LOCUS       sequence-485799-        8029 bp    DNA     circular SYN 11-SEP-2025
DEFINITION  Bidirectional AAV transfer plasmid expressing hSyn-tTA and 
            TRE-FLEX-mNeonGreen-NWS in the other direction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   n/a
  TITLE     E11 Bio PRISM Collection Plasmids 2025
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pAAV-BD-hSynTet-TRE-DIO-mNeonGreen-NWS
FEATURES             Location/Qualifiers
     source          1..8029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(171..204)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      800..809
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1061..1070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1077..1110
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    1161..1194
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1219..1807
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(1690..1701)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    1839..2315
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2038..2057)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2355..2495
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2355..2484
                     /label=AAV2 ITR
     rep_origin      2570..3025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2657..2676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2867..2888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3042..3061)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3161..3183
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3221..3239)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3307..3411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3412..3480,3481..4272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3630..3649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4443..5031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4932..4951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5093..5222
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    5232..5353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5269..5288)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5323..5342
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(5377..5385,5386..5421,5422..5424,5425..5460,
                     5461..5463,5464..5499,5500..5505,5506..6123))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    6579..6597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6616..6634
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6653..6671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6690..6708
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6727..6745
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6764..6782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6801..6819
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6838..6856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6878..6902
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(7001..7034)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(7085..7118)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(7225..7248)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     7702..7723
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7877..7900
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 gatgaaatgt actatgctga ctatttagga ccaccgtaga gatgctttat ttcccctctg
       61 atggcttgga atgctctttt gctctgggca aaagttctca atgccagtag gacaggtgta
      121 atattgtcta cctcatrymk kkaaagctay tagcratatg aaacctctta ataacttcgt
      181 atagcataca ttatacgaag ttatggcgcg cctcacttgt ccaaggaatt cttactttga
      241 ttatcgataa gcttgacctg gkkmakrkkt saccksmysm mtacscacca gtcctactga
      301 agaattctta ywtgcygtcn nnnntcsakc wgtckmrgst ccwsctsgty ctcgctgccg
      361 cccagctcgt ccatgcccat cacatcggta aaggcctttt gccactcctt gaagttgagc
      421 tcggtcttgg agtgcttgag ctccgtctta cggaacacgt acatcggctg gttcttcaga
      481 tagttagccg ccattggctt ggcaaaggtg taggtggtcc gcgcagtgct ccggtagcgc
      541 ttgccatttc cagtggtgta actccactta aaggtactga tgatggtttt gtcgttgggg
      601 taagtcttct tcgacctgca ccagtccgca gcggtcagcg agttggtcat cacaggaccg
      661 tcagcaggga aaccagtccc cttcacctgg gcctctcctt tgatgtggct tccctcgtag
      721 gtgtagcggt agttaacagt aagggaggca ccatcttcaa actgcattgt gcgatggact
      781 tggtatccgg agccatctac catggcggcc tggaaaggcg acatcccgtc agggtagggc
      841 aggtactgat ggaagccata cccgatatga gggaccagaa tccaggggga gaactggagg
      901 tcacccttgg tggacttcag gtttaactcc tcataaccat catttggatt gccggtgccc
      961 tgacccacca tgtcaaagtc cacaccgttg atggagccaa agatgtgtaa ctcatgtgtc
     1021 gctgggagag aggccatgtt atcctcctcg cccttgctca ccatggtggc gctagcataa
     1081 cttcgtataa agtatcctat acgaagttat ttgccttaac ccagaaatta tcactgttat
     1141 tctttagaat ggtgcaaaga ataacttcgt ataatgtatg ctatacgaag ttatgaattc
     1201 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     1261 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     1321 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     1381 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     1441 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     1501 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     1561 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     1621 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     1681 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     1741 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     1801 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     1861 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     1921 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     1981 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     2041 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     2101 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     2161 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     2221 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     2281 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     2341 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     2401 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     2461 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     2521 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     2581 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2641 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2701 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2761 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     2821 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2881 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     2941 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3001 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     3061 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3121 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3181 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     3241 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3301 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3361 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3421 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3481 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3541 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3601 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     3661 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3721 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3781 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3841 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3901 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     3961 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4021 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4081 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4141 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4201 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4261 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4321 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4381 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4441 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4501 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4561 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     4621 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4681 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4741 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4801 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4861 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4921 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4981 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5041 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtcctgcagg
     5101 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc
     5161 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc
     5221 ctgcggccgc aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5281 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5341 tcaatgtatc ttatcatgtc tgagatcctt acttagttac ccggggagca tgtcaaggtc
     5401 aaaatcgtca agagcgtcag caggcagcat atcaaggtca aagtcgtcaa gggcatcggc
     5461 tgggagcatg tctaagtcaa aatcgtcaag ggcgtcggcc ggcccgccgc tttcgcactt
     5521 tagctgtttc tccaggccac atatgattag ttccaggccg aaaaggaagg caggttcggc
     5581 tccctgatgg tcgaacagct caattgcttg tctcagaagt gggggcatag aatcggtggt
     5641 aggtgtctct ctttcctctt ttgctacttg atgctcctga tcctccaata cgcagcccag
     5701 tgtaaagtgg cccacggcgg acagagcgta cagtgcgttc tccagggaga agccttgctg
     5761 acacaggaac gcgagctgat tttccagggt ttcgtactgt ttctctgttg ggcgggtgcc
     5821 gagatgcact ttagccccgt cgcgatgtga gaggagagca cagcggaatg acttggcgtt
     5881 gttccgcaga aagtcttgcc atgactcgcc ttccaggggg cagaagtggg tatgatgcct
     5941 gtccagcatc tcgattgcca gggcatcgag cagggcccgc ttgttcttca cgtgccagta
     6001 cagggtaggc tgctcaactc ccagcttttg agcgagtttc cttgtcgtca ggccttcgat
     6061 accgacttca ttgagtaatt ccagagcaga gtttatgact ttgctcttgt ccagtctaga
     6121 catggtgaat tcgatatcaa gcttatcgat accgtccgcc gcagcgcaga tggtcgcgcc
     6181 cgtgcccccc tatctcgcgc ctcgcgtggt gcggtccggc tgggccggcg gcggcgcgga
     6241 cgcgaccaag gtggccggga aggggagttt gcgggggacc ggcgagtgac gtcagcgcgc
     6301 cttcagtgct gaggcggcgg tggcgcgcgc cgccaggcgg gggcgaaggc actgtccgcg
     6361 gtgctgaagc tggcagtgcg cacgcgcctc gccgcatcct gtttcccctc cccctctctg
     6421 ataggggatg cgcaatttgg ggaatggggg ttgggtgctt gtccagtggg tcggggtcgg
     6481 tcgtcaggta ggcaccccca ccccgcctca tcctggtcct aaaacccact tgcactcata
     6541 cgcagggccc tctgcagtat caagtgccac ctgacgtctc cctatcagtg atagagaagt
     6601 cgacacgtct cgagctccct atcagtgata gagaaggtac gtctagaacg tctccctatc
     6661 agtgatagag aagtcgacac gtctcgagct ccctatcagt gatagagaag gtacgtctag
     6721 aacgtctccc tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga
     6781 gaaggtacgt ctagaacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc
     6841 ctatcagtga tagagaagct accccctata taagcagagc tcgtttagtg aaccgtcaga
     6901 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     6961 gcctggatcc ggtaccggat cctctagagt cgactccgga ataacttcgt ataggatact
     7021 ttatacgaag ttatgcagaa tggtagctgg attgtagctg ctattagcaa tatgaaacct
     7081 cttaataact tcgtatagca tacattatac gaagttatgg cgcgcctcac ttgtccaagg
     7141 aattcttact ttgattatcg ataagcttga cctggtctaa gacgcgggtt ggtacggaat
     7201 accagtccta ctgaagaatt cttacttctc gaactgaggg tggctccagg cgctgccgcc
     7261 cagctcgtcc atgcccatca catcggtaaa ggccttttgc cactccttga agttgagctc
     7321 ggtcttggag tgcttgagct ccgtcttacg gaacacgtac atcggctggt tcttcagata
     7381 gttagccgcc attggcttgg caaaggtgta ggtggtccgc gcagtgctcc ggtagcgctt
     7441 gccatttcca gtggtgtaac tccacttaaa ggtactgatg atggttttgt cgttggggta
     7501 agtcttcttc gacctgcacc agtccgcagc ggtcagcgag ttggtcatca caggaccgtc
     7561 agcagggaaa ccagtcccct tcacctgggc ctctcctttg atgaacaccc ccatcggcga
     7621 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     7681 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     7741 tctcggcatg gacgagctgt acaagggrgk mkmyggcgsk tcwmmktksw macacccyya
     7801 kywcgmgmaa kgtgrtyccg gcggwwgyma ctggmgccat cctcaattcg aaaaaggggg
     7861 aagcgggggc agcaattgga gccacccaca atttgaaaaa taagaattcg atatcaagct
     7921 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7981 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtat
//
