LOCUS       sequence-486051-        8473 bp    DNA     circular SYN 04-NOV-2025
DEFINITION  Control circuit for Re-NF-FF controller.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Zhang R, Zhang R, Tian XJ
  TITLE     Integrating recombinase-based feedback and feedforward control for 
            optimal resource decoupling.
  JOURNAL   Nucleic Acids Res. 2025 Sep 5;53(17):gkaf913. doi: 
            10.1093/nar/gkaf913.
  PUBMED    40966512
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-09-5"; volume: "53(17):gkaf913. doi"; 
            pages: " 10.1093/nar/gkaf913"
COMMENT     Sequence Label: Circuit C55
FEATURES             Location/Qualifiers
     source          1..8473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             832..843
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(855..1532)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPYDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             1539..1550
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1899..1969
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1985..2012
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2081..2115
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2124..2135
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2142..2855
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2172..2200)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2795..2815
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2878..2949
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2965..2992
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3007..3041
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             3050..3061
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3068..3946
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      3968..4039
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4055..4082
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        4097..4131
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     CDS             4160..5662
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLEYCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLVGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      5681..5752
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5768..5795
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    5802..5822
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(5962..6777)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5962..6777)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6685..6704
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6769..6788
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(7372..7916)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7451..7470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8384..8402
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8406..8449)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    8452..8473
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcgaaaaaac cccgccgaag cggggttttt tgcgtactag agttattaac cggtgacttc
       61 ccagacctgg ccgtcaacgg tgaacttgcc tcccaggatc gggacgatct cagccttgac
      121 atgcttgccg tcgaccgtga gcatcccgaa gcccatctgc cagttcccgg ctccgccctt
      181 tagatagttg gccttcttca tgtccatcag gtgcccgact tccatgccgg tgacggtctt
      241 gcgcaccgag ccgccgtacc cgaacgagtg cgagacgaca gcctgccggt gcgtgtggcc
      301 gcagaccacg gacttgccga acttcttggc accgttgagc gctgtcgatc cggcgatctg
      361 ggatagcgtc atcttgccca tgtgcccgtg agtggagatc cagcccggag cgatgtcgta
      421 gaagtcaggc agcagctcca caccgaaccc gtcgaagtcg agcagcacgt cgatgtcgaa
      481 agcgtgcgta ccctccaggg ccggtgcgtt cttggccagg tactcgcggg ctcgcagatc
      541 gtggttgccc tcgtgcatcc cgatccagcc gtcgtagacc ttgcgcagcg gctccatcag
      601 gttcttcttg gcgtagtccg cgtcgcggta caccgagccc tcgaactcgc ccttggttcc
      661 ccggttccag cgcgagggct gcggcaggtc taggacgtca ccgatatgta ccacgccgta
      721 cggctggaca tccccgatga agcggatgac cgcttgcatc tctttgcgcg cctcgaaagg
      781 caactgagta tcgggtagaa agacgatacg ctgagtgccg gccatctagt atttctcctc
      841 ttttactaga gttattaagc accggtggag tgacgacctt cagcacgttc gtactgttca
      901 acgatggtgt agtcttcgtt gtgggaggtg atgtccagtt tgatgtcggt tttgtaagca
      961 cccggcagct gaaccggttt tttagccatg taggtggttt taacttcagc gtcgtagtga
     1021 ccaccgtctt tcagtttcag acgcattttg atttcacctt tcagagcacc gtcttccggg
     1081 tacatacgtt cggtggaagc ttcccaaccc atggtttttt tctgcataac cggaccgtcg
     1141 tacgggaagt tggtaccacg cagtttaact ttgtagatga actcaccgtc ttgcagggag
     1201 gagtcctggg taacggtaac aacaccaccg tcttcgaagt tcataacacg ttcccatttg
     1261 aaaccttccg ggaaggacag tttcaggtag tccgggatgt cagccgggtg tttaacgtaa
     1321 gctttggaac cgtactggaa ctgcggggac aggatgtccc aagcgaacgg cagcggacca
     1381 cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca
     1441 ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg
     1501 aactctttga taacgtcttc ggaggaagcc atctagtatt tctcctcttt tactagagcg
     1561 gccggcttgt cgacgacggc gcactcagtg gtgtacggta caaaccccga caatagcgag
     1621 gaggtaaatt gtaagcttta gggaaacgac atttactgac gcgactttct caaagctagg
     1681 gtgttggcct gaactgtacg cttggagttg taggacagac ataaaagtat cagatacgca
     1741 cactaaccat ctgctagccc aaaaaaacgg tatggagaaa cagtagagag ttgcgataaa
     1801 aagcgtcagg taggatccgc taatcttatg gactatcttg ctatggcata gcaaagtgtg
     1861 acgccgtgca aataatcaat gtctctagta ccaggcatca aataaaacga aaggctcagt
     1921 cgaaagactg ggctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1981 cactggctca ccttcgggtg ggcctttctg cgtttatagc ccggatgatc ctgacgacgg
     2041 agaccgcggt ggttgaccag acaaaccacg actactagag tttacggcta gctcagtcct
     2101 aggtatagtg ctagctacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac
     2161 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     2221 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     2281 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
     2341 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     2401 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     2461 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     2521 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     2581 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     2641 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     2701 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     2761 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     2821 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
     2881 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2941 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     3001 ctagagttta cggctagctc agtcctaggt acaatgctag ctactagaga aagaggagaa
     3061 atactagatg gctgaagcgc aaaatgatcc cctgctgccg ggatactcgt ttaacgccca
     3121 tctggtggcg ggtttaacgc cgattgaggc caatggttat ctcgattttt ttatcgaccg
     3181 accgctggga atgaaaggtt atattctcaa tctcaccatt cgcggtcagg gggtggtgaa
     3241 aaatcaggga cgagaatttg tctgccgacc gggtgatatt ttgctgttcc cgccaggaga
     3301 gattcatcac tacggtcgtc atccggaggc tcgcgaatgg tatcaccagt gggtttactt
     3361 tcgtccgcgc gcctactggc atgaatggct taactggccg tcaatatttg ccaatacggg
     3421 tttctttcgc ccggatgaag cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat
     3481 taacgccggg caaggggaag ggcgctattc ggagctgctg gcgataaatc tgcttgagca
     3541 attgttactg cggcgcatgg aagcgattaa cgagtcgctc catccaccga tggataatcg
     3601 ggtacgcgag gcttgtcagt acatcagcga tcacctggca gacagcaatt ttgatatcgc
     3661 cagcgtcgca cagcatgttt gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca
     3721 gttagggatt agcgtcttaa gctggcgcga ggaccaacgc atcagccagg cgaagctgct
     3781 tttgagcact acccggatgc ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca
     3841 actctatttc tcgcgagtat ttaaaaaatg caccggggcc agcccgagcg agttccgtgc
     3901 cggttgtgaa gaaaaagtga atgatgtagc cgtcaagttg tcataataaa ctactagagc
     3961 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     4021 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     4081 cgtttatata ctagagttta cggctagctc agtcctaggt actatgctag ctactagagt
     4141 cacacaggaa acctactaga tgagagccct ggtagtcatc cgcctgtccc gcgtcaccga
     4201 tgctacgact tcaccggagc gtcagctgga gtattgccag cagctctgcg cccagcgcgg
     4261 ctgggacgtc gtcggggtag cggaggatct ggacgtctcc ggggcggtcg atccgttcga
     4321 ccggaagcgc agaccgaacc tggcccggtg gctagcgttc gaggagcaac cgtttgacgt
     4381 gatcgtggcg taccgggtag atcggttgac ccgatcgatc cggcatcttc agcagctggt
     4441 ccactgggcc gaggaccaca agaagctggt cgtctccgcg accgaagcgc acttcgatac
     4501 gacgacgccg tttgcggcgg tcgtcatcgc gcttatggga acggtggcgc agatggaatt
     4561 agaagcgatc aaagagcgga accgttcggc tgcgcatttc aatatccgtg ccgggaaata
     4621 ccgaggatcc ctgccgccgt ggggatacct gcctacgcgc gtggacgggg agtggcggct
     4681 ggtgccggac cctgtgcagc gagagcgcat cctcgaggtg tatcaccgcg tcgtcgacaa
     4741 ccacgagccg ctgcatctgg tggcccacga cctgaaccgg cgtggtgtcc tgtcgccgaa
     4801 ggactacttc gcgcagctgc aaggccgcga gccgcagggc cgggagtggt cggctaccgc
     4861 gctgaagcga tcgatgatct ccgaggcgat gctcgggtac gcgactctga acggtaagac
     4921 cgtccgagac gacgacggag ccccgctggt gcgggctgag ccgatcctga cccgtgagca
     4981 gctggaggcg ctgcgcgccg agctcgtgaa gacctcccgg gcgaagcccg cggtgtctac
     5041 cccgtcgctg ctgctgcggg tgttgttctg cgcggtgtgc ggggagcccg cgtacaagtt
     5101 cgccggggga ggacgtaagc acccgcgcta ccgctgccgc tcgatggggt tcccgaagca
     5161 ctgcgggaac ggcacggtgg cgatggccga gtgggacgcg ttctgcgagg agcaggtact
     5221 ggatctgctc ggggacgcgg agcgtctgga gaaagtctgg gtagcgggct cggactccgc
     5281 ggtcgaactc gcggaggtga acgcggagct ggtggacctg acgtcgctgg tcggctcccc
     5341 ggcctaccgg gcgggctctc cgcagcgaga agcactggat gcccgtattg cggcgctggc
     5401 cgcgcggcaa gaggagctgg agggcctgga ggctcgcccg tctggctggg agtggcgcga
     5461 gaccgggcag cggttcgggg actggtggcg ggagcaggac accgcggcaa agaacacctg
     5521 gcttcggtcg atgaacgttc ggctgacgtt cgacgtccgc ggcgggctga ctcgcacgat
     5581 cgacttcggg gatcttcagg agtacgagca gcatctcagg ctcggcagcg tggtcgaacg
     5641 gctacacacc gggatgtcgt aataaactag agccaggcat caaataaaac gaaaggctca
     5701 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     5761 tcacactggc tcaccttcgg gttggccttt ctgcgtttat atactagtag cggccgctgc
     5821 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5881 ctcactcaaa ggcggtaatc tcgagtcccg tcaagtcagc gtaatgctct gccagtgtta
     5941 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
     6001 attcatatca ggattatcga taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     6061 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     6121 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     6181 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt
     6241 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     6301 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
     6361 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     6421 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
     6481 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     6541 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     6601 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     6661 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     6721 catgttggaa tttaatcgcg gcctggagca agacgtttcc cgttgaatat ggctcataac
     6781 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     6841 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg
     6901 aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt
     6961 ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg
     7021 tggctccctc actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac
     7081 accttcttca cgaggcagac ctcagcgcta gcggagtgta tactggctta ctatgttggc
     7141 actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc
     7201 gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct
     7261 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
     7321 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
     7381 gctccgcccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     7441 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
     7501 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     7561 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     7621 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     7681 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     7741 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     7801 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcaaaaaa ccgccctgca
     7861 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     7921 atcatcttat taaggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7981 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8041 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8101 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8161 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8221 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8281 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattcca tggtgccacc
     8341 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     8401 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg
     8461 gccgcttcta gag
//
