LOCUS       sequence-486191-        7383 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (BA.2.12.1-4b) protein for creating 
            pseudotyped virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-4b)
FEATURES             Location/Qualifiers
     source          1..7383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3779..4003
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4418..5006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4498..4517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5177..5968,5969..6037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5800..5819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6038..6142)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6325..6704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6705..6908
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6859..6879
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6905..6929
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtgt ttctggttct tcttcccctg gtgtctagtc agtgcgtcaa cttgatcacg
       61 cgaacacaga gctacaccaa ttcctttaca cgaggcgtct actatcccga caaggtgttc
      121 cgaagcagtg ttttgcactc cacgcaggac cttttcttgc ccttcttctc aaatgtgact
      181 tggtttcatg caatacacgt atccgggact aatggcatca agagattcga caatcccgtg
      241 ttgcctttta acgacggcgt gtattttgcc agtacagaaa agagcaatat tatccgggga
      301 tggatcttcg gcacgactct ggatagtaaa acacaatccc tgctgatcgt gaataatgcg
      361 acaaacgtcg tcataaaagt ctgcgagttc cagttctgca acgacccttt cctcgacgtg
      421 tattaccata agaacaataa atcttggagc gaaagtgagt ttcgcgtgta ctccagtgca
      481 aataactgta cttttgagta cgtaagccag cctttcctga tggacctcga agggaaacag
      541 gggaacttta agaacctgcg cgaattcgtg ttcaagaaca tcgacggtta ttttaaaata
      601 tacagcaagc acacccctat taacctgggc agagatttgc cacaaggttt tagcgccctg
      661 gaacccctcg tggaccttcc tatcggcatc aacatcacgc gcttccaaac tctgctcgca
      721 ctgcatcggt cttatctgac ccctggagat tcatcctcag gatggaccgc gggtgctgct
      781 gcatattatg tgggatatct gcagcctagg accttcctgc tcaaatacaa cgagaacggc
      841 accatcactg acgctgttga ttgcgcgctg gacccattgt ctgaaaccaa atgcacactg
      901 aaaagcttca cagtcgagaa gggcatttac cagacgagca acttcagagt tcagcccaca
      961 gagagcatcg ttcgcttccc caacattacc aatttgtgcc cattcgacga ggtctttaac
     1021 gcaactagat ttgccagtgt ttacgcctgg aacaggaaac ggatatccaa ttgcgtggcc
     1081 gattacagcg tactgtacaa tttcgctccc tttttcgcct ttaaatgcta cggtgtctcc
     1141 cctacgaagc tgaatgatct gtgctttact aatgtctatg cggacagctt cgttattaga
     1201 ggtaacgagg tcagccaaat cgccccaggg cagactggaa acatagccga ctacaattac
     1261 aaactgccag atgatttcac cggctgcgtc atcgcctgga actccaataa gctcgatagt
     1321 aaagtgggcg gcaattacaa ctatcagtat aggctgtttc gaaagagcaa cctgaaacct
     1381 tttgagcggg acatttcaac agagatcttc caggccggaa acaagccctg caccggggtc
     1441 gccgggttca attgttactt cccgctgcgg agctatggat ttcggccgac ttacggcgtt
     1501 ggccaccagc catatcgggt ggtggtgctg tcatttgagc tgcttcatgc gcctgctaca
     1561 gtatgtgggc ctaaaaagtc taccaatctg gtgaaaaata agtgcgtgaa cttcaacttc
     1621 aatggcctca ccgggacagg cgtgttgaca gaatccaata agaaattcct gccattccag
     1681 cagttcggca gggatatcgc agacacgacc gatgcggtgc gagatcccca gacattggag
     1741 attctggaca ttactccttg ttcttttgga ggagtgagtg ttattactcc cggcacaaat
     1801 acctcaaatc aagtcgctgt gctgtatcaa ggtgtaaact gtaccgaggt ccccgtggct
     1861 attcacgccg accagctgac cccaacatgg cgggtataca gcaccggttc taatgtattc
     1921 cagaccagag ccggctgcct cattggagcc gagtacgtga acaactctta tgaatgtgat
     1981 ataccgattg gagcaggtat ctgcgcctct tatcagaccc agacaaagag ccacagaagg
     2041 gctaggagcg tggcctccca gtccataatc gcctatacaa tgtcactggg ggctgaaaat
     2101 ctggtggcat acagtaacaa ctctatcgct atccccacaa attttaccat ctctgtgact
     2161 acagagatcc tgccagtgtc tatgactaaa acctccgtgg actgtaccat gtacatctgt
     2221 ggtgatagca ccgaatgttc caacctgctc ctgcagtacg gcagcttctg cacacagctg
     2281 aagagggcac tgacaggaat cgccgtcgaa caggataaga acactcagga ggtgtttgcg
     2341 caggttaagc agatttacaa gacgccacca atcaagtact tcggtgggtt caatttctct
     2401 caaattctgc ctgatccttc taagccatct aagcggagct ttatagagga tttgctcttt
     2461 aataaggtga ccctcgccga tgctggattt attaagcagt atggcgactg tttgggagac
     2521 attgctgctc gcgatctgat atgtgcccag aaattcaacg ggctgaccgt actcccaccg
     2581 ctgctcactg atgagatgat cgcacaatat accagcgccc tgctggccgg cactattaca
     2641 tccggctgga cgttcggagc aggagccgca ctgcaaatac cttttgccat gcagatggca
     2701 tatcgcttca atgggatcgg ggtgacccag aacgtcctgt acgaaaacca gaaacttatc
     2761 gctaaccagt tcaattccgc cattggtaaa atccaggaca gcttgagttc cacagccagc
     2821 gccctcggga aattgcaaga tgtagtcaat cacaatgcac aagccctgaa tactctcgtc
     2881 aagcaactgt catccaagtt cggtgctata tcctccgtgc tgaatgatat ccttagtaga
     2941 ttggataagg tggaggcaga agtccagatc gaccggctta ttactggaag gctgcagagt
     3001 ctgcagacgt acgtcacgca gcagcttatc agagccgccg agattagagc atctgcgaat
     3061 ctggctgcta caaagatgag tgaatgcgtg ctcgggcaat caaagcgggt cgacttttgt
     3121 gggaaggggt accacctcat gagcttcccc caatccgccc cacatggcgt tgtgttcctg
     3181 catgttactt acgttcccgc tcaagagaag aactttacta ccgctccggc gatctgccac
     3241 gacggtaagg cacactttcc acgcgaaggc gtctttgtta gcaatggaac acactggttt
     3301 gttacccaga ggaactttta tgagccccag ataatcacaa ccgacaacac attcgtatct
     3361 gggaactgtg acgtagttat tgggatcgtg aacaacaccg tgtacgatcc tctccaaccg
     3421 gaacttgact ctttcaagga ggaactggat aagtacttta agaatcacac ctcacccgat
     3481 gttgacctcg gcgacatttc aggaattaat gctagcgtgg tgaacatcca gaaagagatt
     3541 gatagactta acgaagtggc caagaacctc aacgaatcac ttattgacct gcaggagctg
     3601 ggaaaatatg agcagtacat taaatggcct tggtacattt ggctgggctt tatagcaggc
     3661 ctgatcgcta tcgtcatggt gaccatcatg ctttgttgta tgactagctg ctgcagctgc
     3721 ctcaagggct gttgtagctg cggctcttgc tgtaaataat gatgaggatc cagatctgct
     3781 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3841 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3901 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3961 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggtaccca ggtgctgaag
     4021 aattgacccg gttcctcctg ggccagaaag aagcaggcac atccccttct ctgtgacaca
     4081 ccctgtccac gcccctggtt cttagttcca gccccactca taggacactc atagctcagg
     4141 agggctccgc cttcaatccc acccgctaaa gtacttggag cggtctctcc ctccctcatc
     4201 agcccaccaa accaaaccta gcctccaaga gtgggaagaa attaaagcaa gataggctat
     4261 taagtgcaga gggagagaaa atgcctccaa catgtgagga agtaatgaga gaaatcatag
     4321 aattttaagg ccatgattta aggccaatcg gaaagaacat gtgagcaaaa ggccagcaaa
     4381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcccaatga
     6181 ttgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcaga
     6241 ttggctattg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
     6301 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
     6361 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     6421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     6481 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     6541 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     6601 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     6661 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     6721 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     6781 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     6841 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     6901 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     6961 tccatagaag acaccgggac cgatccagcc tccatcggct cgcatctctc cttcacgcgc
     7021 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     7081 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     7141 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     7201 cctgaccctg cttgctcaac tctagttaac ggtggagggc agtgtagtct gagcagtact
     7261 cgttgctgcc gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc
     7321 catgggtctt ttctgcagtc accgtcgtcg acacgtgtga tcagatatcg cggccgctct
     7381 aga
//
