LOCUS       sequence-485833-        9775 bp    DNA     circular SYN 12-SEP-2025
DEFINITION  Inducible FLAG-tagged mouse cGAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9775)
  AUTHORS   Wu Y, Song K, Hao W, Li J, Wang L, Li S
  TITLE     Nuclear soluble cGAS senses double-stranded DNA virus infection.
  JOURNAL   Commun Biol. 2022 May 10;5(1):433. doi: 10.1038/s42003-022-03400-1.
  PUBMED    35538147
REFERENCE   2  (bases 1 to 9775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 12, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-05-10"; volume: "5(1):433. doi"; pages: " 
            10.1038/s42003-022-03400-1"
COMMENT     Sequence Label: pCW57-mcGAS
FEATURES             Location/Qualifiers
     source          1..9775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..351
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     324..348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      368..377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1916..1987
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             2009..2065
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        2093..2603
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2522..2540
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2621..3643
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2621..3643
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3659..3712
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3713..4330,4331..4336,4337..4372,4373..4375,4376..4411,
                     4412..4414,4415..4450,4451..4459)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4499..4519)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4917..4928)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5133..5366
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5438..5559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5475..5494)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5529..5548
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5599..5734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5734..5805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5806..5874,5875..6666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6024..6043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6837..7425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7326..7345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7579..7596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7713..7734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7749..7754,7755..7772,7773..7779)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7787..7803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7792..7814
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7811..7827
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7811..7827
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7846..7866
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7848..7866
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7894..8120
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8121..8301
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8348..8473
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8966..9199
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9384..9428
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9641..9758
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cagcagagat ccactttggc cgcgaatcga tatgtcgagt ttactcccta tcagtgatag
       61 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag
      241 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta
      301 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt
      361 ggctagcgcc accatggaag atccgcgtag aaggacgacg gcgccacgcg ctaagaagcc
      421 gtccgcgaag cgcgccccga cgcagcccag caggaccagg gcccacgcgg aaagctgcgg
      481 cccgcaaagg ggggctcgat cgcggcgggc ggagcgtgac ggggacacca cggagaagcc
      541 acgtgcccca gggccccgag tgcatccagc aagggccact gagctcacca aagatgcaca
      601 gccctcggcc atggacgcgg caggagccac cgcgcggcct gccgtccggg tgccccagca
      661 gcaggccatc ctggatccgg agctgcccgc cgtacgggag ccccagccgc ccgcggatcc
      721 cgaggcgcgg aaagtcgtaa ggggacctag ccacagaagg ggcgcgcgct ccaccgggca
      781 gcccagagcg ccgcgagggt ccaggaagga accggacaag ctaaagaagg tgctggacaa
      841 attgagattg aaacgcaaag atatctcgga ggcggccgag acggtgaata aagttgtgga
      901 acgcctgctg cgcagaatgc agaaacggga gtcggagttc aaaggtgtgg agcagctgaa
      961 cactggcagc tactatgaac atgtgaagat ttctgctcct aatgaatttg atgttatgtt
     1021 taaactggaa gtccccagga ttgagctaca agaatattat gaaacaggtg ctttctatct
     1081 tgtgaaattc aaaagaattc cacgaggaaa tccgctgagt catttcttag aaggggaagt
     1141 attatcagct accaagatgc tgtcaaagtt taggaaaatc attaaagaag aagttaaaga
     1201 aatcaaagat atagatgtca gtgtggagaa ggaaaaacca ggaagccctg ctgtaacact
     1261 tcttatcagg aaccctgaag aaatctctgt ggatataatt ctggctttgg agtcaaaagg
     1321 cagctggcct attagtacca aagaaggact acctattcaa ggctggctgg gcacaaaagt
     1381 gaggaccaat ctaagacgag agccgtttta tctcgtaccc aagaatgcaa aggatggaaa
     1441 tagttttcaa ggagagacct ggcgcctctc tttctctcac actgaaaagt acattttgaa
     1501 taatcacggg atagagaaaa catgctgtga atcttccgga gcaaaatgct gcagaaaaga
     1561 atgtttaaaa ttaatgaaat accttttgga acagttgaaa aaagagtttc aagagctgga
     1621 tgcattctgt tcctaccatg tgaaaactgc catctttcac atgtggaccc aggacccgca
     1681 ggacagtcag tgggacccca ggaacctcag ctcctgcttc gataagttgt tagcattctt
     1741 tcttgagtgc ctcaggacag agaaactgga tcattatttt attccaaagt tcaatctatt
     1801 ctctcaagaa ctaattgacc gaaaaagtaa agaatttcta tcgaagaaaa ttgaatatga
     1861 aagaaataat gggtttccaa tttttgacaa gcttgcggcc gcaatcgatg tcgaggatta
     1921 caaggatgac gacgataagg actataagga cgatgatgac aaggactaca aagatgatga
     1981 cgataaatag gtcgacaccg gttccggagc cacgaacttc tctctgttaa agcaagcagg
     2041 agacgtggaa gaaaaccccg gtcctaggct gcagacgcgt tgtacaggat ccggggttgg
     2101 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     2161 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     2221 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     2281 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     2341 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     2401 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     2461 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     2521 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     2581 gaatcaccga cctctctccc cagcaattca cccgcgcgcg atgaaaaagc ctgaactcac
     2641 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     2701 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     2761 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     2821 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta gcgagagcct
     2881 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     2941 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     3001 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     3061 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     3121 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     3181 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     3241 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     3301 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     3361 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     3421 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     3481 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     3541 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     3601 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaattcgaag gaagcggaga
     3661 aggtagaggt tctctcctca cttgtggtga tgttgaagaa aaccctggtc caatgtctag
     3721 actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga
     3781 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     3841 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     3901 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     3961 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     4021 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     4081 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     4141 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     4201 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga
     4261 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     4321 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     4381 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     4441 tgacatgctc cccgggtaac taagtaagga tcgatccaag atatcgtatt cttaactatg
     4501 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4561 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4621 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4681 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4741 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4801 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     4861 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4921 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4981 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgttt
     5041 cgcctcggcg tccggactag aggtaccttt aagaccaatg acttacaagg cagctgtaga
     5101 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5161 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5221 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5281 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5341 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5401 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5461 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5521 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5581 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5641 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5701 ttccagaagt agtgaggagg cttttttgga ggcctttcaa atatgtatcc gctcatgaga
     5761 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5821 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5881 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5941 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6001 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6061 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6121 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6181 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6241 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6301 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6361 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6421 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6481 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6541 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6601 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6661 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6721 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6781 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6841 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6901 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6961 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7021 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7081 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7141 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7201 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7261 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7321 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7381 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7441 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7501 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7561 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7621 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7681 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7741 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7801 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     7861 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     7921 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     7981 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     8041 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     8101 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     8161 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8221 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8281 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8341 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8401 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     8461 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     8521 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8581 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8641 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8701 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8761 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     8821 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8881 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8941 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9001 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9061 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9121 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9181 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9241 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9301 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9361 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     9421 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     9481 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     9541 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     9601 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     9661 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9721 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac aggga
//
