LOCUS       sequence-485615-        9472 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Expression of human ARID1A ∆IDR1 and ∆IDR2 (deletion of a.a. 2-970 
            and a.a. 1170-1610) using piggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, 
            Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, 
            Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, 
            Cermakova K, Hodges HC
  TITLE     beta-catenin functions as a molecular adapter for disordered cBAF 
            interactions.
  JOURNAL   Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026.
  PUBMED    40695292
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026."
COMMENT     Sequence Label: pPB-CAG-ARID1A-deltaIDR1IDR2-FLAG
FEATURES             Location/Qualifiers
     source          1..9472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     168..187
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        168..186
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   216..250
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        571..950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        952..1227
                     /label=chicken beta-actin promoter
     primer_bind     2168..2190
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      2281..2290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4918..4983
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5078..5627
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5222..5239)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5449..5468
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6098..6115)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6104..6328
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6375..6391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6399..6415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6423..6429,6430..6447,6448..6453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6468..6489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6723..6785
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6826..6846)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6826..6844)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6865..6881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6889..6905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6913..6919,6920..6937,6938..6943))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6958..6979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7096..7113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7267..7855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7347..7366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8026..8817,8818..8886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8649..8668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8887..8991)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9017..9472)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9154..9175)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9366..9385
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgctagac
      541 tcgagaagct tgatcggcca cgcggatctc gacattgatt attgactagt tattaatagt
      601 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      661 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      721 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      781 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      841 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      901 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
      961 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     1021 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca
     1081 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
     1141 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
     1201 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
     1261 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     1321 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     1381 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     1441 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     1501 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     1561 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     1621 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     1681 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     1741 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     1801 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     1861 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     1921 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     1981 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     2041 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     2101 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     2161 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gccggagcgc acgtcggcag
     2221 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca ccgggctagc
     2281 gccaccatgg gggatgtaaa gttaactcca gccaccaaaa tgaacaacaa ggcagatggg
     2341 acacccaaga cagaatccaa atccaagaaa tccagttctt ctactacaac caatgagaag
     2401 atcaccaagt tgtatgagct gggtggtgag cctgagagga agatgtgggt ggaccgttat
     2461 ctggccttca ctgaggagaa ggccatgggc atgacaaatc tgcctgctgt gggtaggaaa
     2521 cctctggacc tctatcgcct ctatgtgtct gtgaaggaga ttggtggatt gactcaggtc
     2581 aacaagaaca aaaaatggcg ggaacttgca accaacctca atgtgggcac atcaagcagt
     2641 gctgccagct ccttgaaaaa gcagtatatc cagtgtctct atgcctttga atgcaagatt
     2701 gaacggggag aagaccctcc cccagacatc tttgcagctg ctgattccaa gaagtcccag
     2761 cccaagatcc agcctccctc tcctgcggga tcaggatcta tgcaggggcc ccagactccc
     2821 cagtcaacca gcagttccat ggcagaagga ggagacttaa agccaccaac tccagcatcc
     2881 acaccaatga aaatgcagaa ggcaggtccc ccagtacctg cctcgcacat agcacctgcc
     2941 cctgtgcagc cccccatgat tcggcgggat atcaccttcc cacctggctc tgttgaagcc
     3001 acacagcctg tgttgaagca gaggaggcgg ctcacaatga aagacattgg aaccccggag
     3061 gcatggcggg taatgatgtc cctcaagtct ggtctcctgg cagagagcac atgggcatta
     3121 gataccatca acatcctgct gtatgatgac aacagcatca tgaccttcaa cctcagtcag
     3181 ctcccagggt tgctagagct ccttgtagaa tatttccgac gatgcctgat tgagatcttt
     3241 ggcattttaa aggagtatga ggtgggtgac ccaggacaga gaacgctact ggatcctggg
     3301 aggttcagca aggtgtctag tccagctccc atggagggtg gggaagaaga agaagaactt
     3361 ctaggtccta aactagaaga ggaagaagaa gaggaagtag ttgaaaatga tgaggagata
     3421 gccttttcag gcaaggacaa gccagcttca gagaatagtg aggagaagct gatcagtaag
     3481 tttgacaagc ttccagtaaa gatcgtacag aagaatgatc catttgtggt ggactgctca
     3541 gataagcttg ggcgtgtgca ggagtttgac agtggcctgc tgcactggcg gattggtggg
     3601 ggggacacca ctgagcatat ccagacccac ttcgagagca agacagagct gctgccttcc
     3661 cggcctcacg caccctgccc accagcccct cggaagcatg tgacaacagc agagggtaca
     3721 ccagggacaa cagaccagga ggggccccca cctgatggac ctccagaaaa acggatcaca
     3781 gccactatgg atgacatgtt gtctactcgg tctagcacct tgaccgagga tggagctaag
     3841 agttcagagg ccatcaagga gagcagcaag tttccatttg gcattagccc agcacagagc
     3901 caccggaaca tcaagatcct agaggacgaa ccccacagta aggatgagac cccactgtgt
     3961 acccttctgg actggcagga ttctcttgcc aagcgctgcg tctgtgtgtc caataccatt
     4021 cgaagcctgt catttgtgcc aggcaatgac tttgagatgt ccaaacaccc agggctgctg
     4081 ctcatcctgg gcaagctgat cctgctgcac cacaagcacc cagaacggaa gcaggcacca
     4141 ctaacttatg aaaaggagga ggaacaggac caaggggtga gctgcaacaa agtggagtgg
     4201 tggtgggact gcttggagat gctccgggaa aacaccttgg ttacactcgc caacatctcg
     4261 gggcagttgg acctatctcc ataccccgag agcatttgcc tgcctgtcct ggacggactc
     4321 ctacactggg cagtttgccc ttcagctgaa gcccaggacc ccttttccac cctgggcccc
     4381 aatgccgtcc tttccccgca gagactggtc ttggaaaccc tcagcaaact cagcatccag
     4441 gacaacaatg tggacctgat tctggccaca ccccccttca gccgcctgga gaagttgtat
     4501 agcactatgg tgcgcttcct cagtgaccga aagaacccgg tgtgccggga gatggctgtg
     4561 gtactgctgg ccaacctggc tcagggggac agcctggcag ctcgtgccat tgcagtgcag
     4621 aagggcagta tcggcaacct cctgggcttc ctagaggaca gccttgccgc cacacagttc
     4681 cagcagagcc aggccagcct cctccacatg cagaacccac cctttgagcc aactagtgtg
     4741 gacatgatgc ggcgggctgc ccgcgcgctg cttgccttgg ccaaggtgga cgagaaccac
     4801 tcagagttta ctctgtacga atcacggctg ttggacatct cggtatcacc gctgatgaat
     4861 agcctcgtgt cacaagtcat ttgtgatgta ctgtttttga ttggccagtc caccggtgac
     4921 tacaaagacc atgacggtga ttataaagat catgatatcg attacaagga tgacgatgac
     4981 aagtgaacgc gttagtgcgg atcctagtgc gaattctagt gcgtcgactc tagcaacggt
     5041 ttccctctag cgggatcaat tccgcccccc ccccctaacg ttactggccg aagccgcttg
     5101 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     5161 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5221 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5281 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     5341 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     5401 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     5461 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     5521 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     5581 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tggtcatgaa
     5641 aacatttaac atttctcaac aagatctaga attagtagaa gtagcgacag agaagattac
     5701 aatgctttat gaggataata aacatcatgt gggagcggca attcgtacga aaacaggaga
     5761 aatcatttcg gcagtacata ttgaagcgta tataggacga gtaactgttt gtgcagaagc
     5821 cattgcgatt ggtagtgcag tttcgaatgg acaaaaggat tttgacacga ttgtagctgt
     5881 tagacaccct tattctgacg aagtagatag aagtattcga gtggtaagtc cttgtggtat
     5941 gtgtagggag ttgatttcag actatgcacc agattgtttt gtgttaatag aaatgaatgg
     6001 caagttagtc aaaactacga ttgaagaact cattccactc aaatataccc gaaattaaaa
     6061 gtcgagatgc atcgatgatc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     6121 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6181 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6241 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6301 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaacctg cagcccaagc
     6361 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6421 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6481 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6541 cggatcttaa aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat
     6601 aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata
     6661 taataaaact taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa
     6721 cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc
     6781 tagggttaat ctagtatcgt agattaatta atccagcttt tgttcccttt agtgagggtt
     6841 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6901 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6961 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7021 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7081 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7141 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7201 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7261 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7321 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7381 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7441 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7501 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7561 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7621 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7681 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7741 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7801 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7861 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7921 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7981 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8041 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8101 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8161 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8221 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8281 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8341 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8401 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8461 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8521 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8581 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8641 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8701 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8761 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8821 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8881 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8941 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9001 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     9061 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     9121 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     9181 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     9241 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     9301 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     9361 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     9421 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gt
//
