LOCUS       sequence-486152-        3218 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Ae aegypti UbL40 promoter expressing barstar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Nevard K, Ang JXD, Anderson MAE, Gonzalez E, Shackleford L, Alphey L
  TITLE     Harnessing the highly adaptable barnase-barstar system for genetic 
            biocontrol of Aedes aegypti.
  JOURNAL   Commun Biol. 2025 Aug 4;8(1):1154. doi: 10.1038/s42003-025-08588-6.
  PUBMED    40760041
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2025-08-4"; volume: "8(1):1154. doi"; pages: " 
            10.1038/s42003-025-08588-6"
COMMENT     Sequence Label: AGG1984 UbL40-Bs
FEATURES             Location/Qualifiers
     source          1..3218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(191..215)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(315..1106,1107..1175))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     938..957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1176..1280)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1379..1401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1393..1410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1394..1410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2154..2219
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2338..2459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2375..2394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2429..2448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2516..2532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2516..2532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2529..2551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(join(2704..3218,1..74))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2784..2803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
       61 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct ctattcaaca
      121 aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg tcctcgtgat
      181 accaattcgg agcctgcttt tttgtacaaa cttgttgata atggcaattc aaggatcttc
      241 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      301 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      361 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
      421 ttaccatctg gccccagtgc tgcaatgata ccgcgagagc cacgctcacc ggctccagat
      481 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
      541 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      601 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      661 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      721 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
      781 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
      841 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
      901 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
      961 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1021 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1081 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1141 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1201 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1261 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cagatacctg aaacaaaacc
     1321 catcgtacgg ccaaggaagt ctccaataac tgtgatccac cacaagcgcc agggttttcc
     1381 cagtcacgac gttgtaaaac gacggccagt catgcataat ccgcacgcat ctggaataag
     1441 gaagtgccat tccgcctgac ctcttgcatg cgtgcacaga atatgattgc atttcatgac
     1501 cgggtatccg ttcggcgcag gccgctgaac tgaaattcag ttttgacagc tccactgact
     1561 tcaagctctt tttcaccgaa acacatctcg gaagagacac gttgggaaag agtaagtttg
     1621 atttttgccg tttttcgtgg atttttagtg ataattcgtg gtttttttat gctagcaacc
     1681 taaattattg atagctaaag ttagaaattg tgaaatattg ttgaaattcc cacattattc
     1741 gtagtttatt cgatttgttg tagttgatgc attctaacct caatgtggtt tcgtctccgt
     1801 ccaattccga tgttattgtc cagttcgtta atttttggtg ttttctcccc gacttttcca
     1861 cagactgatc caagatgaag aaggccgtga tcaacggaga acagatccgt tcgatctcgg
     1921 atctgcacca gaccctgaag aaggaactgg ccctgccgga atactacgga gaaaacctgg
     1981 atgccctgtg ggattgcctg accggatggg tggaataccc gctggtgctg gaatggcggc
     2041 agttcgaaca gtcgaagcag ctgaccgaaa acggagccga atcggtgctg caggtgttcc
     2101 gtgaagccaa ggccgaagga gccgatatca ccatcatcct gtcgggtgcc ggagactata
     2161 aggaccacga cggagactac aaggatcatg atattgatta caaagacgat gacgataagt
     2221 aactagctga tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2281 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2341 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2401 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     2461 ggctaggtgg aggctcagtg atgataagtc tgcgatggtg gatgcatgtg tcatggtcat
     2521 agctgtttcc tgtgtgaaat tgttatccgc tcagagggca caatcctatt ccgcgctatc
     2581 cgacaatctc caagacatta ggtggagttc agttcggcgt atggcatatg tcgctggaaa
     2641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2701 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2761 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2881 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3121 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3181 taccttcgga aaaagagttg gtagctcttg atccggca
//
