LOCUS       sequence-485802-       11103 bp    DNA     circular SYN 11-SEP-2025
DEFINITION  2nd gen bicistronic lentiviral vector allows for simultaneous 
            expression of a transgene and EGFP marker to facilitate tracking of 
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11103)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI
FEATURES             Location/Qualifiers
     source          1..11103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     182..202
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(272..289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(443..1031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(523..542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1202..1993,1994..2062))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1825..1844
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2063..2167)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2235..2253
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2291..2313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2413..2432
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2646..2665
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2955..2973
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2955..2972
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             2998..3178
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    3198..3231
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3375..3963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3481..3492
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3890..3910
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     3976..3992
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3977..3993
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(4072..4782,4783..4785,4786..4788))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4072..4782,4783..4785,4786..4788))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4114..4135)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4463..4482
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4722..4743
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    4791..5342
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4793..5360
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4952..4971)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5181..5198
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     misc_feature    5409..5526
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(5574..6752)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5583..6521
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5606..5626)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(7135..7179)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7364..7597
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8090..8215
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8262..8895
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9063..9197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9087..9106)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9141..9160
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9622..9642)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9772..9837
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(join(10971..11103,1..197))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10985..11103,1..17)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
       61 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
      121 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
      181 caccctaact gacacacatt ccacagctgc attaatgaat cggccaacgc gcggggagag
      241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      841 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2161 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2221 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     2281 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2341 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     2401 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattcg acgctctccc
     2461 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     2521 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     2581 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     2641 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     2701 gccacgatgc gtccggcgta gaggatctgg ctagcgatga ccctgctgat tggttcgctg
     2761 accatttccg ggtgcgggac ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg
     2821 actctgacgg cagtttacga gagagatgat agggtctgct tcagtaagcc agatgctaca
     2881 caattaggct tgtacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt
     2941 atcatacaca tacgatttag gtgacactat agaatacacg gaattaattc tagatgctgc
     3001 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     3061 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     3121 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
     3181 tacaagcaaa aagcagcata acttcgtata atgtatgcta tacgaagtta ttcccatggt
     3241 gaattgatcc catcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct
     3301 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtaccgag
     3361 ctcgaattcc cgatgcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     3421 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     3481 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     3541 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
     3601 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     3661 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     3721 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     3781 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     3841 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     3901 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     3961 attatcggaa ttccctcgag gtcgacggta tcgcgataag cttgatatcg aattcgaagc
     4021 ttgagctcga ctagctagct agctactagc tagtcgagat ctgagtccgg acttgtacag
     4081 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     4141 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     4201 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     4261 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     4321 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     4381 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     4441 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     4501 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     4561 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     4621 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     4681 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     4741 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tattatcatc
     4801 gtgtttttca aaggaaaacc acgtccccgt ggttcggggg gcctagacgt tttttaacct
     4861 cgactaaaca catgtaaagc atgtgcaccg aggccccaga tcagatccca tacaatgggg
     4921 taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc atttgactct
     4981 ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg caggtgtatc
     5041 ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt tccgctgcct
     5101 gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt
     5161 cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc taggaatgct
     5221 cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg gcaatatggt
     5281 ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc ggccagtaac
     5341 gttagggggg ggggggcgga attcctgcag cccgtagttt aaacctcgag gctagtctcg
     5401 tgatcgataa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
     5461 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
     5521 ttaaaagtta accgataccg tcgagattaa ttaaatttaa atgtcgaaat tcctcacgac
     5581 acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat gaaccaagat
     5641 ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc tggcctaact
     5701 tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc cccccaacct
     5761 aaagacgacg tactccaaaa gctcgagaac taatcgaggt gcctggacgg cgcccggtac
     5821 tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct ttgtgtgggt
     5881 gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg
     5941 ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag ccttgccgcc
     6001 cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg gccagcttga
     6061 gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg
     6121 cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc
     6181 agatcttggc ccgcatttac aagactatct tgccagaaaa aaagcgtcgc agcaggtcat
     6241 caaaaatttt aaatggctag agacttatcg aaagcagcga gacaggcgcg aaggtgccac
     6301 cagattcgca cgcggcggcc ccagcgccca ggccaggcct caactcaagc acgaggcgaa
     6361 ggggctcctt aagcgcaagg cctcgaactc tcccacccac ttccaacccg aagctcggga
     6421 tcaagaatca cgtactgcag ccaggtggaa gtaattcaag gcacgcaagg gccataaccc
     6481 gtaaagaggc caggcccgcg ggaaccacac acggcactta cctgtgttct ggcggcaaac
     6541 ccgttgcgaa aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc
     6601 tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt
     6661 ctctaggcac cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat
     6721 gtgcgctctg cccactgacg ggcaccggag ccaattccca ctcctttcaa gacctagaag
     6781 gtccattagc tgcaaagatt cctctctgtt taaaacttta tccatctttg caaagcttat
     6841 cgtcgacatc gatggtcgag gctagtctcg tgatcgatac cgtcgagatc cgttcactaa
     6901 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc
     6961 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
     7021 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
     7081 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
     7141 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
     7201 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
     7261 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
     7321 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
     7381 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
     7441 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
     7501 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
     7561 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
     7621 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
     7681 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa
     7741 gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
     7801 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
     7861 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
     7921 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
     7981 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     8041 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     8101 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     8161 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     8221 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     8281 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     8341 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     8401 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag
     8461 ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc
     8521 ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt
     8581 actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact
     8641 ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg
     8701 tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc
     8761 accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag
     8821 ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt ctttgggagt
     8881 gaattagccc ttccaactac taagtttgta gtacatattt aacaaataca atttctttaa
     8941 aatgaaaata attcagagga atcacaggtt tagagtaaat gaaaccacag gtaattggca
     9001 gtggtaatag ggtatggggt gggaagtttg ggatgatttt ggttagcttg agttatccag
     9061 ttgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     9121 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     9181 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     9241 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     9301 atctctagtc aaggcactat acatcaaata ttccttatta acccctttac aaattaaaaa
     9361 gctaaaggta cacaattttt gagcatagtt attaatagca gacactctat gcctgtgtgg
     9421 agtaagaaaa aacagtatgt tatgattata actgttatgc ctacttataa aggttacaga
     9481 atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc
     9541 aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga
     9601 aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga
     9661 gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc
     9721 attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa tctaaaatac
     9781 acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt
     9841 agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga agtaaggttc
     9901 cttcacaaag atccggggcc cactcataaa tccagttgcc gccacggtag ccaatcaccg
     9961 tatcgtataa atcatcgtcg gtacgttcgg catcgctcat cacaatacgt gcctggacgt
    10021 cgaggatttc gcgtgggtca atgccgcgcc agatccacat cagacggtta atcatgcgat
    10081 accagtgagg gatggtttta ccatcaaggg ccgactgcac aggcggttgt gcgccgtgat
    10141 taaagcggcg gactagcgtc gaggtttcag gatgtttaaa gcggggtttg aacagggttt
    10201 cgctcaggtt tgcctgtgtc atggatgcag cctccagaat acttactgga aactattgta
    10261 acccgcctga agttaaaaag aacaacgccc ggcagtgcca ggcgttgaaa agattagcga
    10321 ccggagattg gcgggacgaa tacgacgccc atatcccacg gctgttcaat ccaggtatct
    10381 tgcgggatat caacaacata gtcatcaacc agcggacgac cagccggttt tgcgaagatg
    10441 gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa ccgcagtacc accggtatcc
    10501 accaggtcat caataacgat gaagccttcg ccatcgcctt ctgcgcgttt cagcacttta
    10561 agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa cggtatcgac atgacgaata
    10621 cccagttcac gcgccagtaa cgcacccggt acagaccgcc acggcttacg gcaataatgc
    10681 ctttccattg ttcagaaggc atcagtcggc ttgcgagttt acgtgcatgg atctgcaaca
    10741 tgtcccaggt gacgatgtat ttttcgctca tgtgaagtgt cccagcctgt ttatctacgg
    10801 cttaaaaagt gttcgagggg aaaataggtt gcgcgagatt atagagatct ggcgcactaa
    10861 aaaccagtat ttcacatgag tccgcgtctt tttacgcact gcctctccct gacgcgggat
    10921 aaagtggtat tctcaaacat atctcgcaag cctgtcttgt gtccaagctt tttgcaaaag
    10981 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
    11041 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
    11101 ggc
//
