LOCUS       sequence-485750-        5583 bp    DNA     linear   UNA 11-SEP-2025
DEFINITION  L-arabinose inducible E.coli cascade operon inserted in pCDF vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5583)
  TITLE     Main plasmids in Region specific tool for continuous directed 
            evolution (RESPECTevo)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDF-cascade
FEATURES             Location/Qualifiers
     source          1..5583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..879)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        906..1190
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1113..1132
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat agaaatgcag
     1201 gagaaatgtc ggagcatatg aaggagaaca aatgaatttg cttattgata actggatccc
     1261 tgtacgcccg cgaaacgggg ggaaagtcca aatcataaat ctgcaatcgc tatactgcag
     1321 tagagatcag tggcgattaa gtttgccccg tgacgatatg gaactggccg ctttagcact
     1381 gctggtttgc attgggcaaa ttatcgcccc ggcaaaagat gacgttgaat ttcgacatcg
     1441 cataatgaat ccgctcactg aagatgagtt tcaacaactc atcgcgccgt ggatagatat
     1501 gttctacctt aatcacgcag aacatccctt tatgcagacc aaaggtgtca aagcaaatga
     1561 tgtgactcca atggaaaaac tgttggctgg ggtaagcggc gcgacgaatt gtgcatttgt
     1621 caatcaaccg gggcagggtg aagcattatg tggtggatgc actgcgattg cgttattcaa
     1681 ccaggcgaat caggcaccag gttttggtgg tggttttaaa agcggtttac gtggaggaac
     1741 acctgtaaca acgttcgtac gtgggatcga tcttcgttca acggtgttac tcaatgtcct
     1801 cacattacct cgtcttcaaa aacaatttcc taatgaatca catacggaaa accaacctac
     1861 ctggattaaa cctatcaagt ccaatgagtc tatacctgct tcgtcaattg ggtttgtccg
     1921 tggtctattc tggcaaccag cgcatattga attatgcgat cccattggga ttggtaaatg
     1981 ttcttgctgt ggacaggaaa gcaatttgcg ttataccggt tttcttaagg aaaaatttac
     2041 ctttacagtt aatgggctat ggccccatcc gcattcccct tgtctggtaa cagtcaagaa
     2101 aggggaggtt gaggaaaaat ttcttgcttt caccacctcc gcaccatcat ggacacaaat
     2161 cagccgagtt gtggtagata agattattca aaatgaaaat ggaaatcgcg tggcggcggt
     2221 tgtgaatcaa ttcagaaata ttgcgccgca aagtcctctt gaattgatta tggggggata
     2281 tcgtaataat caagcatcta ttcttgaacg gcgtcatgat gtgttgatgt ttaatcaggg
     2341 gtggcaacaa tacggcaatg tgataaacga aatagtgact gttggtttgg gatataaaac
     2401 agccttacgc aaggcgttat atacctttgc agaagggttt aaaaataaag acttcaaagg
     2461 ggccggagtc tctgttcatg agactgcaga aaggcatttc tatcgacaga gtgaattatt
     2521 aattcccgat gtactggcga atgttaattt ttcccaggct gatgaggtaa tagctgattt
     2581 acgagacaaa cttcatcaat tgtgtgaaat gctatttaat caatctgtag ctccctatgc
     2641 acatcatcct aaattaataa gcacattagc gcttgcccgc gccacgctat acaaacattt
     2701 acgggagtta aaaccgcaag gagggccatc aaatggctga tgaaattgat gcaatggctt
     2761 tatatcgagc ctggcaacaa ctggataatg gatcatgtgc gcaaattaga cgtgtttcag
     2821 aacctgatga attacgcgat atccctgcgt tttataggct ggtgcaacct tttggttggg
     2881 aaaacccacg tcaccagcag gctcttttgc gcatggtgtt ttgcctgagc gcaggaaaga
     2941 atgtcatccg acatcaggac aaaaaatcgg agcaaacaac aggtatctcg ttgggaagag
     3001 ctttagccaa tagtggaaga attaacgagc gccgtatctt tcaattaatt cgggctgaca
     3061 gaacagccga tatggtccag ttacgtcgat tacttactca cgccgaaccc gtacttgact
     3121 ggccattaat ggccaggatg ttgacctggt ggggaaagcg cgaacgccag caacttctgg
     3181 aagattttgt attgaccaca aacaaaaatg cgtaaggaaa cctttctatg tctaacttta
     3241 tcaatattca tgttctgatc tctcacagcc cttcatgtct gaaccgcgac gatatgaaca
     3301 tgcagaaaga cgctattttc ggcggcaaaa gacgagtaag aatttcaagt caaagcctta
     3361 aacgtgcgat gcgtaaaagt ggttattacg cacaaaatat tggtgaatcc agtctcagaa
     3421 ccattcatct tgcacaatta cgtgatgttc ttcggcaaaa acttggtgaa cgttttgacc
     3481 aaaaaatcat cgataagaca ttagcgctgc tctccggtaa atcagttgat gaagccgaaa
     3541 agatttctgc cgatgcggtt actccctggg ttgtgggaga aatagcctgg ttctgtgagc
     3601 aggttgcaaa agcagaggct gataatctgg atgataaaaa gctgctcaaa gttcttaagg
     3661 aagatattgc cgccatacgt gtgaatttac agcagggtgt tgatattgcg cttagtggaa
     3721 gaatggcaac cagcggcatg atgactgagt tgggaaaagt tgatggtgca atgtccattg
     3781 cgcatgcgat cactactcat caggttgatt ctgatattga ctggttcacc gctgtagatg
     3841 atttacagga acaaggttct gcacatctgg gaactcagga attttcatcg ggtgtttttt
     3901 atcgttatgc caacattaac ctcgctcaac ttcaggaaaa tttaggtggt gcctccaggg
     3961 agcaggctct ggaaattgca acccatgttg ttcatatgct ggcaacagag gtccctggag
     4021 caaaacagcg tacttatgcc gcttttaacc ctgcggatat ggtaatggtt aatttctccg
     4081 atatgccact ttctatggca aatgcttttg aaaaagcggt taaagcgaaa gatggctttt
     4141 tgcaaccgtc tatacaggcg tttaatcaat attgggatcg cgttgccaat ggatatggtc
     4201 tgaacggagc tgctgcgcaa ttcagcttat ctgatgtaga cccaattact gctcaagtta
     4261 aacaaatgcc tactttagaa cagttaaaat cctgggttcg taataatggc gaggcgtgaa
     4321 catgagatct tatttgatct tgcggcttgc tgggccaatg caagcctggg ggcagccgac
     4381 ctttgaagga acgcgaccta ccggaagatt tccgacccga agcgggttat tagggctact
     4441 cggggcttgt cttgggatcc aacgtgatga tacttcttca ttacaggcgt tatcagagag
     4501 tgtgcaattt gcagtgcgct gcgatgaact cattcttgac gatcgtcgtg tgtctgtaac
     4561 ggggttgcgt gattaccata cagtccttgg agcgcgagaa gattaccgtg gtttgaaaag
     4621 tcatgaaacg attcaaacat ggcgcgaata tttatgtgat gcctccttta ccgtcgctct
     4681 ctggttaaca ccccatgcaa cgatggttat ctcagaactt gaaaaagcag tattaaagcc
     4741 tcggtataca ccttacctgg ggcggagaag ttgcccacta acacacccgc tttttttggg
     4801 gacatgtcag gcatcggatc ctcagaaggc gctattaaat tatgagcccg ttggcggcga
     4861 tatatatagt gaggaatcag ttacagggca tcatttaaaa tttacggcgc gcgacgaacc
     4921 gatgatcacc ttgcctcgac aatttgcttc ccgagaatgg tatgtgatta aaggaggtat
     4981 ggatgtatct cagtaaagtc atcattgcca gggcctggag cagggatctt taccaacttc
     5041 accagggatt atggcattta tttccaaaca gaccggatgc tgctcgtgat tttctttttc
     5101 atgttgagaa gcgaaacaca ccagaaggct gtcatgtttt attgcagtca gcgcaaatgc
     5161 ctgtttcaac tgccgttgcg acagtcatta aaactaaaca ggttgaattt caacttcagg
     5221 ttggtgttcc actctatttt cggcttcggg caaatccgat caaaactatt ctcgacaatc
     5281 aaaagcgcct ggacagtaaa gggaatatta aacgctgtcg ggttccgtta ataaaagaag
     5341 cagaacaaat cgcgtggttg caacgtaaat tgggcaatgc ggcgcgcgtt gaagatgtgc
     5401 atcccatatc ggaacggcca cagtattttt ctggtgatgg taaaagtgga aagatccaaa
     5461 cggtttgctt tgaaggtgtg ctcaccatca acgacgcgcc agcgttaata gatcttgtac
     5521 agcaaggtat tgggccagct aaatcgatgg gatgtggctt gctatctttg gctccactgt
     5581 gat
//
