LOCUS       sequence-486122-        9667 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Lentiviral eBAR2 backbone plasmid to insert epegRNAs (tevopreq1) of 
            interest with puromycin resistance. The cloning site is BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Niu X, Tang W, Liu Y, Mo B, Yu Y, Liu Y, Wei W
  TITLE     Prime editor-based high-throughput screening reveals functional 
            synonymous mutations in human cells.
  JOURNAL   Nat Biotechnol. 2025 Jun 24. doi: 10.1038/s41587-025-02710-z.
  PUBMED    40555761
REFERENCE   2  (bases 1 to 9667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Jun 24. doi: 10.1038/s41587-025-02710-z."
COMMENT     Sequence Label: pLenti-U6-epegRNA eBAR2-SV40-puro
FEATURES             Location/Qualifiers
     source          1..9667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2641..2889
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2641..2661
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2812..2831
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     2913..2932
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2913..2931
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2961..3085
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3514..3819)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3616..3635)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4139..4822)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     4737..4756
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4823..4925)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(5103..5227)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     5418..5434
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5476..5509)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5565..5894
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5745..5880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5901..6500
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5901..5920)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6397..6417
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6556..7144
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6609..6629)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7027..7038)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7147..7163)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7673..7853
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7915..8503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7995..8014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8674..9465,9466..9534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9297..9316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9535..9639)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag gtaccaaggt ctggcaggaa
     2641 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     2701 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     2761 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     2821 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     2881 cgaaacaccg agagacgctg gcttatcgaa attaatacga ctcactatag ggagacccaa
     2941 gctggctagt taagctatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     3001 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     3061 aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt gacctgtagt
     3121 cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg accgacagcc
     3181 ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat
     3241 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac
     3301 aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca
     3361 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga
     3421 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc
     3481 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
     3541 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
     3601 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
     3661 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
     3721 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
     3781 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
     3841 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
     3901 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
     3961 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
     4021 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
     4081 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
     4141 agtaagccgg atccacgcgg cgtttacgcc ccccctgcca ctcatcgcag tactgttgta
     4201 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     4261 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     4321 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     4381 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     4441 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     4501 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     4561 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     4621 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     4681 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     4741 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     4801 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     4861 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     4921 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     4981 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     5041 cgtcgggtga tgctgccaac ttagtcgact acaggtcact aataccatct aagtagttga
     5101 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     5161 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     5221 aagtggttga tctagagggc ccgcggttcg aacgtctctg tttaagagct atgctggact
     5281 cgctaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     5341 cgagtcggtg cttttttttc cgtctctcgc ggttctatct agttacgcgt taaaccaact
     5401 agaatttttt tgcagctcga ggtcgacggt atcgataagc tcgcttcacg agattccagc
     5461 aggtcgaggg acctaataac ttcgtatagc atacattata cgaagttata ttaagggttc
     5521 caagcttaag cggccgcgtg gataaccgta ttaccgccat gcaggtgtgt cagttagggt
     5581 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5641 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5701 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5821 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5881 taggcttttg caaagctagc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     5941 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     6001 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     6061 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     6121 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     6181 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     6241 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc
     6301 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6361 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6421 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     6481 cccgcaagcc cggtgcctga acatgtttaa gggttccggt tccactaggt acaattcgat
     6541 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6601 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6661 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6721 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6781 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6841 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6901 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6961 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7021 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7081 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7141 ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag
     7201 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     7261 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     7321 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7381 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     7441 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     7501 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     7561 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     7621 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     7681 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7741 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7801 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg
     7861 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8041 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8101 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8341 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8581 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8641 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8701 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8761 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8821 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8881 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8941 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9001 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9061 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9121 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9181 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9241 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9301 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9361 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9421 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9481 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9541 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9601 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9661 acctgac
//
