LOCUS       sequence-486094-        2457 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  pUC19-SARS-CoV-2-N-gene-truncated plasmid is a plasmid that contains
            the SARS-CoV-2 N-gene and can be used as a positive control in 
            SARS-CoV-2 research or diagnostics assays targeting the N gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ajibode ET, Bender AR, Yehl K
  TITLE     Profiling crRNA Architectures for Enhanced Cas12 Biosensing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUC19-SARS-CoV-2-N-gene-truncated
FEATURES             Location/Qualifiers
     source          1..2457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     405..427
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(465..483)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        551..655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(656..724,725..1516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(874..893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1687..2275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2176..2195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2429..2446
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac cattggccgc aaattgcaca
      241 atttgccccc agcgcttcag cgttcttcgg aatgtcgcgc attggcatgg aagtcacacc
      301 ttcgggaacg ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      361 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      421 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
      481 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      601 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      661 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      721 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      781 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      841 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      901 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      961 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1021 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1081 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1141 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1201 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1261 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1321 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1381 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1441 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1501 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1561 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1621 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1681 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1741 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1801 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1861 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1921 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1981 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2041 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2101 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2161 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2221 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2281 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2341 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2401 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaga
//
