LOCUS       sequence-485630-       10963 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Expression of human ARID1A ∆IDR1 (deletion of a.a. 2–970) using 
            piggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, 
            Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, 
            Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, 
            Cermakova K, Hodges HC
  TITLE     beta-catenin functions as a molecular adapter for disordered cBAF 
            interactions.
  JOURNAL   Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026.
  PUBMED    40695292
REFERENCE   2  (bases 1 to 10963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026."
COMMENT     Sequence Label: pPB-CAG-ARID1A-deltaIDR1-FLAG
FEATURES             Location/Qualifiers
     source          1..10963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     168..187
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        168..186
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   216..250
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        571..950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     gap             1261..1270
                     /estimated_length=10
     primer_bind     2336..2358
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      2449..2458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6409..6474
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6569..7118
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6713..6730)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6940..6959
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7589..7606)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7595..7819
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7866..7882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7890..7906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7914..7920,7921..7938,7939..7944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7959..7980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8214..8276
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8317..8337)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8317..8335)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8356..8372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8380..8396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8404..8410,8411..8428,8429..8434))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8449..8470
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8587..8604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8758..9346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8838..8857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9517..10308,10309..10377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10140..10159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10378..10482)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10508..10963)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10645..10666)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10857..10876
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgctagac
      541 tcgagaagct tgatcggcca cgcggatctc gacattgatt attgactagt tattaatagt
      601 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      661 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      721 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      781 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      841 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      901 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
      961 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     1021 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggggcgcgcc
     1081 aggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggc
     1141 caaacagagg gggggggccc ggaaggtttc ctttttgggg gggggggggg gggggggggc
     1201 cccaaaaaaa agaaagggcg cggggggggg ggggggcggc gcggtgcctt cgccccgggc
     1261 nnnnnnnnnn ggcggggggg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     1321 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     1381 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     1441 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     1501 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     1561 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     1621 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     1681 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     1741 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     1801 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     1861 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     1921 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     1981 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     2041 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     2101 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     2161 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     2221 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     2281 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     2341 ggcttctggc gtgtgaccgg cggctctagc cggagcgcac gtcggcagtc ggctccctcg
     2401 ttgaccgaat caccgacctc tctccccagg gggatccacc gggctagcgc caccatgggg
     2461 gatgtaaagt taactccagc caccaaaatg aacaacaagg cagatgggac acccaagaca
     2521 gaatccaaat ccaagaaatc cagttcttct actacaacca atgagaagat caccaagttg
     2581 tatgagctgg gtggtgagcc tgagaggaag atgtgggtgg accgttatct ggccttcact
     2641 gaggagaagg ccatgggcat gacaaatctg cctgctgtgg gtaggaaacc tctggacctc
     2701 tatcgcctct atgtgtctgt gaaggagatt ggtggattga ctcaggtcaa caagaacaaa
     2761 aaatggcggg aacttgcaac caacctcaat gtgggcacat caagcagtgc tgccagctcc
     2821 ttgaaaaagc agtatatcca gtgtctctat gcctttgaat gcaagattga acggggagaa
     2881 gaccctcccc cagacatctt tgcagctgct gattccaaga agtcccagcc caagatccag
     2941 cctccctctc ctgcgggatc aggatctatg caggggcccc agactcccca gtcaaccagc
     3001 agttccatgg cagaaggagg agacttaaag ccaccaactc cagcatccac accacacagt
     3061 cagatccccc cattgccagg catgagcagg agcaattcag ttgggatcca ggatgccttt
     3121 aatgatggaa gtgactccac attccagaag cggaattcca tgactccaaa ccctgggtat
     3181 cagcccagta tgaatacctc tgacatgatg gggcgcatgt cctatgagcc aaataaggat
     3241 ccttatggca gcatgaggaa agctccaggg agtgatccct tcatgtcctc agggcagggc
     3301 cccaacggcg ggatgggtga cccctacagt cgtgctgccg gccctgggct aggaaatgtg
     3361 gcgatgggac cacgacagca ctatccctat ggaggtcctt atgacagagt gaggacggag
     3421 cctggaatag ggcctgaggg aaacatgagc actggggccc cacagccgaa tctcatgcct
     3481 tccaacccag actcggggat gtattctcct agccgctacc ccccgcagca gcagcagcag
     3541 cagcagcaac gacatgattc ctatggcaat cagttctcca cccaaggcac cccttctggc
     3601 agccccttcc ccagccagca gactacaatg tatcaacagc aacagcagaa ttacaagcgg
     3661 ccaatggatg gcacatatgg ccctcctgcc aagcggcacg aaggggagat gtacagcgtg
     3721 ccatacagca ctgggcaggg gcagcctcag cagcagcagt tgcccccagc ccagccccag
     3781 cctgccagcc agcaacaagc tgcccagcct tcccctcagc aagatgtata caaccagtat
     3841 ggcaatgcct atcctgccac tgccacagct gctactgagc gccgaccagc aggcggcccc
     3901 cagaaccaat ttccattcca gtttggccga gaccgtgtct ctgcaccccc tggcaccaat
     3961 gcccagcaaa acatgccacc acaaatgatg ggcggcccca tacaggcatc agctgaggtt
     4021 gctcagcaag gcaccatgtg gcaggggcgt aatgacatga cctataatta tgccaacagg
     4081 cagagcacgg gctctgcccc ccagggcccc gcctatcatg gcgtgaaccg aacagatgaa
     4141 atgctgcaca cagatcagag ggccaaccac gaaggctcgt ggccttccca tggcacacgc
     4201 cagcccccat atggtccctc tgcccctgtg ccccccatga caaggccccc tccatctaac
     4261 taccagcccc caccaagcat gcagaatcac attcctcagg tatccagccc tgctcccctg
     4321 ccccggccaa tggagaaccg cacctctcct agcaagtctc cattcctgca ctctgggatg
     4381 aaaatgcaga aggcaggtcc cccagtacct gcctcgcaca tagcacctgc ccctgtgcag
     4441 ccccccatga ttcggcggga tatcaccttc ccacctggct ctgttgaagc cacacagcct
     4501 gtgttgaagc agaggaggcg gctcacaatg aaagacattg gaaccccgga ggcatggcgg
     4561 gtaatgatgt ccctcaagtc tggtctcctg gcagagagca catgggcatt agataccatc
     4621 aacatcctgc tgtatgatga caacagcatc atgaccttca acctcagtca gctcccaggg
     4681 ttgctagagc tccttgtaga atatttccga cgatgcctga ttgagatctt tggcatttta
     4741 aaggagtatg aggtgggtga cccaggacag agaacgctac tggatcctgg gaggttcagc
     4801 aaggtgtcta gtccagctcc catggagggt ggggaagaag aagaagaact tctaggtcct
     4861 aaactagaag aggaagaaga agaggaagta gttgaaaatg atgaggagat agccttttca
     4921 ggcaaggaca agccagcttc agagaatagt gaggagaagc tgatcagtaa gtttgacaag
     4981 cttccagtaa agatcgtaca gaagaatgat ccatttgtgg tggactgctc agataagctt
     5041 gggcgtgtgc aggagtttga cagtggcctg ctgcactggc ggattggtgg gggggacacc
     5101 actgagcata tccagaccca cttcgagagc aagacagagc tgctgccttc ccggcctcac
     5161 gcaccctgcc caccagcccc tcggaagcat gtgacaacag cagagggtac accagggaca
     5221 acagaccagg aggggccccc acctgatgga cctccagaaa aacggatcac agccactatg
     5281 gatgacatgt tgtctactcg gtctagcacc ttgaccgagg atggagctaa gagttcagag
     5341 gccatcaagg agagcagcaa gtttccattt ggcattagcc cagcacagag ccaccggaac
     5401 atcaagatcc tagaggacga accccacagt aaggatgaga ccccactgtg tacccttctg
     5461 gactggcagg attctcttgc caagcgctgc gtctgtgtgt ccaataccat tcgaagcctg
     5521 tcatttgtgc caggcaatga ctttgagatg tccaaacacc cagggctgct gctcatcctg
     5581 ggcaagctga tcctgctgca ccacaagcac ccagaacgga agcaggcacc actaacttat
     5641 gaaaaggagg aggaacagga ccaaggggtg agctgcaaca aagtggagtg gtggtgggac
     5701 tgcttggaga tgctccggga aaacaccttg gttacactcg ccaacatctc ggggcagttg
     5761 gacctatctc cataccccga gagcatttgc ctgcctgtcc tggacggact cctacactgg
     5821 gcagtttgcc cttcagctga agcccaggac cccttttcca ccctgggccc caatgccgtc
     5881 ctttccccgc agagactggt cttggaaacc ctcagcaaac tcagcatcca ggacaacaat
     5941 gtggacctga ttctggccac accccccttc agccgcctgg agaagttgta tagcactatg
     6001 gtgcgcttcc tcagtgaccg aaagaacccg gtgtgccggg agatggctgt ggtactgctg
     6061 gccaacctgg ctcaggggga cagcctggca gctcgtgcca ttgcagtgca gaagggcagt
     6121 atcggcaacc tcctgggctt cctagaggac agccttgccg ccacacagtt ccagcagagc
     6181 caggccagcc tcctccacat gcagaaccca ccctttgagc caactagtgt ggacatgatg
     6241 cggcgggctg cccgcgcgct gcttgccttg gccaaggtgg acgagaacca ctcagagttt
     6301 actctgtacg aatcacggct gttggacatc tcggtatcac cgctgatgaa tagcctcgtg
     6361 tcacaagtca tttgtgatgt actgtttttg attggccagt ccaccggtga ctacaaagac
     6421 catgacggtg attataaaga tcatgatatc gattacaagg atgacgatga caagtgaacg
     6481 cgttagtgcg gatcctagtg cgaattctag tgcgtcgact ctagcaacgg tttccctcta
     6541 gcgggatcaa ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     6601 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     6661 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     6721 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     6781 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     6841 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     6901 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     6961 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     7021 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     7081 cacggggacg tggttttcct ttgaaaaaca cgataatacc atggtcatga aaacatttaa
     7141 catttctcaa caagatctag aattagtaga agtagcgaca gagaagatta caatgcttta
     7201 tgaggataat aaacatcatg tgggagcggc aattcgtacg aaaacaggag aaatcatttc
     7261 ggcagtacat attgaagcgt atataggacg agtaactgtt tgtgcagaag ccattgcgat
     7321 tggtagtgca gtttcgaatg gacaaaagga ttttgacacg attgtagctg ttagacaccc
     7381 ttattctgac gaagtagata gaagtattcg agtggtaagt ccttgtggta tgtgtaggga
     7441 gttgatttca gactatgcac cagattgttt tgtgttaata gaaatgaatg gcaagttagt
     7501 caaaactacg attgaagaac tcattccact caaatatacc cgaaattaaa agtcgagatg
     7561 catcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     7621 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7681 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7741 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     7801 gatgcggtgg gctctatggc ttctgaggcg gaaagaacct gcagcccaag cttggcgtaa
     7861 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7921 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7981 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatctta
     8041 aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata
     8101 aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac
     8161 ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt
     8221 caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa
     8281 tctagtatcg tagattaatt aatccagctt ttgttccctt tagtgagggt taattgcgcg
     8341 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8401 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8461 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8521 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8941 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9181 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9301 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9481 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9541 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9601 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9661 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9721 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9781 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9841 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9901 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9961 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10021 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10081 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10141 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10201 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10261 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10321 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10381 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10441 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10501 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
    10561 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
    10621 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
    10681 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
    10741 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
    10801 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
    10861 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
    10921 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgt
//
