LOCUS       sequence-486190-        7386 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (BA.2.12.1-4a) protein for creating 
            pseudotyped virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-4a)
FEATURES             Location/Qualifiers
     source          1..7386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3782..4006
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4421..5009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4501..4520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5180..5971,5972..6040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5803..5822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6041..6145)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6328..6707
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6708..6911
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6862..6882
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6908..6932
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtgt ttctggttct tcttcccctg gtgtctagtc agtgcgtcaa cttgatcacg
       61 cgaacacagc tgagctacac caattccttt acacgaggcg tctactatcc cgacaaggtg
      121 ttccgaagca gtgttttgca ctccacgcag gaccttttct tgcccttctt ctcaaatgtg
      181 acttggtttc atgcaataca cgtatccggg actaatggca tcaagagatt cgacaatccc
      241 gtgttgcctt ttaacgacgg cgtgtatttt gccagtacag aaaagagcaa tattatccgg
      301 ggatggatct tcggcacgac tctggatagt aaaacacaat ccctgctgat cgtgaataat
      361 gcgacaaacg tcgtcataaa agtctgcgag ttccagttct gcaacgaccc tttcctcgac
      421 gtgtattacc ataagaacaa taaatcttgg agcgaaagtg agtttcgcgt gtactccagt
      481 gcaaataact gtacttttga gtacgtaagc cagcctttcc tgatggacct cgaagggaaa
      541 caggggaact ttaagaacct gcgcgaattc gtgttcaaga acatcgacgg ttattttaaa
      601 atatacagca agcacacccc tattaacctg ggcagagatt tgccacaagg ttttagcgcc
      661 ctggaacccc tcgtggacct tcctatcggc atcaacatca cgcgcttcca aactctgctc
      721 gcactgcatc ggtcttatct gacccctgga gattcatcct caggatggac cgcgggtgct
      781 gctgcatatt atgtgggata tctgcagcct aggaccttcc tgctcaaata caacgagaac
      841 ggcaccatca ctgacgctgt tgattgcgcg ctggacccat tgtctgaaac caaatgcaca
      901 ctgaaaagct tcacagtcga gaagggcatt taccagacga gcaacttcag agttcagccc
      961 acagagagca tcgttcgctt ccccaacatt accaatttgt gcccattcga cgaggtcttt
     1021 aacgcaacta gatttgccag tgtttacgcc tggaacagga aacggatatc caattgcgtg
     1081 gccgattaca gcgtactgta caatttcgct ccctttttcg cctttaaatg ctacggtgtc
     1141 tcccctacga agctgaatga tctgtgcttt actaatgtct atgcggacag cttcgttatt
     1201 agaggtaacg aggtcagcca aatcgcccca gggcagactg gaaacatagc cgactacaat
     1261 tacaaactgc cagatgattt caccggctgc gtcatcgcct ggaactccaa taagctcgat
     1321 agtaaagtgg gcggcaatta caactatcag tataggctgt ttcgaaagag caacctgaaa
     1381 ccttttgagc gggacatttc aacagagatc ttccaggccg gaaacaagcc ctgcaacggg
     1441 gtcgccgggt tcaattgtta cttcccgctg cggagctatg gatttcggcc gacttacggc
     1501 gttggccacc agccatatcg ggtggtggtg ctgtcatttg agctgcttca tgcgcctgct
     1561 acagtatgtg ggcctaaaaa gtctaccaat ctggtgaaaa ataagtgcgt gaacttcaac
     1621 ttcaatggcc tcaccgggac aggcgtgttg acagaatcca ataagaaatt cctgccattc
     1681 cagcagttcg gcagggatat cgcagacacg accgatgcgg tgcgagatcc ccagacattg
     1741 gagattctgg acattactcc ttgttctttt ggaggagtga gtgttattac tcccggcaca
     1801 aatacctcaa atcaagtcgc tgtgctgtat caaggtgtaa actgtaccga ggtccccgtg
     1861 gctattcacg ccgaccagct gaccccaaca tggcgggtat acagcaccgg ttctaatgta
     1921 ttccagacca gagccggctg cctcattgga gccgagtacg tgaacaactc ttatgaatgt
     1981 gatataccga ttggagcagg tatctgcgcc tcttatcaga cccagacaaa gagccacaga
     2041 agggctagga gcgtggcctc ccagtccata atcgcctata caatgtcact gggggctgaa
     2101 aatctggtgg catacagtaa caactctatc gctatcccca caaattttac catctctgtg
     2161 actacagaga tcctgccagt gtctatgact aaaacctccg tggactgtac catgtacatc
     2221 tgtggtgata gcaccgaatg ttccaacctg ctcctgcagt acggcagctt ctgcacacag
     2281 ctgaagaggg cactgacagg aatcgccgtc gaacaggata agaacactca ggaggtgttt
     2341 gcgcaggtta agcagattta caagacgcca ccaatcaagt acttcggtgg gttcaatttc
     2401 tctcaaattc tgcctgatcc ttctaagcca tctaagcgga gctttataga ggatttgctc
     2461 tttaataagg tgaccctcgc cgatgctgga tttattaagc agtatggcga ctgtttggga
     2521 gacattgctg ctcgcgatct gatatgtgcc cagaaattca acgggctgac cgtactccca
     2581 ccgctgctca ctgatgagat gatcgcacaa tataccagcg ccctgctggc cggcactatt
     2641 acatccggct ggacgttcgg agcaggagcc gcactgcaaa taccttttgc catgcagatg
     2701 gcatatcgct tcaatgggat cggggtgacc cagaacgtcc tgtacgaaaa ccagaaactt
     2761 atcgctaacc agttcaattc cgccattggt aaaatccagg acagcttgag ttccacagcc
     2821 agcgccctcg ggaaattgca agatgtagtc aatcacaatg cacaagccct gaatactctc
     2881 gtcaagcaac tgtcatccaa gttcggtgct atatcctccg tgctgaatga tatccttagt
     2941 agattggata aggtggaggc agaagtccag atcgaccggc ttattactgg aaggctgcag
     3001 agtctgcaga cgtacgtcac gcagcagctt atcagagccg ccgagattag agcatctgcg
     3061 aatctggctg ctacaaagat gagtgaatgc gtgctcgggc aatcaaagcg ggtcgacttt
     3121 tgtgggaagg ggtaccacct catgagcttc ccccaatccg ccccacatgg cgttgtgttc
     3181 ctgcatgtta cttacgttcc cgctcaagag aagaacttta ctaccgctcc ggcgatctgc
     3241 cacgacggta aggcacactt tccacgcgaa ggcgtctttg ttagcaatgg aacacactgg
     3301 tttgttaccc agaggaactt ttatgagccc cagataatca caaccgacaa cacattcgta
     3361 tctgggaact gtgacgtagt tattgggatc gtgaacaaca ccgtgtacga tcctctccaa
     3421 ccggaacttg actctttcaa ggaggaactg gataagtact ttaagaatca cacctcaccc
     3481 gatgttgacc tcggcgacat ttcaggaatt aatgctagcg tggtgaacat ccagaaagag
     3541 attgatagac ttaacgaagt ggccaagaac ctcaacgaat cacttattga cctgcaggag
     3601 ctgggaaaat atgagcagta cattaaatgg ccttggtaca tttggctggg ctttatagca
     3661 ggcctgatcg ctatcgtcat ggtgaccatc atgctttgtt gtatgactag ctgctgcagc
     3721 tgcctcaagg gctgttgtag ctgcggctct tgctgtaaat aatgatgagg atccagatct
     3781 gctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3841 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3901 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3961 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatgggtac ccaggtgctg
     4021 aagaattgac ccggttcctc ctgggccaga aagaagcagg cacatcccct tctctgtgac
     4081 acaccctgtc cacgcccctg gttcttagtt ccagccccac tcataggaca ctcatagctc
     4141 aggagggctc cgccttcaat cccacccgct aaagtacttg gagcggtctc tccctccctc
     4201 atcagcccac caaaccaaac ctagcctcca agagtgggaa gaaattaaag caagataggc
     4261 tattaagtgc agagggagag aaaatgcctc caacatgtga ggaagtaatg agagaaatca
     4321 tagaatttta aggccatgat ttaaggccaa tcggaaagaa catgtgagca aaaggccagc
     4381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4861 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5101 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5161 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5461 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5761 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcccaa
     6181 tgattgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     6241 agattggcta ttggccattg catacgttgt atccatatca taatatgtac atttatattg
     6301 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
     6361 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     6421 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     6481 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     6541 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     6601 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     6661 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     6721 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6781 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6841 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6901 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     6961 acctccatag aagacaccgg gaccgatcca gcctccatcg gctcgcatct ctccttcacg
     7021 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     7081 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     7141 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     7201 ttgcctgacc ctgcttgctc aactctagtt aacggtggag ggcagtgtag tctgagcagt
     7261 actcgttgct gccgcgcgcg ccaccagaca taatagctga cagactaaca gactgttcct
     7321 ttccatgggt cttttctgca gtcaccgtcg tcgacacgtg tgatcagata tcgcggccgc
     7381 tctaga
//
