LOCUS       sequence-486103-        4888 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Targeting pOX38 plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Tsoi R, Son HI, Hamrick GS, Tang K, Bethke JH, Lu J, Maddamsetti R, 
            You L
  TITLE     A predatory gene drive for targeted control of self-transmissible 
            plasmids.
  JOURNAL   Sci Adv. 2025 Apr 4;11(14):eads4735. doi: 10.1126/sciadv.ads4735. 
            Epub 2025 Apr 2.
  PUBMED    40173243
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-04-4"; volume: "11(14):eads4735. doi"; pages: " 
            10.1126/sciadv.ads4735. Epub 2025 Apr 2"
COMMENT     Sequence Label: DoS2-pOX38
FEATURES             Location/Qualifiers
     source          1..4888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(747..841)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(862..1521)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1436..1455
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1522..1624)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     oriT            1639..1928
                     /note="origin of transfer for the bacterial F plasmid 
                     (Frost et al., 1994)"
     terminator      3112..3159
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3181..3807)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     3712..3731
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        3823..3878
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    3859..3877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      4708..4794
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
      781 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      841 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
      901 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
      961 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1021 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1081 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1141 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1201 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     1261 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     1321 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1381 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1441 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1501 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1561 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     1621 atcaacgtct caaccggtag gctcaacagg ttggtggttc tcaccaccaa aagcaccaca
     1681 ccccacgcaa aaacaagttt ttgctgattt ttctttataa atagagtgtt atgaaaaatt
     1741 agtttctctt actctcttta tgatatttaa aaaagcggtg tcggcgcggc tacaacaacg
     1801 cgccgacacc gttttgtagg ggtggtactg actattttta taaaaaacat tattttatat
     1861 taggggtgct gctagcggcg cggtgtgttt ttttatagga taccgctagg ggcgctgcta
     1921 gcggtgcgtt gtgacaaatt gccctttcag tgtgacaaat caccctcaaa tgacagtcct
     1981 gtctgtgaca aattgccctt aaccctgtga caaattgccc tcagtgaggg ttatctgtca
     2041 cagatttgag ggtggttcgt cacatttgtt ctgacctact gagggtaatt tgtcacagtt
     2101 ttgctgtttc cttcagcctg catggatttt ctcatacttt ttgaactgta atttttaagg
     2161 aagccaaatt tgagggcagt ttgtcacagt tgatttcctt ctctttccct tcgtcatgtg
     2221 acctgatatc gggggttagt tcgtcatcat tgatgagggt tgattatcac agatataagc
     2281 tatgtcaggc agatggaaag ctggatgatc accacccggc cccgtcgtcg tgaaccatta
     2341 tgggccgtga ccgacgaaac catgcgcaac tggctgaagc aggctgtcag acgggccgaa
     2401 gctgacggag tacacttttc gattccggtc acaccacaca ccttccggca cagctatatc
     2461 atgcacatgc tctatcaccg ccagccccgg aaagtcatcc aggcactggc tggtcacagg
     2521 gatccacgtt cgatggaggt ttataccaga gtgtttgcac tggatatggc tgccacgctg
     2581 gcagtgcctt tcacaggtga cggtcgggat gctgcggaga tcctgcgtac actgcctccc
     2641 ctgaagtgac ggcatacgcg cttccttata taagctgtgg tcagcagaac agacataaca
     2701 taagctggag caggtagata agctgtagtg agtaaaccat gttttatgaa gggagcaatg
     2761 cctcagcatc aggttacggg gtgactcacg taagggacag gcagatggca gctcagccac
     2821 aggcagcact gcaggaaact gaatataaac agcagtgagc agaccactca ctgcacctga
     2881 taatataagc tgtagtcagt aaaggagcac tcttcactga ctacagttta tgtattactg
     2941 aatacagctt atcatgaacc tgttacagtt tatccataac atgccacagc ttatgttccc
     3001 cgcatagctg aattgttggc tatacggttt aagtggatcc cggtaatctt ctcctcgtcg
     3061 ccaaactaga tgaagattat cggggtttta cgcgttaggc gtatcacgag gctagcataa
     3121 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc gtcttcacct cgaggcatgc
     3181 ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc
     3241 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
     3301 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
     3361 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
     3421 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
     3481 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
     3541 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
     3601 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
     3661 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
     3721 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
     3781 tttactttta tctaatctag acatcattaa ttcctaattt ttgttgacac tctatcgttg
     3841 atagagttat tttaccactc cctatcagtg atagagaaaa gaattcaaaa gatctaaaga
     3901 ggagggtacc tatatgcata tgaaaaacat caaaaaaaac caggtaatga acctgggtcc
     3961 gaactctaaa ctgctgaaag aatacaaatc ccagctgatc gaactgaaca tcgaacagtt
     4021 cgaagcaggt atcggtctga tcctgggtga tgcttacatc cgttctcgtg atgaaggtaa
     4081 aacctactgt atgcagttcg agtggaaaaa caaagcatac atggaccacg tatgtctgct
     4141 gtacgatcag tgggtactgt ccccgccgca caaaaaagaa cgtgttaacc acctgggtaa
     4201 cctggtaatc acctggggcg cccagacttt caaacaccaa gctttcaaca aactggctaa
     4261 cctgttcatc gttaacaaca aaaaaaccat cccgaacaac ctggttgaaa actacctgac
     4321 cccgatgtct ctggcatact ggttcatgga tgatggtggt aaatgggatt acaacaaaaa
     4381 ctctaccaac aaatcgatcg tactgaacac ccagtctttc actttcgaag aagtagaata
     4441 cctggttaag ggtctgcgta acaaattcca actgaactgt tacgtaaaaa tcaacaaaaa
     4501 caaaccgatc atctacatcg attctatgtc ttacctgatc ttctacaacc tgatcaaacc
     4561 gtacctgatc ccgcagatga tgtacaaact gccgaacact atctcctccg aaactttcct
     4621 gaaataatag ggataacagg gtaatggatc cctgcaggcc tcagggcccg atcgatgcgg
     4681 ccgcttaatt aattaatcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     4741 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     4801 ccctagacct agtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4861 accccgtaga aaagatcaaa ggatcttc
//
