LOCUS       sequence-486224-        7377 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (BA.5-2a) protein for creating 
            pseudotyped virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (BA.5-2a)
FEATURES             Location/Qualifiers
     source          1..7377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3773..3997
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4412..5000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4492..4511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5171..5962,5963..6031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5794..5813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6032..6136)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6319..6698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6699..6902
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6853..6873
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6899..6923
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtgt ttctggttct tcttcccctg gtgtctagtc agtgcgtcaa cttgatcacg
       61 cgaacacaga gctacaccaa ttcctttaca cgaggcgtct actatcccga caaggtgttc
      121 cgaagcagtg ttttgcactc cacgcaggac cttttcttgc ccttcttctc aaatgtgact
      181 tggtttcatg caatatccgg gactaatggc actaagagat tcgacaatcc cgtgttgcct
      241 tttaacgacg gcgtgtattt tgccagtaca gaaaagagca atattatccg gggatggatc
      301 ttcggcacga ctctggatag taaaacacaa tccctgctga tcgtgaataa tgcgacaaac
      361 gtcgtcataa aagtctgcga gttccagttc tgcaacgacc ctttcctcga cgtgtattac
      421 cataagaaca ataaatcttg gatggaaagt gagtttcgcg tgtactccag tgcaaataac
      481 tgtacttttg agtacgtaag ccagcctttc ctgatggacc tcgaagggaa acaggggaac
      541 tttaagaacc tgcgcgaatt cgtgttcaag aacatcgacg gttattttaa aatatacagc
      601 aagcacaccc ctattaacct gggcagagat ttgccacaag gttttagcgc cctggaaccc
      661 ctcgtggacc ttcctatcgg catcaacatc acgcgcttcc aaactctgct cgcactgcat
      721 cggtcttatc tgacccctgg agattcatcc tcaggatgga ccgcgggtgc tgctgcatat
      781 tatgtgggat atctgcagcc taggaccttc ctgctcaaat acaacgagaa cggcaccatc
      841 actgacgctg ttgattgcgc gctggaccca ttgtctgaaa ccaaatgcac actgaaaagc
      901 ttcacagtcg agaagggcat ttaccagacg agcaacttca gagttcagcc cacagagagc
      961 atcgttcgct tccccaacat taccaatttg tgcccattcg acgaggtctt taacgcaact
     1021 acctttgcca gtgtttacgc ctggaacagg aaacggatat ccaattgcgt ggccgattac
     1081 agcgtactgt acaatttcgc tccctttttc gcctttaaat gctacggtgt ctcccctacg
     1141 aagctgaatg atctgtgctt tactaatgtc tatgcggaca gcttcgttat tagaggtaac
     1201 gaggtcagcc aaatcgcccc agggcagact ggaaacatag ccgactacaa ttacaaactg
     1261 ccagatgatt tcaccggctg cgtcatcgcc tggaactcca ataagctcga tagtaaagtg
     1321 ggcggcaatt acaactatcg gtataggctg tttcgaaaga gcaacctgaa accttttgag
     1381 cgggacattt caacagagat ctaccaggcc ggaaacaagc cctgcaacgg ggtcgccggg
     1441 gtgaattgtt acttcccgct gcaacggtat ggatttcggc cgacttacgg cgttggccac
     1501 cagccatatc gggtggtggt gctgtcattt gagctgcttc atgcgcctgc tacagtatgt
     1561 gggcctaaaa agtctaccaa tctggtgaaa aataagtgcg tgaacttcaa cttcaatggc
     1621 ctcaccggga caggcgtgtt gacagaatcc aataagaaat tcctgccatt ccagcagttc
     1681 ggcagggata tcgcagacac gaccgatgcg gtgcgagatc cccagacatt ggagattctg
     1741 gacattactc cttgttcttt tggaggagtg agtgttatta ctcccggcac aaatacctca
     1801 aatcaagtcg ctgtgctgta tcaaggtgta aactgtaccg aggtccccgt ggctattcac
     1861 gccgaccagc tgaccccaac atggcgggta tacagcaccg gttctaatgt attccagacc
     1921 agagccggct gcctcattgg agccgagtac gtgaacaact cttatgaatg tgatataccg
     1981 attggagcag gtatctgcgc ctcttatcag acccagacaa agagccacag aagggctagg
     2041 agcgtggcct cccagtccat aatcgcctat acaatgtcac tgggggctga aaattcagtg
     2101 gcatacagta acaactctat cgctatcccc acaaatttta ccatctctgt gactacagag
     2161 atcctgccag tgtctatgac taaaacctcc gtggactgta ccatgtacat ctgtggtgat
     2221 agcaccgaat gttccaacct gctcctgcag tacggcagct tctgcacaca gctgaagagg
     2281 gcactgacag gaatcgccgt cgaacaggat aagaacactc aggaggtgtt tgcgcaggtt
     2341 aagcagattt acaagacgcc accaatcaag tacttcggtg ggttcaattt ctctcaaatt
     2401 ctgcctgatc cttctaagcc atctaagcgg agctttatag aggatttgct ctttaataag
     2461 gtgaccctcg ccgatgctgg atttattaag cagtatggcg actgtttggg agacattgct
     2521 gctcgcgatc tgatatgtgc ccagaaattc aacgggctga ccgtactccc accgctgctc
     2581 actgatgaga tgatcgcaca atataccagc gccctgctgg ccggcactat tacatccggc
     2641 tggacgttcg gagcaggagc cgcactgcaa ataccttttg ccatgcagat ggcatatcgc
     2701 ttcaatggga tcggggtgac ccagaacgtc ctgtacgaaa accagaaact tatcgctaac
     2761 cagttcaatt ccgccattgg taaaatccag gacagcttga gttccacagc cagcgccctc
     2821 gggaaattgc aagatgtagt caatcacaat gcacaagccc tgaatactct cgtcaagcaa
     2881 ctgtcatcca agttcggtgc tatatcctcc gtgctgaatg atatccttag tagattggat
     2941 aaggtggagg cagaagtcca gatcgaccgg cttattactg gaaggctgca gagtctgcag
     3001 acgtacgtca cgcagcagct tatcagagcc gccgagatta gagcatctgc gaatctggct
     3061 gctacaaaga tgagtgaatg cgtgctcggg caatcaaagc gggtcgactt ttgtgggaag
     3121 gggtaccacc tcatgagctt cccccaatcc gccccacatg gcgttgtgtt cctgcatgtt
     3181 acttacgttc ccgctcaaga gaagaacttt actaccgctc cggcgatctg ccacgacggt
     3241 aaggcacact ttccacgcga aggcgtcttt gttagcaatg gaacacactg gtttgttacc
     3301 cagaggaact tttatgagcc ccagataatc acaaccgaca acacattcgt atctgggaac
     3361 tgtgacgtag ttattgggat cgtgaacaac accgtgtacg atcctctcca accggaactt
     3421 gactctttca aggaggaact ggataagtac tttaagaatc acacctcacc cgatgttgac
     3481 ctcggcgaca tttcaggaat taatgctagc gtggtgaaca tccagaaaga gattgataga
     3541 cttaacgaag tggccaagaa cctcaacgaa tcacttattg acctgcagga gctgggaaaa
     3601 tatgagcagt acattaaatg gccttggtac atttggctgg gctttatagc aggcctgatc
     3661 gctatcgtca tggtgaccat catgctttgt tgtatgacta gctgctgcag ctgcctcaag
     3721 ggctgttgta gctgcggctc ttgctgtaaa taatgatgag gatccagatc tgctgtgcct
     3781 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3841 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3901 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3961 aatagcaggc atgctgggga tgcggtgggc tctatgggta cccaggtgct gaagaattga
     4021 cccggttcct cctgggccag aaagaagcag gcacatcccc ttctctgtga cacaccctgt
     4081 ccacgcccct ggttcttagt tccagcccca ctcataggac actcatagct caggagggct
     4141 ccgccttcaa tcccacccgc taaagtactt ggagcggtct ctccctccct catcagccca
     4201 ccaaaccaaa cctagcctcc aagagtggga agaaattaaa gcaagatagg ctattaagtg
     4261 cagagggaga gaaaatgcct ccaacatgtg aggaagtaat gagagaaatc atagaatttt
     4321 aaggccatga tttaaggcca atcggaaaga acatgtgagc aaaaggccag caaaaggcca
     4381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4681 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4741 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4801 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4861 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4921 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4981 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5041 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5101 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5281 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6061 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6121 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtccca atgattgcac
     6181 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat cagattggct
     6241 attggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
     6301 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     6361 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     6421 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     6481 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     6541 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     6601 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     6661 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     6721 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     6781 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     6841 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     6901 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     6961 gaagacaccg ggaccgatcc agcctccatc ggctcgcatc tctccttcac gcgcccgccg
     7021 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     7081 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     7141 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     7201 cctgcttgct caactctagt taacggtgga gggcagtgta gtctgagcag tactcgttgc
     7261 tgccgcgcgc gccaccagac ataatagctg acagactaac agactgttcc tttccatggg
     7321 tcttttctgc agtcaccgtc gtcgacacgt gtgatcagat atcgcggccg ctctaga
//
