LOCUS       sequence-486035-        7788 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  pBBR1MCS replicative vector for Cas1-Cas2-RT and Spacer for 
            transcriptional recording with a temperature sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Nevot G, Pol Cros M, Toloza L, Campama-Sanz N, Artigues-Lleixa M, 
            Aguilera L, Guell M
  TITLE     Engineered Marine Biofilms for Ocean Environment Monitoring.
  JOURNAL   ACS Synth Biol. 2025 Jun 22. doi: 10.1021/acssynbio.5c00192.
  PUBMED    40545707
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jun 22. doi: 10.1021/acssynbio.5c00192."
COMMENT     Sequence Label: pDS_TempRecording
FEATURES             Location/Qualifiers
     source          1..7788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3425..3443)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3439..3486
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4439..4458
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4525..4627)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      6083..6854
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             6855..7517
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALIETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 aaaaaggaaa gatgtagtgg caaacctcac tcacgatgcc aaccccccgc aaacccgggc
       61 cattgaccct ggtgatgcgc cgctttccgc accggaaacc tcacccgttc caaccgtcga
      121 aaaaatttcg tgggcgggtc ttcgaccatc ttgaatccgc tccggcccct tccgatttcc
      181 gctgcgggag ggcagacctc ccggttcttt caaatggagg aagacgaaag cgtcgttcca
      241 gtcgttcggc attccgggcc agctggaagt catcaagaag gcgctggatc acgtgcgcgt
      301 cggcgtggtc atcaccgatc ccgcgctgga agataacccg atcgtctacg tgaaccaggg
      361 cttcgtgcag atgaccggct acgagaccga ggaaatcctg ggcaagaacg cgcgcttcct
      421 ccaggggaag cacaccgatc cggcggaagt ggacaacatc cgcaccgcgc tgcaaaataa
      481 agaaccggtc accgtgcaga tccagaacta caagaaggac ggcacgatgt tctggaacga
      541 actgaacatc gatccgatgg aaatcgagga taagacgtat ttcgtcggca tccagaacga
      601 catcaccaag cagaaggaat atgaaaagct gctctaaaat aaccccttgg ggcctctaaa
      661 cgggtcttga ggggtttttt gcggcatttg agaagcacac ggtcacactg cttccggtag
      721 tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac
      781 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
      841 tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc
      901 gccctgccac tcatcgcagt acggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      961 ttaacgcgaa ttttaggagg aagagctcac tcattaggca ccccaggccc ggaattgcca
     1021 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     1081 ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg
     1141 tttcgcatga ttgaacaaga tggattgcac gcaggttcta tgtttaccat cgacgagatg
     1201 ctgagcaaga acaaccaacg tctggcgttc gaacactttg cgaccaaaaa cgacggttgc
     1261 ggcccggacg gtatgcacgt tagcgaactg gagaagtact ggcgtatgaa tcacgaccag
     1321 atcattagcg atctgaagaa ccaggaatac cagccgggta ttatcctgat tcgtgagcac
     1381 atgaataaaa ccggcaaacg ccgtaatatc gcgagcctga acgttattga tcgtttcatt
     1441 acccgtctgc tgagccagaa actgaaccgt tatctggcgc cgattttctg cgagaacagc
     1501 tatgcgtacc aggacagcaa aggcgttatg ccggcggttc tgaaagcgaa ggagtacgtt
     1561 gagctgggta tgcgtcacgt gattgaaatc gatctgaaaa actactttga caccattccg
     1621 ctggaaaacc tgatcccgga gatcgaacgc tacatcaccg atgaggcggt tctgcatctg
     1681 atcaagcaat acctgttttg cgatattagc ttcgagggca aaatcagccg taaaacccaa
     1741 ggtatcgttc aaggtaacgc gatcagcccg atcctgagca acctgtacct gaacgacttc
     1801 gacaaggaac tggacgagag caagctgtgc tggattcgtt acgcggacaa tatctacatc
     1861 tatatggata gctatgagaa ggcgctgctg gtgtatagcg agctgaccga gcgtctggag
     1921 cgtcgcaagc tgaccgttaa caaggagaaa agcggcgttt ttgatgtgag cacccgtagc
     1981 attctgggct acgacatcct gattcgtaac aagaaagtgg acgttcgtaa gcatatttac
     2041 aaaagcgtta atcagtacag caactggcac gatagccgcc tggagtttat caacggtcgt
     2101 taccacatca ccagcgatgg catcctgaat cgccaagact tcggcctgct gttcgagaat
     2161 gagcagaaaa aacactacat cccggttgag gttagcgatc agctgaacat ctacggtaat
     2221 gttaccctgg cgagcaatgt gctgcaaagt ttcagcaacc gcgaaatcaa agtgagcttc
     2281 ttcgacaagt atggtcgtct gattggtagc ttcctgccgg aaaaaaccaa gaaaagcgcg
     2341 gagatcattc tggtgcagag caagaactat ctgaacgaag atgtgcgtat ggataccgcg
     2401 cgccgtatgg aaattgcggg tctgcacaac atccgtgcga atctgcgcta ctacgataag
     2461 aaacacaagg gcgactttaa agagaaggtt gacgcgatta gcggctacat tgacgcgctg
     2521 aaccgtgcgc cgagcgttaa tgatatgatg ctgctggaag cgaaagcgcg ccaactgtat
     2581 tatacctgtt ttaaccaaat cctggaaacc agcgacttcc agttcgaaaa gcgtaccaag
     2641 cgtccgccga aagatgcgat caatgcgtgc attagcttcg gcaataccct gctgtataat
     2701 ctatttgtga atattatctg gaagaagggt ctggacccgc gctttggcgt tgttcacgcg
     2761 agcaacaaac gcaatcaaag cctgaacctg gacttcgcgg acatctttaa accgatcgtg
     2821 attgaccgca ttatcttcac catgatcaat aagaaaatgc tgaccctgct gaccgatttt
     2881 gaaaccagca accagggtgt gtatctgagc cgtgagggca agaacatctt cctgcaaatg
     2941 tacgaggaga agctgaaaag ccgcattacc atcaagggta aagagatgag ctattaccag
     3001 ctgctggaga gcgaagtgca gaattacaag aatttcattc tgaccggcga aacctataaa
     3061 ccgtataagt attattaacg tacgttaact ttaagaagga gaacttaaga tgtatgttat
     3121 cctggtgtac gacatccacc agaagcgtgt tggtaaagcg ctgaaaatct gccgtaagta
     3181 cctgatccac atccaaaaga gcgtgttcga aggcaacatc accgagagca aactgaaggc
     3241 gctgaaagag gaactgggtc acctgattga cacccagatg gatagcgtga ttatctacca
     3301 cctggacagc gttaagtaca ccaaaaagga gcagatcggt attgtgcaga gcaccagcaa
     3361 tgtgatctaa ctcgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     3421 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     3481 tttttgctga aaggaggaac tatatccgga taccaggtgc ggggaagctt atgttccata
     3541 gcaaaaagtc ggtcagtctc gtggctgaaa tcatgagttc cacaaaatgg ctgaaattca
     3601 aggaaaatca ggaatctcag aaaaacgatc gaccgacttt ttcgataaaa tggttgcaaa
     3661 aatgagaaaa atctgattta atagaatctg aaaacagcgg aaatgctgtt gtcgtacttt
     3721 acctaaaagg aattgaaacg tcccgcggcc gctattcttt tgatttataa gggattttgc
     3781 cgatttcggc ctattggtcg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3841 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata aaaactgttg
     3901 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     3961 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     4021 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     4081 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     4141 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     4201 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     4261 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
     4321 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     4381 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     4441 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     4501 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     4561 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     4621 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     4681 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cgaagacgaa
     4741 aggtcctcgt gatacgccta tttttatagg ttaatgatcg ccatgtaagc ccactgcaag
     4801 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     4861 tcatcccagg tggcactttt cggggaaatg tgcgcgcccg cgttcctgct ggcgctgggc
     4921 ctgtttctgg cgctggactt cccgctgttc cgtcagcagc ttttcgccca cggccttgat
     4981 gatcgcggcg gccttggcct gcatatcccg attcaacggc cccagggcgt ccagaacggg
     5041 cttcaggcgc tcccgaaggt ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat
     5101 ctcacgcgct cctgcggcgg cctgtagggc aggctcatac ccctgccgaa ccgcttttgt
     5161 cagccggtcg gccacggctt ccggcgtctc aacgcgcttt gagattccca gcttttcggc
     5221 caatccctgc ggtgcatagg cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc
     5281 cactggtggc cgctccaggg cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct
     5341 gccctcgatg ccccgttgca gccctagatc ggccacagcg gccgcaaacg tggtctggtc
     5401 gcgggtcatc tgcgctttgt tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt
     5461 cagcggcacc acgaacgcgg tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt
     5521 cacgatgcga tccgccccgt acttgtccgc cagccacttg tgcgccttct cgaagaacgc
     5581 cgcctgctgt tcttggctgg ccgacttcca ccattccggg ctggccgtca tgacgtactc
     5641 gaccgccaac acagcgtcct tgcgccgctt ctctggcagc aactcgcgca gtcggcccat
     5701 cgcttcatcg gtgctgctgg ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc
     5761 gttgggcgtc tcgcgctcgc ggtaggcgtg cttgagactg gccgccacgt tgcccatttt
     5821 cgccagcttc ttgcatcgca tgatcgcgta tgccgccatg cctgcccctc ccttttggtg
     5881 tccaaccggc tcgacggggg cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg
     5941 agtatactca ctagactttg cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc
     6001 tacgggcttg ctctccgggc ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg
     6061 gatatgtgga tgatggccgc gagcggccac cgactggctc gcttcgctcg gcccgtggac
     6121 aaccctgctg gacaagctga tggacaggct gcgcctgccc acgagcttga ccacagggat
     6181 tgcccaccgg ctacccagcc ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg
     6241 gccttgcccc atcaattttt ttaattttct ctggggaaaa gcctccggcc tgcggcctgc
     6301 gcgcttcgct tgccggttgg acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg
     6361 cgcagcggct tggccttgac gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc
     6421 gaagggcgaa gcccgcccgc ctgccccccg agcctcacgg cggcgagtgc gggggttcca
     6481 agggggcagc gccaccttgg gcaaggccga aggccgcgca gtcgatcaac aagccccgga
     6541 ggggccactt tttgccggag ggggagccgc gccgaaggcg tgggggaacc ccgcaggggt
     6601 gcccttcttt gggcaccaaa gaactagata tagggcgaaa tgcgaaagac ttaaaaatca
     6661 acaacttaaa aaaggggggt acgcaacagc tcattgcggc accccccgca atagctcatt
     6721 gcgtaggtta aagaaaatct gtaattgact gccactttta cgcaacgcat aattgttgtc
     6781 gcgctgccga aaagttgcag ctgattgcgc atggtgccgc aaccgtgcgg cacccctacc
     6841 gcatggagat aagcatggcc acgcagtcca gagaaatcgg cattcaagcc aagaacaagc
     6901 ccggtcactg ggtgcaaacg gaacgcaaag cgcatgaggc gtgggccggg cttattgcga
     6961 ggaaacccac ggcggcaatg ctgctgcatc acctcgtggc gcagatgggc caccagaacg
     7021 ccgtggtggt cagccagaag acactttcca agctcatcgg acgttctttg cggacggtcc
     7081 aatacgcagt caaggacttg gtggccgagc gctggatctc cgtcgtgaag ctcaacggcc
     7141 ccggcaccgt gtcggcctac gtggtcaatg accgcgtggc gtggggccag ccccgcgacc
     7201 agttgcgcct gtcggtgttc agtgccgccg tggtggttga tcacgacgac caggacgaat
     7261 cgctgttggg gcatggcgac ctgcgccgca tcccgaccct gtatccgggc gagcagcaac
     7321 taccgaccgg ccccggcgag gagccgccca gccagcccgg cattccgggc atggaaccag
     7381 acctgccagc cttgatcgaa acggaggaat gggaacggcg cgggcagcag cgcctgccga
     7441 tgcccgatga gccgtgtttt ctggacgatg gcgagccgtt ggagccgccg acacgggtca
     7501 cgctgccgcg ccggtagcac ttgggttgcg cagcaacccg taagtgcgct gttccagact
     7561 atcggctgta gccgcctcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     7621 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca
     7681 ccgtttttat caggctctgg gaggcagaat aaatgatcat atcgtcaatt attacctcca
     7741 cggggagagc ctgagcaaac tggcctcatt tgaagggtat ttgctgga
//
