LOCUS       sequence-486061-        9773 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus 
            and 4xSV40 NLS at C-temrinus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Ma E, Chen K, Shi H, Wasko KM, Esain-Garcia I, Trinidad MI, Zhou K, 
            Ye J, Doudna JA
  TITLE     Directed evolution expands CRISPR-Cas12a genome-editing capacity.
  JOURNAL   Nucleic Acids Res. 2025 Jul 8;53(13):gkaf649. doi: 
            10.1093/nar/gkaf649.
  PUBMED    40671523
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-07-8"; volume: "53(13):gkaf649. doi"; 
            pages: " 10.1093/nar/gkaf649"
COMMENT     Sequence Label: pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS
FEATURES             Location/Qualifiers
     source          1..9773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2665..2684
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2768..2787
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2768..2786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2875..2897
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2917..2946
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2956..4056
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     4030..4053
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             4111..4131
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             7831..7851
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7861..7881
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7891..7911
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7921..7941
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8070..8088)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8084..8131
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8497..8502,8503..8519,8520..8525))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8552..8570)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8638..8742
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8743..8811,8812..9603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8961..8980)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     1681 acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     1741 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     1801 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     1861 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     1921 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     1981 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     2041 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2101 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2161 gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     2221 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     2281 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     2341 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     2401 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     2461 catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     2521 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     2581 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     2641 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     2701 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
     2761 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag tgccggctcc
     2821 ggagagctct ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt
     2881 taactttaag aaggagatat acatatgaaa tcttctcacc atcaccatca ccatcaccat
     2941 caccatggtt cttctatgaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
     3001 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
     3061 gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc
     3121 gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc
     3181 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg
     3241 gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg
     3301 ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
     3361 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
     3421 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
     3481 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
     3541 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
     3601 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
     3661 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
     3721 tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc gaacaaagag
     3781 ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
     3841 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
     3901 ccacgtattg ccgccactat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
     3961 cagatgtccg ctttctggta tgccgtgcgt actgcggtga tcaacgccgc cagcggtcgt
     4021 cagactgtcg atgaagccct gaaagacgcg cagactaatt cgagctcgaa caacaacaac
     4081 aataacaata acaacaacct cgggatcgag gaaaacctgt acttccaatc caatgcaatg
     4141 agcaaactgg agaagttcac gaactgttat tctctgtcga aaaccttacg ttttaaggcc
     4201 attccggtag gtaaaacgca agaaaatatc gacaataaac gtctcctggt tgaagatgaa
     4261 aagcgtgccg aagattataa aggtgttaaa aagctgcttg atcgctatta cctgtctttt
     4321 atcaacgatg ttctgcattc catcaaattg aaaaacttga ataattacat ctcgttattt
     4381 cgcaaaaaaa cccgtaccga aaaagaaaac aaggagctgg aaaaccttga aattaatctg
     4441 cgtaaagaaa tcgcgaaagc atttaaaggt aatgaaggat ataaatctct gtttaaaaaa
     4501 gacattattg aaaccatcct gcctgagttc ttggatgata aagatgaaat tgccctggtt
     4561 aatagcttta atggctttac gactgcattt accggctttt ttgataaccg tgaaaacatg
     4621 ttcagcgaag aagcgaaatc gacttccatt gccttccgtt gcattaatga aaacctgacc
     4681 cgctacattt ctaatatgga catctttgaa aaagttgatg ccattttcga caaacatgaa
     4741 gttcaagaaa tcaaagaaaa gattttaaac tccgattatg atgttgaaga tttctttgag
     4801 ggagaatttt tcaattttgt tctcacccaa gagggtattg atgtctataa cgccattatc
     4861 ggaggatttg ttacggaatc aggcgaaaag attaaaggac tgaatgaata cattaattta
     4921 tacaatcaga aaaccaaaca gaaattgcca aaattcaaac cactgtataa acaggtatta
     4981 agcgaccgtg aatccttgtc cttttatggc gaaggctata caagcgatga agaggtgctc
     5041 gaagtattcc gtaatacttt gaacaaaaac tcggaaatct tctccagcat taaaaaactg
     5101 gaaaaattgt ttaaaaattt cgatgaatat agtagcgcag gcatcttcgt caaaaatggc
     5161 cctgcgattt caaccatttc taaagacatt ttcggtgaat ggaacgtgat ccgcgataaa
     5221 tggaacgcgg agtatgacga cattcatctg aaaaaaaaag ccgttgttac cgaaaagtat
     5281 gaagatgacc gccgcaagag ctttaaaaaa attggtagtt tctcactgga acagttacaa
     5341 gaatatgcag acgccgatct gtcggttgtg gaaaagttaa aagagattat tatccagaaa
     5401 gtcgatgaaa tttataaagt gtacggctcc agcgaaaagc tctttgatgc cgactttgtt
     5461 ttagaaaaat cgctgaaaaa aaacgatgcc gtggtggcca tcatgaaaga tctgttggat
     5521 tcggtcaaat cgttcgaaaa ctatatcaag gcgttctttg gcgaagggaa agagaccaat
     5581 cgtgacgaat cgttctatgg cgattttgtg ttggcgtatg atattcttct gaaagtagat
     5641 cacatctatg atgctatccg caactacgtg acccagaaac cgtattcgaa ggataaattt
     5701 aagctgtatt ttcaaaaccc tcagtttatg ggtggctggg gtaaagataa agaaaccgac
     5761 taccgcgcga cgatcttgcg ctatggcttt aagtactatt tagccatcat ggataaaaag
     5821 tatgcaaaat gcctccagaa aatcgataag gatgatgtca acgggaacta cgaaaaaatc
     5881 aactacaaac tcttgcctgg cccgaataaa atgctgccga aagtcttttt ctccaaaaaa
     5941 tggatggcct attataaccc ttccgaagat attcagaaga tttacaagaa cggtaccttt
     6001 aaaaaaggtg acatgtttaa tctgaatgac tgtcacaagc ttattgattt cttcaaagat
     6061 agtatttctc gctaccctaa atggtcaaac gcgtacgatt ttaatttctc agaaacagag
     6121 aaatacaaaa atattgcggg gttttatcgc gaagtcgaag aacaagggta taaggtgtcg
     6181 ttcgaatcgg cttccaaaaa ggaagtggat aaacttgttg aagaaggcaa actctatatg
     6241 ttccagatct acaacaaaga tttctccgac aagagccatg gcacgccgaa cttacatacc
     6301 atgtacttta aattactgtt cgatgaaaat aatcatggtc agattcgctt gagcggtggc
     6361 gccgaactgt ttatgcgccg cgcatcattg aaaaaggaag aacttgttgt gcacccagca
     6421 aacagtccga ttgcaaacaa aaatccggat aacccgaaga aaactaccac cttaagctat
     6481 gatgtatata aagataaacg ttttagcgaa gatcagtatg aactccacat tccgatcgcg
     6541 attaacaaat gtcctaagaa catctttaaa atcaataccg aggttcgtgt actgctcaaa
     6601 cacgacgaca acccctatgt tattggcatt gaccgcggcg aacgcaacct gctgtatatt
     6661 gtcgttgtgg atggcaaagg taacattgtt gaacagtata gccttaatga aatcatcaat
     6721 aatttcaacg gcattcgtat taaaacggac tatcatagcc tgctggacaa aaaagagaaa
     6781 gaacgcttcg aagcccgtca gaattggacc tctatcgaaa atatcaagga actgaaagct
     6841 gggtacattt ctcaagtggt ccacaagatt tgcgaactgg tggaaaagta tgacgcggtg
     6901 atcgcactgg aggatctgaa ttcgggcttt aaaaatagcc gtgtgaaggt ggagaaacag
     6961 gtttaccaaa agttcgaaaa aatgctgatt gacaaactta attacatggt ggacaaaaaa
     7021 tctaatccat gtgcgaccgg cggggctctc aaaggctatc agattacgaa taaattcgaa
     7081 agcttcaaat ccatgtctac ccagaacgga tttatctttt acatccccgc ctggctgact
     7141 tccaaaattg atccgagtac cggttttgtt aatctgctga aaaccaaata tacgtcgatc
     7201 gccgattcaa aaaaattcat tagcagcttc gaccgtatta tgtacgtccc ggaagaagac
     7261 ctgttcgaat tcgcccttga ctataaaaat ttctcgcgta cggatgccga ttatatcaaa
     7321 aaatggaaac tgtacagcta cggcaaccgc atccgcattt ttcgcaaccc aaagaagaac
     7381 aacgtatttg actgggagga agtttgcttg acgagcgctt acaaagagct ctttaacaaa
     7441 tatgggatca attatcagca aggggacatc cgtgcgttgc tgtgtgagca gtcagataaa
     7501 gcgttctaca gctctttcat ggctttaatg tcgctgatgt tgcagatgcg caattcgatc
     7561 actggccgca ccgatgtgga tttcctgatt agcccggtca aaaatagcga tggcattttt
     7621 tacgattcac gtaactatga agcccaggaa aacgctattc tgccgaagaa cgctgatgcg
     7681 aacggcgcgt acaatattgc ccgtaaggta ctgtgggcaa ttggtcagtt caagaaagcg
     7741 gaagatgaaa agttggataa ggtcaaaatt gcgatctcga ataaagaatg gctggaatat
     7801 gcgcagacca gcgtgaaaca tggcggatca ccgaagaaga aacgcaaagt cggaggtagc
     7861 cctaagaaga agcgtaaggt tggggggtcg ccaaagaaga aacgtaaagt tgggggctcg
     7921 ccgaaaaaga aacgcaaggt gtgataacat tggaagtgga taacggatcc gcgatcgcgg
     7981 cgcgccacct ggtggccggc cggtaccacg cgtgcgcgct gatccggctg ctaacaaagc
     8041 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     8101 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc
     8161 acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga
     8221 gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa
     8281 attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat
     8341 ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
     8401 ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct
     8461 gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct
     8521 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     8581 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     8641 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8701 taaccctgat aaatgcttca ataacattga aaaaggaaga gtatgagtat tcaacatttc
     8761 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8821 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8881 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8941 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     9001 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9061 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9121 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9181 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9241 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     9301 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9361 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9421 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9481 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9541 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9601 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9661 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9721 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
