LOCUS       sequence-486236-        7386 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (Delta) protein for creating pseudotyped 
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (Delta)
FEATURES             Location/Qualifiers
     source          1..7386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3782..4006
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4421..5009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4501..4520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5180..5971,5972..6040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5803..5822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6041..6145)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6328..6707
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6708..6911
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6862..6882
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6908..6932
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgtttgtct ttttggttct ccttcccctt gtgtctagtc aatgtgtcaa cttgcggact
       61 cgaacacagc tgcccccagc gtacaccaat tcctttacaa ggggcgtata ctatcccgac
      121 aaagttttca gatccagcgt tttgcatagc acccaggacc tgtttctgcc cttcttctca
      181 aatgtgacat ggtttcacgc aatccacgtg tcagggacca acggtacaaa gaggttcgac
      241 aaccccgtct tgccgttcaa cgacggtgtg tatttcgcca gtacggaaaa aagcaatatc
      301 attcgcggat ggatattcgg cacgaccctg gatagtaaga cacaatcact tctgatcgtc
      361 aataatgcaa cgaacgtcgt gatcaaggtg tgcgagttcc agttttgcaa tgaccctttc
      421 ctggacgtat attaccataa gaacaacaaa tcttggatgg aaagtggcgt gtattccagt
      481 gctaacaatt gtacttttga gtacgtctcc cagcccttcc ttatggacct cgaaggtaaa
      541 cagggcaatt ttaagaacct gcgggaattc gtgttcaaaa atatcgacgg ttatttcaag
      601 atatattcaa agcacacccc gatcaacctt gtccgagatc tgcctcaagg atttagtgcg
      661 ctggaacctt tggtcgatct gcctatcggc attaatataa cgcgcttcca aacactgctc
      721 gcactgcatc gctcttacct gactccagga gattcatcct ccggatggac cgccggcgcc
      781 gctgcatatt acgtcggcta cctgcagcca agaacttttc tgctgaagta caacgagaat
      841 ggaactatca ccgacgctgt tgattgcgcg ctcgacccat tgtctgaaac caaatgcacg
      901 ttgaaaagct tcacagtcga gaaggggatt tatcagacaa gtaacttcag agttcagccc
      961 acagagtcaa tcgttagatt ccccaacatc accaatttgt gcccattcgg ggaggtgttt
     1021 aatgcaacca gatttgcctc cgtatacgcg tggaatagga aaaggatctc caattgcgtt
     1081 gccgattaca gcgtgctgta caacagcgcc agtttcagca ccttcaaatg ctacggtgtg
     1141 tccccaacca agctgaatga tctctgcttc actaacgttt acgcagatag cttcgtcatt
     1201 agaggcgacg aggtgcgaca aattgcccct gggcagaccg gaaagattgc agactacaat
     1261 tacaagctgc cagatgattt taccggatgc gtgatcgctt ggaactccaa taacctcgat
     1321 agcaaagtgg gtggcaacta taactatcgg tacaggctgt ttcgaaagtc aaatctgaaa
     1381 ccgtttgagc gcgacattag caccgagatt taccaggccg gaagtaaacc ctgcaacggg
     1441 gtcgagggtt tcaattgtta cttcccgctg caaagctacg gatttcagcc tacaaacggc
     1501 gttgggtacc agccatatcg ggtggtcgtt ctgtcattcg agttgcttca tgcacctgct
     1561 acagtgtgtg ggcccaagaa gtctaccaac ctggttaaaa ataagtgcgt gaacttcaac
     1621 tttaatggcc tcacgggcac gggggtgttg actgaatcca acaaaaaatt cctgccgttt
     1681 cagcagtttg ggagggatat tgcggacact actgacgccg tgcgagatcc ccagacactc
     1741 gagattctgg acataactcc ctgttctttt gggggagtga gtgttattac tcccggcaca
     1801 aataccagca atcaagtcgc tgtcctctat caaggcgtaa attgcaccga ggtccccgtg
     1861 gctatacacg ctgatcagct caccccaaca tggcgggtgt acagcaccgg ctccaacgtc
     1921 ttccagacga gagcgggatg cctcatcgga gccgagcatg tcaacaattc ttatgaatgt
     1981 gatatcccta taggagcagg gatttgtgct tcttatcaga cccagacaaa ctcacgccgg
     2041 agggctagga gcgtcgcctc ccagtccatc atcgcatata caatgagcct cggggctgaa
     2101 aactctgtcg cctacagcaa caattctatc gctataccca ctaattttac tatctctgta
     2161 accacggaga tcctgccagt gtctatgaca aaaaccagtg ttgattgtac gatgtacatt
     2221 tgtggcgata gcactgaatg ctccaacttg cttctgcagt acggttcatt ttgtacacag
     2281 ctgaacagag ccctgacagg aatcgcagtg gaacaggata aaaacaccca ggaggtattt
     2341 gcccaagtga aacagatcta caagactcct ccaatcaaag acttcggtgg gttcaatttt
     2401 tctcaaatcc tgcctgatcc atctaagcct tcaaagcgga gttttataga ggatctgctc
     2461 tttaataagg tgaccctcgc cgatgctgga tttataaaac agtatggcga ctgtctgggc
     2521 gacattgctg ctcgggatct tatctgtgcg cagaaattca acgggctgac cgtactgcct
     2581 ccactgctga ccgatgagat gattgcccaa tacacctctg cactgctcgc gggcactatt
     2641 acatccggct ggacattcgg cgcaggagcc gcactgcaaa ttccttttgc catgcagatg
     2701 gcctatcggt ttaatggcat aggggtgact cagaacgtgc tgtatgaaaa tcagaagctg
     2761 atcgccaacc agtttaattc cgccattgga aagatccagg actcccttag ctccacagcc
     2821 agtgccctcg ggaagctgca aaacgtggtc aatcagaatg cgcaggccct caacaccctg
     2881 gtgaagcaac tgtccagcaa cttcggtgcc ataagcagcg tgcttaatga cattcttagt
     2941 agactcgata aggtggaagc agaagtccag atcgaccgcc tgattaccgg acggcttcag
     3001 agcttgcaga cctatgtcac acagcagctg attagggccg ccgagattag agcatctgcc
     3061 aatctggctg ctactaaaat gagcgaatgc gtgctggggc agtcaaagcg cgtggacttt
     3121 tgtgggaagg gctaccacct gatgagtttc ccacaatccg caccacatgg cgttgtgttc
     3181 cttcacgtta cttacgtgcc cgctcaagag aagaacttta ccactgcacc tgccatctgc
     3241 cacgacggca aggctcactt cccaagggaa ggtgtgttcg tgagcaatgg aacacactgg
     3301 ttcgtaactc agagaaactt ctatgagccc cagattatta caaccgacaa cacattcgta
     3361 tctggtaact gtgatgtggt gattgggatc gttaacaata ccgtttatga ccctctccaa
     3421 ccggaactgg actcttttaa agaggaactg gacaagtatt tcaagaatca tactagcccc
     3481 gatgtggact tgggcgacat atcaggaatt aacgctagcg tggtaaatat ccagaaagag
     3541 attgaccgct tgaatgaagt ggccaagaac ctgaacgaga gcctcatcga cctgcaggag
     3601 ctcggaaaat acgagcagta tatcaagtgg ccttggtaca tctggctggg ttttatagcc
     3661 ggcctgatcg ctattgtaat ggtgactatc atgctgtgtt gcatgacctc atgctgctcc
     3721 tgccttaagg ggtgttgctc atgcggctct tgttgtaaat aatgatgagg atccagatct
     3781 gctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3841 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3901 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3961 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatgggtac ccaggtgctg
     4021 aagaattgac ccggttcctc ctgggccaga aagaagcagg cacatcccct tctctgtgac
     4081 acaccctgtc cacgcccctg gttcttagtt ccagccccac tcataggaca ctcatagctc
     4141 aggagggctc cgccttcaat cccacccgct aaagtacttg gagcggtctc tccctccctc
     4201 atcagcccac caaaccaaac ctagcctcca agagtgggaa gaaattaaag caagataggc
     4261 tattaagtgc agagggagag aaaatgcctc caacatgtga ggaagtaatg agagaaatca
     4321 tagaatttta aggccatgat ttaaggccaa tcggaaagaa catgtgagca aaaggccagc
     4381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4861 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5101 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5161 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5461 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5761 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcccaa
     6181 tgattgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     6241 agattggcta ttggccattg catacgttgt atccatatca taatatgtac atttatattg
     6301 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
     6361 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     6421 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     6481 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     6541 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     6601 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     6661 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     6721 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6781 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6841 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6901 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     6961 acctccatag aagacaccgg gaccgatcca gcctccatcg gctcgcatct ctccttcacg
     7021 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     7081 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     7141 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     7201 ttgcctgacc ctgcttgctc aactctagtt aacggtggag ggcagtgtag tctgagcagt
     7261 actcgttgct gccgcgcgcg ccaccagaca taatagctga cagactaaca gactgttcct
     7321 ttccatgggt cttttctgca gtcaccgtcg tcgacacgtg tgatcagata tcgcggccgc
     7381 tctaga
//
