcagaagct ctacctgacg cacatcgacg
     3241 ctgaggtaga gggggacacg cacttccctg actacgaacc tgacgactgg gagtccgtgt
     3301 tttctgaatt ccacgacgcg gacgcacaga attcccattc ctactgtttt gaaatacttg
     3361 aacggctagc cgttaaaatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg
     3421 gaactggcga cgtgactgtc gccccaagca acttcgctaa cgggatcgct gaatggatca
     3481 gctctaactc gcgttcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc
     3541 agaatcgcaa atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt tcgtacttaa
     3601 atatggaact aaccattcca attttcgcca cgaattccga ctgcgagctt attgttaagg
     3661 caatgcaagg tctcctaaaa gatggaaacc cgattccctc agcaatcgca gcaaactccg
     3721 gcatctacgc gatctacgcc atggcttcta tggcttctaa ctttactcag ttcgttctcg
     3781 tcgacaatgg cggaactggc gacgtgactg tcgccccaag caacttcgct aacgggatcg
     3841 ctgaatggat cagctctaac tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc
     3901 agagctctgc gcagaatcgc aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc
     3961 gttcgtactt aaatatggaa ctaaccattc caattttcgc cacgaattcc gactgcgagc
     4021 ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg
     4081 cagcaaactc cggcatctac gcgatcatgg cagaaatcgg tactggcttt ccattcgacc
     4141 cccattatgt ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt ccgcgcgatg
     4201 gcacccctgt gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg cgcaacatca
     4261 tcccgcatgt tgcaccgacc catcgctgca ttgctccaga cctgatcggt atgggcaaat
     4321 ccgacaaacc agacctgggt tatttcttcg acgaccacgt ccgcttcatg gatgccttca
     4381 tcgaagccct gggtctggaa gaggtcgtcc tggtcattca cgactggggc tccgctctgg
     4441 gtttccactg ggccaagcgc aatccagagc gcgtcaaagg tattgcattt atggagttca
     4501 tccgccctat cccgacctgg gacgaatggc cagaatttgc ccgcgagacc ttccaggcct
     4561 tccgcaccac cgacgtcggc cgcaagctga tcatcgatca gaacgttttt atcgagggta
     4621 cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga gatggaccat taccgcgagc
     4681 cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt cccaaacgag ctgccaatcg
     4741 ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata catggactgg ctgcaccagt
     4801 cccctgtccc gaagctgctg ttctggggca ccccaggcgt tctgatccca ccggccgaag
     4861 ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt ggacatcggc ccgggtctga
     4921 atctgctgca agaagacaac ccggacctga tcggcagcga gatcgcgcgc tggctgtcga
     4981 cgctcgagat ttccggcggt ggatccggat caggctccgg agacgggtca agcgtctctg
     5041 gatctggaga gggaagggga agcctcctaa cttgcggaga tgtcgaggag aatcctggcc
     5101 caatggttag taagggagag gagttgataa aagagaatat gcacatgaaa ctgtatatgg
     5161 aagggacagt ggacaaccat cacttcaagt gcacctctga gggagaagga aagccatacg
     5221 aaggcaccca gactatgcga attaaggtcg tggagggagg gcctctcccc ttcgccttcg
     5281 atattcttgc aacatctttc ctctacggga gcaaaacctt catcaatcac acacaaggca
     5341 tacctgactt ttttaagcag agttttccag agggctttac ctgggagcgt gtgactacat
     5401 acgaagatgg gggtgtgctg acggctacac aggatacttc acttcaggac ggttgtctga
     5461 tctacaacgt caagatcagg ggggtcaatt tcacctcgaa tggaccagta atgcaaaaaa
     5521 agaccctggg atgggaagct tttactgaaa ctctctatcc cgccgacggc ggcctcgaag
     5581 gcagaaacga tatggctttg aagctggttg gcgggtccca tctaattgcc aatgccaaaa
     5641 ccacatatag gagcaaaaag cctgcaaaga acctgaagat gccgggtgtg tactatgttg
     5701 actatagatt agagcggatc aaagaggcaa ataacgaaac gtacgtggag cagcacgagg
     5761 tagccgtggc gcgctactgc gatttaccct ccaaactggg ccataaactg aactgtacag
     5821 gaagcggagc tactaacttc agcctgctga agcaggctgg cgacgtggaa gaaaacccag
     5881 gacctatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     5941 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     6001 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     6061 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     6121 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     6181 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     6241 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagaattc gtcgagggac
     6301 ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg ttccggttcc
     6361 actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     6421 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6481 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6541 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6601 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6661 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6721 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6781 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6841 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6901 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6961 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     7021 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7081 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     7141 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7201 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7261 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     7321 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     7381 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     7441 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     7501 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7561 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7621 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7681 atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7741 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7801 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7861 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7921 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7981 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8041 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8101 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8161 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8221 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8281 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8341 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8401 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8461 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8521 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8581 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8641 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8701 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8761 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8821 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8881 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8941 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9001 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9061 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9121 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9181 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9241 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9301 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9361 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9421 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9481 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     9541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     9601 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     9661 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     9721 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     9781 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     9841 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     9901 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     9961 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
    10021 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
    10081 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
    10141 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
    10201 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
    10261 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
    10321 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    10381 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    10441 gtgtgtgccc gtctgttgtg tgactc
//
