LOCUS       sequence-485626-        9217 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Expression of full-length human beta-catenin using piggyBac 
            transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, 
            Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, 
            Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, 
            Cermakova K, Hodges HC
  TITLE     beta-catenin functions as a molecular adapter for disordered cBAF 
            interactions.
  JOURNAL   Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026.
  PUBMED    40695292
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026."
COMMENT     Sequence Label: pPB-CAG-CTNNB1-HA
FEATURES             Location/Qualifiers
     source          1..9217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     343..362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(581..685)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(711..1166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(848..869)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1060..1079
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1293..1315
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1307..1324
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1308..1324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1334..1353
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1334..1352
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1382..1416
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1737..2116
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2118..2393
                     /label=chicken beta-actin promoter
     primer_bind     3334..3356
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      3447..3456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3453..5795
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             3453..5795
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             5805..5894
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    5989..6538
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6133..6150)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6360..6379
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7009..7026)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7015..7239
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7286..7302)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7310..7326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7334..7340,7341..7358,7359..7364))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7379..7400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7634..7696
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7737..7757)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7737..7755)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7776..7792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7800..7816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7824..7830,7831..7848,7849..7854))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7869..7890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8007..8024)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8178..8766)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8258..8277)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8937..9217,1..511,512..580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
       61 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
      121 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
      181 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
      241 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
      301 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
      361 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
      421 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
      481 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
      541 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
      601 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
      661 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
      721 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
      781 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      841 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      901 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      961 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     1021 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     1081 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     1141 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     1201 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1261 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1321 cagtgagcgc gcgtaatacg actcactata gggcgaattg gggcgcgcca ttctagatta
     1381 accctagaaa gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct
     1441 ttctaaatag cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct
     1501 cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg
     1561 tgagtcaaaa tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata
     1621 attatattgt tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt
     1681 tatagatatc aactagaatg ctagactcga gaagcttgat cggccacgcg gatctcgaca
     1741 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1801 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1861 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1921 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1981 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2041 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2101 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     2161 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     2221 ggcggggggg gggggggggg gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     2281 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     2341 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     2401 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     2461 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     2521 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     2581 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     2641 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     2701 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     2761 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     2821 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     2881 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     2941 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     3001 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     3061 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     3121 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     3181 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     3241 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     3301 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     3361 gctctagccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     3421 tccccagggg gatccaccgg gctagcgcca ccatggctac tcaagctgat ttgatggagt
     3481 tggacatggc catggaacca gacagaaaag cggctgttag tcactggcag caacagtctt
     3541 acctggactc tggaatccat tctggtgcca ctaccacagc tccttctctg agtggtaaag
     3601 gcaatcctga ggaagaggat gtggatacct cccaagtcct gtatgagtgg gaacagggat
     3661 tttctcagtc cttcactcaa gaacaagtag ctgatattga tggacagtat gcaatgactc
     3721 gagctcagag ggtacgagct gctatgttcc ctgagacatt agatgagggc atgcagatcc
     3781 catctacaca gtttgatgct gctcatccca ctaatgtcca gcgtttggct gaaccatcac
     3841 agatgctgaa acatgcagtt gtaaacttga ttaactatca agatgatgca gaacttgcca
     3901 cacgtgcaat ccctgaactg acaaaactgc taaatgacga ggaccaggtg gtggttaata
     3961 aggctgcagt tatggtccat cagctttcta aaaaggaagc ttccagacac gctatcatgc
     4021 gttctcctca gatggtgtct gctattgtac gtaccatgca gaatacaaat gatgtagaaa
     4081 cagctcgttg taccgctggg accttgcata acctttccca tcatcgtgag ggcttactgg
     4141 ccatctttaa gtctggaggc attcctgccc tggtgaaaat gcttggttca ccagtggatt
     4201 ctgtgttgtt ttatgccatt acaactctcc acaacctttt attacatcaa gaaggagcta
     4261 aaatggcagt gcgtttagct ggtgggctgc agaaaatggt tgccttgctc aacaaaacaa
     4321 atgttaaatt cttggctatt acgacagact gccttcaaat tttagcttat ggcaaccaag
     4381 aaagcaagct catcatactg gctagtggtg gaccccaagc tttagtaaat ataatgagga
     4441 cctatactta cgaaaaacta ctgtggacca caagcagagt gctgaaggtg ctatctgtct
     4501 gctctagtaa taagccggct attgtagaag ctggtggaat gcaagcttta ggacttcacc
     4561 tgacagatcc aagtcaacgt cttgttcaga actgtctttg gactctcagg aatctttcag
     4621 atgctgcaac taaacaggaa gggatggaag gtctccttgg gactcttgtt cagcttctgg
     4681 gttcagatga tataaatgtg gtcacctgtg cagctggaat tctttctaac ctcacttgca
     4741 ataattataa gaacaagatg atggtctgcc aagtgggtgg tatagaggct cttgtgcgta
     4801 ctgtccttcg ggctggtgac agggaagaca tcactgagcc tgccatctgt gctcttcgtc
     4861 atctgaccag ccgacaccaa gaagcagaga tggcccagaa tgcagttcgc cttcactatg
     4921 gactaccagt tgtggttaag ctcttacacc caccatccca ctggcctctg ataaaggcta
     4981 ctgttggatt gattcgaaat cttgcccttt gtcccgcaaa tcatgcacct ttgcgtgagc
     5041 agggtgccat tccacgacta gttcagttgc ttgttcgtgc acatcaggat acccagcgcc
     5101 gtacgtccat gggtgggaca cagcagcaat ttgtggaggg ggtccgcatg gaagaaatag
     5161 ttgaaggttg taccggagcc cttcacatcc tagctcggga tgttcacaac cgaattgtta
     5221 tcagaggact aaataccatt ccattgtttg tgcagctgct ttattctccc attgaaaaca
     5281 tccaaagagt agctgcaggg gtcctctgtg aacttgctca ggacaaggaa gctgcagaag
     5341 ctattgaagc tgagggagcc acagctcctc tgacagagtt acttcactct aggaatgaag
     5401 gtgtggcgac atatgcagct gctgttttgt tccgaatgtc tgaggacaag ccacaagatt
     5461 acaagaaacg gctttcagtt gagctgacca gctctctctt cagaacagag ccaatggctt
     5521 ggaatgagac tgctgatctt ggacttgata ttggtgccca gggagaaccc cttggatatc
     5581 gccaggatga tcctagctat cgttcttttc actctggtgg atatggccag gatgccttgg
     5641 gtatggaccc catgatggaa catgagatgg gtggccacca ccctggtgct gactatccag
     5701 ttgatgggct gccagatctg gggcatgccc aggacctcat ggatgggctg cctccaggtg
     5761 acagcaatca gctggcctgg tttgatactg acctgaccgg tatgtaccca tacgatgttc
     5821 cagattacgc tggctatccc tatgacgtcc cggactatgc aggctcctat ccatatgacg
     5881 ttccagatta cgcttgaacg cgttagtgcg gatcctagtg cgaattctag tgcgtcgact
     5941 ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc
     6001 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     6061 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     6121 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     6181 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     6241 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     6301 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     6361 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     6421 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     6481 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
     6541 atggtcatga aaacatttaa catttctcaa caagatctag aattagtaga agtagcgaca
     6601 gagaagatta caatgcttta tgaggataat aaacatcatg tgggagcggc aattcgtacg
     6661 aaaacaggag aaatcatttc ggcagtacat attgaagcgt atataggacg agtaactgtt
     6721 tgtgcagaag ccattgcgat tggtagtgca gtttcgaatg gacaaaagga ttttgacacg
     6781 attgtagctg ttagacaccc ttattctgac gaagtagata gaagtattcg agtggtaagt
     6841 ccttgtggta tgtgtaggga gttgatttca gactatgcac cagattgttt tgtgttaata
     6901 gaaatgaatg gcaagttagt caaaactacg attgaagaac tcattccact caaatatacc
     6961 cgaaattaaa agtcgagatg catcgatgat ctagagctcg ctgatcagcc tcgactgtgc
     7021 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     7081 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     7141 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     7201 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacct
     7261 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7321 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7381 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7441 tgtcgtgcca gcggatctta aaagttttgt tactttatag aagaaatttt gagtttttgt
     7501 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     7561 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
     7621 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
     7681 gattatcttt ctagggttaa tctagtatcg tagattaatt aatccagctt ttgttccctt
     7741 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7801 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7861 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7921 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7981 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8041 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8101 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8161 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8221 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8281 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8341 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8401 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8461 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8521 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8581 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8641 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8701 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8761 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8821 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8881 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8941 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9001 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9061 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9121 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9181 attaattgtt gccgggaagc tagagtaagt agttcgc
//
