LOCUS       sequence-486159-        7389 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (Alpha) protein for creating pseudotyped 
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (Alpha)
FEATURES             Location/Qualifiers
     source          1..7389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3785..4009
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4424..5012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4504..4523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5183..5974,5975..6043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5806..5825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6044..6148)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6331..6710
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6711..6914
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6865..6885
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6911..6935
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtgt ttctggttct tcttcccctg gtgtctagtc agtgcgtcaa cttgactacg
       61 cgaacacagc tgcccccagc ctacaccaat tcctttacac gaggcgtcta ctatcccgac
      121 aaggtgttcc gaagcagtgt tttgcactcc acgcaggacc ttttcttgcc cttcttctca
      181 aatgtgactt ggtttcatgc aatatccggg actaatggca ctaagagatt cgacaatccc
      241 gtgttgcctt ttaacgacgg cgtgtatttt gccagtacag aaaagagcaa tattatccgg
      301 ggatggatct tcggcacgac tctggatagt aaaacacaat ccctgctgat cgtgaataat
      361 gcgacaaacg tcgtcataaa agtctgcgag ttccagttct gcaacgaccc tttcctcggg
      421 gtgtaccata agaacaataa atcttggatg gaaagtgagt ttcgcgtgta ctccagtgca
      481 aataactgta cttttgagta cgtaagccag cctttcctga tggacctcga agggaaacag
      541 gggaacttta agaacctgcg cgaattcgtg ttcaagaaca tcgacggtta ttttaaaata
      601 tacagcaagc acacccctat taacctggtg agagatttgc cacaaggttt tagcgccctg
      661 gaacccctcg tggaccttcc tatcggcatc aacatcacgc gcttccaaac tctgctcgca
      721 ctgcatcggt cttatctgac ccctggagat tcatcctcag gatggaccgc gggtgctgct
      781 gcatattatg tgggatatct gcagcctagg accttcctgc tcaaatacaa cgagaacggc
      841 accatcactg acgctgttga ttgcgcgctg gacccattgt ctgaaaccaa atgcacactg
      901 aaaagcttca cagtcgagaa gggcatttac cagacgagca acttcagagt tcagcccaca
      961 gagagcatcg ttcgcttccc caacattacc aatttgtgcc cattcggaga ggtctttaac
     1021 gcaactagat ttgccagtgt ttacgcctgg aacaggaaac ggatatccaa ttgcgtggcc
     1081 gattacagcg tactgtacaa tagtgctagc ttttcaacct ttaaatgcta cggtgtctcc
     1141 cctacgaagc tgaatgatct gtgctttact aatgtctatg cggacagctt cgttattaga
     1201 ggtgacgagg tccgccaaat cgccccaggg cagactggaa agatagccga ctacaattac
     1261 aaactgccag atgatttcac cggctgcgtc atcgcctgga actccaataa cctcgatagt
     1321 aaagtgggcg gcaattacaa ctatctctat aggctgtttc gaaagagcaa cctgaaacct
     1381 tttgagcggg acatttcaac agagatctac caggccggaa gcaccccctg caacggggtc
     1441 gaggggttca attgttactt cccgctgcaa agctatggat ttcagccgac ttacggcgtt
     1501 ggctatcagc catatcgggt ggtggtgctg tcatttgagc tgcttcatgc gcctgctaca
     1561 gtatgtgggc ctaaaaagtc taccaatctg gtgaaaaata agtgcgtgaa cttcaacttc
     1621 aatggcctca ccgggacagg cgtgttgaca gaatccaata agaaattcct gccattccag
     1681 cagttcggca gggatatcga cgacacgacc gatgcggtgc gagatcccca gacattggag
     1741 attctggaca ttactccttg ttcttttgga ggagtgagtg ttattactcc cggcacaaat
     1801 acctcaaatc aagtcgctgt gctgtatcaa ggtgtaaact gtaccgaggt ccccgtggct
     1861 attcacgccg accagctgac cccaacatgg cgggtataca gcaccggttc taatgtattc
     1921 cagaccagag ccggctgcct cattggagcc gagcatgtga acaactctta tgaatgtgat
     1981 ataccgattg gagcaggtat ctgcgcctct tatcagaccc agacaaatag ccacagaagg
     2041 gctaggagcg tggcctccca gtccataatc gcctatacaa tgtcactggg ggctgaaaat
     2101 tcagtggcat acagtaacaa ctctatcgct atccccataa attttaccat ctctgtgact
     2161 acagagatcc tgccagtgtc tatgactaaa acctccgtgg actgtaccat gtacatctgt
     2221 ggtgatagca ccgaatgttc caacctgctc ctgcagtacg gcagcttctg cacacagctg
     2281 aatagggcac tgacaggaat cgccgtcgaa caggataaga acactcagga ggtgtttgcg
     2341 caggttaagc agatttacaa gacgccacca atcaaggact tcggtgggtt caatttctct
     2401 caaattctgc ctgatccttc taagccatct aagcggagct ttatagagga tttgctcttt
     2461 aataaggtga ccctcgccga tgctggattt attaagcagt atggcgactg tttgggagac
     2521 attgctgctc gcgatctgat atgtgcccag aaattcaacg ggctgaccgt actcccaccg
     2581 ctgctcactg atgagatgat cgcacaatat accagcgccc tgctggccgg cactattaca
     2641 tccggctgga cgttcggagc aggagccgca ctgcaaatac cttttgccat gcagatggca
     2701 tatcgcttca atgggatcgg ggtgacccag aacgtcctgt acgaaaacca gaaacttatc
     2761 gctaaccagt tcaattccgc cattggtaaa atccaggaca gcttgagttc cacagccagc
     2821 gccctcggga aattgcaaga tgtagtcaat cagaatgcac aagccctgaa tactctcgtc
     2881 aagcaactgt catccaactt cggtgctata tcctccgtgc tgaatgatat ccttgctaga
     2941 ttggataagg tggaggcaga agtccagatc gaccggctta ttactggaag gctgcagagt
     3001 ctgcagacgt acgtcacgca gcagcttatc agagccgccg agattagagc atctgcgaat
     3061 ctggctgcta caaagatgag tgaatgcgtg ctcgggcaat caaagcgggt cgacttttgt
     3121 gggaaggggt accacctcat gagcttcccc caatccgccc cacatggcgt tgtgttcctg
     3181 catgttactt acgttcccgc tcaagagaag aactttacta ccgctccggc gatctgccac
     3241 gacggtaagg cacactttcc acgcgaaggc gtctttgtta gcaatggaac acactggttt
     3301 gttacccaga ggaactttta tgagccccag ataatcacaa cccacaacac attcgtatct
     3361 gggaactgtg acgtagttat tgggatcgtg aacaacaccg tgtacgatcc tctccaaccg
     3421 gaacttgact ctttcaagga ggaactggat aagtacttta agaatcacac ctcacccgat
     3481 gttgacctcg gcgacatttc aggaattaat gctagcgtgg tgaacatcca gaaagagatt
     3541 gatagactta acgaagtggc caagaacctc aacgaatcac ttattgacct gcaggagctg
     3601 ggaaaatatg agcagtacat taaatggcct tggtacattt ggctgggctt tatagcaggc
     3661 ctgatcgcta tcgtcatggt gaccatcatg ctttgttgta tgactagctg ctgcagctgc
     3721 ctcaagggct gttgtagctg cggctcttgc tgtaaataat gatgatgatg aggatccaga
     3781 tctgctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3841 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3901 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3961 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg tacccaggtg
     4021 ctgaagaatt gacccggttc ctcctgggcc agaaagaagc aggcacatcc ccttctctgt
     4081 gacacaccct gtccacgccc ctggttctta gttccagccc cactcatagg acactcatag
     4141 ctcaggaggg ctccgccttc aatcccaccc gctaaagtac ttggagcggt ctctccctcc
     4201 ctcatcagcc caccaaacca aacctagcct ccaagagtgg gaagaaatta aagcaagata
     4261 ggctattaag tgcagaggga gagaaaatgc ctccaacatg tgaggaagta atgagagaaa
     4321 tcatagaatt ttaaggccat gatttaaggc caatcggaaa gaacatgtga gcaaaaggcc
     4381 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4441 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4501 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4561 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4621 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4681 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4741 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4801 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4861 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4921 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4981 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5041 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5101 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5161 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5221 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5281 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5341 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5401 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5461 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5521 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5581 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5641 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5701 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5761 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5821 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5881 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5941 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6001 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6061 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6121 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcc
     6181 caatgattgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     6241 atcagattgg ctattggcca ttgcatacgt tgtatccata tcataatatg tacatttata
     6301 ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt
     6361 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     6421 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     6481 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     6541 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     6601 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     6661 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     6721 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     6781 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     6841 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     6901 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     6961 ttgacctcca tagaagacac cgggaccgat ccagcctcca tcggctcgca tctctccttc
     7021 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     7081 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     7141 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     7201 gctttgcctg accctgcttg ctcaactcta gttaacggtg gagggcagtg tagtctgagc
     7261 agtactcgtt gctgccgcgc gcgccaccag acataatagc tgacagacta acagactgtt
     7321 cctttccatg ggtcttttct gcagtcaccg tcgtcgacac gtgtgatcag atatcgcggc
     7381 cgctctaga
//
