LOCUS       sequence-486237-        7380 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (EG.5) protein for creating pseudotyped 
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (EG.5)
FEATURES             Location/Qualifiers
     source          1..7380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3776..4000
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4415..5003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4495..4514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5174..5965,5966..6034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5797..5816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6035..6139)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6322..6701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6702..6905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6856..6876
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6902..6926
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtgt ttctggttct tcttcccctg gtgtctagtc agtgcgtcaa cttgatcacg
       61 cgaacacaga gctacaccaa ttcctttaca cgaggcgtct actatcccga caaggtgttc
      121 cgaagcagtg ttttgcactc cacgcaggac cttttcttgc ccttcttctc aaatgtgact
      181 tggtttcatg caatacacgt atccgggact aatggcacta agagattcga caatcccgcc
      241 ttgcctttta acgacggcgt gtattttgcc agtacagaaa agagcaatat tatccgggga
      301 tggatcttcg gcacgactct ggatagtaaa acacaatccc tgctgatcgt gaataatgcg
      361 acaaacgtcg tcataaaagt ctgcgagttc cagttctgca acgacccttt cctcgacgtg
      421 taccagaaga acaataaatc ttggatggaa agtgagtttc gcgtgtactc cagtgcaaat
      481 aactgtactt ttgagtacgt aagccagcct ttcctgatgg acctcgaagg gaaagagggg
      541 aactttaaga acctgcgcga attcgtgttc aagaacatcg acggttattt taaaatatac
      601 agcaagcaca cccctattaa cctggagaga gatttgccac aaggttttag cgccctggaa
      661 cccctcgtgg accttcctat cggcatcaac atcacgcgct tccaaactct gctcgcactg
      721 catcggtctt atctgacccc tgtggattca tcctcaggat ggaccgcggg tgctgctgca
      781 tattatgtgg gatatctgca gcctaggacc ttcctgctca aatacaacga gaacggcacc
      841 atcactgacg ctgttgattg cgcgctggac ccattgtctg aaaccaaatg cacactgaaa
      901 agcttcacag tcgagaaggg catttaccag acgagcaact tcagagttca gcccacagag
      961 agcatcgttc gcttccccaa cattaccaat ttgtgcccat tccacgaggt ctttaacgca
     1021 actacctttg ccagtgttta cgcctggaac aggaaacgga tatccaattg cgtggccgat
     1081 tacagcgtaa tctacaattt cgctcccttt ttcgccttta aatgctacgg tgtctcccct
     1141 acgaagctga atgatctgtg ctttactaat gtctatgcgg acagcttcgt tattagaggt
     1201 aacgaggtca gccaaatcgc cccagggcag actggaaaca tagccgacta caattacaaa
     1261 ctgccagatg atttcaccgg ctgcgtcatc gcctggaact ccaataagct cgatagtaaa
     1321 cccagcggca attacaacta tctctatagg ctgctgcgaa agagcaagct gaaacctttt
     1381 gagcgggaca tttcaacaga gatctaccag gccggaaaca agccctgcaa cggggtcgcc
     1441 gggcccaatt gttacagccc gctgcaaagc tatggatttc ggccgactta cggcgttggc
     1501 caccagccat atcgggtggt ggtgctgtca tttgagctgc ttcatgcgcc tgctacagta
     1561 tgtgggccta aaaagtctac caatctggtg aaaaataagt gcgtgaactt caacttcaat
     1621 ggcctcaccg ggacaggcgt gttgacagaa tccaataaga aattcctgcc attccagcag
     1681 ttcggcaggg atatcgcaga cacgaccgat gcggtgcgag atccccagac attggagatt
     1741 ctggacatta ctccttgttc ttttggagga gtgagtgtta ttactcccgg cacaaatacc
     1801 tcaaatcaag tcgctgtgct gtatcaaggt gtaaactgta ccgaggtccc cgtggctatt
     1861 cacgccgacc agctgacccc aacatggcgg gtatacagca ccggttctaa tgtattccag
     1921 accagagccg gctgcctcat tggagccgag tacgtgaaca actcttatga atgtgatata
     1981 ccgattggag caggtatctg cgcctcttat cagacccaga caaagagcca cagaagggct
     2041 aggagcgtgg cctcccagtc cataatcgcc tatacaatgt cactgggggc tgaaaattca
     2101 gtggcataca gtaacaactc tatcgctatc cccacaaatt ttaccatctc tgtgactaca
     2161 gagatcctgc cagtgtctat gactaaaacc tccgtggact gtaccatgta catctgtggt
     2221 gatagcaccg aatgttccaa cctgctcctg cagtacggca gcttctgcac acagctgaag
     2281 agggcactga caggaatcgc cgtcgaacag gataagaaca ctcaggaggt gtttgcgcag
     2341 gttaagcaga tttacaagac gccaccaatc aagtacttcg gtgggttcaa tttctctcaa
     2401 attctgcctg atccttctaa gccatctaag cggagcttta tagaggattt gctctttaat
     2461 aaggtgaccc tcgccgatgc tggatttatt aagcagtatg gcgactgttt gggagacatt
     2521 gctgctcgcg atctgatatg tgcccagaaa ttcaacgggc tgaccgtact cccaccgctg
     2581 ctcactgatg agatgatcgc acaatatacc agcgccctgc tggccggcac tattacatcc
     2641 ggctggacgt tcggagcagg agccgcactg caaatacctt ttgccatgca gatggcatat
     2701 cgcttcaatg ggatcggggt gacccagaac gtcctgtacg aaaaccagaa acttatcgct
     2761 aaccagttca attccgccat tggtaaaatc caggacagct tgagttccac agccagcgcc
     2821 ctcgggaaat tgcaagatgt agtcaatcac aatgcacaag ccctgaatac tctcgtcaag
     2881 caactgtcat ccaagttcgg tgctatatcc tccgtgctga atgatatcct tagtagattg
     2941 gataaggtgg aggcagaagt ccagatcgac cggcttatta ctggaaggct gcagagtctg
     3001 cagacgtacg tcacgcagca gcttatcaga gccgccgaga ttagagcatc tgcgaatctg
     3061 gctgctacaa agatgagtga atgcgtgctc gggcaatcaa agcgggtcga cttttgtggg
     3121 aaggggtacc acctcatgag cttcccccaa tccgccccac atggcgttgt gttcctgcat
     3181 gttacttacg ttcccgctca agagaagaac tttactaccg ctccggcgat ctgccacgac
     3241 ggtaaggcac actttccacg cgaaggcgtc tttgttagca atggaacaca ctggtttgtt
     3301 acccagagga acttttatga gccccagata atcacaaccg acaacacatt cgtatctggg
     3361 aactgtgacg tagttattgg gatcgtgaac aacaccgtgt acgatcctct ccaaccggaa
     3421 cttgactctt tcaaggagga actggataag tactttaaga atcacacctc acccgatgtt
     3481 gacctcggcg acatttcagg aattaatgct agcgtggtga acatccagaa agagattgat
     3541 agacttaacg aagtggccaa gaacctcaac gaatcactta ttgacctgca ggagctggga
     3601 aaatatgagc agtacattaa atggccttgg tacatttggc tgggctttat agcaggcctg
     3661 atcgctatcg tcatggtgac catcatgctt tgttgtatga ctagctgctg cagctgcctc
     3721 aagggctgtt gtagctgcgg ctcttgctgt aaataatgat gaggatccag atctgctgtg
     3781 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3841 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3901 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3961 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg gtacccaggt gctgaagaat
     4021 tgacccggtt cctcctgggc cagaaagaag caggcacatc cccttctctg tgacacaccc
     4081 tgtccacgcc cctggttctt agttccagcc ccactcatag gacactcata gctcaggagg
     4141 gctccgcctt caatcccacc cgctaaagta cttggagcgg tctctccctc cctcatcagc
     4201 ccaccaaacc aaacctagcc tccaagagtg ggaagaaatt aaagcaagat aggctattaa
     4261 gtgcagaggg agagaaaatg cctccaacat gtgaggaagt aatgagagaa atcatagaat
     4321 tttaaggcca tgatttaagg ccaatcggaa agaacatgtg agcaaaaggc cagcaaaagg
     4381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     4861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     5521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc ccaatgattg
     6181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcagattg
     6241 gctattggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
     6301 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
     6361 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     6421 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     6481 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     6541 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     6601 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     6661 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     6721 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     6781 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     6841 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     6901 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     6961 atagaagaca ccgggaccga tccagcctcc atcggctcgc atctctcctt cacgcgcccg
     7021 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     7081 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     7141 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     7201 gaccctgctt gctcaactct agttaacggt ggagggcagt gtagtctgag cagtactcgt
     7261 tgctgccgcg cgcgccacca gacataatag ctgacagact aacagactgt tcctttccat
     7321 gggtcttttc tgcagtcacc gtcgtcgaca cgtgtgatca gatatcgcgg ccgctctaga
//
