LOCUS       sequence-486230-        7377 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (BA.5-4a) protein for creating 
            pseudotyped virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (BA.5-4a)
FEATURES             Location/Qualifiers
     source          1..7377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3773..3997
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4412..5000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4492..4511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5171..5962,5963..6031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5794..5813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6032..6136)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6319..6698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6699..6902
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6853..6873
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6899..6923
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtgt ttctggttct gcttcccctg gtctctagtc agtgcgtcaa ccttatcacc
       61 cgaacacaga gttataccaa ttcctttacg agaggtgttt actatcccga taaagtgttc
      121 cggagcagtg ttctgcactc cactcaggac ctcttcctgc ccttcttttc aaacgttaca
      181 tggtttcacg caatcagtgg gactaacggc atcaagaggt tcgacaaccc cgtgttgcct
      241 ttcaacgacg gcgtgtattt tgccagtacc gaaaagagca atatcatccg gggatggatc
      301 ttcggcacga cccttgatag taagacacaa agcctgctca tagtgaataa tgcaacgaac
      361 gtcgtaatca aggtgtgcga gttccagttt tgcaatgacc cttttctgga cgtgtattac
      421 cataagaaca ataaatcttg gagcgaaagt gagttccggg tgtactccag cgcaaataac
      481 tgtacttttg agtatgtgag ccagccgttt ctgatggacc tcgaaggcaa acagggcaat
      541 ttcaaaaacc tgcgcgaatt tgtgttcaag aacatcgacg gttatttcaa gatctactcc
      601 aagcataccc ctattaacct gggtcgcgat ctgccacaag gcttttcagc cctcgaacct
      661 ctcgtggatt tgccgattgg cataaacatt acccgcttcc aaacattgct cgcactgcat
      721 cggtcttatc tgaccccagg agactcatcc agcggatgga ctgccggggc tgctgcatat
      781 tacgtcgggt acctgcagcc gaggactttc ctgctgaagt acaatgagaa cggcactatt
      841 accgacgctg ttgattgcgc gctggaccca ttgtctgaaa ccaaatgtac actgaaaagt
      901 ttcacagtcg agaaagggat ctatcagaca tcaaatttca gggttcagcc cacagagagc
      961 attgttagat tccccaacat caccaacttg tgcccattcg atgaggtctt taatgctacc
     1021 aggttcgcca gcgtgtatgc gtggaatagg aaaagaatca gcaattgcgt ggccgattac
     1081 agcgtgctgt acaacttcgc gcccttcttc gcattcaaat gctacggcgt gtcccctacc
     1141 aagctcaacg atctgtgttt tactaacgtt tacgccgata gcttcgtgat tagggggaat
     1201 gaggtaagcc aaatcgcacc tggccagacc ggaaatattg cagactataa ttacaagctt
     1261 cccgatgatt tcaccgggtg cgtcatcgcg tggaactcca ataagctcga ttcaaaagtg
     1321 ggaggtaact ataactaccg gtacaggttg tttcgaaagt ccaacctgaa gccttttgag
     1381 cgggacattt caaccgagat tttccaggcc ggaaataaac cctgcaacgg ggtcgcaggg
     1441 gtgaattgtt acttccctct tcggtcttac ggatttcgcc cgacatacgg ggttggccac
     1501 cagccatacc gggtggtagt gctttcattt gagctgcttc atgcccctgc tacagtttgt
     1561 ggccctaaga agtctaccaa ccttgttaaa aataagtgcg tgaactttaa tttcaacggt
     1621 ctcactggta ctggagttct cacggaatcc aacaagaaat tcttgccttt ccagcagttt
     1681 ggcagggata ttgcagacac taccgacgcc gtccgagatc cccagacact ggagatactg
     1741 gacataactc cttgttcttt tggcggagtt tcagttatta cccccggtac gaacaccagt
     1801 aatcaagtcg ctgtgctgta ccagggcgta aattgcaccg aggtccccgt cgctattcac
     1861 gcagaccagc tcactccaac atggcgcgtc tactctactg gaagtaacgt gttccagact
     1921 agagccggct gcctcattgg agccgagtac gttaacaatt cttatgaatg tgatatcccg
     1981 ataggagcag gtatctgcgc atcttatcag actcagacaa agtcccacag aagagctagg
     2041 tcagtggcgt cccagtccat cattgcctat acaatgagcc tgggggctga aaactccgtg
     2101 gcctatagca acaactctat agctatcccc actaatttta ccatctctgt aaccacagag
     2161 atcttgccag tctctatgac aaaaacgtcc gtcgattgta cgatgtacat ctgcggggat
     2221 agcaccgaat gctccaatct tttgctgcag tatggcagtt tctgtacaca gctgaaacga
     2281 gccctgacag ggattgcggt ggaacaggat aagaacaccc aagaagtgtt tgcgcaggtg
     2341 aaacagattt acaaaacccc accaataaag tattttggtg ggttcaattt ctctcaaatc
     2401 ctgcctgatc cttctaaacc tagcaaacgg agctttatag aggatctgct ctttaataag
     2461 gtcacgctcg ctgatgctgg atttattaaa cagtatggcg attgtctggg cgacattgct
     2521 gctcgcgatc tgatctgtgc ccagaagttc aatggtctca cggtactgcc tccacttctg
     2581 accgatgaga tgattgccca atataccagt gccctgcttg ccggcaccat tacatccgga
     2641 tggacattcg gtgccggagc cgcactgcaa atcccctttg caatgcagat ggcttatcgc
     2701 tttaatggga ttggggtgac tcagaacgtg ctgtacgaaa accagaaact gatagccaac
     2761 cagttcaatt ccgctattgg caagatacag gactccctga gctccacagc gtcagcgctc
     2821 gggaagctgc aagacgtggt caatcacaac gcacaggcct tgaacaccct cgtcaagcaa
     2881 ctgagtagca agttcggcgc cataagctca gtgctgaatg acatccttag tagactggat
     2941 aaagtcgagg ccgaagtaca gatcgaccgg ctgattaccg gacgattgca gtccctccag
     3001 acttacgtca cacagcagct gataagagcc gcagagatta gggcctctgc aaatttggct
     3061 gctacaaaaa tgtccgaatg cgtcctgggg caatcaaagc gcgtagactt ttgtggcaag
     3121 ggctaccacc tgatgtcatt cccacaatcc gccccacacg gtgttgtgtt tctgcatgtt
     3181 acttacgtgc ccgctcaaga gaagaatttt accactgccc ccgccatctg ccatgacggc
     3241 aaggcccatt ttccaagaga aggggtcttc gtgagcaatg gaacgcactg gttcgtgact
     3301 cagagaaact tttatgagcc ccagatcatt acaactgaca acacatttgt atctggcaat
     3361 tgtgacgtgg tgattgggat agtgaataac accgtgtacg atccactgca accggaactc
     3421 gactctttca aggaggaact ggacaagtac ttcaagaatc acaccagccc cgatgtggac
     3481 ctgggtgaca tcagtggaat caacgctagc gtagtgaata tccagaaaga gatcgacaga
     3541 ttgaacgaag tcgccaagaa cctgaacgag tcactgattg acctgcagga gttgggaaaa
     3601 tacgagcagt acattaagtg gccatggtat atctggctcg ggtttatcgc cggactcatc
     3661 gctatcgtaa tggtgaccat catgctttgt tgtatgacta gctgctgcag ctgccttaaa
     3721 ggctgttgta gctgcggctc ttgctgtaaa taatgatgag gatccagatc tgctgtgcct
     3781 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3841 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3901 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3961 aatagcaggc atgctgggga tgcggtgggc tctatgggta cccaggtgct gaagaattga
     4021 cccggttcct cctgggccag aaagaagcag gcacatcccc ttctctgtga cacaccctgt
     4081 ccacgcccct ggttcttagt tccagcccca ctcataggac actcatagct caggagggct
     4141 ccgccttcaa tcccacccgc taaagtactt ggagcggtct ctccctccct catcagccca
     4201 ccaaaccaaa cctagcctcc aagagtggga agaaattaaa gcaagatagg ctattaagtg
     4261 cagagggaga gaaaatgcct ccaacatgtg aggaagtaat gagagaaatc atagaatttt
     4321 aaggccatga tttaaggcca atcggaaaga acatgtgagc aaaaggccag caaaaggcca
     4381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4681 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4741 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4801 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4861 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4921 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4981 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5041 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5101 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5281 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6061 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6121 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtccca atgattgcac
     6181 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat cagattggct
     6241 attggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
     6301 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     6361 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     6421 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     6481 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     6541 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     6601 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     6661 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     6721 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     6781 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     6841 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     6901 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     6961 gaagacaccg ggaccgatcc agcctccatc ggctcgcatc tctccttcac gcgcccgccg
     7021 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     7081 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     7141 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     7201 cctgcttgct caactctagt taacggtgga gggcagtgta gtctgagcag tactcgttgc
     7261 tgccgcgcgc gccaccagac ataatagctg acagactaac agactgttcc tttccatggg
     7321 tcttttctgc agtcaccgtc gtcgacacgt gtgatcagat atcgcggccg ctctaga
//
