LOCUS       sequence-486181-        7383 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (BA.2) protein for creating pseudotyped 
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (BA.2)
FEATURES             Location/Qualifiers
     source          1..7383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(313..324)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3779..4003
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4418..5006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4498..4517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5177..5968,5969..6037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5800..5819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6038..6142)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6325..6704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6705..6908
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6859..6879
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6905..6929
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtat ttctggttct gcttcccctc gtgtctagtc agtgcgtcaa cttgatcacc
       61 cgaacacagt cctacactaa ttcctttaca aggggcgtgt actaccccga caaagtattt
      121 cgaagtagcg ttttgcactc aacccaggac ctgttccttc ccttcttttc aaacgtcaca
      181 tggtttcacg ccatccatgt ctcagggacc aatggtacaa aaaggttcga caaccccgtg
      241 ttgcctttca acgatggcgt ctacttcgcc agtaccgaaa agagcaatat catacgggga
      301 tggatttttg gcacgaccct cgatagtaaa acacaaagtc tgctcattgt gaataatgcc
      361 acaaacgtcg tcatcaaggt gtgtgagttt cagttttgca acgacccgtt cctggatgtg
      421 tattaccata aaaataataa atcttggatg gaaagtgagt tccgagtgta ctccagcgca
      481 aataactgta cctttgagta tgtgtcacag ccgttcctca tggacctcga aggcaaacag
      541 ggaaacttca agaacctgcg ggaattcgtc ttcaaaaaca ttgacggtta tttcaagatt
      601 tacagcaaac atacccctat caacctgggc agagatctgc cgcaaggttt ttccgccctg
      661 gaacctctcg tcgacctgcc tatcggtatc aacatcaccc gcttccaaac gctgctcgca
      721 ctccatcggt cttatctgac tcctggagac tcatccagcg gatggaccgc cggcgccgct
      781 gcatattacg ttggttatct gcagcctagg acctttctgc ttaagtacaa cgagaatggc
      841 actattaccg acgctgttga ttgcgcactc gacccactct ctgaaaccaa atgcacgctg
      901 aaaagcttca cagtcgagaa ggggatttac cagacttcta acttcagggt tcagcccact
      961 gagagcattg ttcgcttccc caacatcacc aacttgtgcc ccttcgacga ggtattcaat
     1021 gccaccaggt tcgcatccgt gtacgcctgg aataggaaga gaataagcaa ttgcgtagcc
     1081 gattattcag tcctttataa tttcgcccca tttttcgctt tcaaatgcta cggcgtttcc
     1141 cccacgaagc tgaacgatct ttgctttact aacgtgtacg ccgactcctt cgtaatcaga
     1201 gggaacgagg tgagccaaat agctcccggt cagactggaa atattgcaga ctataattat
     1261 aaactgccgg atgatttcac tggttgtgtg attgcctgga actccaacaa actcgatagc
     1321 aaagtgggcg ggaattataa ctacctctac aggctgtttc gaaagagtaa cttgaagcct
     1381 tttgagcgcg atattagtac tgagatctac caggcaggaa ataagccctg caatggggtc
     1441 gcgggcttca attgttattt tccactgaga agttacggat ttcgcccaac atacggggtt
     1501 ggtcaccagc cataccgggt ggtggtcctg tcattcgagc tgcttcatgc ccctgctaca
     1561 gtgtgtgggc ctaaaaagtc tacgaacctg gttaaaaata agtgcgtgaa cttcaacttt
     1621 aacggcctca ctggcaccgg ggtgcttaca gaatccaaca agaaattcct gccttttcag
     1681 cagtttggca gggatatcgc agacaccacc gacgcagtga gagatcccca gacattggag
     1741 attctggaca taactccatg ttcttttggc ggagtctccg ttattactcc cggtacaaac
     1801 actagcaatc aagtggctgt gctgtaccag ggggtaaact gcaccgaggt ccccgtggct
     1861 attcacgccg accagctgac tccaacatgg cgcgtgtaca gtactggcag taatgtgttt
     1921 cagacccggg ccgggtgcct cattggagca gagtacgtca acaactctta tgaatgtgat
     1981 atccctatcg gagcaggcat ttgcgcctct tatcaaaccc agacaaaatc acacaggaga
     2041 gctagaagcg tggcgtccca gtccatcatc gcatatacaa tgtctctggg ggctgaaaac
     2101 tcagtagctt atagcaacaa ttctattgct atccccacaa attttactat ctctgtcact
     2161 acagagatac tgccagtgtc tatgacaaaa actagcgtgg actgtaccat gtacatatgc
     2221 ggagatagca ccgaatgctc caatctgctc cttcagtacg gcagcttctg cacacagctg
     2281 aagcgagccc tgacgggaat cgccgtcgaa caggataaga acacccagga ggtttttgcc
     2341 caggtcaaac agatctataa gacgccacca ataaagtact tcggtgggtt caatttttct
     2401 caaatcctcc ctgatccttc taagccttca aagcggagct ttatagagga tctgctcttt
     2461 aataaggtga ccctcgccga tgctggattt atcaagcagt atggcgattg tctcggggac
     2521 attgctgctc gggatctgat ctgtgcccag aagttcaatg gcctgaccgt actgccaccg
     2581 ctgctgaccg atgagatgat cgcgcaatac accagcgcac ttctggcagg aactattaca
     2641 tccggctgga cattcggggc gggagcggca ctgcaaattc cttttgcaat gcagatggcc
     2701 tatcggttca atggaatcgg cgtgacccag aacgttcttt atgaaaatca gaagctgatt
     2761 gcgaaccagt tcaattccgc cattggcaaa atacaggaca gccttagctc cacagcctcc
     2821 gctctcggga agttgcaaga cgtggtcaat cacaacgccc aagcgctgaa cactttggtg
     2881 aagcaactga gctcaaaatt cggcgccata agttccgtac tgaatgacat ccttagtcgc
     2941 ctggataagg tggaggccga agtgcagatc gaccggttga ttaccggaag actgcagagc
     3001 ttgcagactt acgtcacaca gcaattgatt agagcggccg agattagagc gtctgccaat
     3061 ctggctgcta caaaaatgag cgaatgcgtt cttgggcagt caaagcgcgt ggacttttgt
     3121 ggcaagggct accacctgat gtcattccct caatccgccc cacacggtgt tgtttttctg
     3181 cacgttacct acgtgcccgc tcaagaaaag aactttacca cggcaccagc catctgccac
     3241 gacggtaagg ctcattttcc aagagaaggt gtcttcgtga gtaatggaac acattggttc
     3301 gtgacgcagc gcaatttcta cgagccccag ataattacaa ccgacaacac tttcgtatct
     3361 ggcaattgtg acgtggttat tgggatcgtg aacaataccg tgtatgaccc cctgcaaccg
     3421 gaactggact cttttaagga ggaactggat aagtatttta aaaatcatac ctcacccgat
     3481 gtagatctgg gggatatctc aggaattaac gctagcgtgg tcaacatcca gaaagagatc
     3541 gacagactga acgaagtggc aaagaacctt aacgagagcc tgatagacct gcaggagctg
     3601 ggaaaatacg agcagtatat taagtggcca tggtatatat ggctgggctt tatcgccggg
     3661 ctcatcgcta ttgtgatggt cactatcatg ttgtgttgca tgacgtcctg ctgtagttgt
     3721 ttgaagggct gttgttcctg cgggtcttgc tgtaaataat gatgaggatc cagatctgct
     3781 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3841 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3901 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3961 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggtaccca ggtgctgaag
     4021 aattgacccg gttcctcctg ggccagaaag aagcaggcac atccccttct ctgtgacaca
     4081 ccctgtccac gcccctggtt cttagttcca gccccactca taggacactc atagctcagg
     4141 agggctccgc cttcaatccc acccgctaaa gtacttggag cggtctctcc ctccctcatc
     4201 agcccaccaa accaaaccta gcctccaaga gtgggaagaa attaaagcaa gataggctat
     4261 taagtgcaga gggagagaaa atgcctccaa catgtgagga agtaatgaga gaaatcatag
     4321 aattttaagg ccatgattta aggccaatcg gaaagaacat gtgagcaaaa ggccagcaaa
     4381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcccaatga
     6181 ttgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcaga
     6241 ttggctattg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
     6301 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
     6361 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     6421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     6481 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     6541 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     6601 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     6661 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     6721 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     6781 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     6841 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     6901 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     6961 tccatagaag acaccgggac cgatccagcc tccatcggct cgcatctctc cttcacgcgc
     7021 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     7081 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     7141 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     7201 cctgaccctg cttgctcaac tctagttaac ggtggagggc agtgtagtct gagcagtact
     7261 cgttgctgcc gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc
     7321 catgggtctt ttctgcagtc accgtcgtcg acacgtgtga tcagatatcg cggccgctct
     7381 aga
//
