LOCUS       sequence-485623-       11782 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Expression of human ARID1A ∆ARID and ∆IDR2 (deletion of a.a. 
            971-1610) using piggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, 
            Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, 
            Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, 
            Cermakova K, Hodges HC
  TITLE     beta-catenin functions as a molecular adapter for disordered cBAF 
            interactions.
  JOURNAL   Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026.
  PUBMED    40695292
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026."
COMMENT     Sequence Label: pPB-CAG-ARID1A-deltaARIDIDR2-FLAG
FEATURES             Location/Qualifiers
     source          1..11782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     168..187
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        168..186
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   216..250
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        571..950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        952..1227
                     /label=chicken beta-actin promoter
     primer_bind     2168..2190
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      2281..2290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             5067..5075
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7228..7293
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7388..7937
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7532..7549)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7759..7778
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(8408..8425)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8414..8638
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8685..8701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8709..8725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8733..8739,8740..8757,8758..8763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8778..8799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   9033..9095
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9136..9156)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9136..9154)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9175..9191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9199..9215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9223..9229,9230..9247,9248..9253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9268..9289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9406..9423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9577..10165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9657..9676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10336..11127,11128..11196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10959..10978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11197..11301)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11327..11782)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11464..11485)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11676..11695
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgctagac
      541 tcgagaagct tgatcggcca cgcggatctc gacattgatt attgactagt tattaatagt
      601 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      661 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      721 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      781 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      841 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      901 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
      961 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     1021 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca
     1081 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
     1141 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
     1201 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
     1261 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     1321 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     1381 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     1441 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     1501 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     1561 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     1621 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     1681 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     1741 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     1801 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     1861 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     1921 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     1981 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     2041 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     2101 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     2161 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gccggagcgc acgtcggcag
     2221 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca ccgggctagc
     2281 gccaccatgg ccgcgcaggt cgcccccgcc gccgccagca gcctgggcaa cccgccgccg
     2341 ccgccgccct cggagctgaa gaaagccgag cagcagcagc gggaggaggc ggggggcgag
     2401 gcggcggcgg cggcagcggc cgagcgcggg gaaatgaagg cagccgccgg gcaggaaagc
     2461 gagggccccg ccgtggggcc gccgcagccg ctgggaaagg agctgcagga cggggccgag
     2521 agcaatgggg gtggcggcgg cggcggagcc ggcagcggcg gcgggcccgg cgcggagccg
     2581 gatctcaaaa acagtaacgg aaacgctggc cctaggcccg ccctgaacaa taacctcacg
     2641 gagccgcccg gcggcggcgg tggcggcagc agcgatgggg tgggggcgcc tcctcactca
     2701 gccgcggccg ccttgccgcc cccagcctac ggcttcgggc aaccctacgg ccggagcccg
     2761 tctgccgtcg ccgccgccgc ggccgccgtc ttccaccaac aacatggcgg acaacaaagc
     2821 cctggcctgg cagcgctgca gagcggcggc ggcgggggcc tggagcccta cgcggggccc
     2881 cagcagaact ctcacgacca cggcttcccc aaccaccagt acaactccta ctaccccaac
     2941 cgcagcgcct accccccgcc cgccccggcc tacgcgctga gctccccgag aggtggcact
     3001 ccgggctccg gcgcggcggc ggctgccggc tccaagccgc ctccctcctc cagcgcctcc
     3061 gcctcctcgt cgtcttcgtc cttcgctcag cagcgcttcg gggccatggg gggaggcggc
     3121 ccctccgcgg ccggcggggg aactccccag cccaccgcca cccccaccct caaccaactg
     3181 ctcacgtcgc ccagctcggc ccggggctac cagggctacc ccgggggcga ctacagtggc
     3241 gggccccagg acgggggcgc cggcaagggc ccggcggaca tggcctcgca gtgttggggg
     3301 gctgcggcgg cggcagctgc ggcggcggcc gcctcgggag gggcccaaca aaggagccac
     3361 cacgcgccca tgagccccgg gagcagcggc ggcggggggc agccgctcgc ccggacccct
     3421 cagccatcca gtccaatgga tcagatgggc aagatgagac ctcagccata tggcgggact
     3481 aacccatact cgcagcaaca gggacctccg tcaggaccgc agcaaggaca tgggtaccca
     3541 gggcagccat acgggtccca gaccccgcag cggtacccga tgaccatgca gggccgggcg
     3601 cagagtgcca tgggcggcct ctcttataca cagcagattc ctccttatgg acaacaaggc
     3661 cccagcgggt atggtcaaca gggccagact ccatattaca accagcaaag tcctcaccct
     3721 cagcagcagc agccacccta ctcccagcaa ccaccgtccc agacccctca tgcccaacct
     3781 tcgtatcagc agcagccaca gtctcaacca ccacagctcc agtcctctca gcctccatac
     3841 tcccagcagc catcccagcc tccacatcag cagtccccgg ctccataccc ctcccagcag
     3901 tcgacgacac agcagcaccc ccagagccag cccccctact cacagccaca ggctcagtct
     3961 ccttaccagc agcagcaacc tcagcagcca gcaccctcga cgctctccca gcaggctgcg
     4021 tatcctcagc cccagtctca gcagtcccag caaactgcct attcccagca gcgcttccct
     4081 ccaccgcagg agctatctca agattcattt gggtctcagg catcctcagc tcctagcatg
     4141 acaagctcta aaggagggca agaagatatg aacctgagcc ttcagtcaag acccagttct
     4201 ctcccagact tgtcaggttc tatagatgac ctccccatgg ggacagaagg agctctgagt
     4261 cctggagtga gcacatcagg gatttccagc agccaaggag agcagagtaa tccagctcag
     4321 tctcctttct ctcctcatac ctcccctcac ctgcctggca tccgaggccc ttccccgtcc
     4381 cctgttggct ctcccgccag tgttgctcag tctcgctcag gaccactctc gcctgctgca
     4441 gtgccaggca accagatgcc acctcggcca cccagtggcc agtcggacag catcatgcat
     4501 ccttccatga accaatcaag cattgcccaa gatcgaggtt atatgcagag gaacccccag
     4561 atgccccagt acagttcccc ccagcccggc tcagccttat ctccgcgtca gccttccgga
     4621 ggacagatac acacaggcat gggctcctac cagcagaact ccatggggag ctatggtccc
     4681 caggggggtc agtatggccc acaaggtggc taccccaggc agccaaacta taatgccttg
     4741 cccaatgcca actaccccag tgcaggcatg gctggaggca taaaccccat gggtgccgga
     4801 ggtcaaatgc atggacagcc tggcatccca ccttatggca cactccctcc agggaggatg
     4861 agtcacgcct ccatgggcaa ccggccttat ggccctaaca tggccaatat gccacctcag
     4921 gttgggtcag ggatgtgtcc cccaccaggg ggcatgaacc ggaaaaccca agaaactgct
     4981 gtcgccatgc atgttgctgc caactctatc caaaacaggc cgccaggcta ccccaatatg
     5041 aatcaagggg gcatgatggg aactggacct ccttatggac aagggattaa tagtatggct
     5101 ggcatgatca accctcaggg acccccatat tccatgggtg gaaccatggc caacaattct
     5161 gcagggatgg cagccagccc agagatgatg ggccttatga aaatgcagaa ggcaggtccc
     5221 ccagtacctg cctcgcacat agcacctgcc cctgtgcagc cccccatgat tcggcgggat
     5281 atcaccttcc cacctggctc tgttgaagcc acacagcctg tgttgaagca gaggaggcgg
     5341 ctcacaatga aagacattgg aaccccggag gcatggcggg taatgatgtc cctcaagtct
     5401 ggtctcctgg cagagagcac atgggcatta gataccatca acatcctgct gtatgatgac
     5461 aacagcatca tgaccttcaa cctcagtcag ctcccagggt tgctagagct ccttgtagaa
     5521 tatttccgac gatgcctgat tgagatcttt ggcattttaa aggagtatga ggtgggtgac
     5581 ccaggacaga gaacgctact ggatcctggg aggttcagca aggtgtctag tccagctccc
     5641 atggagggtg gggaagaaga agaagaactt ctaggtccta aactagaaga ggaagaagaa
     5701 gaggaagtag ttgaaaatga tgaggagata gccttttcag gcaaggacaa gccagcttca
     5761 gagaatagtg aggagaagct gatcagtaag tttgacaagc ttccagtaaa gatcgtacag
     5821 aagaatgatc catttgtggt ggactgctca gataagcttg ggcgtgtgca ggagtttgac
     5881 agtggcctgc tgcactggcg gattggtggg ggggacacca ctgagcatat ccagacccac
     5941 ttcgagagca agacagagct gctgccttcc cggcctcacg caccctgccc accagcccct
     6001 cggaagcatg tgacaacagc agagggtaca ccagggacaa cagaccagga ggggccccca
     6061 cctgatggac ctccagaaaa acggatcaca gccactatgg atgacatgtt gtctactcgg
     6121 tctagcacct tgaccgagga tggagctaag agttcagagg ccatcaagga gagcagcaag
     6181 tttccatttg gcattagccc agcacagagc caccggaaca tcaagatcct agaggacgaa
     6241 ccccacagta aggatgagac cccactgtgt acccttctgg actggcagga ttctcttgcc
     6301 aagcgctgcg tctgtgtgtc caataccatt cgaagcctgt catttgtgcc aggcaatgac
     6361 tttgagatgt ccaaacaccc agggctgctg ctcatcctgg gcaagctgat cctgctgcac
     6421 cacaagcacc cagaacggaa gcaggcacca ctaacttatg aaaaggagga ggaacaggac
     6481 caaggggtga gctgcaacaa agtggagtgg tggtgggact gcttggagat gctccgggaa
     6541 aacaccttgg ttacactcgc caacatctcg gggcagttgg acctatctcc ataccccgag
     6601 agcatttgcc tgcctgtcct ggacggactc ctacactggg cagtttgccc ttcagctgaa
     6661 gcccaggacc ccttttccac cctgggcccc aatgccgtcc tttccccgca gagactggtc
     6721 ttggaaaccc tcagcaaact cagcatccag gacaacaatg tggacctgat tctggccaca
     6781 ccccccttca gccgcctgga gaagttgtat agcactatgg tgcgcttcct cagtgaccga
     6841 aagaacccgg tgtgccggga gatggctgtg gtactgctgg ccaacctggc tcagggggac
     6901 agcctggcag ctcgtgccat tgcagtgcag aagggcagta tcggcaacct cctgggcttc
     6961 ctagaggaca gccttgccgc cacacagttc cagcagagcc aggccagcct cctccacatg
     7021 cagaacccac cctttgagcc aactagtgtg gacatgatgc ggcgggctgc ccgcgcgctg
     7081 cttgccttgg ccaaggtgga cgagaaccac tcagagttta ctctgtacga atcacggctg
     7141 ttggacatct cggtatcacc gctgatgaat agcctcgtgt cacaagtcat ttgtgatgta
     7201 ctgtttttga ttggccagtc caccggtgac tacaaagacc atgacggtga ttataaagat
     7261 catgatatcg attacaagga tgacgatgac aagtgaacgc gttagtgcgg atcctagtgc
     7321 gaattctagt gcgtcgactc tagcaacggt ttccctctag cgggatcaat tccgcccccc
     7381 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     7441 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     7501 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     7561 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     7621 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     7681 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     7741 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     7801 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     7861 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     7921 tgaaaaacac gataatacca tggtcatgaa aacatttaac atttctcaac aagatctaga
     7981 attagtagaa gtagcgacag agaagattac aatgctttat gaggataata aacatcatgt
     8041 gggagcggca attcgtacga aaacaggaga aatcatttcg gcagtacata ttgaagcgta
     8101 tataggacga gtaactgttt gtgcagaagc cattgcgatt ggtagtgcag tttcgaatgg
     8161 acaaaaggat tttgacacga ttgtagctgt tagacaccct tattctgacg aagtagatag
     8221 aagtattcga gtggtaagtc cttgtggtat gtgtagggag ttgatttcag actatgcacc
     8281 agattgtttt gtgttaatag aaatgaatgg caagttagtc aaaactacga ttgaagaact
     8341 cattccactc aaatataccc gaaattaaaa gtcgagatgc atcgatgatc tagagctcgc
     8401 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8461 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8521 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     8581 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     8641 tctgaggcgg aaagaacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     8701 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8761 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8821 gctttccagt cgggaaacct gtcgtgccag cggatcttaa aagttttgtt actttataga
     8881 agaaattttg agtttttgtt tttttttaat aaataaataa acataaataa attgtttgtt
     8941 gaatttatta ttagtatgta agtgtaaata taataaaact taatatctat tcaaattaat
     9001 aaataaacct cgatatacag accgataaaa cacatgcgtc aattttacgc atgattatct
     9061 ttaacgtacg tcacaatatg attatctttc tagggttaat ctagtatcgt agattaatta
     9121 atccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     9181 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9241 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9301 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9361 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9421 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9481 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9541 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10081 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10141 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10201 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10321 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10381 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10441 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10501 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10561 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10621 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10681 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10741 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10801 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10861 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10921 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10981 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11041 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11101 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11161 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11221 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11281 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
    11341 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
    11401 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
    11461 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
    11521 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
    11581 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
    11641 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
    11701 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
    11761 aatgcgccgc tacagggcgc gt
//
