LOCUS       sequence-485637-       12358 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  Expression of human ARID1A ∆IDR2 (deletion of a.a. 1170-1610) using 
            piggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12358)
  AUTHORS   Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, 
            Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, 
            Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, 
            Cermakova K, Hodges HC
  TITLE     beta-catenin functions as a molecular adapter for disordered cBAF 
            interactions.
  JOURNAL   Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026.
  PUBMED    40695292
REFERENCE   2  (bases 1 to 12358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026."
COMMENT     Sequence Label: pPB-CAG-ARID1A-deltaIDR2-FLAG
FEATURES             Location/Qualifiers
     source          1..12358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     168..187
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        168..186
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   216..250
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        571..950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        952..1206
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     primer_bind     2147..2169
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      2260..2269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             5046..5054
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7804..7869
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7964..8513
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8108..8125)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8335..8354
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(8984..9001)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8990..9214
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9261..9277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9285..9301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9309..9315,9316..9333,9334..9339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9354..9375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   9609..9671
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9712..9732)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9712..9730)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9751..9767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9775..9791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9799..9805,9806..9823,9824..9829))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9844..9865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9982..9999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10153..10741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10233..10252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10912..11703,11704..11772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11535..11554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11773..11877)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11903..12358)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12040..12061)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12252..12271
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      181 tatagggcga attggggcgc gccattctag attaacccta gaaagatagt ctgcgtaaaa
      241 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
      301 tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg
      361 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
      421 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
      481 tacgtgataa cttattatat atatattttc ttgttataga tatcaactag aatgctagac
      541 tcgagaagct tgatcggcca cgcggatctc gacattgatt attgactagt tattaatagt
      601 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      661 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      721 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      781 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      841 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      901 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
      961 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     1021 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggggggccgg
     1081 gggggggggg gggcgagggg gagagttgcg gcggcaacca atcagagcgg cgcgctccga
     1141 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     1201 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     1261 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     1321 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
     1381 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     1441 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     1501 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     1561 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     1621 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     1681 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     1741 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     1801 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
     1861 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     1921 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     1981 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     2041 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     2101 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     2161 cgtgtgaccg gcggctctag ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     2221 tcaccgacct ctctccccag ggggatccac cgggctagcg ccaccatggc cgcgcaggtc
     2281 gcccccgccg ccgccagcag cctgggcaac ccgccgccgc cgccgccctc ggagctgaag
     2341 aaagccgagc agcagcagcg ggaggaggcg gggggcgagg cggcggcggc ggcagcggcc
     2401 gagcgcgggg aaatgaaggc agccgccggg caggaaagcg agggccccgc cgtggggccg
     2461 ccgcagccgc tgggaaagga gctgcaggac ggggccgaga gcaatggggg tggcggcggc
     2521 ggcggagccg gcagcggcgg cgggcccggc gcggagccgg atctcaaaaa cagtaacgga
     2581 aacgctggcc ctaggcccgc cctgaacaat aacctcacgg agccgcccgg cggcggcggt
     2641 ggcggcagca gcgatggggt gggggcgcct cctcactcag ccgcggccgc cttgccgccc
     2701 ccagcctacg gcttcgggca accctacggc cggagcccgt ctgccgtcgc cgccgccgcg
     2761 gccgccgtct tccaccaaca acatggcgga caacaaagcc ctggcctggc agcgctgcag
     2821 agcggcggcg gcgggggcct ggagccctac gcggggcccc agcagaactc tcacgaccac
     2881 ggcttcccca accaccagta caactcctac taccccaacc gcagcgccta ccccccgccc
     2941 gccccggcct acgcgctgag ctccccgaga ggtggcactc cgggctccgg cgcggcggcg
     3001 gctgccggct ccaagccgcc tccctcctcc agcgcctccg cctcctcgtc gtcttcgtcc
     3061 ttcgctcagc agcgcttcgg ggccatgggg ggaggcggcc cctccgcggc cggcggggga
     3121 actccccagc ccaccgccac ccccaccctc aaccaactgc tcacgtcgcc cagctcggcc
     3181 cggggctacc agggctaccc cgggggcgac tacagtggcg ggccccagga cgggggcgcc
     3241 ggcaagggcc cggcggacat ggcctcgcag tgttgggggg ctgcggcggc ggcagctgcg
     3301 gcggcggccg cctcgggagg ggcccaacaa aggagccacc acgcgcccat gagccccggg
     3361 agcagcggcg gcggggggca gccgctcgcc cggacccctc agccatccag tccaatggat
     3421 cagatgggca agatgagacc tcagccatat ggcgggacta acccatactc gcagcaacag
     3481 ggacctccgt caggaccgca gcaaggacat gggtacccag ggcagccata cgggtcccag
     3541 accccgcagc ggtacccgat gaccatgcag ggccgggcgc agagtgccat gggcggcctc
     3601 tcttatacac agcagattcc tccttatgga caacaaggcc ccagcgggta tggtcaacag
     3661 ggccagactc catattacaa ccagcaaagt cctcaccctc agcagcagca gccaccctac
     3721 tcccagcaac caccgtccca gacccctcat gcccaacctt cgtatcagca gcagccacag
     3781 tctcaaccac cacagctcca gtcctctcag cctccatact cccagcagcc atcccagcct
     3841 ccacatcagc agtccccggc tccatacccc tcccagcagt cgacgacaca gcagcacccc
     3901 cagagccagc ccccctactc acagccacag gctcagtctc cttaccagca gcagcaacct
     3961 cagcagccag caccctcgac gctctcccag caggctgcgt atcctcagcc ccagtctcag
     4021 cagtcccagc aaactgccta ttcccagcag cgcttccctc caccgcagga gctatctcaa
     4081 gattcatttg ggtctcaggc atcctcagct cctagcatga caagctctaa aggagggcaa
     4141 gaagatatga acctgagcct tcagtcaaga cccagttctc tcccagactt gtcaggttct
     4201 atagatgacc tccccatggg gacagaagga gctctgagtc ctggagtgag cacatcaggg
     4261 atttccagca gccaaggaga gcagagtaat ccagctcagt ctcctttctc tcctcatacc
     4321 tcccctcacc tgcctggcat ccgaggccct tccccgtccc ctgttggctc tcccgccagt
     4381 gttgctcagt ctcgctcagg accactctcg cctgctgcag tgccaggcaa ccagatgcca
     4441 cctcggccac ccagtggcca gtcggacagc atcatgcatc cttccatgaa ccaatcaagc
     4501 attgcccaag atcgaggtta tatgcagagg aacccccaga tgccccagta cagttccccc
     4561 cagcccggct cagccttatc tccgcgtcag ccttccggag gacagataca cacaggcatg
     4621 ggctcctacc agcagaactc catggggagc tatggtcccc aggggggtca gtatggccca
     4681 caaggtggct accccaggca gccaaactat aatgccttgc ccaatgccaa ctaccccagt
     4741 gcaggcatgg ctggaggcat aaaccccatg ggtgccggag gtcaaatgca tggacagcct
     4801 ggcatcccac cttatggcac actccctcca gggaggatga gtcacgcctc catgggcaac
     4861 cggccttatg gccctaacat ggccaatatg ccacctcagg ttgggtcagg gatgtgtccc
     4921 ccaccagggg gcatgaaccg gaaaacccaa gaaactgctg tcgccatgca tgttgctgcc
     4981 aactctatcc aaaacaggcc gccaggctac cccaatatga atcaaggggg catgatggga
     5041 actggacctc cttatggaca agggattaat agtatggctg gcatgatcaa ccctcaggga
     5101 cccccatatt ccatgggtgg aaccatggcc aacaattctg cagggatggc agccagccca
     5161 gagatgatgg gccttgggga tgtaaagtta actccagcca ccaaaatgaa caacaaggca
     5221 gatgggacac ccaagacaga atccaaatcc aagaaatcca gttcttctac tacaaccaat
     5281 gagaagatca ccaagttgta tgagctgggt ggtgagcctg agaggaagat gtgggtggac
     5341 cgttatctgg ccttcactga ggagaaggcc atgggcatga caaatctgcc tgctgtgggt
     5401 aggaaacctc tggacctcta tcgcctctat gtgtctgtga aggagattgg tggattgact
     5461 caggtcaaca agaacaaaaa atggcgggaa cttgcaacca acctcaatgt gggcacatca
     5521 agcagtgctg ccagctcctt gaaaaagcag tatatccagt gtctctatgc ctttgaatgc
     5581 aagattgaac ggggagaaga ccctccccca gacatctttg cagctgctga ttccaagaag
     5641 tcccagccca agatccagcc tccctctcct gcgggatcag gatctatgca ggggccccag
     5701 actccccagt caaccagcag ttccatggca gaaggaggag acttaaagcc accaactcca
     5761 gcatccacac caatgaaaat gcagaaggca ggtcccccag tacctgcctc gcacatagca
     5821 cctgcccctg tgcagccccc catgattcgg cgggatatca ccttcccacc tggctctgtt
     5881 gaagccacac agcctgtgtt gaagcagagg aggcggctca caatgaaaga cattggaacc
     5941 ccggaggcat ggcgggtaat gatgtccctc aagtctggtc tcctggcaga gagcacatgg
     6001 gcattagata ccatcaacat cctgctgtat gatgacaaca gcatcatgac cttcaacctc
     6061 agtcagctcc cagggttgct agagctcctt gtagaatatt tccgacgatg cctgattgag
     6121 atctttggca ttttaaagga gtatgaggtg ggtgacccag gacagagaac gctactggat
     6181 cctgggaggt tcagcaaggt gtctagtcca gctcccatgg agggtgggga agaagaagaa
     6241 gaacttctag gtcctaaact agaagaggaa gaagaagagg aagtagttga aaatgatgag
     6301 gagatagcct tttcaggcaa ggacaagcca gcttcagaga atagtgagga gaagctgatc
     6361 agtaagtttg acaagcttcc agtaaagatc gtacagaaga atgatccatt tgtggtggac
     6421 tgctcagata agcttgggcg tgtgcaggag tttgacagtg gcctgctgca ctggcggatt
     6481 ggtggggggg acaccactga gcatatccag acccacttcg agagcaagac agagctgctg
     6541 ccttcccggc ctcacgcacc ctgcccacca gcccctcgga agcatgtgac aacagcagag
     6601 ggtacaccag ggacaacaga ccaggagggg cccccacctg atggacctcc agaaaaacgg
     6661 atcacagcca ctatggatga catgttgtct actcggtcta gcaccttgac cgaggatgga
     6721 gctaagagtt cagaggccat caaggagagc agcaagtttc catttggcat tagcccagca
     6781 cagagccacc ggaacatcaa gatcctagag gacgaacccc acagtaagga tgagacccca
     6841 ctgtgtaccc ttctggactg gcaggattct cttgccaagc gctgcgtctg tgtgtccaat
     6901 accattcgaa gcctgtcatt tgtgccaggc aatgactttg agatgtccaa acacccaggg
     6961 ctgctgctca tcctgggcaa gctgatcctg ctgcaccaca agcacccaga acggaagcag
     7021 gcaccactaa cttatgaaaa ggaggaggaa caggaccaag gggtgagctg caacaaagtg
     7081 gagtggtggt gggactgctt ggagatgctc cgggaaaaca ccttggttac actcgccaac
     7141 atctcggggc agttggacct atctccatac cccgagagca tttgcctgcc tgtcctggac
     7201 ggactcctac actgggcagt ttgcccttca gctgaagccc aggacccctt ttccaccctg
     7261 ggccccaatg ccgtcctttc cccgcagaga ctggtcttgg aaaccctcag caaactcagc
     7321 atccaggaca acaatgtgga cctgattctg gccacacccc ccttcagccg cctggagaag
     7381 ttgtatagca ctatggtgcg cttcctcagt gaccgaaaga acccggtgtg ccgggagatg
     7441 gctgtggtac tgctggccaa cctggctcag ggggacagcc tggcagctcg tgccattgca
     7501 gtgcagaagg gcagtatcgg caacctcctg ggcttcctag aggacagcct tgccgccaca
     7561 cagttccagc agagccaggc cagcctcctc cacatgcaga acccaccctt tgagccaact
     7621 agtgtggaca tgatgcggcg ggctgcccgc gcgctgcttg ccttggccaa ggtggacgag
     7681 aaccactcag agtttactct gtacgaatca cggctgttgg acatctcggt atcaccgctg
     7741 atgaatagcc tcgtgtcaca agtcatttgt gatgtactgt ttttgattgg ccagtccacc
     7801 ggtgactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
     7861 gatgacaagt gaacgcgtta gtgcggatcc tagtgcgaat tctagtgcgt cgactctagc
     7921 aacggtttcc ctctagcggg atcaattccg cccccccccc ctaacgttac tggccgaagc
     7981 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     8041 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     8101 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     8161 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     8221 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     8281 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     8341 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     8401 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     8461 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggt
     8521 catgaaaaca tttaacattt ctcaacaaga tctagaatta gtagaagtag cgacagagaa
     8581 gattacaatg ctttatgagg ataataaaca tcatgtggga gcggcaattc gtacgaaaac
     8641 aggagaaatc atttcggcag tacatattga agcgtatata ggacgagtaa ctgtttgtgc
     8701 agaagccatt gcgattggta gtgcagtttc gaatggacaa aaggattttg acacgattgt
     8761 agctgttaga cacccttatt ctgacgaagt agatagaagt attcgagtgg taagtccttg
     8821 tggtatgtgt agggagttga tttcagacta tgcaccagat tgttttgtgt taatagaaat
     8881 gaatggcaag ttagtcaaaa ctacgattga agaactcatt ccactcaaat atacccgaaa
     8941 ttaaaagtcg agatgcatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct
     9001 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     9061 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     9121 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     9181 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc
     9241 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9421 tgccagcgga tcttaaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt
     9481 tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg
     9541 taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg
     9601 ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta
     9661 tctttctagg gttaatctag tatcgtagat taattaatcc agcttttgtt ccctttagtg
     9721 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9781 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9841 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9901 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9961 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10021 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10081 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10141 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10201 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10261 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10321 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10381 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10441 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10501 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10561 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10621 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10681 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10741 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10801 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10861 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10921 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10981 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11041 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11101 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11161 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11221 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11281 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11341 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11401 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11461 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11521 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11581 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11641 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11701 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11761 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11821 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11881 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
    11941 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    12001 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    12061 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    12121 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    12181 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    12241 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    12301 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgt
//
