LOCUS       sequence-485631-       10233 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Expression of human ARID1A ∆IDR1 and ∆ARID (deletion of a.a. 2-1169)
            using piggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, 
            Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, 
            Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, 
            Cermakova K, Hodges HC
  TITLE     beta-catenin functions as a molecular adapter for disordered cBAF 
            interactions.
  JOURNAL   Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026.
  PUBMED    40695292
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026."
COMMENT     Sequence Label: pPB-CAG-ARID1A-deltaIDR1ARID-FLAG
FEATURES             Location/Qualifiers
     source          1..10233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     168..187
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        168..186
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   216..250
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        571..950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2203..2225
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             5679..5744
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5839..6388
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5983..6000)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6210..6229
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6859..6876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6865..7089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7136..7152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7160..7176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7184..7190,7191..7208,7209..7214))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7229..7250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7484..7546
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7587..7607)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7587..7605)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7626..7642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7650..7666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7674..7680,7681..7698,7699..7704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7719..7740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7857..7874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8028..8616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8108..8127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8787..9578,9579..9647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9410..9429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9648..9752)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9778..10233)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9915..9936)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10127..10146
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgctagac
      541 tcgagaagct tgatcggcca cgcggatctc gacattgatt attgactagt tattaatagt
      601 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      661 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      721 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      781 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      841 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      901 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
      961 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     1021 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggcgcgcgcc
     1081 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg ggggaggggc
     1141 ggcgcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     1201 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     1261 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     1321 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     1381 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     1441 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     1501 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     1561 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1621 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1681 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1741 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1801 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1861 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1921 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1981 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     2041 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     2101 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     2161 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     2221 tgaccggcgg ctctagccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     2281 cgacctctct ccccaggggg atccaccggg ctagcgccac catgcacagt cagatccccc
     2341 cattgccagg catgagcagg agcaattcag ttgggatcca ggatgccttt aatgatggaa
     2401 gtgactccac attccagaag cggaattcca tgactccaaa ccctgggtat cagcccagta
     2461 tgaatacctc tgacatgatg gggcgcatgt cctatgagcc aaataaggat ccttatggca
     2521 gcatgaggaa agctccaggg agtgatccct tcatgtcctc agggcagggc cccaacggcg
     2581 ggatgggtga cccctacagt cgtgctgccg gccctgggct aggaaatgtg gcgatgggac
     2641 cacgacagca ctatccctat ggaggtcctt atgacagagt gaggacggag cctggaatag
     2701 ggcctgaggg aaacatgagc actggggccc cacagccgaa tctcatgcct tccaacccag
     2761 actcggggat gtattctcct agccgctacc ccccgcagca gcagcagcag cagcagcaac
     2821 gacatgattc ctatggcaat cagttctcca cccaaggcac cccttctggc agccccttcc
     2881 ccagccagca gactacaatg tatcaacagc aacagcagaa ttacaagcgg ccaatggatg
     2941 gcacatatgg ccctcctgcc aagcggcacg aaggggagat gtacagcgtg ccatacagca
     3001 ctgggcaggg gcagcctcag cagcagcagt tgcccccagc ccagccccag cctgccagcc
     3061 agcaacaagc tgcccagcct tcccctcagc aagatgtata caaccagtat ggcaatgcct
     3121 atcctgccac tgccacagct gctactgagc gccgaccagc aggcggcccc cagaaccaat
     3181 ttccattcca gtttggccga gaccgtgtct ctgcaccccc tggcaccaat gcccagcaaa
     3241 acatgccacc acaaatgatg ggcggcccca tacaggcatc agctgaggtt gctcagcaag
     3301 gcaccatgtg gcaggggcgt aatgacatga cctataatta tgccaacagg cagagcacgg
     3361 gctctgcccc ccagggcccc gcctatcatg gcgtgaaccg aacagatgaa atgctgcaca
     3421 cagatcagag ggccaaccac gaaggctcgt ggccttccca tggcacacgc cagcccccat
     3481 atggtccctc tgcccctgtg ccccccatga caaggccccc tccatctaac taccagcccc
     3541 caccaagcat gcagaatcac attcctcagg tatccagccc tgctcccctg ccccggccaa
     3601 tggagaaccg cacctctcct agcaagtctc cattcctgca ctctgggatg aaaatgcaga
     3661 aggcaggtcc cccagtacct gcctcgcaca tagcacctgc ccctgtgcag ccccccatga
     3721 ttcggcggga tatcaccttc ccacctggct ctgttgaagc cacacagcct gtgttgaagc
     3781 agaggaggcg gctcacaatg aaagacattg gaaccccgga ggcatggcgg gtaatgatgt
     3841 ccctcaagtc tggtctcctg gcagagagca catgggcatt agataccatc aacatcctgc
     3901 tgtatgatga caacagcatc atgaccttca acctcagtca gctcccaggg ttgctagagc
     3961 tccttgtaga atatttccga cgatgcctga ttgagatctt tggcatttta aaggagtatg
     4021 aggtgggtga cccaggacag agaacgctac tggatcctgg gaggttcagc aaggtgtcta
     4081 gtccagctcc catggagggt ggggaagaag aagaagaact tctaggtcct aaactagaag
     4141 aggaagaaga agaggaagta gttgaaaatg atgaggagat agccttttca ggcaaggaca
     4201 agccagcttc agagaatagt gaggagaagc tgatcagtaa gtttgacaag cttccagtaa
     4261 agatcgtaca gaagaatgat ccatttgtgg tggactgctc agataagctt gggcgtgtgc
     4321 aggagtttga cagtggcctg ctgcactggc ggattggtgg gggggacacc actgagcata
     4381 tccagaccca cttcgagagc aagacagagc tgctgccttc ccggcctcac gcaccctgcc
     4441 caccagcccc tcggaagcat gtgacaacag cagagggtac accagggaca acagaccagg
     4501 aggggccccc acctgatgga cctccagaaa aacggatcac agccactatg gatgacatgt
     4561 tgtctactcg gtctagcacc ttgaccgagg atggagctaa gagttcagag gccatcaagg
     4621 agagcagcaa gtttccattt ggcattagcc cagcacagag ccaccggaac atcaagatcc
     4681 tagaggacga accccacagt aaggatgaga ccccactgtg tacccttctg gactggcagg
     4741 attctcttgc caagcgctgc gtctgtgtgt ccaataccat tcgaagcctg tcatttgtgc
     4801 caggcaatga ctttgagatg tccaaacacc cagggctgct gctcatcctg ggcaagctga
     4861 tcctgctgca ccacaagcac ccagaacgga agcaggcacc actaacttat gaaaaggagg
     4921 aggaacagga ccaaggggtg agctgcaaca aagtggagtg gtggtgggac tgcttggaga
     4981 tgctccggga aaacaccttg gttacactcg ccaacatctc ggggcagttg gacctatctc
     5041 cataccccga gagcatttgc ctgcctgtcc tggacggact cctacactgg gcagtttgcc
     5101 cttcagctga agcccaggac cccttttcca ccctgggccc caatgccgtc ctttccccgc
     5161 agagactggt cttggaaacc ctcagcaaac tcagcatcca ggacaacaat gtggacctga
     5221 ttctggccac accccccttc agccgcctgg agaagttgta tagcactatg gtgcgcttcc
     5281 tcagtgaccg aaagaacccg gtgtgccggg agatggctgt ggtactgctg gccaacctgg
     5341 ctcaggggga cagcctggca gctcgtgcca ttgcagtgca gaagggcagt atcggcaacc
     5401 tcctgggctt cctagaggac agccttgccg ccacacagtt ccagcagagc caggccagcc
     5461 tcctccacat gcagaaccca ccctttgagc caactagtgt ggacatgatg cggcgggctg
     5521 cccgcgcgct gcttgccttg gccaaggtgg acgagaacca ctcagagttt actctgtacg
     5581 aatcacggct gttggacatc tcggtatcac cgctgatgaa tagcctcgtg tcacaagtca
     5641 tttgtgatgt actgtttttg attggccagt ccaccggtga ctacaaagac catgacggtg
     5701 attataaaga tcatgatatc gattacaagg atgacgatga caagtgaacg cgttagtgcg
     5761 gatcctagtg cgaattctag tgcgtcgact ctagcaacgg tttccctcta gcgggatcaa
     5821 ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5881 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5941 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     6001 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     6061 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     6121 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     6181 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     6241 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     6301 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     6361 tggttttcct ttgaaaaaca cgataatacc atggtcatga aaacatttaa catttctcaa
     6421 caagatctag aattagtaga agtagcgaca gagaagatta caatgcttta tgaggataat
     6481 aaacatcatg tgggagcggc aattcgtacg aaaacaggag aaatcatttc ggcagtacat
     6541 attgaagcgt atataggacg agtaactgtt tgtgcagaag ccattgcgat tggtagtgca
     6601 gtttcgaatg gacaaaagga ttttgacacg attgtagctg ttagacaccc ttattctgac
     6661 gaagtagata gaagtattcg agtggtaagt ccttgtggta tgtgtaggga gttgatttca
     6721 gactatgcac cagattgttt tgtgttaata gaaatgaatg gcaagttagt caaaactacg
     6781 attgaagaac tcattccact caaatatacc cgaaattaaa agtcgagatg catcgatgat
     6841 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6901 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6961 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7021 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7081 gctctatggc ttctgaggcg gaaagaacct gcagcccaag cttggcgtaa tcatggtcat
     7141 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7201 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7261 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatctta aaagttttgt
     7321 tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata
     7381 aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta
     7441 ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg
     7501 catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa tctagtatcg
     7561 tagattaatt aatccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     7621 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7681 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7741 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7801 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7861 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7921 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8461 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8581 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8641 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8701 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8761 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8821 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8881 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8941 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9001 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9061 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9121 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9181 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9241 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9301 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9361 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9421 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9481 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9541 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9601 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9661 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9721 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     9781 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     9841 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     9901 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     9961 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
    10021 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
    10081 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
    10141 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
    10201 ccgccgcgct taatgcgccg ctacagggcg cgt
//
