LOCUS       sequence-486169-        7392 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (B.1-4d) protein for creating pseudotyped
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (B.1-4d)
FEATURES             Location/Qualifiers
     source          1..7392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3788..4012
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4427..5015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4507..4526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5186..5977,5978..6046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5809..5828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6047..6151)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6334..6713
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6714..6917
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6868..6888
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6914..6938
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtgt ttctggttct tcttcccctg gtgtctagtc agtgcgtcaa cttgactacg
       61 cgaacacagc tgcccccagc ctacaccaat tcctttacac gaggcgtcta ctatcccgac
      121 aaggtgttcc gaagcagtgt tttgcactcc acgcaggacc ttttcttgcc cttcttctca
      181 aatgtgactt ggtttcatgc aatacacgta tccgggacta atggcatcaa gagattcgac
      241 aatcccgtgt tgccttttaa cgacggcgtg tattttgcca gtacagaaaa gagcaatatt
      301 atccggggat ggatcttcgg cacgactctg gatagtaaaa cacaatccct gctgatcgtg
      361 aataatgcga caaacgtcgt cataaaagtc tgcgagttcc agttctgcaa cgaccctttc
      421 ctcggggtgt attaccataa gaacaataaa tcttggagcg aaagtgagtt tcgcgtgtac
      481 tccagtgcaa ataactgtac ttttgagtac gtaagccagc ctttcctgat ggacctcgaa
      541 gggaaacagg ggaactttaa gaacctgcgc gaattcgtgt tcaagaacat cgacggttat
      601 tttaaaatat acagcaagca cacccctatt aacctggtga gagatttgcc acaaggtttt
      661 agcgccctgg aacccctcgt ggaccttcct atcggcatca acatcacgcg cttccaaact
      721 ctgctcgcac tgcatcggtc ttatctgacc cctggagatt catcctcagg atggaccgcg
      781 ggtgctgctg catattatgt gggatatctg cagcctagga ccttcctgct caaatacaac
      841 gagaacggca ccatcactga cgctgttgat tgcgcgctgg acccattgtc tgaaaccaaa
      901 tgcacactga aaagcttcac agtcgagaag ggcatttacc agacgagcaa cttcagagtt
      961 cagcccacag agagcatcgt tcgcttcccc aacattacca atttgtgccc attcggagag
     1021 gtctttaacg caactagatt tgccagtgtt tacgcctgga acaggaaacg gatatccaat
     1081 tgcgtggccg attacagcgt actgtacaat agtgctagct tttcaacctt taaatgctac
     1141 ggtgtctccc ctacgaagct gaatgatctg tgctttacta atgtctatgc ggacagcttc
     1201 gttattagag gtgacgaggt ccgccaaatc gccccagggc agactggaaa gatagccgac
     1261 tacaattaca aactgccaga tgatttcacc ggctgcgtca tcgcctggaa ctccaataac
     1321 ctcgatagta aagtgggcgg caattacaac tatctctata ggctgtttcg aaagagcaac
     1381 ctgaaacctt ttgagcggga catttcaaca gagatcttcc aggccggaag caccccctgc
     1441 aacggggtcg aggggttcaa ttgttacttc ccgctgcgga gctatggatt tcagccgact
     1501 aacggcgttg gctatcagcc atatcgggtg gtggtgctgt catttgagct gcttcatgcg
     1561 cctgctacag tatgtgggcc taaaaagtct accaatctgg tgaaaaataa gtgcgtgaac
     1621 ttcaacttca atggcctcac cgggacaggc gtgttgacag aatccaataa gaaattcctg
     1681 ccattccagc agttcggcag ggatatcgca gacacgaccg atgcggtgcg agatccccag
     1741 acattggaga ttctggacat tactccttgt tcttttggag gagtgagtgt tattactccc
     1801 ggcacaaata cctcaaatca agtcgctgtg ctgtatcaag gtgtaaactg taccgaggtc
     1861 cccgtggcta ttcacgccga ccagctgacc ccaacatggc gggtatacag caccggttct
     1921 aatgtattcc agaccagagc cggctgcctc attggagccg agcatgtgaa caactcttat
     1981 gaatgtgata taccgattgg agcaggtatc tgcgcctctt atcagaccca gacaaatagc
     2041 cccagaaggg ctaggagcgt ggcctcccag tccataatcg cctatacaat gtcactgggg
     2101 gctgaaaatt cagtggcata cagtaacaac tctatcgcta tccccacaaa ttttaccatc
     2161 tctgtgacta cagagatcct gccagtgtct atgactaaaa cctccgtgga ctgtaccatg
     2221 tacatctgtg gtgatagcac cgaatgttcc aacctgctcc tgcagtacgg cagcttctgc
     2281 acacagctga atagggcact gacaggaatc gccgtcgaac aggataagaa cactcaggag
     2341 gtgtttgcgc aggttaagca gatttacaag acgccaccaa tcaaggactt cggtgggttc
     2401 aatttctctc aaattctgcc tgatccttct aagccatcta agcggagctt tatagaggat
     2461 ttgctcttta ataaggtgac cctcgccgat gctggattta ttaagcagta tggcgactgt
     2521 ttgggagaca ttgctgctcg cgatctgata tgtgcccaga aattcaacgg gctgaccgta
     2581 ctcccaccgc tgctcactga tgagatgatc gcacaatata ccagcgccct gctggccggc
     2641 actattacat ccggctggac gttcggagca ggagccgcac tgcaaatacc ttttgccatg
     2701 cagatggcat atcgcttcaa tgggatcggg gtgacccaga acgtcctgta cgaaaaccag
     2761 aaacttatcg ctaaccagtt caattccgcc attggtaaaa tccaggacag cttgagttcc
     2821 acagccagcg ccctcgggaa attgcaagat gtagtcaatc agaatgcaca agccctgaat
     2881 actctcgtca agcaactgtc atccaacttc ggtgctatat cctccgtgct gaatgatatc
     2941 cttagtagat tggataaggt ggaggcagaa gtccagatcg accggcttat tactggaagg
     3001 ctgcagagtc tgcagacgta cgtcacgcag cagcttatca gagccgccga gattagagca
     3061 tctgcgaatc tggctgctac aaagatgagt gaatgcgtgc tcgggcaatc aaagcgggtc
     3121 gacttttgtg ggaaggggta ccacctcatg agcttccccc aatccgcccc acatggcgtt
     3181 gtgttcctgc atgttactta cgttcccgct caagagaaga actttactac cgctccggcg
     3241 atctgccacg acggtaaggc acactttcca cgcgaaggcg tctttgttag caatggaaca
     3301 cactggtttg ttacccagag gaacttttat gagccccaga taatcacaac cgacaacaca
     3361 ttcgtatctg ggaactgtga cgtagttatt gggatcgtga acaacaccgt gtacgatcct
     3421 ctccaaccgg aacttgactc tttcaaggag gaactggata agtactttaa gaatcacacc
     3481 tcacccgatg ttgacctcgg cgacatttca ggaattaatg ctagcgtggt gaacatccag
     3541 aaagagattg atagacttaa cgaagtggcc aagaacctca acgaatcact tattgacctg
     3601 caggagctgg gaaaatatga gcagtacatt aaatggcctt ggtacatttg gctgggcttt
     3661 atagcaggcc tgatcgctat cgtcatggtg accatcatgc tttgttgtat gactagctgc
     3721 tgcagctgcc tcaagggctg ttgtagctgc ggctcttgct gtaaataatg atgaggatcc
     3781 agatctgctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3841 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3901 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3961 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat gggtacccag
     4021 gtgctgaaga attgacccgg ttcctcctgg gccagaaaga agcaggcaca tccccttctc
     4081 tgtgacacac cctgtccacg cccctggttc ttagttccag ccccactcat aggacactca
     4141 tagctcagga gggctccgcc ttcaatccca cccgctaaag tacttggagc ggtctctccc
     4201 tccctcatca gcccaccaaa ccaaacctag cctccaagag tgggaagaaa ttaaagcaag
     4261 ataggctatt aagtgcagag ggagagaaaa tgcctccaac atgtgaggaa gtaatgagag
     4321 aaatcataga attttaaggc catgatttaa ggccaatcgg aaagaacatg tgagcaaaag
     4381 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4441 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4501 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4561 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4621 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4681 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4741 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4801 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4861 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4921 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4981 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5041 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5101 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5161 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5221 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5281 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5341 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5401 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5461 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5521 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5581 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5641 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5701 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5761 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5821 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5881 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5941 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6001 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6061 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6121 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     6181 tcccaatgat tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6241 cgcatcagat tggctattgg ccattgcata cgttgtatcc atatcataat atgtacattt
     6301 atattggctc atgtccaaca ttaccgccat gttgacattg attattgact agttattaat
     6361 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     6421 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     6481 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     6541 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     6601 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     6661 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     6721 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     6781 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     6841 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     6901 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     6961 gttttgacct ccatagaaga caccgggacc gatccagcct ccatcggctc gcatctctcc
     7021 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     7081 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     7141 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     7201 cacgctttgc ctgaccctgc ttgctcaact ctagttaacg gtggagggca gtgtagtctg
     7261 agcagtactc gttgctgccg cgcgcgccac cagacataat agctgacaga ctaacagact
     7321 gttcctttcc atgggtcttt tctgcagtca ccgtcgtcga cacgtgtgat cagatatcgc
     7381 ggccgctcta ga
//
