LOCUS       sequence-485874-        5036 bp    DNA     circular SYN 12-SEP-2025
DEFINITION  AiE1129m_3xC1 is an enhancer sequence designed to drive AAV-mediated
            transgene expression in specific populations of brain cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5036)
  TITLE     Basal Ganglia/CERP Enhancer AAVs
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 12, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP15708 -
            pAAV-AiE1129m_3xC1-minBG-SYFP2-WPRE...
FEATURES             Location/Qualifiers
     source          1..5036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1048..1057
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1054..1056,1057..1059,1060..1770)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1099..1120)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1360..1379)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1707..1728
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1863..1883)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2070..2181
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2299..2439
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2299..2428
                     /label=AAV2 ITR
     rep_origin      2514..2969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2601..2620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2811..2832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2986..3005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3105..3127
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3165..3183)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3251..3355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3356..3424,3425..4216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3574..3593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4387..4975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4876..4895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtagat ggatgggtgt atagattgat gggtatatgg
      181 atagaccagt gtatagatgg gtgggtgggt gaataggtgg tcttgtatta catcctcaag
      241 tctggtctaa tccatttctc ctgtaggact ggacatgtgg gtgccatatt tggcatttgt
      301 tctctaaaga tagcctggac tgttgtttta attttgatat atatgaagta gaattctctt
      361 cttgcagtaa ctgggtgcta aggctgagct ctcagcgggg agggccattg tactgcgtag
      421 atggatgggt gtatagattg atgggtatat ggatagacca gtgtatagat gggtgggtgg
      481 gtgaataggt ggtcttgtat tacatcctca agtctggtct aatccatttc tcctgtagga
      541 ctggacatgt gggtgccata tttggcattt gttctctaaa gatagcctgg actgttgttt
      601 taattttgat atatatgaag tagaattctc ttcttgcagt aactgggtgc taaggctgag
      661 ctctcagcgg ggagggccat tgtactgcgt agatggatgg gtgtatagat tgatgggtat
      721 atggatagac cagtgtatag atgggtgggt gggtgaatag gtggtcttgt attacatcct
      781 caagtctggt ctaatccatt tctcctgtag gactggacat gtgggtgcca tatttggcat
      841 ttgttctcta aagatagcct ggactgttgt tttaattttg atatatatga agtagaattc
      901 tcttcttgca gtaactgggt gctaaggctg agctctcagc ggggagggcc attgtactgc
      961 gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt ctgggatcca
     1021 gatctttcga agctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
     1081 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1141 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     1201 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcgtg
     1261 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1321 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1381 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1441 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1501 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     1561 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     1621 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
     1681 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1741 atcactctcg gcatggacga gctgtacaag taagtcgacg gcgcgccgcg gccgcgaatt
     1801 cgatatcata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     1861 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     1921 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg
     1981 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2041 gacaattccg tggctcgaga gatcttcgac tgtgccttct agttgccagc catctgttgt
     2101 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     2161 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     2221 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg agatctcacg
     2281 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     2341 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     2401 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     2461 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     2521 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2581 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2641 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2701 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     2761 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2821 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
     2881 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2941 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     3001 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3061 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3121 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     3181 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     3241 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3301 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3361 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3421 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3481 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3541 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3601 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3661 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3721 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3781 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3841 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3901 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3961 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4021 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4081 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4141 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4201 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4261 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4321 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4381 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4441 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4501 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4561 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4621 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4681 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4741 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4801 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4861 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4921 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4981 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
