LOCUS       sequence-485621-       13931 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Expression of full-length human ARID1A using piggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13931)
  AUTHORS   Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, 
            Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, 
            Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, 
            Cermakova K, Hodges HC
  TITLE     beta-catenin functions as a molecular adapter for disordered cBAF 
            interactions.
  JOURNAL   Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026.
  PUBMED    40695292
REFERENCE   2  (bases 1 to 13931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026."
COMMENT     Sequence Label: pPB-CAG-ARID1A-FLAG
FEATURES             Location/Qualifiers
     source          1..13931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     168..187
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        168..186
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   216..250
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        571..950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     gap             1261..1270
                     /estimated_length=10
     primer_bind     2397..2419
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      2510..2519
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             5296..5304
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9377..9442
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    9537..10086
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9681..9698)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9908..9927
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(10557..10574)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10563..10787
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10834..10850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10858..10874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10882..10888,10889..10906,10907..10912))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10927..10948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   11182..11244
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11285..11305)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11285..11303)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11324..11340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11348..11364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11372..11378,11379..11396,11397..11402))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11417..11438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11555..11572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11726..12314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11806..11825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12485..13276,13277..13345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13108..13127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13346..13450)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13476..13931)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13613..13634)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13825..13844
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgctagac
      541 tcgagaagct tgatcggcca cgcggatctc gacattgatt attgactagt tattaatagt
      601 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      661 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      721 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      781 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      841 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      901 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
      961 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     1021 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggcgcgcccc
     1081 agggggggcg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggc
     1141 ccaaaaaagg gggggccccc gcagggtttt tttttttggg gggggggggg ggggggggcc
     1201 cccaaaaaaa aagagcgggc gggggggggg gggggggcgg ccgccccccc cccccccccc
     1261 nnnnnnnnnn ggggggggag gggggggggg gggggggggg cggggggggg gggggggggg
     1321 ggggggcggg gggggggggg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
     1381 cgcgctccga aagtttcctt ttatggcggg gcggcggcgg cggcggccct ataaaaagcg
     1441 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     1501 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     1561 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     1621 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     1681 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     1741 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1801 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     1861 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     1921 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     1981 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     2041 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     2101 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     2161 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     2221 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     2281 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     2341 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     2401 cggcttctgg cgtgtgaccg gcggctctag ccggagcgca cgtcggcagt cggctccctc
     2461 gttgaccgaa tcaccgacct ctctccccag ggggatccac cgggctagcg ccaccatggc
     2521 cgcgcaggtc gcccccgccg ccgccagcag cctgggcaac ccgccgccgc cgccgccctc
     2581 ggagctgaag aaagccgagc agcagcagcg ggaggaggcg gggggcgagg cggcggcggc
     2641 ggcagcggcc gagcgcgggg aaatgaaggc agccgccggg caggaaagcg agggccccgc
     2701 cgtggggccg ccgcagccgc tgggaaagga gctgcaggac ggggccgaga gcaatggggg
     2761 tggcggcggc ggcggagccg gcagcggcgg cgggcccggc gcggagccgg atctcaaaaa
     2821 cagtaacgga aacgctggcc ctaggcccgc cctgaacaat aacctcacgg agccgcccgg
     2881 cggcggcggt ggcggcagca gcgatggggt gggggcgcct cctcactcag ccgcggccgc
     2941 cttgccgccc ccagcctacg gcttcgggca accctacggc cggagcccgt ctgccgtcgc
     3001 cgccgccgcg gccgccgtct tccaccaaca acatggcgga caacaaagcc ctggcctggc
     3061 agcgctgcag agcggcggcg gcgggggcct ggagccctac gcggggcccc agcagaactc
     3121 tcacgaccac ggcttcccca accaccagta caactcctac taccccaacc gcagcgccta
     3181 ccccccgccc gccccggcct acgcgctgag ctccccgaga ggtggcactc cgggctccgg
     3241 cgcggcggcg gctgccggct ccaagccgcc tccctcctcc agcgcctccg cctcctcgtc
     3301 gtcttcgtcc ttcgctcagc agcgcttcgg ggccatgggg ggaggcggcc cctccgcggc
     3361 cggcggggga actccccagc ccaccgccac ccccaccctc aaccaactgc tcacgtcgcc
     3421 cagctcggcc cggggctacc agggctaccc cgggggcgac tacagtggcg ggccccagga
     3481 cgggggcgcc ggcaagggcc cggcggacat ggcctcgcag tgttgggggg ctgcggcggc
     3541 ggcagctgcg gcggcggccg cctcgggagg ggcccaacaa aggagccacc acgcgcccat
     3601 gagccccggg agcagcggcg gcggggggca gccgctcgcc cggacccctc agccatccag
     3661 tccaatggat cagatgggca agatgagacc tcagccatat ggcgggacta acccatactc
     3721 gcagcaacag ggacctccgt caggaccgca gcaaggacat gggtacccag ggcagccata
     3781 cgggtcccag accccgcagc ggtacccgat gaccatgcag ggccgggcgc agagtgccat
     3841 gggcggcctc tcttatacac agcagattcc tccttatgga caacaaggcc ccagcgggta
     3901 tggtcaacag ggccagactc catattacaa ccagcaaagt cctcaccctc agcagcagca
     3961 gccaccctac tcccagcaac caccgtccca gacccctcat gcccaacctt cgtatcagca
     4021 gcagccacag tctcaaccac cacagctcca gtcctctcag cctccatact cccagcagcc
     4081 atcccagcct ccacatcagc agtccccggc tccatacccc tcccagcagt cgacgacaca
     4141 gcagcacccc cagagccagc ccccctactc acagccacag gctcagtctc cttaccagca
     4201 gcagcaacct cagcagccag caccctcgac gctctcccag caggctgcgt atcctcagcc
     4261 ccagtctcag cagtcccagc aaactgccta ttcccagcag cgcttccctc caccgcagga
     4321 gctatctcaa gattcatttg ggtctcaggc atcctcagct cctagcatga caagctctaa
     4381 aggagggcaa gaagatatga acctgagcct tcagtcaaga cccagttctc tcccagactt
     4441 gtcaggttct atagatgacc tccccatggg gacagaagga gctctgagtc ctggagtgag
     4501 cacatcaggg atttccagca gccaaggaga gcagagtaat ccagctcagt ctcctttctc
     4561 tcctcatacc tcccctcacc tgcctggcat ccgaggccct tccccgtccc ctgttggctc
     4621 tcccgccagt gttgctcagt ctcgctcagg accactctcg cctgctgcag tgccaggcaa
     4681 ccagatgcca cctcggccac ccagtggcca gtcggacagc atcatgcatc cttccatgaa
     4741 ccaatcaagc attgcccaag atcgaggtta tatgcagagg aacccccaga tgccccagta
     4801 cagttccccc cagcccggct cagccttatc tccgcgtcag ccttccggag gacagataca
     4861 cacaggcatg ggctcctacc agcagaactc catggggagc tatggtcccc aggggggtca
     4921 gtatggccca caaggtggct accccaggca gccaaactat aatgccttgc ccaatgccaa
     4981 ctaccccagt gcaggcatgg ctggaggcat aaaccccatg ggtgccggag gtcaaatgca
     5041 tggacagcct ggcatcccac cttatggcac actccctcca gggaggatga gtcacgcctc
     5101 catgggcaac cggccttatg gccctaacat ggccaatatg ccacctcagg ttgggtcagg
     5161 gatgtgtccc ccaccagggg gcatgaaccg gaaaacccaa gaaactgctg tcgccatgca
     5221 tgttgctgcc aactctatcc aaaacaggcc gccaggctac cccaatatga atcaaggggg
     5281 catgatggga actggacctc cttatggaca agggattaat agtatggctg gcatgatcaa
     5341 ccctcaggga cccccatatt ccatgggtgg aaccatggcc aacaattctg cagggatggc
     5401 agccagccca gagatgatgg gccttgggga tgtaaagtta actccagcca ccaaaatgaa
     5461 caacaaggca gatgggacac ccaagacaga atccaaatcc aagaaatcca gttcttctac
     5521 tacaaccaat gagaagatca ccaagttgta tgagctgggt ggtgagcctg agaggaagat
     5581 gtgggtggac cgttatctgg ccttcactga ggagaaggcc atgggcatga caaatctgcc
     5641 tgctgtgggt aggaaacctc tggacctcta tcgcctctat gtgtctgtga aggagattgg
     5701 tggattgact caggtcaaca agaacaaaaa atggcgggaa cttgcaacca acctcaatgt
     5761 gggcacatca agcagtgctg ccagctcctt gaaaaagcag tatatccagt gtctctatgc
     5821 ctttgaatgc aagattgaac ggggagaaga ccctccccca gacatctttg cagctgctga
     5881 ttccaagaag tcccagccca agatccagcc tccctctcct gcgggatcag gatctatgca
     5941 ggggccccag actccccagt caaccagcag ttccatggca gaaggaggag acttaaagcc
     6001 accaactcca gcatccacac cacacagtca gatcccccca ttgccaggca tgagcaggag
     6061 caattcagtt gggatccagg atgcctttaa tgatggaagt gactccacat tccagaagcg
     6121 gaattccatg actccaaacc ctgggtatca gcccagtatg aatacctctg acatgatggg
     6181 gcgcatgtcc tatgagccaa ataaggatcc ttatggcagc atgaggaaag ctccagggag
     6241 tgatcccttc atgtcctcag ggcagggccc caacggcggg atgggtgacc cctacagtcg
     6301 tgctgccggc cctgggctag gaaatgtggc gatgggacca cgacagcact atccctatgg
     6361 aggtccttat gacagagtga ggacggagcc tggaataggg cctgagggaa acatgagcac
     6421 tggggcccca cagccgaatc tcatgccttc caacccagac tcggggatgt attctcctag
     6481 ccgctacccc ccgcagcagc agcagcagca gcagcaacga catgattcct atggcaatca
     6541 gttctccacc caaggcaccc cttctggcag ccccttcccc agccagcaga ctacaatgta
     6601 tcaacagcaa cagcagaatt acaagcggcc aatggatggc acatatggcc ctcctgccaa
     6661 gcggcacgaa ggggagatgt acagcgtgcc atacagcact gggcaggggc agcctcagca
     6721 gcagcagttg cccccagccc agccccagcc tgccagccag caacaagctg cccagccttc
     6781 ccctcagcaa gatgtataca accagtatgg caatgcctat cctgccactg ccacagctgc
     6841 tactgagcgc cgaccagcag gcggccccca gaaccaattt ccattccagt ttggccgaga
     6901 ccgtgtctct gcaccccctg gcaccaatgc ccagcaaaac atgccaccac aaatgatggg
     6961 cggccccata caggcatcag ctgaggttgc tcagcaaggc accatgtggc aggggcgtaa
     7021 tgacatgacc tataattatg ccaacaggca gagcacgggc tctgcccccc agggccccgc
     7081 ctatcatggc gtgaaccgaa cagatgaaat gctgcacaca gatcagaggg ccaaccacga
     7141 aggctcgtgg ccttcccatg gcacacgcca gcccccatat ggtccctctg cccctgtgcc
     7201 ccccatgaca aggccccctc catctaacta ccagccccca ccaagcatgc agaatcacat
     7261 tcctcaggta tccagccctg ctcccctgcc ccggccaatg gagaaccgca cctctcctag
     7321 caagtctcca ttcctgcact ctgggatgaa aatgcagaag gcaggtcccc cagtacctgc
     7381 ctcgcacata gcacctgccc ctgtgcagcc ccccatgatt cggcgggata tcaccttccc
     7441 acctggctct gttgaagcca cacagcctgt gttgaagcag aggaggcggc tcacaatgaa
     7501 agacattgga accccggagg catggcgggt aatgatgtcc ctcaagtctg gtctcctggc
     7561 agagagcaca tgggcattag ataccatcaa catcctgctg tatgatgaca acagcatcat
     7621 gaccttcaac ctcagtcagc tcccagggtt gctagagctc cttgtagaat atttccgacg
     7681 atgcctgatt gagatctttg gcattttaaa ggagtatgag gtgggtgacc caggacagag
     7741 aacgctactg gatcctggga ggttcagcaa ggtgtctagt ccagctccca tggagggtgg
     7801 ggaagaagaa gaagaacttc taggtcctaa actagaagag gaagaagaag aggaagtagt
     7861 tgaaaatgat gaggagatag ccttttcagg caaggacaag ccagcttcag agaatagtga
     7921 ggagaagctg atcagtaagt ttgacaagct tccagtaaag atcgtacaga agaatgatcc
     7981 atttgtggtg gactgctcag ataagcttgg gcgtgtgcag gagtttgaca gtggcctgct
     8041 gcactggcgg attggtgggg gggacaccac tgagcatatc cagacccact tcgagagcaa
     8101 gacagagctg ctgccttccc ggcctcacgc accctgccca ccagcccctc ggaagcatgt
     8161 gacaacagca gagggtacac cagggacaac agaccaggag gggcccccac ctgatggacc
     8221 tccagaaaaa cggatcacag ccactatgga tgacatgttg tctactcggt ctagcacctt
     8281 gaccgaggat ggagctaaga gttcagaggc catcaaggag agcagcaagt ttccatttgg
     8341 cattagccca gcacagagcc accggaacat caagatccta gaggacgaac cccacagtaa
     8401 ggatgagacc ccactgtgta cccttctgga ctggcaggat tctcttgcca agcgctgcgt
     8461 ctgtgtgtcc aataccattc gaagcctgtc atttgtgcca ggcaatgact ttgagatgtc
     8521 caaacaccca gggctgctgc tcatcctggg caagctgatc ctgctgcacc acaagcaccc
     8581 agaacggaag caggcaccac taacttatga aaaggaggag gaacaggacc aaggggtgag
     8641 ctgcaacaaa gtggagtggt ggtgggactg cttggagatg ctccgggaaa acaccttggt
     8701 tacactcgcc aacatctcgg ggcagttgga cctatctcca taccccgaga gcatttgcct
     8761 gcctgtcctg gacggactcc tacactgggc agtttgccct tcagctgaag cccaggaccc
     8821 cttttccacc ctgggcccca atgccgtcct ttccccgcag agactggtct tggaaaccct
     8881 cagcaaactc agcatccagg acaacaatgt ggacctgatt ctggccacac cccccttcag
     8941 ccgcctggag aagttgtata gcactatggt gcgcttcctc agtgaccgaa agaacccggt
     9001 gtgccgggag atggctgtgg tactgctggc caacctggct cagggggaca gcctggcagc
     9061 tcgtgccatt gcagtgcaga agggcagtat cggcaacctc ctgggcttcc tagaggacag
     9121 ccttgccgcc acacagttcc agcagagcca ggccagcctc ctccacatgc agaacccacc
     9181 ctttgagcca actagtgtgg acatgatgcg gcgggctgcc cgcgcgctgc ttgccttggc
     9241 caaggtggac gagaaccact cagagtttac tctgtacgaa tcacggctgt tggacatctc
     9301 ggtatcaccg ctgatgaata gcctcgtgtc acaagtcatt tgtgatgtac tgtttttgat
     9361 tggccagtcc accggtgact acaaagacca tgacggtgat tataaagatc atgatatcga
     9421 ttacaaggat gacgatgaca agtgaacgcg ttagtgcgga tcctagtgcg aattctagtg
     9481 cgtcgactct agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt
     9541 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     9601 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     9661 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     9721 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     9781 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     9841 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     9901 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     9961 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
    10021 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
    10081 ataataccat ggtcatgaaa acatttaaca tttctcaaca agatctagaa ttagtagaag
    10141 tagcgacaga gaagattaca atgctttatg aggataataa acatcatgtg ggagcggcaa
    10201 ttcgtacgaa aacaggagaa atcatttcgg cagtacatat tgaagcgtat ataggacgag
    10261 taactgtttg tgcagaagcc attgcgattg gtagtgcagt ttcgaatgga caaaaggatt
    10321 ttgacacgat tgtagctgtt agacaccctt attctgacga agtagataga agtattcgag
    10381 tggtaagtcc ttgtggtatg tgtagggagt tgatttcaga ctatgcacca gattgttttg
    10441 tgttaataga aatgaatggc aagttagtca aaactacgat tgaagaactc attccactca
    10501 aatatacccg aaattaaaag tcgagatgca tcgatgatct agagctcgct gatcagcctc
    10561 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
    10621 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    10681 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
    10741 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
    10801 aagaacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    10861 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    10921 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    10981 gggaaacctg tcgtgccagc ggatcttaaa agttttgtta ctttatagaa gaaattttga
    11041 gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat
    11101 tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc
    11161 gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt
    11221 cacaatatga ttatctttct agggttaatc tagtatcgta gattaattaa tccagctttt
    11281 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
    11341 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    11401 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    11461 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    11521 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    11581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    11641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    11701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    11761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    11821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    11881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    11941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    12001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    12061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    12121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    12181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    12241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    12301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    12361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    12421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    12481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    12541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    12601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    12661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    12721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    12781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    12841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    12901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    12961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    13021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    13081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    13141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    13201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    13261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    13321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    13381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    13441 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
    13501 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
    13561 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
    13621 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
    13681 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
    13741 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
    13801 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
    13861 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
    13921 acagggcgcg t
//
